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Protein

Internalin-A

Gene

inlA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the entry of L.monocytogenes into cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Internalin-A
Gene namesi
Name:inlA
Ordered Locus Names:lmo0433
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence analysisAdd
BLAST
Chaini36 – 770735Internalin-APRO_0000005609Add
BLAST
Propeptidei771 – 80030Removed by sortase APRO_0000005610Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei770 – 7701Pentaglycyl murein peptidoglycan amidated threonine

Post-translational modificationi

Proteolytically cleaved between Thr-770 and Gly-771 by sortase A (SrtA).2 Publications

Keywords - PTMi

Peptidoglycan-anchor

Proteomic databases

PaxDbiP0DJM0.

Interactioni

Protein-protein interaction databases

IntActiP0DJM0. 1 interaction.
STRINGi169963.lmo0433.

Structurei

Secondary structure

1
800
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi44 – 463Combined sources
Helixi51 – 6010Combined sources
Beta strandi68 – 703Combined sources
Helixi72 – 765Combined sources
Beta strandi80 – 823Combined sources
Helixi94 – 963Combined sources
Beta strandi102 – 1043Combined sources
Helixi114 – 1163Combined sources
Beta strandi124 – 1263Combined sources
Helixi136 – 1383Combined sources
Beta strandi146 – 1483Combined sources
Helixi158 – 1603Combined sources
Beta strandi167 – 17610Combined sources
Helixi180 – 1823Combined sources
Beta strandi189 – 1968Combined sources
Helixi201 – 2033Combined sources
Beta strandi211 – 2133Combined sources
Helixi223 – 2275Combined sources
Beta strandi232 – 2354Combined sources
Helixi245 – 2495Combined sources
Beta strandi255 – 2573Combined sources
Helixi267 – 2715Combined sources
Beta strandi276 – 2794Combined sources
Helixi289 – 2913Combined sources
Beta strandi298 – 3014Combined sources
Helixi311 – 3133Combined sources
Beta strandi320 – 3234Combined sources
Helixi333 – 3375Combined sources
Beta strandi342 – 3454Combined sources
Helixi355 – 3595Combined sources
Beta strandi365 – 3673Combined sources
Helixi377 – 3793Combined sources
Beta strandi387 – 3893Combined sources
Helixi399 – 4013Combined sources
Beta strandi409 – 4113Combined sources
Beta strandi415 – 4184Combined sources
Beta strandi426 – 4316Combined sources
Beta strandi445 – 4484Combined sources
Beta strandi452 – 4543Combined sources
Beta strandi457 – 4615Combined sources
Beta strandi467 – 47913Combined sources
Beta strandi482 – 49413Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1O6SX-ray1.80A36-496[»]
1O6TX-ray1.60A36-496[»]
1O6VX-ray1.50A/B36-496[»]
2OMTX-ray2.00A36-496[»]
2OMUX-ray1.80A36-496[»]
2OMVX-ray1.90A36-495[»]
2OMWX-ray1.85A36-496[»]
2OMXX-ray1.70A36-496[»]
2OMYX-ray1.70A36-495[»]
2OMZX-ray1.60A36-495[»]
ProteinModelPortaliP0DJM0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 7641LRRNTAdd
BLAST
Repeati77 – 9822LRR 1Add
BLAST
Repeati99 – 12022LRR 2Add
BLAST
Repeati121 – 14222LRR 3Add
BLAST
Repeati143 – 16422LRR 4Add
BLAST
Repeati165 – 18622LRR 5Add
BLAST
Repeati187 – 20721LRR 6Add
BLAST
Repeati208 – 22922LRR 7Add
BLAST
Repeati230 – 25122LRR 8Add
BLAST
Repeati252 – 27322LRR 9Add
BLAST
Repeati274 – 29522LRR 10Add
BLAST
Repeati296 – 31722LRR 11Add
BLAST
Repeati318 – 33922LRR 12Add
BLAST
Repeati340 – 36122LRR 13Add
BLAST
Repeati362 – 38322LRR 14Add
BLAST
Repeati384 – 40522LRR 15Add
BLAST
Domaini416 – 50590LRRCTAdd
BLAST
Repeati518 – 58770B-1Add
BLAST
Repeati588 – 65770B-2Add
BLAST
Repeati658 – 70649B-3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni518 – 7061893 X approximate tandem repeats, type BAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi767 – 7715LPXTG sorting signal

Sequence similaritiesi

Contains 15 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiENOG4108NJY. Bacteria.
COG4886. LUCA.
KOiK13730.
OMAiFGSGVWI.
PhylomeDBiP0DJM0.

Family and domain databases

Gene3Di2.60.40.1220. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR014755. Cu-Rt/internalin_Ig-like.
IPR019948. Gram-positive_anchor.
IPR014756. Ig_E-set.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013378. Listeria/Bacterioides_rpt.
IPR012569. LRR-contain_adjacent_dom.
[Graphical view]
PfamiPF09479. Flg_new. 3 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF12799. LRR_4. 4 hits.
PF08191. LRR_adjacent. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 12 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02543. List_Bact_rpt. 3 hits.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS51450. LRR. 14 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0DJM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKKRYVWLK SILVAILVFG SGVWINTSNG TNAQAATITQ DTPINQIFTD
60 70 80 90 100
TALAEKMKTV LGKTNVTDTV SQTDLDQVTT LQADRLGIKS IDGVEYLNNL
110 120 130 140 150
TQINFSNNQL TDITPLKNLT KLVDILMNNN QIADITPLAN LTNLTGLTLF
160 170 180 190 200
NNQITDIDPL KNLTNLNRLE LSSNTISDIS ALSGLTSLQQ LSFGNQVTDL
210 220 230 240 250
KPLANLTTLE RLDISSNKVS DISVLAKLTN LESLIATNNQ ISDITPLGIL
260 270 280 290 300
TNLDELSLNG NQLKDIGTLA SLTNLTDLDL ANNQISNLAP LSGLTKLTEL
310 320 330 340 350
KLGANQISNI SPLAGLTALT NLELNENQLE DISPISNLKN LTYLTLYFNN
360 370 380 390 400
ISDISPVSSL TKLQRLFFYN NKVSDVSSLA NLTNINWLSA GHNQISDLTP
410 420 430 440 450
LANLTRITQL GLNDQAWTNA PVNYKANVSI PNTVKNVTGA LIAPATISDG
460 470 480 490 500
GSYTEPDITW NLPSYTNEVS YTFSQPVTIG KGTTTFSGTV TQPLKAIFNV
510 520 530 540 550
KFHVDGKETT KEVEAGNLLT EPAKPVKEGH TFVGWFDAQT GGTKWNFSTD
560 570 580 590 600
KMPTNDINLY AQFSINSYTA TFDNDGVTTS QTVDYQGLLQ EPTAPTKEGY
610 620 630 640 650
TFKGWYDAKT GGDKWDFATS KMPAKNITLY AQYSANSYTA TFDVDGKSTT
660 670 680 690 700
QAVDYQGLLK EPKAPTKAGY TFKGWYDEKT DGKKWDFATD KMPANDITLY
710 720 730 740 750
AQFTKNPVAP PTTGGNTPPT TNNGGNTTPP SANIPGSDTS NTSTGNSAST
760 770 780 790 800
TSTMNAYDPY NSKEASLPTT GDSDNALYLL LGLLAVGTAM ALTKKARASK
Length:800
Mass (Da):86,493
Last modified:September 5, 2012 - v1
Checksum:i4D1B03A5CF7CCDE3
GO

Sequence cautioni

The sequence AAA25289 differs from that shown. Reason: Frameshift at position 56. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti3 – 31K → R in strain: H4.
Natural varianti19 – 191F → L in strain: H4.
Natural varianti51 – 511T → A in strain: EGD-SmR / Serovar 1/2a.
Natural varianti94 – 941V → L in strain: EGD-SmR / Serovar 1/2a, F4233 / Serotype 1/2b, F6789 / Serotype 1/2b, F5782 / Serotype 4b and H4.
Natural varianti118 – 1181N → D in strain: EGD-SmR / Serovar 1/2a, F4233 / Serotype 1/2b, F6789 / Serotype 1/2b, F5782 / Serotype 4b and H4.
Natural varianti142 – 1421T → S in strain: F5782 / Serotype 4b and H4.
Natural varianti187 – 1871S → N in strain: EGD-SmR / Serovar 1/2a, F4233 / Serotype 1/2b, F6789 / Serotype 1/2b and H4.
Natural varianti193 – 1931F → L in strain: F5782 / Serotype 4b, F4233 / Serotype 1/2b and F6789 / Serotype 1/2b.
Natural varianti253 – 2531L → W in strain: F4233 / Serotype 1/2b and F6789 / Serotype 1/2b.
Natural varianti292 – 2921S → P in strain: F4233 / Serotype 1/2b and F5782 / Serotype 4b.
Natural varianti292 – 2921S → R in strain: F6789 / Serotype 1/2b.
Natural varianti321 – 3211N → S in strain: H4.
Natural varianti381 – 3811N → S in strain: H4.
Natural varianti416 – 4161A → E in strain: F4233 / Serotype 1/2b, F6789 / Serotype 1/2b and F5782 / Serotype 4b.
Natural varianti454 – 4541T → A in strain: EGD-SmR / Serovar 1/2a and H4.
Natural varianti474 – 4741S → N in strain: H4.
Natural varianti476 – 4761P → S in strain: H4.
Natural varianti500 – 5001V → A in strain: EGD-SmR / Serovar 1/2a and H4.
Natural varianti530 – 5301H → Y in strain: H4.
Natural varianti558 – 5581N → D in strain: H4.
Natural varianti573 – 5731D → E in strain: EGD-SmR / Serovar 1/2a.
Natural varianti594 – 5941A → P in strain: EGD-SmR / Serovar 1/2a.
Natural varianti648 – 6481S → T in strain: H4.
Natural varianti664 – 6641A → T in strain: H4.
Natural varianti729 – 7291P → S in strain: H4.
Natural varianti738 – 7381D → N in strain: H4.
Natural varianti781 – 7811L → I in strain: H4.
Natural varianti790 – 7901M → V in strain: H4.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67471 Genomic DNA. Translation: AAA25289.1. Frameshift.
AJ012346 Genomic DNA. Translation: CAC20628.1.
DQ132794 Genomic DNA. Translation: AAZ53235.1.
AL591975 Genomic DNA. Translation: CAC98512.1.
U25448 Genomic DNA. Translation: AAA69530.1.
U25451 Genomic DNA. Translation: AAA69533.1.
U25454 Genomic DNA. Translation: AAA69536.1.
PIRiAB1129.
RefSeqiNP_463962.1. NC_003210.1.
WP_010989462.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAC98512; CAC98512; CAC98512.
GeneIDi985151.
KEGGilmo:lmo0433.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67471 Genomic DNA. Translation: AAA25289.1. Frameshift.
AJ012346 Genomic DNA. Translation: CAC20628.1.
DQ132794 Genomic DNA. Translation: AAZ53235.1.
AL591975 Genomic DNA. Translation: CAC98512.1.
U25448 Genomic DNA. Translation: AAA69530.1.
U25451 Genomic DNA. Translation: AAA69533.1.
U25454 Genomic DNA. Translation: AAA69536.1.
PIRiAB1129.
RefSeqiNP_463962.1. NC_003210.1.
WP_010989462.1. NC_003210.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1O6SX-ray1.80A36-496[»]
1O6TX-ray1.60A36-496[»]
1O6VX-ray1.50A/B36-496[»]
2OMTX-ray2.00A36-496[»]
2OMUX-ray1.80A36-496[»]
2OMVX-ray1.90A36-495[»]
2OMWX-ray1.85A36-496[»]
2OMXX-ray1.70A36-496[»]
2OMYX-ray1.70A36-495[»]
2OMZX-ray1.60A36-495[»]
ProteinModelPortaliP0DJM0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0DJM0. 1 interaction.
STRINGi169963.lmo0433.

Proteomic databases

PaxDbiP0DJM0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC98512; CAC98512; CAC98512.
GeneIDi985151.
KEGGilmo:lmo0433.

Phylogenomic databases

eggNOGiENOG4108NJY. Bacteria.
COG4886. LUCA.
KOiK13730.
OMAiFGSGVWI.
PhylomeDBiP0DJM0.

Family and domain databases

Gene3Di2.60.40.1220. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR014755. Cu-Rt/internalin_Ig-like.
IPR019948. Gram-positive_anchor.
IPR014756. Ig_E-set.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013378. Listeria/Bacterioides_rpt.
IPR012569. LRR-contain_adjacent_dom.
[Graphical view]
PfamiPF09479. Flg_new. 3 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF12799. LRR_4. 4 hits.
PF08191. LRR_adjacent. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 12 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02543. List_Bact_rpt. 3 hits.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS51450. LRR. 14 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiINLA_LISMO
AccessioniPrimary (citable) accession number: P0DJM0
Secondary accession number(s): P25146
, Q45GD5, Q45GD6, Q48748, Q48749, Q48750, Q48752, Q9EXG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: September 5, 2012
Last modified: September 7, 2016
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The formation of the complex between internalin and human host receptor (CDH1) is calcium-dependent.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.