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Protein

Transcriptional regulatory protein RcsB

Gene

rcsB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.UniRule annotation7 Publications

Miscellaneous

There is a close linkage between the Rcs and PhoQ/P signaling systems, and both signaling systems respond to certain external divalent cations (zinc and magnesium).1 Publication

Enzyme regulationi

Activity is modulated by phosphorylation and interaction with other transcriptional regulators. Probably up-regulated by YmgA/AriR, and possibly down-regulated by YcgZ, all 3 are connector proteins providing additional signal input into the signaling system.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi168 – 187H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • phosphorelay signal transduction system Source: UniProtKB-KW
  • positive regulation of cell projection organization Source: CACAO
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • response to antibiotic Source: CACAO
  • single-species biofilm formation on inanimate substrate Source: CACAO
  • transcription, DNA-templated Source: EcoCyc

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCapsule biogenesis/degradation, Transcription, Transcription regulation, Two-component regulatory system

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein RcsBUniRule annotation
Alternative name(s):
Capsular synthesis regulator component B
Gene namesi
Name:rcsBUniRule annotation
Ordered Locus Names:b2217, JW2205
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10821. rcsB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56D → E: Increases heterodimer formation with RcsA. Does not affect heterodimer formation with BglJ. 1 Publication1
Mutagenesisi56D → N: Decreases heterodimer formation with RcsA. Does not affect heterodimer formation with BglJ. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000812091 – 216Transcriptional regulatory protein RcsBAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei564-aspartylphosphateUniRule annotation1

Post-translational modificationi

Phosphorylated and activated by RcsD.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0DMC7.
PRIDEiP0DMC7.

Expressioni

Inductioni

Expression is dependent on the alternate sigma factor, RpoN (PubMed:2404948). Repressed by hydroxyurea.2 Publications

Interactioni

Subunit structurei

Interacts with RcsD and RcsA. Homodimer. Can form an heterodimer with the coactivator RcsA, which binds to the RcsAB boxes to promote transcription of RcsA-dependent genes. Can also form a heterodimer with GadE to bind to the GAD box, and with BglJ to bind to the bgl promoter.UniRule annotation6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
bglJP394042EBI-369670,EBI-551429

Protein-protein interaction databases

BioGridi4260879. 3 interactors.
IntActiP0DMC7. 40 interactors.
STRINGi316385.ECDH10B_2375.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Helixi13 – 23Combined sources11
Beta strandi29 – 37Combined sources9
Helixi38 – 44Combined sources7
Helixi45 – 47Combined sources3
Beta strandi51 – 57Combined sources7
Turni62 – 64Combined sources3
Helixi67 – 77Combined sources11
Beta strandi82 – 89Combined sources8
Helixi92 – 99Combined sources8
Turni100 – 102Combined sources3
Beta strandi104 – 108Combined sources5
Helixi109 – 111Combined sources3
Helixi112 – 124Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5I4CX-ray2.00A1-147[»]
ProteinModelPortaliP0DMC7.
SMRiP0DMC7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 124Response regulatoryPROSITE-ProRule annotationAdd BLAST120
Domaini144 – 209HTH luxR-typeUniRule annotationAdd BLAST66

Sequence similaritiesi

Belongs to the RcsB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG41068CG. Bacteria.
COG2197. LUCA.
KOiK07687.
PhylomeDBiP0DMC7.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00981. RcsB. 1 hit.
InterProiView protein in InterPro
IPR011006. CheY-like_superfamily.
IPR030864. RcsB.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
PRINTSiPR00038. HTHLUXR.
SMARTiView protein in SMART
SM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiView protein in PROSITE
PS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.

Sequencei

Sequence statusi: Complete.

P0DMC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNMNVIIAD DHPIVLFGIR KSLEQIEWVN VVGEFEDSTA LINNLPKLDA
60 70 80 90 100
HVLITDLSMP GDKYGDGITL IKYIKRHFPS LSIIVLTMNN NPAILSAVLD
110 120 130 140 150
LDIEGIVLKQ GAPTDLPKAL AALQKGKKFT PESVSRLLEK ISAGGYGDKR
160 170 180 190 200
LSPKESEVLR LFAEGFLVTE IAKKLNRSIK TISSQKKSAM MKLGVENDIA
210
LLNYLSSVTL SPADKD
Length:216
Mass (Da):23,671
Last modified:February 19, 2014 - v1
Checksum:iA78D1BD3004E0680
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28242 Genomic DNA. Translation: AAA24504.1.
U00096 Genomic DNA. Translation: AAC75277.1.
AP009048 Genomic DNA. Translation: BAA16000.1.
S37760 Genomic DNA. Translation: AAB22290.1.
PIRiJV0068. BVECCB.
RefSeqiNP_416721.1. NC_000913.3.
WP_001061917.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75277; AAC75277; b2217.
BAA16000; BAA16000; BAA16000.
GeneIDi31658032.
947441.
KEGGiecj:JW2205.
eco:b2217.
PATRICifig|1411691.4.peg.18.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRCSB_ECOLI
AccessioniPrimary (citable) accession number: P0DMC7
Secondary accession number(s): P14374, P69407
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: February 19, 2014
Last modified: July 5, 2017
This is version 30 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families