P0DJD7A8K749B7ZW75P00790Q7M4R0Q8N1E3PEPA4_HUMANPepsin A-43.4.23.1Pepsinogen-4PGA4Homo sapiensHumanEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomoPrimary structure of human pepsinogen gene.NUCLEOTIDE SEQUENCE [GENOMIC DNA]Complete sequencing and characterization of 21,243 full-length human cDNAs.NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]Human chromosome 11 DNA sequence and analysis including novel gene identification.NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]Isolation of human, swine, and rat prepepsinogens and calf preprochymosin, and determination of the primary structures of their NH2-terminal signal sequences.PARTIAL PROTEIN SEQUENCE OF 1-28A comparative study on the NH2-terminal amino acid sequences and some other properties of six isozymic forms of human pepsinogens and pepsins.PROTEIN SEQUENCE OF 16-100Activation of human pepsinogens.PROTEIN SEQUENCE OF 16-68Carboxyl-terminal sequence of human gastricsin and pepsin.PROTEIN SEQUENCE OF 362-388Nucleotide sequence comparison of five human pepsinogen A (PGA) genes: evolution of the PGA multigene family.IDENTIFICATIONCrystal structure of human pepsin and its complex with pepstatin.X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 63-388ACTIVE SITESDISULFIDE BONDSShows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.P0DJD7Q86WK6false3P0DJD7P04233-2false3P0DJD7Q96LL3false3P0DJD7Q8TBE3false3P0DJD7Q9UM44false3P0DJD7P15151false3P0DJD7Q9NPE6false3P0DJD7P27105false3P0DJD7Q9BVX2false3SecretedBelongs to the peptidase A1 family.3D-structureAspartyl proteaseDigestionDirect protein sequencingDisulfide bondHydrolasePhosphoproteinProteaseReference proteomeSecretedSignalZymogenATYFFYMKWLLLLGLVALSECIMYKVPLIRKKSLRRTLSERGLLKDFLKKHNLNPARKYFPQWEAPTLVDEQPLENYLDMEYFGTIGIGTPAQDFTVVFDTGSSNLWVPSVYCSSLACTNHNRFNPEDSSTYQSTSETVSITYGTGSMTGILGYDTVQVGGISDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISSSGATPVFDNIWNQGLVSQDLFSVYLSADDQSGSVVIFGGIDSSYYTGSLNWVPVTVEGYWQITVDSITMNGEAIACAEGCQAIVDTGTSLLTGPTSPIANIQSDIGASENSDGDMVVSCSAISSLPDIVFTINGVQYPVPPSAYILQSEGSCISGFQGMNLPTESGELWILGDVFIRQYFTVFDRANNQVGLAPVA
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