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Protein

Carboxyethyl-arginine beta-lactam-synthase

Gene

bls

Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-N2-(2-carboxyethyl)arginine = AMP + diphosphate + deoxyamidinoproclavaminate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: clavulanate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes clavulanate from D-glyceraldehyde 3-phosphate and L-arginine.
Proteins known to be involved in the 8 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Carboxyethyl-arginine beta-lactam-synthase (bls)
  3. Clavaminate synthase 2 (cs2), Clavaminate synthase 1 (cs1)
  4. Proclavaminate amidinohydrolase (pah)
  5. Clavaminate synthase 2 (cs2), Clavaminate synthase 1 (cs1)
  6. Clavaminate synthase 2 (cs2), Clavaminate synthase 1 (cs1)
  7. no protein annotated in this organism
  8. no protein annotated in this organism
This subpathway is part of the pathway clavulanate biosynthesis, which is itself part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes clavulanate from D-glyceraldehyde 3-phosphate and L-arginine, the pathway clavulanate biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi253MagnesiumBy similarity1
Metal bindingi351MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00112; UER00243.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxyethyl-arginine beta-lactam-synthase (EC:6.3.3.4)
Alternative name(s):
Beta-lactam synthetase
Gene namesi
Name:bls
OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Taxonomic identifieri443255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004190881 – 513Carboxyethyl-arginine beta-lactam-synthaseAdd BLAST513

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0DJQ8.
SMRiP0DJQ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the asparagine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105TCH. Bacteria.
COG0367. LUCA.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00733. Asn_synthase. 2 hits.
PF13537. GATase_7. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.

Sequencei

Sequence statusi: Complete.

P0DJQ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAPVLPAAF GFLASARTGG GRAPGPVFAT RGSHTDIDTP QGERSLAATL
60 70 80 90 100
VHAPSVAPDR AVARSLTGAP TTAVLAGEIY NRDELLSVLP AGPAPEGDAE
110 120 130 140 150
LVLRLLERYD LHAFRLVNGR FATVVRTGDR VLLATDHAGS VPLYTCVAPG
160 170 180 190 200
EVRASTEAKA LAAHRDPKGF PLADARRVAG LTGVYQVPAG AVMDIDLGSG
210 220 230 240 250
TAVTHRTWTP GLSRRILPEG EAVAAVRAAL EKAVAQRVTP GDTPLVVLSG
260 270 280 290 300
GIDSSGVAAC AHRAAGELDT VSMGTDTSNE FREARAVVDH LRTRHREITI
310 320 330 340 350
PTTELLAQLP YAVWASESVD PDIIEYLLPL TALYRALDGP ERRILTGYGA
360 370 380 390 400
DIPLGGMHRE DRLPALDTVL AHDMATFDGL NEMSPVLSTL AGHWTTHPYW
410 420 430 440 450
DREVLDLLVS LEAGLKRRHG RDKWVLRAAM ADALPAETVN RPKLGVHEGS
460 470 480 490 500
GTTSSFSRLL LDHGVAEDRV HEAKRQVVRE LFDLTVGGGR HPSEVDTDDV
510
VRSVADRTAR GAA
Length:513
Mass (Da):54,530
Last modified:September 5, 2012 - v1
Checksum:iEC2F460A77EB65CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87786 Genomic DNA. Translation: AAF86620.1.
RefSeqiWP_003952510.1. NZ_DS570624.1.

Similar proteinsi

Entry informationi

Entry nameiBLS_STRC2
AccessioniPrimary (citable) accession number: P0DJQ8
Secondary accession number(s): Q53938, Q9R8E3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: September 5, 2012
Last modified: July 5, 2017
This is version 26 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families