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P0DJH6

- CENPW_CHICK

UniProt

P0DJH6 - CENPW_CHICK

Protein

Centromere protein W

Gene

CENPW

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation By similarity. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres By similarity. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.By similarity2 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. kinetochore assembly Source: Ensembl
    2. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Centromere protein W
    Short name:
    CENP-W
    Gene namesi
    Name:CENPW
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Nucleus. Chromosomecentromerekinetochore
    Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore.

    GO - Cellular componenti

    1. condensed chromosome kinetochore Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi7 – 71R → A: Lethal mitotic defects; when associated with A-22 and A-75. Lethal mitotic defects; when associated with A-11; A-12; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-11; A-12; A-22 and A-75. 2 Publications
    Mutagenesisi11 – 111R → A: Lethal mitotic defects; when associated with A-7; A-12; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-12; A-22 and A-75. 2 Publications
    Mutagenesisi12 – 121K → A: Lethal mitotic defects; when associated with A-7; A-11; A-22; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-11; A-22 and A-75. 2 Publications
    Mutagenesisi22 – 221R → A: Lethal mitotic defects; when associated with A-7 and A-75. Lethal mitotic defects; when associated with A-7; A-11; A-12; A-54 and A-56. Lethal mitotic defects; when associated with A-7; A-11; A-12 and A-75. 2 Publications
    Mutagenesisi54 – 541K → A: Lethal mitotic defects; when associated with A-7; A-11; A-12; A-22; and A-56. 1 Publication
    Mutagenesisi56 – 561K → A: Lethal mitotic defects; when associated with A-7; A-11; A-12; A-22 and A-54. 1 Publication
    Mutagenesisi75 – 751R → A: Lethal mitotic defects; when associated with A-7 and A-22. Lethal mitotic defects; when associated with A-7; A-11; A-12 and A-22. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7676Centromere protein WPRO_0000417384Add
    BLAST

    Interactioni

    Subunit structurei

    Part of a centromere complex consisting of CENPA, CENPT and CENPW By similarity. Part of a centromere complex consisting of histone H3, CENPT and CENPW. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW. Interacts directly with CENPT. Binds DNA.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CENPTF1NPG54EBI-2132287,EBI-2132248

    Protein-protein interaction databases

    IntActiP0DJH6. 3 interactions.

    Structurei

    Secondary structure

    1
    76
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 1711
    Helixi27 – 5226
    Beta strandi56 – 583
    Helixi60 – 7314

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3B0CX-ray2.20W2-76[»]
    3B0DX-ray2.20C/W2-76[»]
    3VH5X-ray2.40W2-76[»]
    3VH6X-ray3.35W2-76[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CENPW family.Curated

    Family and domain databases

    Gene3Di1.10.20.10. 1 hit.
    InterProiIPR028847. CENP-W.
    IPR009072. Histone-fold.
    [Graphical view]
    PfamiPF15510. CENP-W. 1 hit.
    [Graphical view]
    SUPFAMiSSF47113. SSF47113. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0DJH6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRTVPRGTL RKIIKKHKPH LRLAANTDLL VHLSFLLFLH RLAEEARTNA   50
    FENKSKIIKP EHTIAAAKVI LKKSRG 76
    Length:76
    Mass (Da):8,743
    Last modified:May 16, 2012 - v1
    Checksum:iB34E201BEBDE72F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BU413473 mRNA. No translation available.
    RefSeqiNP_001264662.1. NM_001277733.1.
    UniGeneiGga.16138.

    Genome annotation databases

    GeneIDi421716.
    KEGGigga:421716.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BU413473 mRNA. No translation available.
    RefSeqi NP_001264662.1. NM_001277733.1.
    UniGenei Gga.16138.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3B0C X-ray 2.20 W 2-76 [» ]
    3B0D X-ray 2.20 C/W 2-76 [» ]
    3VH5 X-ray 2.40 W 2-76 [» ]
    3VH6 X-ray 3.35 W 2-76 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0DJH6. 3 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 421716.
    KEGGi gga:421716.

    Organism-specific databases

    CTDi 387103.

    Family and domain databases

    Gene3Di 1.10.20.10. 1 hit.
    InterProi IPR028847. CENP-W.
    IPR009072. Histone-fold.
    [Graphical view ]
    Pfami PF15510. CENP-W. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47113. SSF47113. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: White Leghorn Hisex.
    2. "CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore."
      Hori T., Amano M., Suzuki A., Backer C.B., Welburn J.P., Dong Y., McEwen B.F., Shang W.-H., Suzuki E., Okawa K., Cheeseman I.M., Fukagawa T.
      Cell 135:1039-1052(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF ARG-7; ARG-11; LYS-12; ARG-22 AND ARG-75, SUBCELLULAR LOCATION.
    3. "Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins."
      Suzuki A., Hori T., Nishino T., Usukura J., Miyagi A., Morikawa K., Fukagawa T.
      J. Cell Biol. 193:125-140(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CENPT.
    4. "CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold."
      Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T.
      Cell 148:487-501(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-7; ARG-11; LYS-12; ARG-22; LYS-54 AND LYS-56.

    Entry informationi

    Entry nameiCENPW_CHICK
    AccessioniPrimary (citable) accession number: P0DJH6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2012
    Last sequence update: May 16, 2012
    Last modified: October 1, 2014
    This is version 18 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Association with CENPA-containing complexes may be indirect and due to the proximity of centromeric nucleosomes containing histone H3 with those containing CENPA.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3