Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0DJK0

- SRTX1_ATRIR

UniProt

P0DJK0 - SRTX1_ATRIR

Protein

Sarafotoxin-i1

Gene
N/A
Organism
Atractaspis irregularis (Variable burrowing asp) (Elaps irregularis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm By similarity.By similarity
    Sarafotoxin-i3: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm By similarity. Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range).By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei105 – 1051Endothelin-receptor binding siteBy similarity

    GO - Biological processi

    1. regulation of vasoconstriction Source: InterPro
    2. vasoconstriction Source: UniProtKB-KW

    Keywords - Molecular functioni

    Cardiotoxin, G-protein coupled receptor impairing toxin, Toxin, Vasoactive, Vasoconstrictor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sarafotoxin-i1
    Short name:
    SRTX-i1
    Alternative name(s):
    Sarafotoxin-i3
    Short name:
    SRTX-i3
    OrganismiAtractaspis irregularis (Variable burrowing asp) (Elaps irregularis)
    Taxonomic identifieri512568 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaAtractaspididaeAtractaspis

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi106 – 1094Missing: Drastic 4-orders or magnitude increase in affinity for ET-B receptors. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Propeptidei24 – 84611 PublicationPRO_0000421164Add
    BLAST
    Peptidei85 – 10925Sarafotoxin-i1PRO_0000421165Add
    BLAST
    Propeptidei112 – 1187PRO_0000421166

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi85 ↔ 99By similarity
    Disulfide bondi87 ↔ 95By similarity

    Post-translational modificationi

    Different length molecules ranging from 15 (85-99) to 30 amino acids (85-114) have been found in the venom.1 Publication

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LDENMR-A85-109[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the endothelin/sarafotoxin family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR019764. Endothelin_toxin_CS.
    IPR001928. Endothln-like_toxin.
    [Graphical view]
    PfamiPF00322. Endothelin. 1 hit.
    [Graphical view]
    PROSITEiPS00270. ENDOTHELIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0DJK0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALLPRLAAG GLLLLLALAA LDGKPAPPKL LQKLMDGGQR RSEDQAAAGR    50
    IIDYEDGDEP VAVSVGDTKQ AARALSPLRK PQPLCSCTDM SDLECMNFCH 100
    KDVIWINRNR KPSPIQSS 118
    Length:118
    Mass (Da):12,722
    Last modified:February 6, 2013 - v1
    Checksum:iD16BD000685310C1
    GO

    RNA editingi

    RNA editing may explain why no precursor for this sequence have been cloned.Curated

    Mass spectrometryi

    Molecular mass is 2972.2 Da from positions 85 - 109. Determined by ESI. Monoisotopic mass, sarafotoxin-i1.1 Publication
    Molecular mass is 2958.2 Da from positions 85 - 109. Determined by ESI. Monoisotopic mass, sarafotoxin-i3.1 Publication

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti106 – 1061I → V in RNA edited version, Sarafotoxin-i3.

    Keywords - Coding sequence diversityi

    RNA editing

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LDE NMR - A 85-109 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019764. Endothelin_toxin_CS.
    IPR001928. Endothln-like_toxin.
    [Graphical view ]
    Pfami PF00322. Endothelin. 1 hit.
    [Graphical view ]
    PROSITEi PS00270. ENDOTHELIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of toxins within crude venoms by combined use of Fourier transform mass spectrometry and cloning."
      Quinton L., Le Caer J.P., Phan G., Ligny-Lemaire C., Bourdais-Jomaron J., Ducancel F., Chamot-Rooke J.
      Anal. Chem. 77:6630-6639(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 85-109, MASS SPECTROMETRY, PROBABLE RNA EDITING.
      Tissue: Venom and Venom gland.
    2. "Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: role of the C-terminus extension."
      Mourier G., Hajj M., Cordier F., Zorba A., Gao X., Coskun T., Herbet A., Marcon E., Beau F., Delepierre M., Ducancel F., Servent D.
      Biochimie 94:461-470(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SYNTHESIS OF 85-109, FUNCTION (SARAFOTOXIN-I3), STRUCTURE BY NMR OF 85-109, DISULFIDE BONDS, MUTAGENESIS OF 106-ILE--ASN-109.

    Entry informationi

    Entry nameiSRTX1_ATRIR
    AccessioniPrimary (citable) accession number: P0DJK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2013
    Last sequence update: February 6, 2013
    Last modified: October 1, 2014
    This is version 9 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3