P0DJH6 (CENPW_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centromere protein W
Short name=CENP-W

Gene names

Name:

CENPW

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	76 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation By similarity. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres By similarity. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis. Ref.2 Ref.4
Subunit structure	Part of a centromere complex consisting of CENPA, CENPT and CENPW By similarity. Part of a centromere complex consisting of histone H3, CENPT and CENPW. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW. Interacts directly with CENPT. Binds DNA. Ref.2 Ref.3 Ref.4
Subcellular location	Nucleus. Chromosome › centromere › kinetochore. Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore. Ref.2 Ref.4
Miscellaneous	Association with CENPA-containing complexes may be indirect and due to the proximity of centromeric nucleosomes containing histone H3 with those containing CENPA.
Sequence similarities	Belongs to the CENPW family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Centromere Chromosome Kinetochore Nucleus
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CENPT	F1NPG5	4	EBI-2132287,EBI-2132248

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 76	76	Centromere protein W		PRO_0000417384
Experimental info
	Mutagenesis	7	1	R → A: Lethal mitotic defects; when associated with A-22 and A-75. Lethal mitotic defects; when associated with A-11; A-12; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-11; A-12; A-22 and A-75. Ref.2 Ref.4
	Mutagenesis	11	1	R → A: Lethal mitotic defects; when associated with A-7; A-12; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-12; A-22 and A-75. Ref.2 Ref.4
	Mutagenesis	12	1	K → A: Lethal mitotic defects; when associated with A-7; A-11; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-11; A-22 and A-75. Ref.2 Ref.4
	Mutagenesis	22	1	R → A: Lethal mitotic defects; when associated with A-7 and A-75. Lethal mitotic defects; when associated with A-7; A-11; A-12; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-11; A-12 and A-75. Ref.2 Ref.4
	Mutagenesis	54	1	K → A: Lethal mitotic defects; when associated with A-7; A-11; A-12; A-22; and A-56. Ref.4
	Mutagenesis	56	1	K → A: Lethal mitotic defects; when associated with A-7; A-11; A-12; A-22 and A-54. Ref.4
	Mutagenesis	75	1	R → A: Lethal mitotic defects; when associated with A-7 and A-22. Lethal mitotic defects; when associated with A-7; A-11; A-12 and A-22. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0DJH6 [UniParc]. Last modified May 16, 2012. Version 1. Checksum: B34E201BEBDE72F7	FASTA	76	8,743
	10 20 30 40 50 60 MRRTVPRGTL RKIIKKHKPH LRLAANTDLL VHLSFLLFLH RLAEEARTNA FENKSKIIKP 70 EHTIAAAKVI LKKSRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A comprehensive collection of chicken cDNAs." Boardman P.E., Sanz-Ezquerro J., Overton I.M., Burt D.W., Bosch E., Fong W.T., Tickle C., Brown W.R., Wilson S.A., Hubbard S.J. Curr. Biol. 12:1965-1969(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: White Leghorn Hisex.
[2]	"CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore." Hori T., Amano M., Suzuki A., Backer C.B., Welburn J.P., Dong Y., McEwen B.F., Shang W.-H., Suzuki E., Okawa K., Cheeseman I.M., Fukagawa T. Cell 135:1039-1052(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF ARG-7; ARG-11; LYS-12; ARG-22 AND ARG-75, SUBCELLULAR LOCATION.
[3]	"Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins." Suzuki A., Hori T., Nishino T., Usukura J., Miyagi A., Morikawa K., Fukagawa T. J. Cell Biol. 193:125-140(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CENPT.
[4]	"CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold." Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T. Cell 148:487-501(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-7; ARG-11; LYS-12; ARG-22; LYS-54 AND LYS-56.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BU413473 mRNA. No translation available.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3B0C	X-ray	2.20	W	1-76	[»]
3B0D	X-ray	2.20	C/W	1-76	[»]
3VH5	X-ray	2.40	W	1-76	[»]
3VH6	X-ray	3.35	W	1-76	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0DJH6. 3 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.20.10. 1 hit.

InterPro

IPR003958. CBFA_NFYB_domain.
IPR009072. Histone-fold.
[Graphical view]

Pfam

PF00808. CBFD_NFYB_HMF. 1 hit.
[Graphical view]

SUPFAM

SSF47113. Histone-fold. 1 hit.

ProtoNet

Search...

Entry information

Entry name

CENPW_CHICK

Accession

Primary (citable) accession number: P0DJH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2012
Last sequence update:	May 16, 2012
Last modified:	May 1, 2013
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program