P0DJH6 (CENPW_CHICK) Reviewed, UniProtKB/Swiss-Prot
Last modified July 9, 2014. Version 16. History...
Names and origin
|Protein names||Recommended name:|
Centromere protein W
|Organism||Gallus gallus (Chicken) [Reference proteome]|
|Taxonomic identifier||9031 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Testudines + Archosauria group › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus|
|Sequence length||76 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation By similarity. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres By similarity. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis. Ref.2 Ref.4
Part of a centromere complex consisting of CENPA, CENPT and CENPW By similarity. Part of a centromere complex consisting of histone H3, CENPT and CENPW. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW. Interacts directly with CENPT. Binds DNA. Ref.2 Ref.3 Ref.4
Nucleus. Chromosome › centromere › kinetochore. Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore. Ref.2 Ref.4
Association with CENPA-containing complexes may be indirect and due to the proximity of centromeric nucleosomes containing histone H3 with those containing CENPA.
Belongs to the CENPW family.
|Biological process||Cell cycle|
|Gene Ontology (GO)|
Inferred from electronic annotation. Source: Ensemblmitotic nuclear division
Inferred from electronic annotation. Source: UniProtKB-KW
|Cellular_component||condensed chromosome kinetochore|
Inferred from electronic annotation. Source: UniProtKB-SubCellnucleus
Inferred from electronic annotation. Source: UniProtKB-SubCell
Inferred from electronic annotation. Source: UniProtKB-KWprotein binding
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 76||76||Centromere protein W||PRO_0000417384|
|Mutagenesis||7||1||R → A: Lethal mitotic defects; when associated with A-22 and A-75. Lethal mitotic defects; when associated with A-11; A-12; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-11; A-12; A-22 and A-75. Ref.2 Ref.4|
|Mutagenesis||11||1||R → A: Lethal mitotic defects; when associated with A-7; A-12; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-12; A-22 and A-75. Ref.2 Ref.4|
|Mutagenesis||12||1||K → A: Lethal mitotic defects; when associated with A-7; A-11; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-11; A-22 and A-75. Ref.2 Ref.4|
|Mutagenesis||22||1||R → A: Lethal mitotic defects; when associated with A-7 and A-75. Lethal mitotic defects; when associated with A-7; A-11; A-12; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-11; A-12 and A-75. Ref.2 Ref.4|
|Mutagenesis||54||1||K → A: Lethal mitotic defects; when associated with A-7; A-11; A-12; A-22; and A-56. Ref.4|
|Mutagenesis||56||1||K → A: Lethal mitotic defects; when associated with A-7; A-11; A-12; A-22 and A-54. Ref.4|
|Mutagenesis||75||1||R → A: Lethal mitotic defects; when associated with A-7 and A-22. Lethal mitotic defects; when associated with A-7; A-11; A-12 and A-22. Ref.2|
Helix Strand Turn
|Helix||7 – 17||11|
|Helix||27 – 52||26|
|Beta strand||56 – 58||3|
|Helix||60 – 73||14|
|||"A comprehensive collection of chicken cDNAs."|
Boardman P.E., Sanz-Ezquerro J., Overton I.M., Burt D.W., Bosch E., Fong W.T., Tickle C., Brown W.R., Wilson S.A., Hubbard S.J.
Curr. Biol. 12:1965-1969(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: White Leghorn Hisex.
|||"CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore."|
Hori T., Amano M., Suzuki A., Backer C.B., Welburn J.P., Dong Y., McEwen B.F., Shang W.-H., Suzuki E., Okawa K., Cheeseman I.M., Fukagawa T.
Cell 135:1039-1052(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF ARG-7; ARG-11; LYS-12; ARG-22 AND ARG-75, SUBCELLULAR LOCATION.
|||"Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins."|
Suzuki A., Hori T., Nishino T., Usukura J., Miyagi A., Morikawa K., Fukagawa T.
J. Cell Biol. 193:125-140(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CENPT.
|||"CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold."|
Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T.
Cell 148:487-501(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-7; ARG-11; LYS-12; ARG-22; LYS-54 AND LYS-56.
|BU413473 mRNA. No translation available.|
|RefSeq||NP_001264662.1. NM_001277733.1. |
3D structure databases
Protein-protein interaction databases
|IntAct||P0DJH6. 3 interactions.|
Protocols and materials databases
Genome annotation databases
Family and domain databases
|Gene3D||188.8.131.52. 1 hit. |
|InterPro||IPR028847. CENP-W. |
|Pfam||PF15510. CENP-W. 1 hit. |
|SUPFAM||SSF47113. SSF47113. 1 hit. |
|Accession||Primary (citable) accession number: P0DJH6|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|