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Protein

Internalin-A

Gene

inlA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the entry of L.monocytogenes into cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Internalin-A
Gene namesi
Name:inlA
Ordered Locus Names:lmo0433
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000000560936 – 770Internalin-AAdd BLAST735
PropeptideiPRO_0000005610771 – 800Removed by sortase AAdd BLAST30

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei770Pentaglycyl murein peptidoglycan amidated threonine1

Post-translational modificationi

Proteolytically cleaved between Thr-770 and Gly-771 by sortase A (SrtA).2 Publications

Keywords - PTMi

Peptidoglycan-anchor

Proteomic databases

PaxDbiP0DJM0.

Interactioni

Protein-protein interaction databases

IntActiP0DJM0. 1 interactor.
STRINGi169963.lmo0433.

Structurei

Secondary structure

1800
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi44 – 46Combined sources3
Helixi51 – 60Combined sources10
Beta strandi68 – 70Combined sources3
Helixi72 – 76Combined sources5
Beta strandi80 – 82Combined sources3
Helixi94 – 96Combined sources3
Beta strandi102 – 104Combined sources3
Helixi114 – 116Combined sources3
Beta strandi124 – 126Combined sources3
Helixi136 – 138Combined sources3
Beta strandi146 – 148Combined sources3
Helixi158 – 160Combined sources3
Beta strandi167 – 176Combined sources10
Helixi180 – 182Combined sources3
Beta strandi189 – 196Combined sources8
Helixi201 – 203Combined sources3
Beta strandi211 – 213Combined sources3
Helixi223 – 227Combined sources5
Beta strandi232 – 235Combined sources4
Helixi245 – 249Combined sources5
Beta strandi255 – 257Combined sources3
Helixi267 – 271Combined sources5
Beta strandi276 – 279Combined sources4
Helixi289 – 291Combined sources3
Beta strandi298 – 301Combined sources4
Helixi311 – 313Combined sources3
Beta strandi320 – 323Combined sources4
Helixi333 – 337Combined sources5
Beta strandi342 – 345Combined sources4
Helixi355 – 359Combined sources5
Beta strandi365 – 367Combined sources3
Helixi377 – 379Combined sources3
Beta strandi387 – 389Combined sources3
Helixi399 – 401Combined sources3
Beta strandi409 – 411Combined sources3
Beta strandi415 – 418Combined sources4
Beta strandi426 – 431Combined sources6
Beta strandi445 – 448Combined sources4
Beta strandi452 – 454Combined sources3
Beta strandi457 – 461Combined sources5
Beta strandi467 – 479Combined sources13
Beta strandi482 – 494Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1O6SX-ray1.80A36-496[»]
1O6TX-ray1.60A36-496[»]
1O6VX-ray1.50A/B36-496[»]
2OMTX-ray2.00A36-496[»]
2OMUX-ray1.80A36-496[»]
2OMVX-ray1.90A36-495[»]
2OMWX-ray1.85A36-496[»]
2OMXX-ray1.70A36-496[»]
2OMYX-ray1.70A36-495[»]
2OMZX-ray1.60A36-495[»]
ProteinModelPortaliP0DJM0.
SMRiP0DJM0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 76LRRNTAdd BLAST41
Repeati77 – 98LRR 1Add BLAST22
Repeati99 – 120LRR 2Add BLAST22
Repeati121 – 142LRR 3Add BLAST22
Repeati143 – 164LRR 4Add BLAST22
Repeati165 – 186LRR 5Add BLAST22
Repeati187 – 207LRR 6Add BLAST21
Repeati208 – 229LRR 7Add BLAST22
Repeati230 – 251LRR 8Add BLAST22
Repeati252 – 273LRR 9Add BLAST22
Repeati274 – 295LRR 10Add BLAST22
Repeati296 – 317LRR 11Add BLAST22
Repeati318 – 339LRR 12Add BLAST22
Repeati340 – 361LRR 13Add BLAST22
Repeati362 – 383LRR 14Add BLAST22
Repeati384 – 405LRR 15Add BLAST22
Domaini416 – 505LRRCTAdd BLAST90
Repeati518 – 587B-1Add BLAST70
Repeati588 – 657B-2Add BLAST70
Repeati658 – 706B-3Add BLAST49

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni518 – 7063 X approximate tandem repeats, type BAdd BLAST189

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi767 – 771LPXTG sorting signal5

Sequence similaritiesi

Contains 15 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal

Phylogenomic databases

eggNOGiENOG4108NJY. Bacteria.
COG4886. LUCA.
KOiK13730.
OMAiFGSGVWI.
PhylomeDBiP0DJM0.

Family and domain databases

Gene3Di2.60.40.1220. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR014755. Cu-Rt/internalin_Ig-like.
IPR019948. Gram-positive_anchor.
IPR014756. Ig_E-set.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013378. Listeria/Bacterioides_rpt.
IPR012569. LRR-contain_adjacent_dom.
[Graphical view]
PfamiPF09479. Flg_new. 3 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF12799. LRR_4. 4 hits.
PF08191. LRR_adjacent. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 12 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02543. List_Bact_rpt. 3 hits.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS51450. LRR. 14 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0DJM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKKRYVWLK SILVAILVFG SGVWINTSNG TNAQAATITQ DTPINQIFTD
60 70 80 90 100
TALAEKMKTV LGKTNVTDTV SQTDLDQVTT LQADRLGIKS IDGVEYLNNL
110 120 130 140 150
TQINFSNNQL TDITPLKNLT KLVDILMNNN QIADITPLAN LTNLTGLTLF
160 170 180 190 200
NNQITDIDPL KNLTNLNRLE LSSNTISDIS ALSGLTSLQQ LSFGNQVTDL
210 220 230 240 250
KPLANLTTLE RLDISSNKVS DISVLAKLTN LESLIATNNQ ISDITPLGIL
260 270 280 290 300
TNLDELSLNG NQLKDIGTLA SLTNLTDLDL ANNQISNLAP LSGLTKLTEL
310 320 330 340 350
KLGANQISNI SPLAGLTALT NLELNENQLE DISPISNLKN LTYLTLYFNN
360 370 380 390 400
ISDISPVSSL TKLQRLFFYN NKVSDVSSLA NLTNINWLSA GHNQISDLTP
410 420 430 440 450
LANLTRITQL GLNDQAWTNA PVNYKANVSI PNTVKNVTGA LIAPATISDG
460 470 480 490 500
GSYTEPDITW NLPSYTNEVS YTFSQPVTIG KGTTTFSGTV TQPLKAIFNV
510 520 530 540 550
KFHVDGKETT KEVEAGNLLT EPAKPVKEGH TFVGWFDAQT GGTKWNFSTD
560 570 580 590 600
KMPTNDINLY AQFSINSYTA TFDNDGVTTS QTVDYQGLLQ EPTAPTKEGY
610 620 630 640 650
TFKGWYDAKT GGDKWDFATS KMPAKNITLY AQYSANSYTA TFDVDGKSTT
660 670 680 690 700
QAVDYQGLLK EPKAPTKAGY TFKGWYDEKT DGKKWDFATD KMPANDITLY
710 720 730 740 750
AQFTKNPVAP PTTGGNTPPT TNNGGNTTPP SANIPGSDTS NTSTGNSAST
760 770 780 790 800
TSTMNAYDPY NSKEASLPTT GDSDNALYLL LGLLAVGTAM ALTKKARASK
Length:800
Mass (Da):86,493
Last modified:September 5, 2012 - v1
Checksum:i4D1B03A5CF7CCDE3
GO

Sequence cautioni

The sequence AAA25289 differs from that shown. Reason: Frameshift at position 56.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti3K → R in strain: H4. 1
Natural varianti19F → L in strain: H4. 1
Natural varianti51T → A in strain: EGD-SmR / Serovar 1/2a. 1
Natural varianti94V → L in strain: EGD-SmR / Serovar 1/2a, F4233 / Serotype 1/2b, F6789 / Serotype 1/2b, F5782 / Serotype 4b and H4. 1
Natural varianti118N → D in strain: EGD-SmR / Serovar 1/2a, F4233 / Serotype 1/2b, F6789 / Serotype 1/2b, F5782 / Serotype 4b and H4. 1
Natural varianti142T → S in strain: F5782 / Serotype 4b and H4. 1
Natural varianti187S → N in strain: EGD-SmR / Serovar 1/2a, F4233 / Serotype 1/2b, F6789 / Serotype 1/2b and H4. 1
Natural varianti193F → L in strain: F5782 / Serotype 4b, F4233 / Serotype 1/2b and F6789 / Serotype 1/2b. 1
Natural varianti253L → W in strain: F4233 / Serotype 1/2b and F6789 / Serotype 1/2b. 1
Natural varianti292S → P in strain: F4233 / Serotype 1/2b and F5782 / Serotype 4b. 1
Natural varianti292S → R in strain: F6789 / Serotype 1/2b. 1
Natural varianti321N → S in strain: H4. 1
Natural varianti381N → S in strain: H4. 1
Natural varianti416A → E in strain: F4233 / Serotype 1/2b, F6789 / Serotype 1/2b and F5782 / Serotype 4b. 1
Natural varianti454T → A in strain: EGD-SmR / Serovar 1/2a and H4. 1
Natural varianti474S → N in strain: H4. 1
Natural varianti476P → S in strain: H4. 1
Natural varianti500V → A in strain: EGD-SmR / Serovar 1/2a and H4. 1
Natural varianti530H → Y in strain: H4. 1
Natural varianti558N → D in strain: H4. 1
Natural varianti573D → E in strain: EGD-SmR / Serovar 1/2a. 1
Natural varianti594A → P in strain: EGD-SmR / Serovar 1/2a. 1
Natural varianti648S → T in strain: H4. 1
Natural varianti664A → T in strain: H4. 1
Natural varianti729P → S in strain: H4. 1
Natural varianti738D → N in strain: H4. 1
Natural varianti781L → I in strain: H4. 1
Natural varianti790M → V in strain: H4. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67471 Genomic DNA. Translation: AAA25289.1. Frameshift.
AJ012346 Genomic DNA. Translation: CAC20628.1.
DQ132794 Genomic DNA. Translation: AAZ53235.1.
AL591975 Genomic DNA. Translation: CAC98512.1.
U25448 Genomic DNA. Translation: AAA69530.1.
U25451 Genomic DNA. Translation: AAA69533.1.
U25454 Genomic DNA. Translation: AAA69536.1.
PIRiAB1129.
RefSeqiNP_463962.1. NC_003210.1.
WP_010989462.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAC98512; CAC98512; CAC98512.
GeneIDi985151.
KEGGilmo:lmo0433.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67471 Genomic DNA. Translation: AAA25289.1. Frameshift.
AJ012346 Genomic DNA. Translation: CAC20628.1.
DQ132794 Genomic DNA. Translation: AAZ53235.1.
AL591975 Genomic DNA. Translation: CAC98512.1.
U25448 Genomic DNA. Translation: AAA69530.1.
U25451 Genomic DNA. Translation: AAA69533.1.
U25454 Genomic DNA. Translation: AAA69536.1.
PIRiAB1129.
RefSeqiNP_463962.1. NC_003210.1.
WP_010989462.1. NC_003210.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1O6SX-ray1.80A36-496[»]
1O6TX-ray1.60A36-496[»]
1O6VX-ray1.50A/B36-496[»]
2OMTX-ray2.00A36-496[»]
2OMUX-ray1.80A36-496[»]
2OMVX-ray1.90A36-495[»]
2OMWX-ray1.85A36-496[»]
2OMXX-ray1.70A36-496[»]
2OMYX-ray1.70A36-495[»]
2OMZX-ray1.60A36-495[»]
ProteinModelPortaliP0DJM0.
SMRiP0DJM0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0DJM0. 1 interactor.
STRINGi169963.lmo0433.

Proteomic databases

PaxDbiP0DJM0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC98512; CAC98512; CAC98512.
GeneIDi985151.
KEGGilmo:lmo0433.

Phylogenomic databases

eggNOGiENOG4108NJY. Bacteria.
COG4886. LUCA.
KOiK13730.
OMAiFGSGVWI.
PhylomeDBiP0DJM0.

Family and domain databases

Gene3Di2.60.40.1220. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR014755. Cu-Rt/internalin_Ig-like.
IPR019948. Gram-positive_anchor.
IPR014756. Ig_E-set.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013378. Listeria/Bacterioides_rpt.
IPR012569. LRR-contain_adjacent_dom.
[Graphical view]
PfamiPF09479. Flg_new. 3 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF12799. LRR_4. 4 hits.
PF08191. LRR_adjacent. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 12 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02543. List_Bact_rpt. 3 hits.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS51450. LRR. 14 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiINLA_LISMO
AccessioniPrimary (citable) accession number: P0DJM0
Secondary accession number(s): P25146
, Q45GD5, Q45GD6, Q48748, Q48749, Q48750, Q48752, Q9EXG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: September 5, 2012
Last modified: November 2, 2016
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The formation of the complex between internalin and human host receptor (CDH1) is calcium-dependent.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.