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P0DH86

- SRK_ARATH

UniProt

P0DH86 - SRK_ARATH

Protein

G-type lectin S-receptor-like serine/threonine-protein kinase SRK

Gene

SRK

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 20 (01 Oct 2014)
      Sequence version 1 (19 Oct 2011)
      Previous versions | rss
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    Functioni

    Female specificity determinant of self-incompatibility.4 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei552 – 5521ATPPROSITE-ProRule annotation
    Active sitei650 – 6501Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi530 – 5389ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calmodulin binding Source: UniProtKB
    3. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. defense response to bacterium, incompatible interaction Source: UniProtKB
    2. protein autophosphorylation Source: UniProtKB
    3. recognition of pollen Source: InterPro

    Keywords - Molecular functioni

    Kinase, Receptor, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Lectin, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G-type lectin S-receptor-like serine/threonine-protein kinase SRK (EC:2.7.11.1)
    Gene namesi
    Name:SRK
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 853822G-type lectin S-receptor-like serine/threonine-protein kinase SRKPRO_0000401305Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi243 – 2431N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi297 ↔ 309By similarity
    Disulfide bondi303 ↔ 317By similarity
    Disulfide bondi378 ↔ 403By similarity
    Disulfide bondi382 ↔ 388By similarity
    Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP0DH86.

    Structurei

    3D structure databases

    ProteinModelPortaliP0DH86.
    SMRiP0DH86. Positions 33-161, 508-804.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini32 – 441410ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini463 – 853391CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei442 – 46221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 154121Bulb-type lectinPROSITE-ProRule annotationAdd
    BLAST
    Domaini293 – 32937EGF-like; atypicalAdd
    BLAST
    Domaini348 – 42881PANPROSITE-ProRule annotationAdd
    BLAST
    Domaini524 – 802279Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni613 – 63119CaM-bindingBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi829 – 8324Poly-Ser

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 bulb-type lectin domain.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.Curated
    Contains 1 PAN domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    2.90.10.10. 1 hit.
    InterProiIPR001480. Bulb-type_lectin_dom.
    IPR013320. ConA-like_subgrp.
    IPR000742. EG-like_dom.
    IPR011009. Kinase-like_dom.
    IPR013227. PAN-2_domain.
    IPR003609. Pan_app.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR022126. S-locus_recpt_kinase.
    IPR021820. S-locus_recpt_kinase_C.
    IPR000858. S_locus_glycoprot.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR024171. SRK_like_kinase.
    [Graphical view]
    PfamiPF01453. B_lectin. 1 hit.
    PF11883. DUF3403. 1 hit.
    PF12398. DUF3660. 1 hit.
    PF08276. PAN_2. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF00954. S_locus_glycop. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000641. SRK. 1 hit.
    SMARTiSM00108. B_lectin. 1 hit.
    SM00181. EGF. 1 hit.
    SM00473. PAN_AP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51110. SSF51110. 2 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS50927. BULB_LECTIN. 1 hit.
    PS50948. PAN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0DH86-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRGELPNKHH SYTFFVFLFF FLILFPDLSI SVNTLSATES LTISSNKTIV    50
    SPGGVFELGF FRILGDSWYL GIWYKKISQR TYVWVANRDT PLSNPIGILK 100
    ISNANLVILD NSDTHVWSTN LTGAVRSSVV AELLDNGNFV LRGSKINESD 150
    EFLWQSFDFP TDTLLPQMKL GRDHKRGLNR FVTSWKSSFD PSSGSFMFKL 200
    ETLGLPEFFG FTSFLEVYRS GPWDGLRFSG ILEMQQWDDI IYNFTENREE 250
    VAYTFRVTDH NSYSRLTINT VGRLEGFMWE PTQQEWNMFW FMPKDTCDLY 300
    GICGPYAYCD MSTSPTCNCI KGFQPLSPQD WASGDVTGRC RRKTQLTCGE 350
    DRFFRLMNMK IPATTAAIVD KRIGLKECEE KCKTHCNCTA YANSDIRNGG 400
    SGCIIWIGEF RDIRNYAADG QDLFVRLAAA EFGERRTIRG KIIGLIIGIS 450
    LMLVLSFIIY CFWKKKQKRA RATAAPIGYR DRIQELIITN GVVMSSGRRL 500
    LGEEEDLELP LTEFETVVMA TENFSDSNIL GRGGFGIVYK GRLLDGQEIA 550
    VKRLSEMSSQ GTNEFKNEVR LIARLQHINL VRLLSCCIYA DEKILIYEYL 600
    ENGSLDSHLF ETTQSSNKLN WQTRFSIING IARGLLYLHQ DSRFKIIHRD 650
    LKASNVLLDK NMTPKISDFG MARIFERDET EANTRKVVGT YGYMSPEYAM 700
    EGIFSVKSDV FSFGVLVLEI VSGKRNRGFH NSGQDNNLLG YTWENWKEGK 750
    GLEIVDSIIV DSSSSMSLFQ PHEVLRCIQI GLLCVQERAE DRPKMSSVVL 800
    MLGSEKGEIP QPKRPGYCVG RSSLDTADSS SSTKRDSESL TVNQITVSVI 850
    NAR 853
    Length:853
    Mass (Da):96,764
    Last modified:October 19, 2011 - v1
    Checksum:i9A74E475EEA40204
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti45 – 451S → N in strain: cv. Ge-1. 1 Publication
    Natural varianti269 – 2691N → Y in strain: cv. Ge-1. 1 Publication
    Natural varianti278 – 2781M → T in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
    Natural varianti373 – 3731I → T in strain: cv. Nok-0. 1 Publication
    Natural varianti415 – 4151N → K in strain: cv. Ca-0 and cv. Co. 1 Publication
    Natural varianti420 – 4201G → A in strain: cv. Fi-1. 1 Publication
    Natural varianti438 – 4381I → S in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
    Natural varianti562 – 5621T → I in strain: cv. Ca-0. 1 Publication
    Natural varianti612 – 6121T → R in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
    Natural varianti630 – 6301G → D in strain: cv. Ca-0. 1 Publication
    Natural varianti676 – 6761E → Q in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
    Natural varianti829 – 8379Missing in strain: cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF692484 Genomic DNA. Translation: ABU54597.1.
    EF692485 Genomic DNA. Translation: ABU54598.1.
    EF692488 mRNA. Translation: ABU54595.1.
    EF692489 mRNA. Translation: ABU54596.1.
    GU723782 mRNA. Translation: ADG01645.1.
    GU723783 mRNA. Translation: ADG01646.1.
    GU723784 mRNA. Translation: ADG01647.1.
    GU723785 mRNA. Translation: ADG01648.1.
    GU723786 mRNA. Translation: ADG01649.1.
    GU723787 mRNA. Translation: ADG01650.1.
    GU723788 mRNA. Translation: ADG01651.1.
    GU723789 mRNA. Translation: ADG01652.1.
    GU723790 mRNA. Translation: ADG01653.1.
    GU723791 mRNA. Translation: ADG01654.1.
    GU723866 Genomic DNA. Translation: ADG01729.1.
    GU723867 Genomic DNA. Translation: ADG01730.1.
    GU723870 Genomic DNA. Translation: ADG01731.1.
    GU723871 Genomic DNA. Translation: ADG01732.1.
    GU723872 Genomic DNA. Translation: ADG01733.1.
    GU723873 Genomic DNA. Translation: ADG01734.1.
    GU723874 Genomic DNA. Translation: ADG01735.1.
    GU723875 Genomic DNA. Translation: ADG01736.1.
    GU723876 Genomic DNA. Translation: ADG01737.1.
    GU723877 Genomic DNA. Translation: ADG01738.1.

    Cross-referencesi

    Web resourcesi

    PlantP kinase Classification PPC
    Protein Spotlight

    Do it yourself - Issue 128 of May 2011

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF692484 Genomic DNA. Translation: ABU54597.1 .
    EF692485 Genomic DNA. Translation: ABU54598.1 .
    EF692488 mRNA. Translation: ABU54595.1 .
    EF692489 mRNA. Translation: ABU54596.1 .
    GU723782 mRNA. Translation: ADG01645.1 .
    GU723783 mRNA. Translation: ADG01646.1 .
    GU723784 mRNA. Translation: ADG01647.1 .
    GU723785 mRNA. Translation: ADG01648.1 .
    GU723786 mRNA. Translation: ADG01649.1 .
    GU723787 mRNA. Translation: ADG01650.1 .
    GU723788 mRNA. Translation: ADG01651.1 .
    GU723789 mRNA. Translation: ADG01652.1 .
    GU723790 mRNA. Translation: ADG01653.1 .
    GU723791 mRNA. Translation: ADG01654.1 .
    GU723866 Genomic DNA. Translation: ADG01729.1 .
    GU723867 Genomic DNA. Translation: ADG01730.1 .
    GU723870 Genomic DNA. Translation: ADG01731.1 .
    GU723871 Genomic DNA. Translation: ADG01732.1 .
    GU723872 Genomic DNA. Translation: ADG01733.1 .
    GU723873 Genomic DNA. Translation: ADG01734.1 .
    GU723874 Genomic DNA. Translation: ADG01735.1 .
    GU723875 Genomic DNA. Translation: ADG01736.1 .
    GU723876 Genomic DNA. Translation: ADG01737.1 .
    GU723877 Genomic DNA. Translation: ADG01738.1 .

    3D structure databases

    ProteinModelPortali P0DH86.
    SMRi P0DH86. Positions 33-161, 508-804.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P0DH86.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    2.90.10.10. 1 hit.
    InterProi IPR001480. Bulb-type_lectin_dom.
    IPR013320. ConA-like_subgrp.
    IPR000742. EG-like_dom.
    IPR011009. Kinase-like_dom.
    IPR013227. PAN-2_domain.
    IPR003609. Pan_app.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR022126. S-locus_recpt_kinase.
    IPR021820. S-locus_recpt_kinase_C.
    IPR000858. S_locus_glycoprot.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR024171. SRK_like_kinase.
    [Graphical view ]
    Pfami PF01453. B_lectin. 1 hit.
    PF11883. DUF3403. 1 hit.
    PF12398. DUF3660. 1 hit.
    PF08276. PAN_2. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF00954. S_locus_glycop. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000641. SRK. 1 hit.
    SMARTi SM00108. B_lectin. 1 hit.
    SM00181. EGF. 1 hit.
    SM00473. PAN_AP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51110. SSF51110. 2 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS50927. BULB_LECTIN. 1 hit.
    PS50948. PAN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Independent origins of self-compatibility in Arabidopsis thaliana."
      Shimizu K.K., Shimizu-Inatsugi R., Tsuchimatsu T., Purugganan M.D.
      Mol. Ecol. 17:704-714(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT THR-373.
      Strain: cv. Nok-0 and cv. Pog-0.
    2. "Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene."
      Tsuchimatsu T., Suwabe K., Shimizu-Inatsugi R., Isokawa S., Pavlidis P., Stadler T., Suzuki G., Takayama S., Watanabe M., Shimizu K.K.
      Nature 464:1342-1346(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS ASN-45; TYR-269; THR-278; LYS-415; ALA-420; SER-438; ILE-562; ARG-612; ASP-630; GLN-676 AND 829-SER--SER-837 DEL, FUNCTION.
      Strain: cv. Ca-0, cv. Co, cv. Di-1, cv. Fi-1, cv. Ge-1, cv. Gie, cv. Old-1, cv. Uk-3, cv. Wassilewskija and cv. Wei-1.
    3. "Natural variation in expression of self-incompatibility in Arabidopsis thaliana: implications for the evolution of selfing."
      Nasrallah M.E., Liu P., Sherman-Broyles S., Boggs N.A., Nasrallah J.B.
      Proc. Natl. Acad. Sci. U.S.A. 101:16070-16074(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "S locus genes and the evolution of self-fertility in Arabidopsis thaliana."
      Sherman-Broyles S., Boggs N., Farkas A., Liu P., Vrebalov J., Nasrallah M.E., Nasrallah J.B.
      Plant Cell 19:94-106(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, REVIEW.
    5. Cited for: FUNCTION.

    Entry informationi

    Entry nameiSRK_ARATH
    AccessioniPrimary (citable) accession number: P0DH86
    Secondary accession number(s): B0F2A9
    , B0F2B0, D6NTN9, D6NTP0, D6NTP1, D6NTP2, D6NTP6, D6NTP7, D6NTP8, O81904
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2011
    Last sequence update: October 19, 2011
    Last modified: October 1, 2014
    This is version 20 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Caution

    Has been shown to be a pseudogene in cv. Columbia (AC P0DH87) due to a frameshift mutation that introduces a premature stop codon in this strain. The sequence shown is from strains cv. Pog-0 and cv. Wei-1.Curated

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3