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Protein

Pepsin A-5

Gene

PGA5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Catalytic activityi

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei94PROSITE-ProRule annotation1 Publication1
Active sitei277PROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Digestion

Enzyme and pathway databases

BioCyciZFISH:HS11540-MONOMER.
ReactomeiR-HSA-5683826. Surfactant metabolism.

Protein family/group databases

MEROPSiA01.071.

Names & Taxonomyi

Protein namesi
Recommended name:
Pepsin A-5 (EC:3.4.23.1)
Alternative name(s):
Pepsinogen-5
Gene namesi
Name:PGA5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:8887. PGA5.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • multivesicular body lumen Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi5222.
OpenTargetsiENSG00000256713.
PharmGKBiPA33224.

Chemistry databases

ChEMBLiCHEMBL3295.
GuidetoPHARMACOLOGYi2390.

Polymorphism and mutation databases

BioMutaiPGA5.
DMDMi378522017.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 152 PublicationsAdd BLAST15
PropeptideiPRO_000041575916 – 62Activation peptideAdd BLAST47
ChainiPRO_000041576063 – 388Pepsin A-5Add BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi107 ↔ 1121 Publication
Modified residuei130PhosphoserineBy similarity1
Disulfide bondi268 ↔ 2721 Publication
Disulfide bondi311 ↔ 3441 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiP0DJD9.
PeptideAtlasiP0DJD9.
PRIDEiP0DJD9.

PTM databases

iPTMnetiP0DJD9.
PhosphoSitePlusiP0DJD9.

Expressioni

Gene expression databases

CleanExiHS_PGA5.
ExpressionAtlasiP0DJD9. baseline and differential.
GenevisibleiP0DJD9. HS.

Organism-specific databases

HPAiHPA046875.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000309542.

Chemistry databases

BindingDBiP0DJD9.

Structurei

3D structure databases

ProteinModelPortaliP0DJD9.
SMRiP0DJD9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini76 – 385Peptidase A1PROSITE-ProRule annotationAdd BLAST310

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
InParanoidiP0DJD9.
KOiK06002.
OMAiFQGMNIP.
OrthoDBiEOG091G0JP7.
PhylomeDBiP0DJD9.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0DJD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWLLLLGLV ALSECIMYKV PLIRKKSLRR TLSERGLLKD FLKKHNLNPA
60 70 80 90 100
RKYFPQWEAP TLVDEQPLEN YLDMEYFGTI GIGTPAQDFT VVFDTGSSNL
110 120 130 140 150
WVPSVYCSSL ACTNHNRFNP EDSSTYQSTS ETVSITYGTG SMTGILGYDT
160 170 180 190 200
VQVGGISDTN QIFGLSETEP GSFLYYAPFD GILGLAYPSI SSSGATPVFD
210 220 230 240 250
NIWNQGLVSQ DLFSVYLSAD DKSGSVVIFG GIDSSYYTGS LNWVPVTVEG
260 270 280 290 300
YWQITVDSIT MNGETIACAE GCQAIVDTGT SLLTGPTSPI ANIQSDIGAS
310 320 330 340 350
ENSDGDMVVS CSAISSLPDI VFTINGVQYP VPPSAYILQS EGSCISGFQG
360 370 380
MNVPTESGEL WILGDVFIRQ YFTVFDRANN QVGLAPVA
Length:388
Mass (Da):41,993
Last modified:February 22, 2012 - v1
Checksum:i2C456A75DA96CDDA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28L → F in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti58E → K in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti92V → L in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti222K → Q in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti265T → A in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti353V → L in AAA60061 (PubMed:2714789).Curated1
Sequence conflicti371 – 372YF → FY AA sequence (PubMed:4909888).Curated2
Sequence conflicti376D → E in AAA60061 (PubMed:2714789).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26032
, M26025, M26026, M26027, M26028, M26029, M26030, M26031 Genomic DNA. Translation: AAA60061.1.
AP003037 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73928.1.
BC029055 mRNA. Translation: AAH29055.1.
BC146999 mRNA. Translation: AAI47000.1.
BC171889 mRNA. Translation: AAI71889.1.
CCDSiCCDS8001.1.
PIRiA00980. PEHU.
A30142.
A92058.
B30142.
RefSeqiNP_055039.1. NM_014224.4.
UniGeneiHs.432854.

Genome annotation databases

EnsembliENST00000312403; ENSP00000309542; ENSG00000256713.
GeneIDi5222.
KEGGihsa:5222.
UCSCiuc001nqz.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26032
, M26025, M26026, M26027, M26028, M26029, M26030, M26031 Genomic DNA. Translation: AAA60061.1.
AP003037 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73928.1.
BC029055 mRNA. Translation: AAH29055.1.
BC146999 mRNA. Translation: AAI47000.1.
BC171889 mRNA. Translation: AAI71889.1.
CCDSiCCDS8001.1.
PIRiA00980. PEHU.
A30142.
A92058.
B30142.
RefSeqiNP_055039.1. NM_014224.4.
UniGeneiHs.432854.

3D structure databases

ProteinModelPortaliP0DJD9.
SMRiP0DJD9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000309542.

Chemistry databases

BindingDBiP0DJD9.
ChEMBLiCHEMBL3295.
GuidetoPHARMACOLOGYi2390.

Protein family/group databases

MEROPSiA01.071.

PTM databases

iPTMnetiP0DJD9.
PhosphoSitePlusiP0DJD9.

Polymorphism and mutation databases

BioMutaiPGA5.
DMDMi378522017.

Proteomic databases

PaxDbiP0DJD9.
PeptideAtlasiP0DJD9.
PRIDEiP0DJD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312403; ENSP00000309542; ENSG00000256713.
GeneIDi5222.
KEGGihsa:5222.
UCSCiuc001nqz.4. human.

Organism-specific databases

CTDi5222.
DisGeNETi5222.
GeneCardsiPGA5.
HGNCiHGNC:8887. PGA5.
HPAiHPA046875.
MIMi169700. gene.
169730. gene.
neXtProtiNX_P0DJD9.
OpenTargetsiENSG00000256713.
PharmGKBiPA33224.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
InParanoidiP0DJD9.
KOiK06002.
OMAiFQGMNIP.
OrthoDBiEOG091G0JP7.
PhylomeDBiP0DJD9.
TreeFamiTF314990.

Enzyme and pathway databases

BioCyciZFISH:HS11540-MONOMER.
ReactomeiR-HSA-5683826. Surfactant metabolism.

Miscellaneous databases

GeneWikiiPGA5.
GenomeRNAii5222.
PROiP0DJD9.
SOURCEiSearch...

Gene expression databases

CleanExiHS_PGA5.
ExpressionAtlasiP0DJD9. baseline and differential.
GenevisibleiP0DJD9. HS.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEPA5_HUMAN
AccessioniPrimary (citable) accession number: P0DJD9
Secondary accession number(s): A8K749
, B7ZW62, B7ZW75, P00790, Q7M4R0, Q8N1E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: February 22, 2012
Last modified: November 2, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.