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P0DI89 (OXLA_BOTLC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 9. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
L-amino-acid oxidase

Short name=LAAO
Short name=LAO
EC=1.4.3.2
OrganismBothrops leucurus (White-tailed jararaca) (White-tailed lancehead)
Taxonomic identifier157295 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Protein attributes

Sequence length30 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (high activity against L-Met, L-Leu, L-Nle, L-Trp, L-Phe and moderate activity against L-Tyr), thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, antibacterial and antiparasitic activities By similarity. In addition, this protein induces apoptosis. It also interacts with endothelial cells, and inhibits collagen- and ADP-induced platelet aggregation. L-LAAO family effects on platelets are controversial, since it either induces aggregation or inhibits agonist-induced aggregation. These different effects are probably due to different experimental conditions. Ref.1

Catalytic activity

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactor

FAD By similarity.

Subunit structure

Monomer. This is in contrast with most of its orthologs, that are non-covalently linked homodimers. Ref.1

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Post-translational modification

N-glycosylated. Ref.1

Miscellaneous

Has parasiticidal activities against both trypanosomes and leishmania, as a result of enzyme-catalyzed hydrogen peroxide production (Ref.1).

Sequence similarities

Belongs to the flavin monoamine oxidase family. FIG1 subfamily.

Caution

The existence of several isoforms has been reported that may be due to either different composition or different glycosylation or by the synthesis from different genes (Ref.1).

Biophysicochemical properties

pH dependence:

Optimum pH is 7.5-8.8 for L-Leu. Ref.1

Mass spectrometry

Molecular mass is 57000 Da from positions 1 - ?. Determined by MALDI. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›30›30L-amino-acid oxidase
PRO_0000412596

Amino acid modifications

Disulfide bond10 ↔ ? By similarity

Experimental info

Non-terminal residue301

Sequences

Sequence LengthMass (Da)Tools
P0DI89 [UniParc].

Last modified September 21, 2011. Version 1.
Checksum: 37BCDB4946D486E0

FASTA303,507
        10         20         30 
ADDRNPLEEC FRETDYEEFL EIAKNGLSTT 

« Hide

References

[1]"Cytotoxicity and inhibition of platelet aggregation caused by an l-amino acid oxidase from Bothrops leucurus venom."
Naumann G.B., Silva L.F., Silva L., Faria G., Richardson M., Evangelista K., Kohlhoff M., Gontijo C.M., Navdaev A., de Rezende F.F., Eble J.A., Sanchez E.F.
Biochim. Biophys. Acta 1810:683-694(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, GLYCOSYLATION, MASS SPECTROMETRY.
Tissue: Venom.

Cross-references

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameOXLA_BOTLC
AccessionPrimary (citable) accession number: P0DI89
Entry history
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: February 19, 2014
This is version 9 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families