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Protein

Histidinol-phosphate aminotransferase 2, chloroplastic

Gene

HISN6B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate.

Cofactori

Pathwayi: L-histidine biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase 1, chloroplastic (HISN1A), ATP phosphoribosyltransferase 2, chloroplastic (HISN1B)
  2. Histidine biosynthesis bifunctional protein hisIE, chloroplastic (HISN2)
  3. Histidine biosynthesis bifunctional protein hisIE, chloroplastic (HISN2)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (At2g36230), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic, 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (HISN3), 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic (AXX17_At2g32880)
  5. Imidazole glycerol phosphate synthase hisHF, chloroplastic (HISN4)
  6. Imidazoleglycerol-phosphate dehydratase 2, chloroplastic (HISN5B), Imidazoleglycerol-phosphate dehydratase (HISN5B), Imidazoleglycerol-phosphate dehydratase (At3g22425), Imidazoleglycerol-phosphate dehydratase (AXX17_At3g24180), Imidazoleglycerol-phosphate dehydratase 1, chloroplastic (HISN5A), Imidazoleglycerol-phosphate dehydratase (At4g14910), Imidazoleglycerol-phosphate dehydratase (HISN5B), Imidazoleglycerol-phosphate dehydratase (AXX17_At4g17260)
  7. Histidinol-phosphate aminotransferase 2, chloroplastic (HISN6B), Histidinol-phosphate aminotransferase 1, chloroplastic (HISN6A)
  8. Bifunctional phosphatase IMPL2, chloroplastic (HISN7)
  9. Histidinol dehydrogenase, chloroplastic (HISN8), Histidinol dehydrogenase, chloroplastic (AXX17_At5g63470)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • histidine biosynthetic process Source: TAIR

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processAmino-acid biosynthesis, Histidine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00031; UER00012.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidinol-phosphate aminotransferase 2, chloroplastic (EC:2.6.1.9)
Alternative name(s):
Gene duplicate 1-A protein
Imidazole acetol-phosphate transaminase
Protein HISTIDINE BIOSYNTHESIS 6B
Gene namesi
Name:HISN6B
Synonyms:GD1-A, HPA2
Ordered Locus Names:At1g71920
ORF Names:F17M19.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G71920.
TAIRilocus:2016099. AT1G71920.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 40ChloroplastCombined sourcesAdd BLAST40
ChainiPRO_000041643641 – 417Histidinol-phosphate aminotransferase 2, chloroplasticAdd BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41N-acetylalanineCombined sources1
Modified residuei277N6-(pyridoxal phosphate)lysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP0DI07.

Expressioni

Gene expression databases

ExpressionAtlasiP0DI07. baseline and differential.
GenevisibleiP0DI07. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0DI07.
SMRiP0DI07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0633. Eukaryota.
COG0079. LUCA.
InParanoidiP0DI07.
KOiK00817.
OrthoDBiEOG09360A8V.
PhylomeDBiP0DI07.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01023. HisC_aminotrans_2. 1 hit.
InterProiView protein in InterPro
IPR004839. Aminotransferase_I/II.
IPR005861. HisP_aminotrans.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_sub2.
PfamiView protein in Pfam
PF00155. Aminotran_1_2. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01141. hisC. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P0DI07-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVINVQGSP SFSIHSSESN LRKSRALKKP FCSIRNRVYC AQSSSAAVDE
60 70 80 90 100
SKNITMGDSF IRPHLRQLAA YQPILPFEVL SAQLGRKPED IVKLDANENP
110 120 130 140 150
YGPPPEVFEA LGNMKFPYVY PDPQSRRLRD ALAQDSGLES EYILVGCGAD
160 170 180 190 200
ELIDLIMRCV LDPGEKIIDC PPTFSMYVFD AAVNGAGVIK VPRNPDFSLN
210 220 230 240 250
VDRIAEVVEL EKPKCIFLTS PNNPDGSIIS EDDLLKILEM PILVVLDEAY
260 270 280 290 300
IEFSGVESRM KWVKKYENLI VLRTFSKRAG LAGLRVGYGA FPLSIIEYLW
310 320 330 340 350
RAKQPYNVSV AGEVAALAAL SNGKYLEDVR DALVRERERL FGLLKEVPFL
360 370 380 390 400
NPYPSYSNFI LCEVTSGMDA KKLKEDLAKM GVMVRHYNSQ ELKGYVRVSA
410
GKPEHTDVLM ECLKQFY
Length:417
Mass (Da):46,635
Last modified:March 21, 2012 - v1
Checksum:i49CF343430F62AA5
GO
Isoform 2 (identifier: P0DI07-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-113: Missing.

Note: Derived from EST data. No experimental confirmation available.Imported
Show »
Length:304
Mass (Da):34,223
Checksum:iC697111039335F0C
GO

Sequence cautioni

The sequence AAG52232 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85384 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85385 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85386 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85387 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85388 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85389 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85390 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85391 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85392 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85393 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85394 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85395 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85396 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85397 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAR85398 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0588471 – 113Missing in isoform 2. Add BLAST113

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC021665 Genomic DNA. Translation: AAG52232.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35252.1.
CP002684 Genomic DNA. Translation: ANM59283.1.
CP002684 Genomic DNA. Translation: ANM59284.1.
CP002684 Genomic DNA. Translation: ANM59285.1.
AY470000 Genomic DNA. Translation: AAR85384.1. Sequence problems.
AY470001 Genomic DNA. Translation: AAR85385.1. Sequence problems.
AY470002 Genomic DNA. Translation: AAR85386.1. Sequence problems.
AY470003 Genomic DNA. Translation: AAR85387.1. Sequence problems.
AY470004 Genomic DNA. Translation: AAR85388.1. Sequence problems.
AY470005 Genomic DNA. Translation: AAR85389.1. Sequence problems.
AY470006 Genomic DNA. Translation: AAR85390.1. Sequence problems.
AY470007 Genomic DNA. Translation: AAR85391.1. Sequence problems.
AY470008 Genomic DNA. Translation: AAR85392.1. Sequence problems.
AY470009 Genomic DNA. Translation: AAR85393.1. Sequence problems.
AY470010 Genomic DNA. Translation: AAR85394.1. Sequence problems.
AY470011 Genomic DNA. Translation: AAR85395.1. Sequence problems.
AY470012 Genomic DNA. Translation: AAR85396.1. Sequence problems.
AY470013 Genomic DNA. Translation: AAR85397.1. Sequence problems.
AY470014 Genomic DNA. Translation: AAR85398.1. Sequence problems.
RefSeqiNP_001031867.1. NM_001036790.2. [P0DI07-1]
NP_001117584.1. NM_001124112.2. [P0DI07-1]
NP_001319364.1. NM_001334509.1. [P0DI07-1]
NP_001321653.1. NM_001334511.1. [P0DI07-2]
NP_001321654.1. NM_001334510.1. [P0DI07-2]
NP_001330915.1. NM_001343108.1. [P0DI07-2]
NP_001330916.1. NM_001343107.1. [P0DI07-2]
NP_001330917.1. NM_001343106.1. [P0DI07-2]
NP_001330918.1. NM_001343105.1. [P0DI07-2]
NP_177337.1. NM_105850.3.
NP_568226.1. NM_121071.4. [P0DI07-1]
UniGeneiAt.49003.
At.66855.

Genome annotation databases

EnsemblPlantsiAT1G71920.2; AT1G71920.2; AT1G71920.
AT1G71920.4; AT1G71920.4; AT1G71920.
AT1G71920.5; AT1G71920.5; AT1G71920.
AT1G71920.6; AT1G71920.6; AT1G71920.
AT5G10330.1; AT5G10330.1; AT5G10330.
AT5G10330.2; AT5G10330.2; AT5G10330.
AT5G10330.4; AT5G10330.4; AT5G10330.
AT5G10330.5; AT5G10330.5; AT5G10330.
AT5G10330.6; AT5G10330.6; AT5G10330.
AT5G10330.7; AT5G10330.7; AT5G10330.
GeneIDi830897.
843523.
GrameneiAT1G71920.2; AT1G71920.2; AT1G71920.
AT1G71920.4; AT1G71920.4; AT1G71920.
AT1G71920.5; AT1G71920.5; AT1G71920.
AT1G71920.6; AT1G71920.6; AT1G71920.
AT5G10330.1; AT5G10330.1; AT5G10330.
AT5G10330.2; AT5G10330.2; AT5G10330.
AT5G10330.4; AT5G10330.4; AT5G10330.
AT5G10330.5; AT5G10330.5; AT5G10330.
AT5G10330.6; AT5G10330.6; AT5G10330.
AT5G10330.7; AT5G10330.7; AT5G10330.
KEGGiath:AT1G71920.
ath:AT5G10330.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHIS6B_ARATH
AccessioniPrimary (citable) accession number: P0DI07
Secondary accession number(s): Q6S4C1
, Q6S4D6, Q949X3, Q9LD56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: March 21, 2012
Last modified: August 30, 2017
This is version 49 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families