Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neurexin-1-beta

Gene

Nrxn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuronal cell surface protein that may be involved in cell recognition and cell adhesion by forming intracellular junctions through binding to neuroligins. May play a role in formation or maintenance of synaptic junctions. May mediate intracellular signaling. May play a role in angiogenesis (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi137CalciumBy similarity1
Metal bindingi154Calcium; via carbonyl oxygenBy similarity1
Metal bindingi236Calcium; via carbonyl oxygenBy similarity1
Metal bindingi238CalciumBy similarity1

GO - Molecular functioni

  • acetylcholine receptor binding Source: MGI
  • calcium channel regulator activity Source: MGI
  • calcium-dependent protein binding Source: BHF-UCL
  • cell adhesion molecule binding Source: BHF-UCL
  • metal ion binding Source: UniProtKB-KW
  • neuroligin family protein binding Source: BHF-UCL
  • transmembrane signaling receptor activity Source: BHF-UCL

GO - Biological processi

  • adult behavior Source: MGI
  • angiogenesis Source: UniProtKB-KW
  • calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: BHF-UCL
  • cerebellar granule cell differentiation Source: BHF-UCL
  • chemical synaptic transmission Source: MGI
  • establishment of protein localization Source: BHF-UCL
  • filopodium assembly Source: CACAO
  • gamma-aminobutyric acid receptor clustering Source: BHF-UCL
  • gephyrin clustering involved in postsynaptic density assembly Source: BHF-UCL
  • guanylate kinase-associated protein clustering Source: BHF-UCL
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: BHF-UCL
  • learning Source: MGI
  • negative regulation of filopodium assembly Source: BHF-UCL
  • neuroligin clustering involved in postsynaptic membrane assembly Source: BHF-UCL
  • neuron cell-cell adhesion Source: BHF-UCL
  • neuron maturation Source: BHF-UCL
  • neurotransmitter secretion Source: MGI
  • NMDA glutamate receptor clustering Source: BHF-UCL
  • positive regulation of excitatory postsynaptic potential Source: BHF-UCL
  • positive regulation of protein localization to plasma membrane Source: BHF-UCL
  • positive regulation of synapse assembly Source: MGI
  • positive regulation of synapse maturation Source: MGI
  • positive regulation of synaptic transmission, glutamatergic Source: BHF-UCL
  • postsynaptic density protein 95 clustering Source: BHF-UCL
  • postsynaptic membrane assembly Source: BHF-UCL
  • presynaptic membrane assembly Source: BHF-UCL
  • protein complex assembly involved in synapse maturation Source: BHF-UCL
  • protein localization to synapse Source: BHF-UCL
  • receptor localization to synapse Source: BHF-UCL
  • regulation of AMPA receptor activity Source: BHF-UCL
  • regulation of insulin secretion involved in cellular response to glucose stimulus Source: MGI
  • regulation of NMDA receptor activity Source: BHF-UCL
  • signal transduction Source: BHF-UCL
  • social behavior Source: MGI
  • synapse assembly Source: MGI
  • synaptic vesicle clustering Source: BHF-UCL
  • vesicle docking involved in exocytosis Source: MGI
  • vocalization behavior Source: MGI
  • vocal learning Source: MGI

Keywordsi

Biological processAngiogenesis, Cell adhesion
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-6794361. Neurexins and neuroligins.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurexin-1-beta
Alternative name(s):
Neurexin I-beta
Gene namesi
Name:Nrxn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1096391. Nrxn1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi137D → A: Loss of NLGN1-binding. No effect on CBLN1-binding. 1 Publication1
Mutagenesisi238N → A: Loss of NLGN1-binding. No effect on CBLN1-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 46Sequence analysisAdd BLAST46
ChainiPRO_000041263047 – 468Neurexin-1-betaAdd BLAST422

Post-translational modificationi

Highly O-glycosylated and minor N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP0DI97.
PeptideAtlasiP0DI97.
PRIDEiP0DI97.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024109.
ExpressionAtlasiP0DI97. baseline and differential.
GenevisibleiP0DI97. MM.

Interactioni

Subunit structurei

The cytoplasmic C-terminal region binds to CASK. Binds NLGN1, NLGN2 and NLGN3, DAG1 (alpha-dystroglycan) and alpha-latrotoxin. Binding to neuroligins is calcium-dependent, and the binding preference ranks as follow: NLGN1 > NLGN4 >> NLGN3 > NLGN2 (By similarity). Interacts with CBLN2 and more weakly with CBLN4 (PubMed:22220752). Interacts with CBLN1; interaction is CBLN1 hexamer form-dependent; CBLN1-binding is calcium-independent; isoform 1b does not interact with CBLN1 (PubMed:21410790, PubMed:22220752).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cbln1Q9R1714EBI-2794440,EBI-2794140

GO - Molecular functioni

  • acetylcholine receptor binding Source: MGI
  • calcium-dependent protein binding Source: BHF-UCL
  • cell adhesion molecule binding Source: BHF-UCL
  • neuroligin family protein binding Source: BHF-UCL

Protein-protein interaction databases

IntActiP0DI97. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliP0DI97.
SMRiP0DI97.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 285Laminin G-likePROSITE-ProRule annotationAdd BLAST199

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni201 – 230Essential for interaction with CBLN1; modulates interaction affinity with NLGN1, NLGN2 and NLGN3; prevents interaction with DAG1/alpha-dystroglycan; modulates interaction with alpha-latrotoxinBy similarityAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi53 – 65His-richAdd BLAST13
Compositional biasi315 – 318Poly-Thr4
Compositional biasi400 – 403Poly-Ala4

Sequence similaritiesi

Belongs to the neurexin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3514. Eukaryota.
ENOG410XNU6. LUCA.
GeneTreeiENSGT00760000118991.
KOiK07377.
TreeFamiTF334142.

Family and domain databases

InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR001791. Laminin_G.
IPR003585. Neurexin-like.
IPR027789. Syndecan/Neurexin_dom.
PfamiView protein in Pfam
PF02210. Laminin_G_2. 1 hit.
PF01034. Syndecan. 1 hit.
SMARTiView protein in SMART
SM00294. 4.1m. 1 hit.
SM00282. LamG. 1 hit.
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiView protein in PROSITE
PS50025. LAM_G_DOMAIN. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Note: A number of isoforms, alpha-type and beta-type are produced by alternative promoter usage. Beta-type isoforms differ from alpha-type isoforms in their N-terminus.
Isoform 1b (identifier: P0DI97-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYQRMLRCGA DLGSPGGGSG GGAGGRLALI WIVPLTLSGL LGVAWGASSL
60 70 80 90 100
GAHHIHHFHG SSKHHSVPIA IYRSPASLRG GHAGTTYIFS KGGGQITYKW
110 120 130 140 150
PPNDRPSTRA DRLAIGFSTV QKEAVLVRVD SSSGLGDYLE LHIHQGKIGV
160 170 180 190 200
KFNVGTDDIA IEESNAIIND GKYHVVRFTR SGGNATLQVD SWPVIERYPA
210 220 230 240 250
GNNDNERLAI ARQRIPYRLG RVVDEWLLDK GRQLTIFNSQ ATIIIGGKEQ
260 270 280 290 300
GQPFQGQLSG LYYNGLKVLN MAAENDANIA IVGNVRLVGE VPSSMTTEST
310 320 330 340 350
ATAMQSEMST SIMETTTTLA TSTARRGKPP TKEPISQTTD DILVASAECP
360 370 380 390 400
SDDEDIDPCE PSSGGLANPT RVGGREPYPG SAEVIRESSS TTGMVVGIVA
410 420 430 440 450
AAALCILILL YAMYKYRNRD EGSYHVDESR NYISNSAQSN GAVVKEKQPS
460
SAKSANKNKK NKDKEYYV
Length:468
Mass (Da):50,223
Last modified:September 21, 2011 - v1
Checksum:iF118A9053BAB4E21
GO
Isoform 1a (identifier: Q9CS84-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,514
Mass (Da):166,169
GO
Isoform 2a (identifier: Q9CS84-2) [UniParc]FASTAAdd to basket
Also known as: Alpha-2B
The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,507
Mass (Da):165,387
GO
Isoform 3a (identifier: Q9CS84-3) [UniParc]FASTAAdd to basket
Also known as: Alpha-2C
The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,499
Mass (Da):164,596
GO
Isoform 4a (identifier: Q9CS84-4) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q9CS84.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: No experimental confirmation available.
Length:1,496
Mass (Da):164,369
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH466537 Genomic DNA. Translation: EDL38657.1.
CCDSiCCDS84341.1. [P0DI97-1]
RefSeqiNP_001333888.1. NM_001346959.1. [P0DI97-1]
UniGeneiMm.478418.
Mm.491332.

Genome annotation databases

EnsembliENSMUST00000174337; ENSMUSP00000133724; ENSMUSG00000024109. [P0DI97-1]
GeneIDi18189.
KEGGimmu:18189.

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNRX1B_MOUSE
AccessioniPrimary (citable) accession number: P0DI97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: October 25, 2017
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families