P0DH87 (PSRK_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative inactive G-type lectin S-receptor-like serine/threonine-protein kinase SRK

Alternative name(s):
Pseudogene of S-locus receptor kinase A

Gene names

Name:	PSEUDOSRKA
Ordered Locus Names:	At4g21370
ORF Names:	T6K22.100

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Uncertain

General annotation (Comments)

Function	Truncated and inactivated form of SRK, the female specificity determinant of self-incompatibility when active. Most A.thaliana cultivars contain such an inactive form and thus, are self-fertiles. Ref.2 Ref.3 Ref.4
Subcellular location	Cell membrane; Single-pass type I membrane protein By similarity.
Domain	The protein kinase domain is predicted to be catalytically inactive.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 bulb-type lectin domain. Contains 1 EGF-like domain. Contains 1 PAN domain. Contains 1 protein kinase domain.
Caution	Could be the product of a pseudogene. In strain cv. Columbia, a frameshift mutation introduces a premature stop codon leading to a truncated SRK protein. A complete sequence for SRK can be found in strains cv. Pog-0 and cv. Wei-1 (AC P0DH86).
Sequence caution	The sequence CAA20202.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79136.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Lectin Nucleotide-binding
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to bacterium, incompatible interaction Inferred from sequence or structural similarity. Source: UniProtKB protein autophosphorylation Inferred from sequence or structural similarity. Source: UniProtKB recognition of pollen Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane part Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 546	515	Putative inactive G-type lectin S-receptor-like serine/threonine-protein kinase SRK		PRO_0000413168
Regions
	Topological domain	32 – 441	410	Extracellular Potential
	Transmembrane	442 – 462	21	Helical; Potential
	Topological domain	463 – 546	84	Cytoplasmic Potential
	Domain	34 – 154	121	Bulb-type lectin
	Domain	293 – 329	37	EGF-like; atypical
	Domain	348 – 428	81	PAN
	Domain	524 – 546	23	Protein kinase
	Nucleotide binding	530 – 538	9	ATP By similarity
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	120	1	N-linked (GlcNAc...) Potential
	Glycosylation	147	1	N-linked (GlcNAc...) Potential
	Glycosylation	243	1	N-linked (GlcNAc...) Potential
	Glycosylation	387	1	N-linked (GlcNAc...) Potential
	Disulfide bond	297 ↔ 309		By similarity
	Disulfide bond	303 ↔ 317		By similarity
	Disulfide bond	378 ↔ 403		By similarity
	Disulfide bond	382 ↔ 388		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0DH87 [UniParc]. Last modified October 19, 2011. Version 1. Checksum: 7815DBBCAD3878BA	FASTA	546	62,093
	10 20 30 40 50 60 MRGELPNKHH SYTFFVFLFF FLILFPDLSI SVNTLSATES LTISSNKTIV SPGGVFELGF 70 80 90 100 110 120 FRILGDSWYL GIWYKKISQR TYVWVANRDT PLSNPIGILK ISNANLVILD NSDTHVWSTN 130 140 150 160 170 180 LTGAVRSSVV AELLDNGNFV LRGSKINESD EFLWQSFDFP TDTLLPQMKL GRDHKRGLNR 190 200 210 220 230 240 FVTSWKSSFD PSSGSFMFKL ETLGLPEFFG FTSFLEVYRS GPWDGLRFSG ILEMQQWDDI 250 260 270 280 290 300 IYNFTENREE VAYTFRVTDH NSYSRLTINT VGRLEGFTWE PTQQEWNMFW FMPKDTCDLY 310 320 330 340 350 360 GICGPYAYCD MSTSPTCNCI KGFQPLSPQD WASGDVTGRC RRKTQLTCGE DRFFRLMNMK 370 380 390 400 410 420 IPATTAAIVD KRIGLKECEE KCKTHCNCTA YANSDIRNGG SGCIIWIGEF RDIRNYAADG 430 440 450 460 470 480 QDLFVRLAAA EFGERRTIRG KIIGLIIGIS LMLVLSFIIY CFWKKKQKRA RATAAPIGYR 490 500 510 520 530 540 DRIQELIITN GVVMSSGRRL LGEEEDLELP LTEFETVVMA TENFSDSNIL GRGGFGIVYK GRLLDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Natural variation in expression of self-incompatibility in Arabidopsis thaliana: implications for the evolution of selfing." Nasrallah M.E., Liu P., Sherman-Broyles S., Boggs N.A., Nasrallah J.B. Proc. Natl. Acad. Sci. U.S.A. 101:16070-16074(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"S locus genes and the evolution of self-fertility in Arabidopsis thaliana." Sherman-Broyles S., Boggs N., Farkas A., Liu P., Vrebalov J., Nasrallah M.E., Nasrallah J.B. Plant Cell 19:94-106(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, REVIEW.
[4]	"The evolution of selfing in Arabidopsis thaliana." Tang C., Toomajian C., Sherman-Broyles S., Plagnol V., Guo Y.-L., Hu T.T., Clark R.M., Nasrallah J.B., Weigel D., Nordborg M. Science 317:1070-1072(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Web resources

PlantP kinase Classification PPC

Protein Spotlight

Do it yourself - Issue 128 of May 2011

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL031187 Genomic DNA. Translation: CAA20202.1. Sequence problems. AL161554 Genomic DNA. Translation: CAB79136.1. Sequence problems.
PIR	T05179.
3D structure databases
ProteinModelPortal	P0DH87.
SMR	P0DH87. Positions 519-546.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
GeneFarm	73. 3.
TAIR	At4g21370.
Family and domain databases
Gene3D	2.90.10.10. 1 hit.
InterPro	IPR001480. Bulb-type_lectin_dom. IPR000742. EG-like_dom. IPR011009. Kinase-like_dom. IPR013227. PAN-2_domain. IPR003609. Pan_app. IPR022126. S-locus_recpt_kinase. IPR000858. S_locus_glycoprot. [Graphical view]
Pfam	PF01453. B_lectin. 1 hit. PF12398. DUF3660. 1 hit. PF08276. PAN_2. 1 hit. PF00954. S_locus_glycop. 1 hit. [Graphical view]
SMART	SM00108. B_lectin. 1 hit. SM00181. EGF. 1 hit. SM00473. PAN_AP. 1 hit. [Graphical view]
SUPFAM	SSF51110. B_lectin. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS50927. BULB_LECTIN. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. False negative. PS50026. EGF_3. False negative. PS50948. PAN. 1 hit. PS50011. PROTEIN_KINASE_DOM. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSRK_ARATH

Accession

Primary (citable) accession number: P0DH87
Secondary accession number(s): B0F2A9 , B0F2B0, D6NTN9, D6NTP0, D6NTP1, D6NTP2, D6NTP6, D6NTP7, D6NTP8, O81904

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	October 19, 2011
Last modified:	May 1, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program