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Protein

Centromere protein W

Gene

CENPW

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation (By similarity). The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres (By similarity). Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.By similarity2 Publications

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. kinetochore assembly Source: Ensembl
  3. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_339698. Deposition of new CENPA-containing nucleosomes at the centromere.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein W
Short name:
CENP-W
Gene namesi
Name:CENPW
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Unplaced

Subcellular locationi

Nucleus. Chromosomecentromerekinetochore
Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore.

GO - Cellular componenti

  1. condensed chromosome kinetochore Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi7 – 71R → A: Lethal mitotic defects; when associated with A-22 and A-75. Lethal mitotic defects; when associated with A-11; A-12; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-11; A-12; A-22 and A-75. 2 Publications
Mutagenesisi11 – 111R → A: Lethal mitotic defects; when associated with A-7; A-12; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-12; A-22 and A-75. 2 Publications
Mutagenesisi12 – 121K → A: Lethal mitotic defects; when associated with A-7; A-11; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-11; A-22 and A-75. 2 Publications
Mutagenesisi22 – 221R → A: Lethal mitotic defects; when associated with A-7 and A-75. Lethal mitotic defects; when associated with A-7; A-11; A-12; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-11; A-12 and A-75. 2 Publications
Mutagenesisi54 – 541K → A: Lethal mitotic defects; when associated with A-7; A-11; A-12; A-22; and A-56. 1 Publication
Mutagenesisi56 – 561K → A: Lethal mitotic defects; when associated with A-7; A-11; A-12; A-22 and A-54. 1 Publication
Mutagenesisi75 – 751R → A: Lethal mitotic defects; when associated with A-7 and A-22. Lethal mitotic defects; when associated with A-7; A-11; A-12 and A-22. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7676Centromere protein WPRO_0000417384Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiP0DJH6. baseline.

Interactioni

Subunit structurei

Part of a centromere complex consisting of CENPA, CENPT and CENPW (By similarity). Part of a centromere complex consisting of histone H3, CENPT and CENPW. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW. Interacts directly with CENPT. Binds DNA.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CENPTF1NPG54EBI-2132287,EBI-2132248

Protein-protein interaction databases

IntActiP0DJH6. 3 interactions.

Structurei

Secondary structure

1
76
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1711Combined sources
Helixi27 – 5226Combined sources
Beta strandi56 – 583Combined sources
Helixi60 – 7314Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B0CX-ray2.20W2-76[»]
3B0DX-ray2.20C/W2-76[»]
3VH5X-ray2.40W2-76[»]
3VH6X-ray3.35W2-76[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CENPW family.Curated

Phylogenomic databases

InParanoidiP0DJH6.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR028847. CENP-W.
IPR009072. Histone-fold.
[Graphical view]
PfamiPF15510. CENP-W. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

P0DJH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRTVPRGTL RKIIKKHKPH LRLAANTDLL VHLSFLLFLH RLAEEARTNA
60 70
FENKSKIIKP EHTIAAAKVI LKKSRG
Length:76
Mass (Da):8,743
Last modified:May 15, 2012 - v1
Checksum:iB34E201BEBDE72F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BU413473 mRNA. No translation available.
RefSeqiNP_001264662.1. NM_001277733.1.
UniGeneiGga.16138.

Genome annotation databases

GeneIDi421716.
KEGGigga:421716.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BU413473 mRNA. No translation available.
RefSeqiNP_001264662.1. NM_001277733.1.
UniGeneiGga.16138.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B0CX-ray2.20W2-76[»]
3B0DX-ray2.20C/W2-76[»]
3VH5X-ray2.40W2-76[»]
3VH6X-ray3.35W2-76[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0DJH6. 3 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi421716.
KEGGigga:421716.

Organism-specific databases

CTDi387103.

Phylogenomic databases

InParanoidiP0DJH6.

Enzyme and pathway databases

ReactomeiREACT_339698. Deposition of new CENPA-containing nucleosomes at the centromere.

Gene expression databases

ExpressionAtlasiP0DJH6. baseline.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR028847. CENP-W.
IPR009072. Histone-fold.
[Graphical view]
PfamiPF15510. CENP-W. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: White Leghorn Hisex.
  2. "CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore."
    Hori T., Amano M., Suzuki A., Backer C.B., Welburn J.P., Dong Y., McEwen B.F., Shang W.-H., Suzuki E., Okawa K., Cheeseman I.M., Fukagawa T.
    Cell 135:1039-1052(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF ARG-7; ARG-11; LYS-12; ARG-22 AND ARG-75, SUBCELLULAR LOCATION.
  3. "Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins."
    Suzuki A., Hori T., Nishino T., Usukura J., Miyagi A., Morikawa K., Fukagawa T.
    J. Cell Biol. 193:125-140(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CENPT.
  4. "CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold."
    Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T.
    Cell 148:487-501(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-7; ARG-11; LYS-12; ARG-22; LYS-54 AND LYS-56.

Entry informationi

Entry nameiCENPW_CHICK
AccessioniPrimary (citable) accession number: P0DJH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2012
Last sequence update: May 15, 2012
Last modified: March 31, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Association with CENPA-containing complexes may be indirect and due to the proximity of centromeric nucleosomes containing histone H3 with those containing CENPA.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.