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P0DI97

- NRX1B_MOUSE

UniProt

P0DI97 - NRX1B_MOUSE

Protein

Neurexin-1-beta

Gene

Nrxn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Neuronal cell surface protein that may be involved in cell recognition and cell adhesion by forming intracellular junctions through binding to neuroligins. May play a role in formation or maintenance of synaptic junctions. May mediate intracellular signaling. May play a role in angiogenesis By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi137 – 1371CalciumBy similarity
    Metal bindingi154 – 1541Calcium; via carbonyl oxygenBy similarity
    Metal bindingi236 – 2361Calcium; via carbonyl oxygenBy similarity
    Metal bindingi238 – 2381CalciumBy similarity

    GO - Molecular functioni

    1. calcium-dependent protein binding Source: BHF-UCL
    2. cell adhesion molecule binding Source: BHF-UCL
    3. metal ion binding Source: UniProtKB-KW
    4. neuroligin family protein binding Source: BHF-UCL
    5. protein binding Source: IntAct

    GO - Biological processi

    1. angiogenesis Source: UniProtKB-KW
    2. calcium-dependent cell-cell adhesion Source: BHF-UCL
    3. cerebellar granule cell differentiation Source: BHF-UCL
    4. establishment of protein localization Source: BHF-UCL
    5. gamma-aminobutyric acid receptor clustering Source: BHF-UCL
    6. gephyrin clustering Source: BHF-UCL
    7. guanylate kinase-associated protein clustering Source: BHF-UCL
    8. heterophilic cell-cell adhesion Source: BHF-UCL
    9. negative regulation of filopodium assembly Source: BHF-UCL
    10. neuroligin clustering Source: BHF-UCL
    11. neuron cell-cell adhesion Source: BHF-UCL
    12. neuron maturation Source: BHF-UCL
    13. N-methyl-D-aspartate receptor clustering Source: BHF-UCL
    14. positive regulation of establishment of protein localization to plasma membrane Source: BHF-UCL
    15. positive regulation of excitatory postsynaptic membrane potential Source: BHF-UCL
    16. positive regulation of synaptic transmission, glutamatergic Source: BHF-UCL
    17. postsynaptic density protein 95 clustering Source: BHF-UCL
    18. postsynaptic membrane assembly Source: BHF-UCL
    19. presynaptic membrane assembly Source: BHF-UCL
    20. protein localization to synapse Source: BHF-UCL
    21. receptor localization to synapse Source: BHF-UCL
    22. regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
    23. regulation of N-methyl-D-aspartate selective glutamate receptor activity Source: BHF-UCL
    24. synaptic vesicle clustering Source: BHF-UCL

    Keywords - Biological processi

    Angiogenesis, Cell adhesion

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196607. Non-integrin membrane-ECM interactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neurexin-1-beta
    Alternative name(s):
    Neurexin I-beta
    Gene namesi
    Name:Nrxn1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1096391. Nrxn1.

    Subcellular locationi

    Cell membrane; Single-pass type I membrane protein. Cell junctionsynapse
    Note: Localized on the pre-synaptic membrane.By similarity

    GO - Cellular componenti

    1. axonal growth cone Source: BHF-UCL
    2. cell junction Source: UniProtKB-KW
    3. cell surface Source: BHF-UCL
    4. endocytic vesicle Source: BHF-UCL
    5. excitatory synapse Source: BHF-UCL
    6. inhibitory synapse Source: BHF-UCL
    7. integral component of membrane Source: UniProtKB-KW
    8. plasma membrane Source: BHF-UCL

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Synapse

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi137 – 1371D → A: Loss of NLGN1-binding. No effect on CBLN1-binding. 1 Publication
    Mutagenesisi238 – 2381N → A: Loss of NLGN1-binding. No effect on CBLN1-binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4646Sequence AnalysisAdd
    BLAST
    Chaini47 – 468422Neurexin-1-betaPRO_0000412630Add
    BLAST

    Post-translational modificationi

    Highly O-glycosylated and minor N-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP0DI97.

    Expressioni

    Gene expression databases

    BgeeiP0DI97.

    Interactioni

    Subunit structurei

    The cytoplasmic C-terminal region binds to CASK. Isoforms 3b and 4b bind neuroligins NLGN1, NLGN2 and NLGN3, alpha-dystroglycan and alpha-latrotoxin. Binding to neuroligins is calcium-dependent, and the binding preference ranks as follow: NLGN1 > NLGN4 >> NLGN3 > NLGN2 By similarity. Interacts with CBLN1, CBLN2 and more weakly with CBLN4. CBLN1-binding is calcium-independent.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cbln1Q9R1714EBI-2794440,EBI-2794140

    Protein-protein interaction databases

    IntActiP0DI97. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP0DI97.
    SMRiP0DI97. Positions 84-288.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei393 – 41321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini87 – 285199Laminin G-likePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi53 – 6513His-richAdd
    BLAST
    Compositional biasi315 – 3184Poly-Thr
    Compositional biasi400 – 4034Poly-Ala

    Sequence similaritiesi

    Belongs to the neurexin family.Curated
    Contains 1 laminin G-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00750000117263.
    OMAiIWIVPLT.
    TreeFamiTF334142.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR001791. Laminin_G.
    IPR003585. Neurexin-like.
    IPR027789. Syndecan/Neurexin_dom.
    [Graphical view]
    PfamiPF02210. Laminin_G_2. 1 hit.
    PF01034. Syndecan. 1 hit.
    [Graphical view]
    SMARTiSM00294. 4.1m. 1 hit.
    SM00282. LamG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 2 hits.
    PROSITEiPS50025. LAM_G_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 5 isoformsi produced by alternative promoter usage and alternative splicing. Align

    Note: A number of isoforms, alpha-type and beta-type are produced by alternative promoter usage. Beta-type isoforms differ from alpha-type isoforms in their N-terminus.

    Isoform 1b (identifier: P0DI97-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MYQRMLRCGA DLGSPGGGSG GGAGGRLALI WIVPLTLSGL LGVAWGASSL    50
    GAHHIHHFHG SSKHHSVPIA IYRSPASLRG GHAGTTYIFS KGGGQITYKW 100
    PPNDRPSTRA DRLAIGFSTV QKEAVLVRVD SSSGLGDYLE LHIHQGKIGV 150
    KFNVGTDDIA IEESNAIIND GKYHVVRFTR SGGNATLQVD SWPVIERYPA 200
    GNNDNERLAI ARQRIPYRLG RVVDEWLLDK GRQLTIFNSQ ATIIIGGKEQ 250
    GQPFQGQLSG LYYNGLKVLN MAAENDANIA IVGNVRLVGE VPSSMTTEST 300
    ATAMQSEMST SIMETTTTLA TSTARRGKPP TKEPISQTTD DILVASAECP 350
    SDDEDIDPCE PSSGGLANPT RVGGREPYPG SAEVIRESSS TTGMVVGIVA 400
    AAALCILILL YAMYKYRNRD EGSYHVDESR NYISNSAQSN GAVVKEKQPS 450
    SAKSANKNKK NKDKEYYV 468
    Length:468
    Mass (Da):50,223
    Last modified:September 21, 2011 - v1
    Checksum:iF118A9053BAB4E21
    GO
    Isoform 1a (identifier: Q9CS84-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry Q9CS84.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:1,514
    Mass (Da):166,169
    GO
    Isoform 2a (identifier: Q9CS84-2) [UniParc]FASTAAdd to Basket

    Also known as: Alpha-2B

    The sequence of this isoform can be found in the external entry Q9CS84.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:1,507
    Mass (Da):165,387
    GO
    Isoform 3a (identifier: Q9CS84-3) [UniParc]FASTAAdd to Basket

    Also known as: Alpha-2C

    The sequence of this isoform can be found in the external entry Q9CS84.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:1,499
    Mass (Da):164,596
    GO
    Isoform 4a (identifier: Q9CS84-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry Q9CS84.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: No experimental confirmation available.

    Length:1,176
    Mass (Da):129,340
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH466537 Genomic DNA. Translation: EDL38657.1.
    UniGeneiMm.491332.

    Genome annotation databases

    EnsembliENSMUST00000172466; ENSMUSP00000134402; ENSMUSG00000024109. [P0DI97-1]

    Keywords - Coding sequence diversityi

    Alternative promoter usage, Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH466537 Genomic DNA. Translation: EDL38657.1 .
    UniGenei Mm.491332.

    3D structure databases

    ProteinModelPortali P0DI97.
    SMRi P0DI97. Positions 84-288.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0DI97. 2 interactions.

    Proteomic databases

    PRIDEi P0DI97.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000172466 ; ENSMUSP00000134402 ; ENSMUSG00000024109 . [P0DI97-1 ]

    Organism-specific databases

    MGIi MGI:1096391. Nrxn1.

    Phylogenomic databases

    GeneTreei ENSGT00750000117263.
    OMAi IWIVPLT.
    TreeFami TF334142.

    Enzyme and pathway databases

    Reactomei REACT_196607. Non-integrin membrane-ECM interactions.

    Miscellaneous databases

    ChiTaRSi NRXN1. mouse.
    PROi P0DI97.
    SOURCEi Search...

    Gene expression databases

    Bgeei P0DI97.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR001791. Laminin_G.
    IPR003585. Neurexin-like.
    IPR027789. Syndecan/Neurexin_dom.
    [Graphical view ]
    Pfami PF02210. Laminin_G_2. 1 hit.
    PF01034. Syndecan. 1 hit.
    [Graphical view ]
    SMARTi SM00294. 4.1m. 1 hit.
    SM00282. LamG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 2 hits.
    PROSITEi PS50025. LAM_G_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Cbln family proteins promote synapse formation by regulating distinct neurexin signaling pathways in various brain regions."
      Matsuda K., Yuzaki M.
      Eur. J. Neurosci. 33:1447-1461(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CBLN1, MUTAGENESIS OF ASP-137 AND ASN-238.
    3. "The Cbln family of proteins interact with multiple signaling pathways."
      Wei P., Pattarini R., Rong Y., Guo H., Bansal P.K., Kusnoor S.V., Deutch A.Y., Parris J., Morgan J.I.
      J. Neurochem. 121:717-729(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CBLN1; CBLN2 AND CBLN4.

    Entry informationi

    Entry nameiNRX1B_MOUSE
    AccessioniPrimary (citable) accession number: P0DI97
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 21, 2011
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3