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Protein

Arsenate-mycothiol transferase ArsC2

Gene

arsC2

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in defense against toxic arsenate. Involved in the mycothiol/myoredoxin redox pathway which uses a mycothioltransferase mechanism; facilitates adduct formation between arsenate and mycothiol.1 Publication

Catalytic activityi

Arsenate + mycothiol = arseno-mycothiol + H2O.1 Publication

Kineticsi

  1. KM=142 mM for arsenate in the presence of mycoredoxin 1, mycothiol, mycothione reductase and NADPH1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Arsenical resistance

    Enzyme and pathway databases

    BRENDAi2.8.4.2. 960.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arsenate-mycothiol transferase ArsC2 (EC:2.8.4.2)
    Alternative name(s):
    Mycothiol-dependent arsenate reductase ArsC2
    Gene namesi
    Name:arsC2
    Synonyms:arsX
    Ordered Locus Names:cg0319, Cgl0263
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
    Proteomesi
    • UP000000582 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 129129Arsenate-mycothiol transferase ArsC2PRO_0000418725Add
    BLAST

    Expressioni

    Inductioni

    By arsenite.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi196627.cg0319.

    Structurei

    Secondary structure

    1
    129
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 1311Combined sources
    Helixi14 – 2512Combined sources
    Beta strandi30 – 389Combined sources
    Helixi45 – 539Combined sources
    Helixi68 – 736Combined sources
    Beta strandi75 – 839Combined sources
    Beta strandi94 – 996Combined sources
    Helixi108 – 12720Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RH0X-ray1.72A/B1-129[»]
    ProteinModelPortaliP0DKS7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0394. LUCA.
    KOiK18701.
    OMAiFEVMSAG.

    Family and domain databases

    InterProiIPR023485. Ptyr_pPase_SF.
    [Graphical view]
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0DKS7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKSVLFVCVG NGGKSQMAAA LAQKYASDSV EIHSAGTKPA QGLNQLSVES
    60 70 80 90 100
    IAEVGADMSQ GIPKAIDPEL LRTVDRVVIL GDDAQVDMPE SAQGALERWS
    110 120
    IEEPDAQGME RMRIVRDQID NRVQALLAG
    Length:129
    Mass (Da):13,763
    Last modified:January 9, 2013 - v1
    Checksum:i9279EF21FF0E52B8
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000036 Genomic DNA. Translation: BAB97656.1.
    BX927148 Genomic DNA. Translation: CAF18834.1.
    RefSeqiNP_599516.1. NC_003450.3.
    WP_003863342.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB97656; BAB97656; BAB97656.
    CAF18834; CAF18834; cg0319.
    GeneIDi1021323.
    KEGGicgb:cg0319.
    cgl:NCgl0259.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000036 Genomic DNA. Translation: BAB97656.1.
    BX927148 Genomic DNA. Translation: CAF18834.1.
    RefSeqiNP_599516.1. NC_003450.3.
    WP_003863342.1. NC_006958.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RH0X-ray1.72A/B1-129[»]
    ProteinModelPortaliP0DKS7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi196627.cg0319.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAB97656; BAB97656; BAB97656.
    CAF18834; CAF18834; cg0319.
    GeneIDi1021323.
    KEGGicgb:cg0319.
    cgl:NCgl0259.

    Phylogenomic databases

    eggNOGiCOG0394. LUCA.
    KOiK18701.
    OMAiFEVMSAG.

    Enzyme and pathway databases

    BRENDAi2.8.4.2. 960.

    Family and domain databases

    InterProiIPR023485. Ptyr_pPase_SF.
    [Graphical view]
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. "Analysis of genes involved in arsenic resistance in Corynebacterium glutamicum ATCC 13032."
      Ordonez E., Letek M., Valbuena N., Gil J.A., Mateos L.M.
      Appl. Environ. Microbiol. 71:6206-6215(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    4. "Arsenate reductase, mycothiol, and mycoredoxin concert thiol/disulfide exchange."
      Ordonez E., Van Belle K., Roos G., De Galan S., Letek M., Gil J.A., Wyns L., Mateos L.M., Messens J.
      J. Biol. Chem. 284:15107-15116(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    5. "Corynebacterium glutamicum survives arsenic stress with arsenate reductases coupled to two distinct redox mechanisms."
      Villadangos A.F., Van Belle K., Wahni K., Dufe V.T., Freitas S., Nur H., De Galan S., Gil J.A., Collet J.F., Mateos L.M., Messens J.
      Mol. Microbiol. 82:998-1014(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.72 ANGSTROMS).

    Entry informationi

    Entry nameiARSC2_CORGL
    AccessioniPrimary (citable) accession number: P0DKS7
    Secondary accession number(s): Q6M898, Q8NTP3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2013
    Last sequence update: January 9, 2013
    Last modified: January 20, 2016
    This is version 22 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.