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P0DH87 (PSRK_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative inactive G-type lectin S-receptor-like serine/threonine-protein kinase SRK
Alternative name(s):
Pseudogene of S-locus receptor kinase A
Gene names
Name:PSEUDOSRKA
Ordered Locus Names:At4g21370
ORF Names:T6K22.100
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length546 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceUncertain

General annotation (Comments)

Function

Truncated and inactivated form of SRK, the female specificity determinant of self-incompatibility when active. Most A.thaliana cultivars contain such an inactive form and thus, are self-fertiles. Ref.2 Ref.3 Ref.4

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity.

Domain

The protein kinase domain is predicted to be catalytically inactive.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 bulb-type lectin domain.

Contains 1 EGF-like domain.

Contains 1 PAN domain.

Contains 1 protein kinase domain.

Caution

Could be the product of a pseudogene. In strain cv. Columbia, a frameshift mutation introduces a premature stop codon leading to a truncated SRK protein. A complete sequence for SRK can be found in strains cv. Pog-0 and cv. Wei-1 (AC P0DH86).

Sequence caution

The sequence CAA20202.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB79136.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 546515Putative inactive G-type lectin S-receptor-like serine/threonine-protein kinase SRK
PRO_0000413168

Regions

Topological domain32 – 441410Extracellular Potential
Transmembrane442 – 46221Helical; Potential
Topological domain463 – 54684Cytoplasmic Potential
Domain34 – 154121Bulb-type lectin
Domain293 – 32937EGF-like; atypical
Domain348 – 42881PAN
Domain524 – 54623Protein kinase
Nucleotide binding530 – 5389ATP By similarity

Amino acid modifications

Glycosylation461N-linked (GlcNAc...) Potential
Glycosylation1201N-linked (GlcNAc...) Potential
Glycosylation1471N-linked (GlcNAc...) Potential
Glycosylation2431N-linked (GlcNAc...) Potential
Glycosylation3871N-linked (GlcNAc...) Potential
Disulfide bond297 ↔ 309 By similarity
Disulfide bond303 ↔ 317 By similarity
Disulfide bond378 ↔ 403 By similarity
Disulfide bond382 ↔ 388 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0DH87 [UniParc].

Last modified October 19, 2011. Version 1.
Checksum: 7815DBBCAD3878BA

FASTA54662,093
        10         20         30         40         50         60 
MRGELPNKHH SYTFFVFLFF FLILFPDLSI SVNTLSATES LTISSNKTIV SPGGVFELGF 

        70         80         90        100        110        120 
FRILGDSWYL GIWYKKISQR TYVWVANRDT PLSNPIGILK ISNANLVILD NSDTHVWSTN 

       130        140        150        160        170        180 
LTGAVRSSVV AELLDNGNFV LRGSKINESD EFLWQSFDFP TDTLLPQMKL GRDHKRGLNR 

       190        200        210        220        230        240 
FVTSWKSSFD PSSGSFMFKL ETLGLPEFFG FTSFLEVYRS GPWDGLRFSG ILEMQQWDDI 

       250        260        270        280        290        300 
IYNFTENREE VAYTFRVTDH NSYSRLTINT VGRLEGFTWE PTQQEWNMFW FMPKDTCDLY 

       310        320        330        340        350        360 
GICGPYAYCD MSTSPTCNCI KGFQPLSPQD WASGDVTGRC RRKTQLTCGE DRFFRLMNMK 

       370        380        390        400        410        420 
IPATTAAIVD KRIGLKECEE KCKTHCNCTA YANSDIRNGG SGCIIWIGEF RDIRNYAADG 

       430        440        450        460        470        480 
QDLFVRLAAA EFGERRTIRG KIIGLIIGIS LMLVLSFIIY CFWKKKQKRA RATAAPIGYR 

       490        500        510        520        530        540 
DRIQELIITN GVVMSSGRRL LGEEEDLELP LTEFETVVMA TENFSDSNIL GRGGFGIVYK 


GRLLDG 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Natural variation in expression of self-incompatibility in Arabidopsis thaliana: implications for the evolution of selfing."
Nasrallah M.E., Liu P., Sherman-Broyles S., Boggs N.A., Nasrallah J.B.
Proc. Natl. Acad. Sci. U.S.A. 101:16070-16074(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"S locus genes and the evolution of self-fertility in Arabidopsis thaliana."
Sherman-Broyles S., Boggs N., Farkas A., Liu P., Vrebalov J., Nasrallah M.E., Nasrallah J.B.
Plant Cell 19:94-106(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, REVIEW.
[4]"The evolution of selfing in Arabidopsis thaliana."
Tang C., Toomajian C., Sherman-Broyles S., Plagnol V., Guo Y.-L., Hu T.T., Clark R.M., Nasrallah J.B., Weigel D., Nordborg M.
Science 317:1070-1072(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Web resources

PlantP kinase Classification PPC
Protein Spotlight

Do it yourself - Issue 128 of May 2011

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL031187 Genomic DNA. Translation: CAA20202.1. Sequence problems.
AL161554 Genomic DNA. Translation: CAB79136.1. Sequence problems.
PIRT05179.

3D structure databases

ProteinModelPortalP0DH87.
SMRP0DH87. Positions 33-209, 519-546.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP0DH87.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GeneFarm73. 3.
TAIRAT4G21370.

Family and domain databases

Gene3D2.90.10.10. 1 hit.
InterProIPR001480. Bulb-type_lectin_dom.
IPR000742. EG-like_dom.
IPR011009. Kinase-like_dom.
IPR013227. PAN-2_domain.
IPR003609. Pan_app.
IPR022126. S-locus_recpt_kinase.
IPR000858. S_locus_glycoprot.
[Graphical view]
PfamPF01453. B_lectin. 1 hit.
PF12398. DUF3660. 1 hit.
PF08276. PAN_2. 1 hit.
PF00954. S_locus_glycop. 1 hit.
[Graphical view]
SMARTSM00108. B_lectin. 1 hit.
SM00181. EGF. 1 hit.
SM00473. PAN_AP. 1 hit.
[Graphical view]
SUPFAMSSF51110. SSF51110. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS50927. BULB_LECTIN. 1 hit.
PS50948. PAN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSRK_ARATH
AccessionPrimary (citable) accession number: P0DH87
Secondary accession number(s): B0F2A9 expand/collapse secondary AC list , B0F2B0, D6NTN9, D6NTP0, D6NTP1, D6NTP2, D6NTP6, D6NTP7, D6NTP8, O81904
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 19, 2011
Last modified: March 19, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names