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P0DJD8

- PEPA3_HUMAN

UniProt

P0DJD8 - PEPA3_HUMAN

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Protein
Pepsin A-3
Gene
PGA3
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Catalytic activityi

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei94 – 9411 Publication
Active sitei277 – 27711 Publication

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Digestion

Names & Taxonomyi

Protein namesi
Recommended name:
Pepsin A-3 (EC:3.4.23.1)
Alternative name(s):
Pepsinogen-3
Gene namesi
Name:PGA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:8885. PGA3.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 15152 Publications
Add
BLAST
Propeptidei16 – 6247Activation peptide
PRO_0000026013Add
BLAST
Chaini63 – 388326Pepsin A-3
PRO_0000026014Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi107 ↔ 1121 Publication
Disulfide bondi268 ↔ 2721 Publication
Disulfide bondi311 ↔ 3441 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiP0DJD8.

PTM databases

PhosphoSiteiP0DJD8.

Expressioni

Gene expression databases

BgeeiP0DJD8.
CleanExiHS_PGA3.

Organism-specific databases

HPAiCAB026358.
HPA046875.

Structurei

3D structure databases

ProteinModelPortaliP0DJD8.
SMRiP0DJD8. Positions 17-388.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A1 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG248684.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
KOiK06002.
OMAiXNDESGS.
OrthoDBiEOG7HQN88.
PhylomeDBiP0DJD8.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0DJD8-1 [UniParc]FASTAAdd to Basket

« Hide

MKWLLLLGLV ALSECIMYKV PLIRKKSLRR TLSERGLLKD FLKKHNLNPA    50
RKYFPQWKAP TLVDEQPLEN YLDMEYFGTI GIGTPAQDFT VVFDTGSSNL 100
WVPSVYCSSL ACTNHNRFNP EDSSTYQSTS ETVSITYGTG SMTGILGYDT 150
VQVGGISDTN QIFGLSETEP GSFLYYAPFD GILGLAYPSI SSSGATPVFD 200
NIWNQGLVSQ DLFSVYLSAD DQSGSVVIFG GIDSSYYTGS LNWVPVTVEG 250
YWQITVDSIT MNGEAIACAE GCQAIVDTGT SLLTGPTSPI ANIQSDIGAS 300
ENSDGDMVVS CSAISSLPDI VFTINGVQYP VPPSAYILQS EGSCISGFQG 350
MNLPTESGEL WILGDVFIRQ YFTVFDRANN QVGLAPVA 388
Length:388
Mass (Da):41,976
Last modified:February 22, 2012 - v1
Checksum:i5DA16E41FE198582
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921V → L in BAG35783. 1 Publication
Sequence conflicti92 – 921V → L in BAG54225. 1 Publication
Sequence conflicti92 – 921V → L in AAI71815. 1 Publication
Sequence conflicti371 – 3722YF → FY AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK312941 mRNA. Translation: BAG35783.1.
AK125628 mRNA. Translation: BAG54225.1.
AP000437 Genomic DNA. No translation available.
BC171815 mRNA. Translation: AAI71815.1.
CCDSiCCDS31574.1.
PIRiA00980. PEHU.
A30142.
A92058.
B30142.
RefSeqiNP_001073275.1. NM_001079807.2.
XP_005276461.1. XM_005276404.1.
UniGeneiHs.601055.
Hs.661883.

Genome annotation databases

EnsembliENST00000325558; ENSP00000322192; ENSG00000229859.
GeneIDi101929842.
643834.
KEGGihsa:101929842.
hsa:643834.
UCSCiuc001nqx.3. human.

Polymorphism databases

DMDMi378521956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK312941 mRNA. Translation: BAG35783.1 .
AK125628 mRNA. Translation: BAG54225.1 .
AP000437 Genomic DNA. No translation available.
BC171815 mRNA. Translation: AAI71815.1 .
CCDSi CCDS31574.1.
PIRi A00980. PEHU.
A30142.
A92058.
B30142.
RefSeqi NP_001073275.1. NM_001079807.2.
XP_005276461.1. XM_005276404.1.
UniGenei Hs.601055.
Hs.661883.

3D structure databases

ProteinModelPortali P0DJD8.
SMRi P0DJD8. Positions 17-388.
ModBasei Search...

PTM databases

PhosphoSitei P0DJD8.

Polymorphism databases

DMDMi 378521956.

Proteomic databases

PRIDEi P0DJD8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000325558 ; ENSP00000322192 ; ENSG00000229859 .
GeneIDi 101929842.
643834.
KEGGi hsa:101929842.
hsa:643834.
UCSCi uc001nqx.3. human.

Organism-specific databases

CTDi 643834.
GeneCardsi GC11P060970.
HGNCi HGNC:8885. PGA3.
HPAi CAB026358.
HPA046875.
MIMi 169700. gene.
169710. gene.
neXtProti NX_P0DJD8.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG248684.
HOGENOMi HOG000197681.
HOVERGENi HBG000482.
KOi K06002.
OMAi XNDESGS.
OrthoDBi EOG7HQN88.
PhylomeDBi P0DJD8.
TreeFami TF314990.

Miscellaneous databases

ChiTaRSi PGA3. human.
NextBioi 115445.
PROi P0DJD8.
SOURCEi Search...

Gene expression databases

Bgeei P0DJD8.
CleanExi HS_PGA3.

Family and domain databases

Gene3Di 2.40.70.10. 2 hits.
InterProi IPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view ]
PANTHERi PTHR13683. PTHR13683. 1 hit.
Pfami PF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view ]
PRINTSi PR00792. PEPSIN.
SUPFAMi SSF50630. SSF50630. 1 hit.
PROSITEi PS00141. ASP_PROTEASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Stomach.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Isolation of human, swine, and rat prepepsinogens and calf preprochymosin, and determination of the primary structures of their NH2-terminal signal sequences."
    Ichihara Y., Sogawa K., Takahashi K.
    J. Biochem. 98:483-492(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE OF 1-28.
  5. "A comparative study on the NH2-terminal amino acid sequences and some other properties of six isozymic forms of human pepsinogens and pepsins."
    Athauda S.B.P., Tanji M., Kageyama T., Takahashi K.
    J. Biochem. 106:920-927(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-100.
  6. "Activation of human pepsinogens."
    Foltmann B.
    FEBS Lett. 241:69-72(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-68.
  7. "Carboxyl-terminal sequence of human gastricsin and pepsin."
    Huang W.-Y., Tang J.
    J. Biol. Chem. 245:2189-2193(1970) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 362-388.
  8. "Primary structure of human pepsinogen gene."
    Sogawa K., Fujii-Kuriyama Y., Mizukami Y., Ichihara Y., Takahashi K.
    J. Biol. Chem. 258:5306-5311(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  9. "Nucleotide sequence comparison of five human pepsinogen A (PGA) genes: evolution of the PGA multigene family."
    Evers M.P.J., Zelle B., Bebelman J.-P., van Beusechem V., Kraakman L., Hoffer M.J.V., Pronk J.C., Mager W.H., Planta R.J., Eriksson A.W., Frants R.R.
    Genomics 4:232-239(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
    Tissue: Placenta.
  10. "Crystal structure of human pepsin and its complex with pepstatin."
    Fujinaga M., Chernaia M.M., Tarasova N.I., Mosimann S.C., James M.N.G.
    Protein Sci. 4:960-972(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 63-388, ACTIVE SITES, DISULFIDE BONDS.

Entry informationi

Entry nameiPEPA3_HUMAN
AccessioniPrimary (citable) accession number: P0DJD8
Secondary accession number(s): A8K749
, B2R7D6, B7ZW75, P00790, Q7M4R0, Q8N1E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: February 22, 2012
Last modified: September 3, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi