P0DI90 (OXLA2_DABRR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: L-amino-acid oxidase L2 Short name=LAAO Short name=LAAO-L2 Short name=LAO EC=1.4.3.2
Organism	Daboia russelii (Russel's viper) (Vipera russelii)
Taxonomic identifier	31159 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Viperinae › Daboia › Daboia russellii

Protein attributes

Sequence length	20 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, apoptosis of vascular endothelial cells or tumor cell lines, antibacterial and antiparasitic activities, as well as regulation of platelet aggregation. Its effect on platelets is controversial, since it either induces aggregation or inhibits agonist-induced aggregation. These different effects are probably due to different experimental conditions By similarity.
Catalytic activity	An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.
Cofactor	FAD. Ref.1
Subunit structure	Monomer Probable. This is in contrast with most of its orthologs, that are non-covalently linked homodimers. Ref.1
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed by the venom gland. Ref.1
Post-translational modification	N-glycosylated Probable. Ref.1
Sequence similarities	Belongs to the flavin monoamine oxidase family. FIG1 subfamily.
Biophysicochemical properties	Kinetic parameters: KM=1.89 mM for L-Ile Ref.1 KM=599.7 µM for L-Leu KM=222.8 µM for L-Met KM=49.3 µM for L-Phe KM=235.1 µM for L-Trp KM=538.2 µM for L-Tyr Vmax=6.94 µmol/min/mg enzyme toward L-Phe

Ontologies

Keywords
Biological process	Apoptosis Cytolysis Hemolysis
Cellular component	Secreted
Ligand	FAD Flavoprotein
Molecular function	Antibiotic Antimicrobial Hemostasis impairing toxin Oxidoreductase Toxin
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-amino-acid oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›20	›20	L-amino-acid oxidase L2		PRO_0000412601
Amino acid modifications
	Disulfide bond	10 ↔ ?		By similarity
Experimental info
	Non-terminal residue	20	1

Sequences

	Sequence		Length	Mass (Da)
	P0DI90 [UniParc]. Last modified September 21, 2011. Version 1. Checksum: 9D194277D99593E7	FASTA	20	2,310
	10 20 ADDKNPLEEC FCEDDDYCEG

References

[1]

"Two L-amino acid oxidase isoenzymes from Russell's viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs." Mandal S., Bhattacharyya D. FEBS J. 275:2078-2095(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INHIBITION BY SUBSTRATE ANALOGS. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

OXLA2_DABRR

Accession

Primary (citable) accession number: P0DI90

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	September 21, 2011
Last modified:	October 3, 2012
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Annotation program

Animal Toxin Annotation Program