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Protein

Disease resistance protein RRS1

Gene

RRS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Acts also as a disease resistance protein involved in resistance to fungal and bacterial pathogens, including R.solanacearum, P.syringae pv. tomato and C.higginsianum. Heterodimerization with RPS4 is required to form a functional complex to recognize AvrRps4 and PopP2 (PubMed:24744375). Contributes to temperature-conditioned RPS4 auto-immunity (PubMed:24146667).Curated5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi179 – 1868ATPSequence analysis
DNA bindingi1202 – 127069WRKYPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Plant defense, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Disease resistance protein RRS11 Publication
Alternative name(s):
Disease resistance protein RCH21 Publication
Disease resistance protein SLH1
Probable WRKY transcription factor 52
Protein RPS4-homolog1 Publication
Protein SENSITIVE TO LOW HUMIDITY 1
Resistance to Colletotrichum higginsianum 2 protein1 Publication
Resistance to Ralstonia solanacearum 1 protein1 Publication
WRKY DNA-binding protein 52
Gene namesi
Name:RRS11 Publication
Synonyms:RCH21 Publication, RRS1-S1 Publication, RSH41 Publication, SLH1, WRKY52
Ordered Locus Names:At5g45260/At5g45270
ORF Names:K9E15.2/K9E15.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G45260.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi25 – 251S → A: No effect on TIR domain homodimerization. Loss of TIR domain heterodimerization; when associated with A-33 in RPS4. 1 Publication
Mutagenesisi26 – 261H → A: No effect on TIR domain homodimerization. Loss of TIR domain heterodimerization with RPS4. 1 Publication
Mutagenesisi185 – 1851K → A: No effect on pathogen effectors triggered cell death. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12881288Disease resistance protein RRS1PRO_0000133693Add
BLAST

Proteomic databases

PaxDbiP0DKH5.

Expressioni

Gene expression databases

GenevisibleiP0DKH5. AT.

Interactioni

Subunit structurei

Interacts with PopP2, a R.solanacearum type III effector (PubMed:12788974). Interacts with RPS4 (PubMed:24744375).2 Publications

Protein-protein interaction databases

BioGridi19811. 1 interaction.
DIPiDIP-61676N.
MINTiMINT-267462.
STRINGi3702.AT5G45260.1.

Structurei

Secondary structure

1
1288
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 155Combined sources
Helixi17 – 204Combined sources
Helixi23 – 3311Combined sources
Beta strandi38 – 425Combined sources
Helixi47 – 515Combined sources
Helixi54 – 596Combined sources
Beta strandi61 – 688Combined sources
Helixi73 – 753Combined sources
Helixi77 – 804Combined sources
Helixi82 – 854Combined sources
Helixi86 – 905Combined sources
Beta strandi95 – 1017Combined sources
Helixi107 – 11610Combined sources
Beta strandi122 – 1243Combined sources
Helixi131 – 14616Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4C6SX-ray1.75A7-153[»]
4C6TX-ray2.65A/C6-153[»]
ProteinModelPortaliP0DKH5.
SMRiP0DKH5. Positions 8-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 145138TIRCuratedAdd
BLAST
Domaini170 – 421252NB-ARCSequence analysisAdd
BLAST
Repeati498 – 52225LRR 1Sequence analysisAdd
BLAST
Repeati535 – 55319LRR 2Sequence analysisAdd
BLAST
Repeati554 – 57522LRR 3Sequence analysisAdd
BLAST
Repeati577 – 59822LRR 4Sequence analysisAdd
BLAST
Repeati621 – 64626LRR 5Sequence analysisAdd
BLAST
Repeati665 – 68824LRR 6Sequence analysisAdd
BLAST
Repeati740 – 76425LRR 7Sequence analysisAdd
BLAST
Repeati766 – 79126LRR 8Sequence analysisAdd
BLAST
Repeati829 – 85224LRR 9Sequence analysisAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 262Important for interaction with RPS41 Publication

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi986 – 100318Nuclear localization signalPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi463 – 4664Poly-ArgSequence analysis
Compositional biasi1186 – 11905Poly-LysSequence analysis

Domaini

The TIR does not cause cell death, but can suppress RPS4 TIR domain-induced cell death (PubMed:24744375). The TIR domain is involved in homo- and heterodimerization, but other domains also contribute to the interaction (PubMed:24744375).1 Publication

Sequence similaritiesi

Contains 9 LRR (leucine-rich) repeats.Sequence analysis
Contains 1 NB-ARC domain.Sequence analysis
Contains 1 TIR domain.Curated
Contains 1 WRKY DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiENOG410JQXQ. Eukaryota.
ENOG410ZRAD. LUCA.
InParanoidiQ9FH83.
KOiK16225.
OMAiGNDATIM.
OrthoDBiEOG093611RA.
PhylomeDBiP0DKH5.

Family and domain databases

Gene3Di2.20.25.80. 1 hit.
3.40.50.300. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR011713. Leu-rich_rpt_3.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR000157. TIR_dom.
IPR011991. WHTH_DNA-bd_dom.
IPR003657. WRKY_dom.
[Graphical view]
PfamiPF07725. LRR_3. 1 hit.
PF00931. NB-ARC. 1 hit.
PF03106. WRKY. 1 hit.
[Graphical view]
SMARTiSM00774. WRKY. 1 hit.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 1 hit.
SSF46785. SSF46785. 1 hit.
SSF52058. SSF52058. 1 hit.
SSF52200. SSF52200. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50811. WRKY. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P0DKH5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNCEKDEEF VCISCVEEVR YSFVSHLSEA LRRKGINNVV VDVDIDDLLF
60 70 80 90 100
KESQAKIEKA GVSVMVLPGN CDPSEVWLDK FAKVLECQRN NKDQAVVSVL
110 120 130 140 150
YGDSLLRDQW LSELDFRGLS RIHQSRKECS DSILVEEIVR DVYETHFYVG
160 170 180 190 200
RIGIYSKLLE IENMVNKQPI GIRCVGIWGM PGIGKTTLAK AVFDQMSSAF
210 220 230 240 250
DASCFIEDYD KSIHEKGLYC LLEEQLLPGN DATIMKLSSL RDRLNSKRVL
260 270 280 290 300
VVLDDVRNAL VGESFLEGFD WLGPGSLIII TSRDKQVFCL CGINQIYEVQ
310 320 330 340 350
GLNEKEARQL FLLSASIKED MGEQNLQELS VRVINYANGN PLAISVYGRE
360 370 380 390 400
LKGKKKLSEM ETAFLKLKRR PPFKIVDAFK STYDTLSDNE KNIFLDIACF
410 420 430 440 450
FQGENVNYVI QLLEGCGFFP HVEIDVLVDK CLVTISENRV WLHKLTQDIG
460 470 480 490 500
REIINGETVQ IERRRRLWEP WSIKYLLEYN EHKANGEPKT TFKRAQGSEE
510 520 530 540 550
IEGLFLDTSN LRFDLQPSAF KNMLNLRLLK IYCSNPEVHP VINFPTGSLH
560 570 580 590 600
SLPNELRLLH WENYPLKSLP QNFDPRHLVE INMPYSQLQK LWGGTKNLEM
610 620 630 640 650
LRTIRLCHSH HLVDIDDLLK AENLEVIDLQ GCTRLQNFPA AGRLLRLRVV
660 670 680 690 700
NLSGCIKIKS VLEIPPNIEK LHLQGTGILA LPVSTVKPNH RELVNFLTEI
710 720 730 740 750
PGLSEELERL TSLLESNSSC QDLGKLICLE LKDCSCLQSL PNMANLDLNV
760 770 780 790 800
LDLSGCSSLN SIQGFPRFLK QLYLGGTAIR EVPQLPQSLE ILNAHGSCLR
810 820 830 840 850
SLPNMANLEF LKVLDLSGCS ELETIQGFPR NLKELYFAGT TLREVPQLPL
860 870 880 890 900
SLEVLNAHGS DSEKLPMHYK FNNFFDLSQQ VVNDFLLKTL TYVKHIPRGY
910 920 930 940 950
TQELINKAPT FSFSAPSHTN QNATFDLQSG SSVMTRLNHS WRNTLVGFGM
960 970 980 990 1000
LVEVAFPEDY CDATDVGISC VCRWSNKEGR SCRIERKFHC WAPWQVVPKV
1010 1020 1030 1040 1050
RKDHTFVFSD VNMRPSTGEG NDPDIWAGLV VFEFFPINQQ TKCLNDRFTV
1060 1070 1080 1090 1100
RRCGVRVINV ATGNTSLENI ALVLSLDPVE VSGYEVLRVS YDDLQEMDKV
1110 1120 1130 1140 1150
LFLYIASLFN DEDVDFVAPL IAGIDLDVSS GLKVLADVSL ISVSSNGEIV
1160 1170 1180 1190 1200
MHSLQRQMGK EILHGQSMLL SDCESSMTEN LSDVPKKKKK HSESRVKKVV
1210 1220 1230 1240 1250
SIPAIDEGDL WTWRKYGQKD ILGSRFPRGY YRCAYKFTHG CKATKQVQRS
1260 1270 1280
ETDSNMLAIT YLSEHNHPRP TKRKALADST RSTSSSIC
Length:1,288
Mass (Da):145,879
Last modified:November 26, 2014 - v1
Checksum:iB364DE7CE4DB6706
GO
Isoform 2 (identifier: P0DKH5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1188-1288: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:1,187
Mass (Da):134,276
Checksum:i7EF6A2240C692B11
GO

Sequence cautioni

The sequence BAB10247 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g45260 and At5g45270.Curated
The sequence BAB10248 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g45260 and At5g45270.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti395 – 3951L → S in BAC41834 (PubMed:11910074).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1188 – 1288101Missing in isoform 2. 1 PublicationVSP_015696Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AX103684 Genomic DNA. No translation available.
AB020744 Genomic DNA. Translation: BAB10247.1. Sequence problems.
AB020744 Genomic DNA. Translation: BAB10248.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95222.1.
CP002688 Genomic DNA. Translation: AED95223.1.
AK117156 mRNA. Translation: BAC41834.1.
AK226456 mRNA. Translation: BAE98598.1.
RefSeqiNP_001078715.1. NM_001085246.1. [P0DKH5-2]
NP_199339.2. NM_123894.3. [P0DKH5-1]
UniGeneiAt.30021.

Genome annotation databases

EnsemblPlantsiAT5G45260.1; AT5G45260.1; AT5G45260. [P0DKH5-1]
GeneIDi834562.
KEGGiath:AT5G45260.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

NIB-LRRS

Functional and comparative genomics of disease resistance gene homologs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AX103684 Genomic DNA. No translation available.
AB020744 Genomic DNA. Translation: BAB10247.1. Sequence problems.
AB020744 Genomic DNA. Translation: BAB10248.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED95222.1.
CP002688 Genomic DNA. Translation: AED95223.1.
AK117156 mRNA. Translation: BAC41834.1.
AK226456 mRNA. Translation: BAE98598.1.
RefSeqiNP_001078715.1. NM_001085246.1. [P0DKH5-2]
NP_199339.2. NM_123894.3. [P0DKH5-1]
UniGeneiAt.30021.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4C6SX-ray1.75A7-153[»]
4C6TX-ray2.65A/C6-153[»]
ProteinModelPortaliP0DKH5.
SMRiP0DKH5. Positions 8-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19811. 1 interaction.
DIPiDIP-61676N.
MINTiMINT-267462.
STRINGi3702.AT5G45260.1.

Proteomic databases

PaxDbiP0DKH5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G45260.1; AT5G45260.1; AT5G45260. [P0DKH5-1]
GeneIDi834562.
KEGGiath:AT5G45260.

Organism-specific databases

TAIRiAT5G45260.

Phylogenomic databases

eggNOGiENOG410JQXQ. Eukaryota.
ENOG410ZRAD. LUCA.
InParanoidiQ9FH83.
KOiK16225.
OMAiGNDATIM.
OrthoDBiEOG093611RA.
PhylomeDBiP0DKH5.

Miscellaneous databases

PROiP0DKH5.

Gene expression databases

GenevisibleiP0DKH5. AT.

Family and domain databases

Gene3Di2.20.25.80. 1 hit.
3.40.50.300. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR011713. Leu-rich_rpt_3.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR000157. TIR_dom.
IPR011991. WHTH_DNA-bd_dom.
IPR003657. WRKY_dom.
[Graphical view]
PfamiPF07725. LRR_3. 1 hit.
PF00931. NB-ARC. 1 hit.
PF03106. WRKY. 1 hit.
[Graphical view]
SMARTiSM00774. WRKY. 1 hit.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 1 hit.
SSF46785. SSF46785. 1 hit.
SSF52058. SSF52058. 1 hit.
SSF52200. SSF52200. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50811. WRKY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWR52C_ARATH
AccessioniPrimary (citable) accession number: P0DKH5
Secondary accession number(s): Q0WWA0
, Q689Y9, Q8GZ83, Q9FH83, Q9FH84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 26, 2014
Last sequence update: November 26, 2014
Last modified: September 7, 2016
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Ecotypes susceptible to C.higginsianum or R.solanacearum, such as cv. Columbia, contain a protein with a premature stop codon, while the longer allele found in cv. Nd-1, cv. Wassilewskija or cv. RLD confers resistance.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.