UniProtKB - P0DJ47 (VKT12_MESMA)
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Protein
Kunitz-type serine protease inhibitor BmKTT-3
Gene
N/A
Organism
Mesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Status
Functioni
Dual-function toxin that inhibits 85% of the activity of trypsin at a molar ratio of 4:1 with a dissociation constant of 760 nM, and that inhibits mKv1.3/KCNA3 potassium channel currents, but very weakly.1 Publication
Miscellaneous
Has no effect on chymotrypsin and elastase.1 Publication
Sites
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Sitei | 17 – 18 | Reactive bond for trypsinBy similarity | 2 |
GO - Molecular functioni
- serine-type endopeptidase inhibitor activity Source: UniProtKB
GO - Biological processi
- envenomation resulting in negative regulation of voltage-gated potassium channel activity in other organism Source: UniProtKB
Keywordsi
| Molecular function | Ion channel impairing toxin, Potassium channel impairing toxin, Protease inhibitor, Serine protease inhibitor, Toxin, Voltage-gated potassium channel impairing toxin |
Names & Taxonomyi
| Protein namesi | Recommended name: Kunitz-type serine protease inhibitor BmKTT-3Alternative name(s): Delta-KTx 1.2 |
| Organismi | Mesobuthus martensii (Manchurian scorpion) (Buthus martensii) |
| Taxonomic identifieri | 34649 [NCBI] |
| Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus |
Subcellular locationi
- Secreted By similarity
GO - Cellular componenti
- extracellular region Source: UniProtKB
- host cell membrane Source: UniProtKB
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| ChainiPRO_0000418101 | 1 – 70 | Kunitz-type serine protease inhibitor BmKTT-3Add BLAST | 70 |
Amino acid modifications
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Disulfide bondi | 7 ↔ 57 | PROSITE-ProRule annotation | ||
| Disulfide bondi | 16 ↔ 40 | PROSITE-ProRule annotation | ||
| Disulfide bondi | 32 ↔ 53 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.
Family & Domainsi
Domains and Repeats
| Feature key | Position(s) | DescriptionActions | Graphical view | Length |
|---|---|---|---|---|
| Domaini | 7 – 57 | BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd BLAST | 51 |
Sequence similaritiesi
Belongs to the venom Kunitz-type family. Scorpion delta-Ktx subfamily. Delta-Ktx 1 sub-subfamily.Curated
Family and domain databases
| Gene3Di | 4.10.410.10. 1 hit. |
| InterProi | View protein in InterPro IPR002223. Kunitz_BPTI. IPR020901. Prtase_inh_Kunz-CS. |
| Pfami | View protein in Pfam PF00014. Kunitz_BPTI. 1 hit. |
| PRINTSi | PR00759. BASICPTASE. |
| SMARTi | View protein in SMART SM00131. KU. 1 hit. |
| SUPFAMi | SSF57362. SSF57362. 1 hit. |
| PROSITEi | View protein in PROSITE PS00280. BPTI_KUNITZ_1. 1 hit. PS50279. BPTI_KUNITZ_2. 1 hit. |
Sequencei
Sequence statusi: Complete.
P0DJ47-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
KHGSINCRLP PERGPCRGNI TKYYYHNESR TCRTFSYGGC EGNSNNFRNR
60 70
HYCMKYCARK RHGWLGTGWI
Similar proteinsi
Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:| 100% | UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry. |
| 90% | UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence). |
| 50% | UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster. |
Entry informationi
| Entry namei | VKT12_MESMA | |
| Accessioni | P0DJ47Primary (citable) accession number: P0DJ47 | |
| Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2012 |
| Last sequence update: | July 11, 2012 | |
| Last modified: | May 10, 2017 | |
| This is version 17 of the entry and version 1 of the sequence. See complete history. | ||
| Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
| Annotation program | Animal Toxin Annotation Program | |
Miscellaneousi
Documents
- Scorpion potassium channel toxins
Nomenclature of scorpion potassium channel toxins and list of entries - SIMILARITY comments
Index of protein domains and families
