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P0DJK0

- SRTX1_ATRIR

UniProt

P0DJK0 - SRTX1_ATRIR

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Protein

Sarafotoxin-i1

Gene
N/A
Organism
Atractaspis irregularis (Variable burrowing asp) (Elaps irregularis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm (By similarity).By similarity
Sarafotoxin-i3: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei105 – 1051Endothelin-receptor binding siteBy similarity

GO - Biological processi

  1. regulation of vasoconstriction Source: InterPro
  2. vasoconstriction Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Cardiotoxin, G-protein coupled receptor impairing toxin, Toxin, Vasoactive, Vasoconstrictor

Names & Taxonomyi

Protein namesi
Recommended name:
Sarafotoxin-i1
Short name:
SRTX-i1
Alternative name(s):
Sarafotoxin-i3
Short name:
SRTX-i3
OrganismiAtractaspis irregularis (Variable burrowing asp) (Elaps irregularis)
Taxonomic identifieri512568 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaLamprophiidaeAtractaspidinaeAtractaspis

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi106 – 1094Missing: Drastic 4-orders or magnitude increase in affinity for ET-B receptors. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Propeptidei24 – 84611 PublicationPRO_0000421164Add
BLAST
Peptidei85 – 10925Sarafotoxin-i1PRO_0000421165Add
BLAST
Propeptidei112 – 1187PRO_0000421166

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi85 ↔ 99By similarity
Disulfide bondi87 ↔ 95By similarity

Post-translational modificationi

Different length molecules ranging from 15 (85-99) to 30 amino acids (85-114) have been found in the venom.1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LDENMR-A85-109[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the endothelin/sarafotoxin family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view]
PfamiPF00322. Endothelin. 1 hit.
[Graphical view]
PROSITEiPS00270. ENDOTHELIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0DJK0 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALLPRLAAG GLLLLLALAA LDGKPAPPKL LQKLMDGGQR RSEDQAAAGR
60 70 80 90 100
IIDYEDGDEP VAVSVGDTKQ AARALSPLRK PQPLCSCTDM SDLECMNFCH
110
KDVIWINRNR KPSPIQSS
Length:118
Mass (Da):12,722
Last modified:February 6, 2013 - v1
Checksum:iD16BD000685310C1
GO

RNA editingi

RNA editing may explain why no precursor for this sequence have been cloned.Curated

Mass spectrometryi

Molecular mass is 2972.2 Da from positions 85 - 109. Determined by ESI. Monoisotopic mass, sarafotoxin-i1.1 Publication
Molecular mass is 2958.2 Da from positions 85 - 109. Determined by ESI. Monoisotopic mass, sarafotoxin-i3.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti106 – 1061I → V in RNA edited version, Sarafotoxin-i3.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LDE NMR - A 85-109 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR019764. Endothelin_toxin_CS.
IPR001928. Endothln-like_toxin.
[Graphical view ]
Pfami PF00322. Endothelin. 1 hit.
[Graphical view ]
PROSITEi PS00270. ENDOTHELIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of toxins within crude venoms by combined use of Fourier transform mass spectrometry and cloning."
    Quinton L., Le Caer J.P., Phan G., Ligny-Lemaire C., Bourdais-Jomaron J., Ducancel F., Chamot-Rooke J.
    Anal. Chem. 77:6630-6639(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 85-109, MASS SPECTROMETRY, PROBABLE RNA EDITING.
    Tissue: Venom and Venom gland.
  2. "Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: role of the C-terminus extension."
    Mourier G., Hajj M., Cordier F., Zorba A., Gao X., Coskun T., Herbet A., Marcon E., Beau F., Delepierre M., Ducancel F., Servent D.
    Biochimie 94:461-470(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SYNTHESIS OF 85-109, FUNCTION (SARAFOTOXIN-I3), STRUCTURE BY NMR OF 85-109, DISULFIDE BONDS, MUTAGENESIS OF 106-ILE--ASN-109.

Entry informationi

Entry nameiSRTX1_ATRIR
AccessioniPrimary (citable) accession number: P0DJK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: February 6, 2013
Last modified: October 29, 2014
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3