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Protein

Centromere protein W

Gene

CENPW

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation (By similarity). The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres (By similarity). Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-GGA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein W
Short name:
CENP-W
Gene namesi
Name:CENPW
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7R → A: Lethal mitotic defects; when associated with A-22 and A-75. Lethal mitotic defects; when associated with A-11; A-12; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-11; A-12; A-22 and A-75. 2 Publications1
Mutagenesisi11R → A: Lethal mitotic defects; when associated with A-7; A-12; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-12; A-22 and A-75. 2 Publications1
Mutagenesisi12K → A: Lethal mitotic defects; when associated with A-7; A-11; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-11; A-22 and A-75. 2 Publications1
Mutagenesisi22R → A: Lethal mitotic defects; when associated with A-7 and A-75. Lethal mitotic defects; when associated with A-7; A-11; A-12; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-11; A-12 and A-75. 2 Publications1
Mutagenesisi54K → A: Lethal mitotic defects; when associated with A-7; A-11; A-12; A-22; and A-56. 1 Publication1
Mutagenesisi56K → A: Lethal mitotic defects; when associated with A-7; A-11; A-12; A-22 and A-54. 1 Publication1
Mutagenesisi75R → A: Lethal mitotic defects; when associated with A-7 and A-22. Lethal mitotic defects; when associated with A-7; A-11; A-12 and A-22. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004173841 – 76Centromere protein WAdd BLAST76

Expressioni

Gene expression databases

BgeeiENSGALG00000027454.
ExpressionAtlasiP0DJH6. baseline.

Interactioni

Subunit structurei

Heterodimer with CENPT; this dimer coassembles with CENPS-CENPX heterodimers at centromeres to form the tetrameric CENP-T-W-S-X complex, which is a subcomplex of the large constitutive centromere-associated network (CCAN, also known as the interphase centromere complex or ICEN) (PubMed:19070575, PubMed:21464230, PubMed:22304917). Interacts with NPM1 (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CENPTF1NPG54EBI-2132287,EBI-2132248

Protein-protein interaction databases

IntActiP0DJH6. 3 interactors.

Structurei

Secondary structure

176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 17Combined sources11
Helixi27 – 52Combined sources26
Beta strandi56 – 58Combined sources3
Helixi60 – 73Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B0CX-ray2.20W2-76[»]
3B0DX-ray2.20C/W2-76[»]
3VH5X-ray2.40W2-76[»]
3VH6X-ray3.35W2-76[»]
SMRiP0DJH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CENPW family.Curated

Phylogenomic databases

InParanoidiP0DJH6.
OrthoDBiEOG091G1B79.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiView protein in InterPro
IPR028847. CENP-W.
IPR009072. Histone-fold.
PfamiView protein in Pfam
PF15510. CENP-W. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

P0DJH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRTVPRGTL RKIIKKHKPH LRLAANTDLL VHLSFLLFLH RLAEEARTNA
60 70
FENKSKIIKP EHTIAAAKVI LKKSRG
Length:76
Mass (Da):8,743
Last modified:May 16, 2012 - v1
Checksum:iB34E201BEBDE72F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BU413473 mRNA. No translation available.
RefSeqiNP_001264662.1. NM_001277733.1.
UniGeneiGga.16138.

Genome annotation databases

EnsembliENSGALT00000045695; ENSGALP00000042097; ENSGALG00000027454.
GeneIDi421716.
KEGGigga:421716.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BU413473 mRNA. No translation available.
RefSeqiNP_001264662.1. NM_001277733.1.
UniGeneiGga.16138.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B0CX-ray2.20W2-76[»]
3B0DX-ray2.20C/W2-76[»]
3VH5X-ray2.40W2-76[»]
3VH6X-ray3.35W2-76[»]
SMRiP0DJH6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0DJH6. 3 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000045695; ENSGALP00000042097; ENSGALG00000027454.
GeneIDi421716.
KEGGigga:421716.

Organism-specific databases

CTDi387103.

Phylogenomic databases

InParanoidiP0DJH6.
OrthoDBiEOG091G1B79.

Enzyme and pathway databases

ReactomeiR-GGA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.

Miscellaneous databases

PROiP0DJH6.

Gene expression databases

BgeeiENSGALG00000027454.
ExpressionAtlasiP0DJH6. baseline.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiView protein in InterPro
IPR028847. CENP-W.
IPR009072. Histone-fold.
PfamiView protein in Pfam
PF15510. CENP-W. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCENPW_CHICK
AccessioniPrimary (citable) accession number: P0DJH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: May 16, 2012
Last modified: March 15, 2017
This is version 35 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Association with CENPA-containing complexes may be indirect and due to the proximity of centromeric nucleosomes containing histone H3 with those containing CENPA.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.