P0DI86 (OXLA_BOTAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: L-amino-acid oxidase Short name=Balt-LAAO-I Short name=LAAO Short name=LAO EC=1.4.3.2
Organism	Bothrops alternatus (Urutu)
Taxonomic identifier	64174 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Crotalinae › Bothrops

Protein attributes

Sequence length	18 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as slight hemorrhage, induction of platelet aggregation, edema in the mouse paw and bactericidal activity against both Gram-positive (S.aureus) and Gram-negative (E.coli) bacteria. May also induce hemolysis, apoptosis of vascular endothelial cells or tumor cell lines, and may have antiparasitic activities. Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions. Ref.1
Catalytic activity	An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.
Cofactor	FAD By similarity.
Subunit structure	Homodimer; non-covalently linked. Ref.1
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed by the venom gland. Ref.1
Post-translational modification	Contains 2 disulfide bonds By similarity. N-glycosylated Probable. The enzymatic activity is not affected by deglycosylation. Ref.1
Sequence similarities	Belongs to the flavin monoamine oxidase family. FIG1 subfamily.
Caution	The existence of several isoforms has been reported (Ref.1) that may be due to either different composition or different glycosylation or by the synthesis from different genes.

Ontologies

Keywords
Biological process	Apoptosis Cytolysis Hemolysis Inflammatory response
Cellular component	Secreted
Ligand	FAD Flavoprotein
Molecular function	Antibiotic Antimicrobial Hemorrhagic toxin Hemostasis impairing toxin Oxidoreductase Platelet aggregation activating toxin Toxin
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-amino-acid oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›18	›18	L-amino-acid oxidase		PRO_0000412593
Experimental info
	Non-terminal residue	18	1

Sequences

	Sequence		Length	Mass (Da)
	P0DI86 [UniParc]. Last modified September 21, 2011. Version 1. Checksum: CA5F483463FD1ADC	FASTA	18	2,195
	10 ADVRNPLEEF RETDYEVL

References

[1]

"Platelet aggregation and antibacterial effects of an L-amino acid oxidase purified from Bothrops alternatus snake venom." Stabeli R.G., Marcussi S., Carlos G.B., Pietro R.C., Selistre-de-Araujo H.S., Giglio J.R., Oliveira E.B., Soares A.M. Bioorg. Med. Chem. 12:2881-2886(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

OXLA_BOTAL

Accession

Primary (citable) accession number: P0DI86

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	September 21, 2011
Last modified:	October 3, 2012
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program

Annotation program

Chordata Protein Annotation Program