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P0DI97 (NRX1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neurexin-1-beta
Alternative name(s):
Neurexin I-beta
Gene names
Name:Nrxn1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Neuronal cell surface protein that may be involved in cell recognition and cell adhesion by forming intracellular junctions through binding to neuroligins. May play a role in formation or maintenance of synaptic junctions. May mediate intracellular signaling. May play a role in angiogenesis By similarity.

Subunit structure

The cytoplasmic C-terminal region binds to CASK. Isoforms 3b and 4b bind neuroligins NLGN1, NLGN2 and NLGN3, alpha-dystroglycan and alpha-latrotoxin. Binding to neuroligins is calcium-dependent, and the binding preference ranks as follow: NLGN1 > NLGN4 >> NLGN3 > NLGN2 By similarity. Interacts with CBLN1, CBLN2 and more weakly with CBLN4. CBLN1-binding is calcium-independent. Ref.2 Ref.3

Subcellular location

Cell membrane; Single-pass type I membrane protein. Cell junctionsynapse. Note: Localized on the pre-synaptic membrane By similarity.

Post-translational modification

Highly O-glycosylated and minor N-glycosylated By similarity.

Sequence similarities

Belongs to the neurexin family.

Contains 1 laminin G-like domain.

Ontologies

Keywords
   Biological processAngiogenesis
Cell adhesion
   Cellular componentCell junction
Cell membrane
Membrane
Synapse
   Coding sequence diversityAlternative promoter usage
Alternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   LigandCalcium
Metal-binding
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processN-methyl-D-aspartate receptor clustering

Inferred from direct assay PubMed 15620359. Source: BHF-UCL

angiogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

calcium-dependent cell-cell adhesion

Inferred from direct assay PubMed 12796785. Source: BHF-UCL

cerebellar granule cell differentiation

Inferred from genetic interaction PubMed 20537373. Source: BHF-UCL

establishment of protein localization

Inferred from direct assay PubMed 12796785. Source: BHF-UCL

gamma-aminobutyric acid receptor clustering

Inferred from direct assay PubMed 15620359. Source: BHF-UCL

gephyrin clustering

Inferred from direct assay PubMed 15620359. Source: BHF-UCL

guanylate kinase-associated protein clustering

Inferred from direct assay PubMed 15620359. Source: BHF-UCL

heterophilic cell-cell adhesion

Inferred from direct assay PubMed 12796785. Source: BHF-UCL

negative regulation of filopodium assembly

Inferred from direct assay PubMed 20869594. Source: BHF-UCL

neuroligin clustering

Inferred from direct assay PubMed 15620359. Source: BHF-UCL

neuron cell-cell adhesion

Inferred from direct assay PubMed 12796785. Source: BHF-UCL

neuron maturation

Inferred from direct assay PubMed 15620359. Source: BHF-UCL

neurotransmitter secretion

Inferred from genetic interaction PubMed 12827191. Source: MGI

positive regulation of establishment of protein localization to plasma membrane

Inferred from direct assay PubMed 19098102. Source: BHF-UCL

positive regulation of excitatory postsynaptic membrane potential

Inferred from direct assay PubMed 19098102. Source: BHF-UCL

positive regulation of synapse assembly

Inferred from genetic interaction PubMed 21356198. Source: MGI

positive regulation of synapse maturation

Inferred from direct assay PubMed 19567877. Source: MGI

positive regulation of synaptic transmission, glutamatergic

Inferred from direct assay PubMed 19098102. Source: BHF-UCL

postsynaptic density protein 95 clustering

Inferred from direct assay PubMed 15620359. Source: BHF-UCL

postsynaptic membrane assembly

Inferred from direct assay PubMed 15620359. Source: BHF-UCL

presynaptic membrane assembly

Inferred from direct assay PubMed 12796785. Source: BHF-UCL

protein localization to synapse

Inferred from direct assay PubMed 12796785. Source: BHF-UCL

receptor localization to synapse

Inferred from direct assay PubMed 15620359. Source: BHF-UCL

regulation of N-methyl-D-aspartate selective glutamate receptor activity

Inferred from direct assay PubMed 19098102. Source: BHF-UCL

regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity

Inferred from direct assay PubMed 19098102PubMed 20869594. Source: BHF-UCL

regulation of insulin secretion involved in cellular response to glucose stimulus

Inferred from mutant phenotype PubMed 22235116. Source: MGI

synapse assembly

Inferred from genetic interaction PubMed 12827191. Source: MGI

synaptic transmission

Inferred from genetic interaction PubMed 12827191. Source: MGI

synaptic vesicle clustering

Inferred from direct assay PubMed 12796785. Source: BHF-UCL

   Cellular_componentaxonal growth cone

Inferred from direct assay PubMed 12796785. Source: BHF-UCL

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cell surface

Inferred from direct assay PubMed 19098102. Source: BHF-UCL

endocytic vesicle

Inferred from sequence or structural similarity. Source: BHF-UCL

excitatory synapse

Inferred by curator PubMed 15620359. Source: BHF-UCL

inhibitory synapse

Inferred by curator PubMed 15620359. Source: BHF-UCL

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

presynaptic membrane

Inferred from direct assay PubMed 12827191. Source: MGI

   Molecular_functionacetylcholine receptor binding

Inferred from direct assay PubMed 19567877. Source: MGI

calcium channel regulator activity

Inferred from genetic interaction PubMed 12827191. Source: MGI

calcium-dependent protein binding

Inferred from direct assay PubMed 19098102. Source: BHF-UCL

cell adhesion molecule binding

Inferred from physical interaction PubMed 12796785PubMed 19098102. Source: BHF-UCL

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

neuroligin family protein binding

Inferred from physical interaction PubMed 12796785PubMed 19098102. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Cbln1Q9R1714EBI-2794440,EBI-2794140

Alternative products

This entry describes 5 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]

Note: A number of isoforms, alpha-type and beta-type are produced by alternative promoter usage. Beta-type isoforms differ from alpha-type isoforms in their N-terminus.
Isoform 1b (identifier: P0DI97-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1a (identifier: Q9CS84-1)

The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform 2a (identifier: Q9CS84-2)

Also known as: Alpha-2B;

The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform 3a (identifier: Q9CS84-3)

Also known as: Alpha-2C;

The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform 4a (identifier: Q9CS84-4)

The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4646 Potential
Chain47 – 468422Neurexin-1-beta
PRO_0000412630

Regions

Transmembrane393 – 41321Helical; Potential
Domain87 – 285199Laminin G-like
Compositional bias53 – 6513His-rich
Compositional bias315 – 3184Poly-Thr
Compositional bias400 – 4034Poly-Ala

Sites

Metal binding1371Calcium By similarity
Metal binding1541Calcium; via carbonyl oxygen By similarity
Metal binding2361Calcium; via carbonyl oxygen By similarity
Metal binding2381Calcium By similarity

Experimental info

Mutagenesis1371D → A: Loss of NLGN1-binding. No effect on CBLN1-binding. Ref.2
Mutagenesis2381N → A: Loss of NLGN1-binding. No effect on CBLN1-binding. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1b [UniParc].

Last modified September 21, 2011. Version 1.
Checksum: F118A9053BAB4E21

FASTA46850,223
        10         20         30         40         50         60 
MYQRMLRCGA DLGSPGGGSG GGAGGRLALI WIVPLTLSGL LGVAWGASSL GAHHIHHFHG 

        70         80         90        100        110        120 
SSKHHSVPIA IYRSPASLRG GHAGTTYIFS KGGGQITYKW PPNDRPSTRA DRLAIGFSTV 

       130        140        150        160        170        180 
QKEAVLVRVD SSSGLGDYLE LHIHQGKIGV KFNVGTDDIA IEESNAIIND GKYHVVRFTR 

       190        200        210        220        230        240 
SGGNATLQVD SWPVIERYPA GNNDNERLAI ARQRIPYRLG RVVDEWLLDK GRQLTIFNSQ 

       250        260        270        280        290        300 
ATIIIGGKEQ GQPFQGQLSG LYYNGLKVLN MAAENDANIA IVGNVRLVGE VPSSMTTEST 

       310        320        330        340        350        360 
ATAMQSEMST SIMETTTTLA TSTARRGKPP TKEPISQTTD DILVASAECP SDDEDIDPCE 

       370        380        390        400        410        420 
PSSGGLANPT RVGGREPYPG SAEVIRESSS TTGMVVGIVA AAALCILILL YAMYKYRNRD 

       430        440        450        460 
EGSYHVDESR NYISNSAQSN GAVVKEKQPS SAKSANKNKK NKDKEYYV 

« Hide

Isoform 1a [UniParc].

See Q9CS84.

Isoform 2a (Alpha-2B) [UniParc].

See Q9CS84.

Isoform 3a (Alpha-2C) [UniParc].

See Q9CS84.

Isoform 4a [UniParc].

See Q9CS84.

References

« Hide 'large scale' references
[1]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Cbln family proteins promote synapse formation by regulating distinct neurexin signaling pathways in various brain regions."
Matsuda K., Yuzaki M.
Eur. J. Neurosci. 33:1447-1461(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CBLN1, MUTAGENESIS OF ASP-137 AND ASN-238.
[3]"The Cbln family of proteins interact with multiple signaling pathways."
Wei P., Pattarini R., Rong Y., Guo H., Bansal P.K., Kusnoor S.V., Deutch A.Y., Parris J., Morgan J.I.
J. Neurochem. 121:717-729(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CBLN1; CBLN2 AND CBLN4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH466537 Genomic DNA. Translation: EDL38657.1.
UniGeneMm.491332.

3D structure databases

ProteinModelPortalP0DI97.
SMRP0DI97. Positions 84-288.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP0DI97. 2 interactions.

Proteomic databases

PRIDEP0DI97.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000172466; ENSMUSP00000134402; ENSMUSG00000024109. [P0DI97-1]

Organism-specific databases

MGIMGI:1096391. Nrxn1.

Phylogenomic databases

GeneTreeENSGT00750000117263.
TreeFamTF334142.

Gene expression databases

BgeeP0DI97.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
IPR027158. NRXN1_fam.
IPR027789. Syndecan/Neurexin_dom.
[Graphical view]
PANTHERPTHR10127:SF309. PTHR10127:SF309. 1 hit.
PfamPF02210. Laminin_G_2. 1 hit.
PF01034. Syndecan. 1 hit.
[Graphical view]
SMARTSM00294. 4.1m. 1 hit.
SM00282. LamG. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 2 hits.
PROSITEPS50025. LAM_G_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNRXN1. mouse.
PROP0DI97.
SOURCESearch...

Entry information

Entry nameNRX1B_MOUSE
AccessionPrimary (citable) accession number: P0DI97
Entry history
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: April 16, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot