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Protein

Clamp-binding protein CrfC

Gene

crfC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Important for the colocalization of sister nascent DNA strands after replication fork passage during DNA replication, and for positioning and subsequent partitioning of sister chromosomes. Does not have GTPase activity on its own.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Chromosome partition, DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Clamp-binding protein CrfC
Alternative name(s):
Clamp-binding sister replication fork colocalization protein
Gene namesi
Name:crfC
Synonyms:yjdA
Ordered Locus Names:b4109, JW4070
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11210. crfC.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: About half the protein co-localizes with beta sliding clamp (DnaN) at midcell, the rest without clamp in quarter-cell positions when chromosomes are condensed during DNA replication.

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

A small proportion of anucleate cells, disrupts nucleoid positioning, leads to premature separation of sister replication forks.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi23 – 231Q → A: Interacts with beta sliding clamp. 1 Publication
Mutagenesisi41 – 411Q → A: No longer interacts with beta sliding clamp, pattern of CrfC foci changes. 1 Publication
Mutagenesisi734 – 7341Q → A: Interacts with beta sliding clamp. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 742742Clamp-binding protein CrfCPRO_0000169728Add
BLAST

Proteomic databases

PaxDbiP0DM85.

Interactioni

Subunit structurei

Forms homooligomers. Binds to the beta sliding clamp processivity factor (DnaN) in the presence and absence of DNA, may bind to the clamp itself as homodimers or trimers. Homooligomers may be able to bind more than 1 clamp complex.1 Publication

Protein-protein interaction databases

BioGridi4263087. 5 interactions.
IntActiP0DM85. 12 interactions.
STRINGi511145.b4109.

Structurei

3D structure databases

ProteinModelPortaliP0DM85.
SMRiP0DM85. Positions 64-95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 402337Dynamin-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 455Clamp-binding consensus

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili440 – 47233Sequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4108F5E. Bacteria.
COG0699. LUCA.
OMAiTAGMATF.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00350. Dynamin_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0DM85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYTQTLYELS QEAERLLQLS RQQLQLLEKM PLSVPGDDAP QLALPWSQPN
60 70 80 90 100
IAERHAMLNN ELRKISRLEM VLAIVGTMKA GKSTTINAIV GTEVLPNRNR
110 120 130 140 150
PMTALPTLIR HTPGQKEPVL HFSHVAPIDC LIQQLQQRLR DCDIKHLTDV
160 170 180 190 200
LEIDKDMRAL MQRIENGVAF EKYYLGAQPI FHCLKSLNDL VRLAKALDVD
210 220 230 240 250
FPFSAYAAIE HIPVIEVEFV HLAGLESYPG QLTLLDTPGP NEAGQPHLQK
260 270 280 290 300
MLNQQLARAS AVLAVLDYTQ LKSISDEEVR EAILAVGQSV PLYVLVNKFD
310 320 330 340 350
QQDRNSDDAD QVRALISGTL MKGCITPQQI FPVSSMWGYL ANRARYELAN
360 370 380 390 400
NGKLPPPEQQ RWVEDFAHAA LGRRWRHADL ADLEHIRHAA DQLWEDSLFA
410 420 430 440 450
QPIQALLHAA YANASLYALR SAAHKLLNYA QQAREYLDFR AHGLNVACEQ
460 470 480 490 500
LRQNIHQIEE SLQLLQLNQA QVSGEIKHEI ELALTSANHF LRQQQDALKV
510 520 530 540 550
QLAALFQDDS EPLSEIRTRC ETLLQTAQNT ISRDFTLRFA ELESTLCRVL
560 570 580 590 600
TDVIRPIEQQ VKMELSESGF RPGFHFPVFH GVVPHFNTRQ LFSEVISRQE
610 620 630 640 650
ATDEQSTRLG VVRETFSRWL NQPDWGRGNE KSPTETVDYS VLQRALSAEV
660 670 680 690 700
DLYCQQMAKV LAEQVDESVT AGMNTFFAEF ASCLTELQTR LRESLALRQQ
710 720 730 740
NESVVRLMQQ QLQQTVMTHG WIYTDAQLLR DDIQTLFTAE RY
Length:742
Mass (Da):84,371
Last modified:December 11, 2013 - v1
Checksum:i8A7008FC16A026B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97008.1.
U00096 Genomic DNA. Translation: AAC77070.1.
AP009048 Genomic DNA. Translation: BAE78111.1.
PIRiS56337.
RefSeqiNP_418533.1. NC_000913.3.
WP_000288603.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77070; AAC77070; b4109.
BAE78111; BAE78111; BAE78111.
GeneIDi948620.
KEGGiecj:JW4070.
eco:b4109.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97008.1.
U00096 Genomic DNA. Translation: AAC77070.1.
AP009048 Genomic DNA. Translation: BAE78111.1.
PIRiS56337.
RefSeqiNP_418533.1. NC_000913.3.
WP_000288603.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0DM85.
SMRiP0DM85. Positions 64-95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263087. 5 interactions.
IntActiP0DM85. 12 interactions.
STRINGi511145.b4109.

Proteomic databases

PaxDbiP0DM85.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77070; AAC77070; b4109.
BAE78111; BAE78111; BAE78111.
GeneIDi948620.
KEGGiecj:JW4070.
eco:b4109.

Organism-specific databases

EchoBASEiEB1195.
EcoGeneiEG11210. crfC.

Phylogenomic databases

eggNOGiENOG4108F5E. Bacteria.
COG0699. LUCA.
OMAiTAGMATF.

Miscellaneous databases

PROiP0DM85.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00350. Dynamin_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "A replicase clamp-binding dynamin-like protein promotes colocalization of nascent DNA strands and equipartitioning of chromosomes in E. coli."
    Ozaki S., Matsuda Y., Keyamura K., Kawakami H., Noguchi Y., Kasho K., Nagata K., Masuda T., Sakiyama Y., Katayama T.
    Cell Rep. 4:985-995(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, LACK OF GTPASE, INTERACTION WITH DNAN, SUBUNIT, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLN-23; GLN-41 AND GLN-734.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiCRFC_ECOLI
AccessioniPrimary (citable) accession number: P0DM85
Secondary accession number(s): P16694, Q2M6J5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 11, 2013
Last sequence update: December 11, 2013
Last modified: January 20, 2016
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.