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P0DI97

- NRX1B_MOUSE

UniProt

P0DI97 - NRX1B_MOUSE

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Protein

Neurexin-1-beta

Gene
Nrxn1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Neuronal cell surface protein that may be involved in cell recognition and cell adhesion by forming intracellular junctions through binding to neuroligins. May play a role in formation or maintenance of synaptic junctions. May mediate intracellular signaling. May play a role in angiogenesis By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi137 – 1371Calcium By similarity
Metal bindingi154 – 1541Calcium; via carbonyl oxygen By similarity
Metal bindingi236 – 2361Calcium; via carbonyl oxygen By similarity
Metal bindingi238 – 2381Calcium By similarity

GO - Molecular functioni

  1. calcium-dependent protein binding Source: BHF-UCL
  2. cell adhesion molecule binding Source: BHF-UCL
  3. metal ion binding Source: UniProtKB-KW
  4. neuroligin family protein binding Source: BHF-UCL
  5. protein binding Source: IntAct

GO - Biological processi

  1. angiogenesis Source: UniProtKB-KW
  2. calcium-dependent cell-cell adhesion Source: BHF-UCL
  3. cerebellar granule cell differentiation Source: BHF-UCL
  4. establishment of protein localization Source: BHF-UCL
  5. gamma-aminobutyric acid receptor clustering Source: BHF-UCL
  6. gephyrin clustering Source: BHF-UCL
  7. guanylate kinase-associated protein clustering Source: BHF-UCL
  8. heterophilic cell-cell adhesion Source: BHF-UCL
  9. negative regulation of filopodium assembly Source: BHF-UCL
  10. neuroligin clustering Source: BHF-UCL
  11. neuron cell-cell adhesion Source: BHF-UCL
  12. neuron maturation Source: BHF-UCL
  13. N-methyl-D-aspartate receptor clustering Source: BHF-UCL
  14. positive regulation of establishment of protein localization to plasma membrane Source: BHF-UCL
  15. positive regulation of excitatory postsynaptic membrane potential Source: BHF-UCL
  16. positive regulation of synaptic transmission, glutamatergic Source: BHF-UCL
  17. postsynaptic density protein 95 clustering Source: BHF-UCL
  18. postsynaptic membrane assembly Source: BHF-UCL
  19. presynaptic membrane assembly Source: BHF-UCL
  20. protein localization to synapse Source: BHF-UCL
  21. receptor localization to synapse Source: BHF-UCL
  22. regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
  23. regulation of N-methyl-D-aspartate selective glutamate receptor activity Source: BHF-UCL
  24. synaptic vesicle clustering Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Angiogenesis, Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196607. Non-integrin membrane-ECM interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurexin-1-beta
Alternative name(s):
Neurexin I-beta
Gene namesi
Name:Nrxn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1096391. Nrxn1.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein. Cell junctionsynapse
Note: Localized on the pre-synaptic membrane By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei393 – 41321Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. axonal growth cone Source: BHF-UCL
  2. cell junction Source: UniProtKB-KW
  3. cell surface Source: BHF-UCL
  4. endocytic vesicle Source: BHF-UCL
  5. excitatory synapse Source: BHF-UCL
  6. inhibitory synapse Source: BHF-UCL
  7. integral component of membrane Source: UniProtKB-KW
  8. plasma membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi137 – 1371D → A: Loss of NLGN1-binding. No effect on CBLN1-binding. 1 Publication
Mutagenesisi238 – 2381N → A: Loss of NLGN1-binding. No effect on CBLN1-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4646 Reviewed predictionAdd
BLAST
Chaini47 – 468422Neurexin-1-betaPRO_0000412630Add
BLAST

Post-translational modificationi

Highly O-glycosylated and minor N-glycosylated By similarity.

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP0DI97.

Expressioni

Gene expression databases

BgeeiP0DI97.

Interactioni

Subunit structurei

The cytoplasmic C-terminal region binds to CASK. Isoforms 3b and 4b bind neuroligins NLGN1, NLGN2 and NLGN3, alpha-dystroglycan and alpha-latrotoxin. Binding to neuroligins is calcium-dependent, and the binding preference ranks as follow: NLGN1 > NLGN4 >> NLGN3 > NLGN2 By similarity. Interacts with CBLN1, CBLN2 and more weakly with CBLN4. CBLN1-binding is calcium-independent.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cbln1Q9R1714EBI-2794440,EBI-2794140

Protein-protein interaction databases

IntActiP0DI97. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP0DI97.
SMRiP0DI97. Positions 84-288.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 285199Laminin G-likeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi53 – 6513His-richAdd
BLAST
Compositional biasi315 – 3184Poly-Thr
Compositional biasi400 – 4034Poly-Ala

Sequence similaritiesi

Belongs to the neurexin family.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00750000117263.
OMAiIWIVPLT.
TreeFamiTF334142.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
IPR027789. Syndecan/Neurexin_dom.
[Graphical view]
PfamiPF02210. Laminin_G_2. 1 hit.
PF01034. Syndecan. 1 hit.
[Graphical view]
SMARTiSM00294. 4.1m. 1 hit.
SM00282. LamG. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiPS50025. LAM_G_DOMAIN. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative promoter usage and alternative splicing. Align

Note: A number of isoforms, alpha-type and beta-type are produced by alternative promoter usage. Beta-type isoforms differ from alpha-type isoforms in their N-terminus.

Isoform 1b (identifier: P0DI97-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MYQRMLRCGA DLGSPGGGSG GGAGGRLALI WIVPLTLSGL LGVAWGASSL    50
GAHHIHHFHG SSKHHSVPIA IYRSPASLRG GHAGTTYIFS KGGGQITYKW 100
PPNDRPSTRA DRLAIGFSTV QKEAVLVRVD SSSGLGDYLE LHIHQGKIGV 150
KFNVGTDDIA IEESNAIIND GKYHVVRFTR SGGNATLQVD SWPVIERYPA 200
GNNDNERLAI ARQRIPYRLG RVVDEWLLDK GRQLTIFNSQ ATIIIGGKEQ 250
GQPFQGQLSG LYYNGLKVLN MAAENDANIA IVGNVRLVGE VPSSMTTEST 300
ATAMQSEMST SIMETTTTLA TSTARRGKPP TKEPISQTTD DILVASAECP 350
SDDEDIDPCE PSSGGLANPT RVGGREPYPG SAEVIRESSS TTGMVVGIVA 400
AAALCILILL YAMYKYRNRD EGSYHVDESR NYISNSAQSN GAVVKEKQPS 450
SAKSANKNKK NKDKEYYV 468
Length:468
Mass (Da):50,223
Last modified:September 21, 2011 - v1
Checksum:iF118A9053BAB4E21
GO
Isoform 1a (identifier: Q9CS84-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,514
Mass (Da):166,169
GO
Isoform 2a (identifier: Q9CS84-2) [UniParc]FASTAAdd to Basket

Also known as: Alpha-2B

The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,507
Mass (Da):165,387
GO
Isoform 3a (identifier: Q9CS84-3) [UniParc]FASTAAdd to Basket

Also known as: Alpha-2C

The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,499
Mass (Da):164,596
GO
Isoform 4a (identifier: Q9CS84-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Length:1,176
Mass (Da):129,340
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH466537 Genomic DNA. Translation: EDL38657.1.
UniGeneiMm.491332.

Genome annotation databases

EnsembliENSMUST00000172466; ENSMUSP00000134402; ENSMUSG00000024109. [P0DI97-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH466537 Genomic DNA. Translation: EDL38657.1 .
UniGenei Mm.491332.

3D structure databases

ProteinModelPortali P0DI97.
SMRi P0DI97. Positions 84-288.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P0DI97. 2 interactions.

Proteomic databases

PRIDEi P0DI97.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000172466 ; ENSMUSP00000134402 ; ENSMUSG00000024109 . [P0DI97-1 ]

Organism-specific databases

MGIi MGI:1096391. Nrxn1.

Phylogenomic databases

GeneTreei ENSGT00750000117263.
OMAi IWIVPLT.
TreeFami TF334142.

Enzyme and pathway databases

Reactomei REACT_196607. Non-integrin membrane-ECM interactions.

Miscellaneous databases

ChiTaRSi NRXN1. mouse.
PROi P0DI97.
SOURCEi Search...

Gene expression databases

Bgeei P0DI97.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
IPR027789. Syndecan/Neurexin_dom.
[Graphical view ]
Pfami PF02210. Laminin_G_2. 1 hit.
PF01034. Syndecan. 1 hit.
[Graphical view ]
SMARTi SM00294. 4.1m. 1 hit.
SM00282. LamG. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 2 hits.
PROSITEi PS50025. LAM_G_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Cbln family proteins promote synapse formation by regulating distinct neurexin signaling pathways in various brain regions."
    Matsuda K., Yuzaki M.
    Eur. J. Neurosci. 33:1447-1461(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CBLN1, MUTAGENESIS OF ASP-137 AND ASN-238.
  3. "The Cbln family of proteins interact with multiple signaling pathways."
    Wei P., Pattarini R., Rong Y., Guo H., Bansal P.K., Kusnoor S.V., Deutch A.Y., Parris J., Morgan J.I.
    J. Neurochem. 121:717-729(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CBLN1; CBLN2 AND CBLN4.

Entry informationi

Entry nameiNRX1B_MOUSE
AccessioniPrimary (citable) accession number: P0DI97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: September 3, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi