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Protein

G-type lectin S-receptor-like serine/threonine-protein kinase SRK

Gene

SRK

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Female specificity determinant of self-incompatibility.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei552ATPPROSITE-ProRule annotation1
Active sitei650Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi530 – 538ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Lectin, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
G-type lectin S-receptor-like serine/threonine-protein kinase SRK (EC:2.7.11.1)
Gene namesi
Name:SRK
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 441ExtracellularSequence analysisAdd BLAST410
Transmembranei442 – 462HelicalSequence analysisAdd BLAST21
Topological domaini463 – 853CytoplasmicSequence analysisAdd BLAST391

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000040130532 – 853G-type lectin S-receptor-like serine/threonine-protein kinase SRKAdd BLAST822

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi46N-linked (GlcNAc...)Sequence analysis1
Glycosylationi120N-linked (GlcNAc...)Sequence analysis1
Glycosylationi147N-linked (GlcNAc...)Sequence analysis1
Glycosylationi243N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi297 ↔ 309By similarity
Disulfide bondi303 ↔ 317By similarity
Disulfide bondi378 ↔ 403By similarity
Disulfide bondi382 ↔ 388By similarity
Glycosylationi387N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliP0DH86.
SMRiP0DH86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 154Bulb-type lectinPROSITE-ProRule annotationAdd BLAST121
Domaini293 – 329EGF-like; atypicalAdd BLAST37
Domaini348 – 428PANPROSITE-ProRule annotationAdd BLAST81
Domaini524 – 802Protein kinasePROSITE-ProRule annotationAdd BLAST279

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni613 – 631CaM-bindingBy similarityAdd BLAST19

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi829 – 832Poly-Ser4

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 bulb-type lectin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.Curated
Contains 1 PAN domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
2.90.10.10. 1 hit.
InterProiIPR001480. Bulb-type_lectin_dom.
IPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR003609. Pan_app.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR022126. S-locus_recpt_kinase.
IPR021820. S-locus_recpt_kinase_C.
IPR000858. S_locus_glycoprot_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR024171. SRK-like_kinase.
[Graphical view]
PfamiPF01453. B_lectin. 1 hit.
PF11883. DUF3403. 1 hit.
PF12398. DUF3660. 1 hit.
PF08276. PAN_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00954. S_locus_glycop. 1 hit.
[Graphical view]
PIRSFiPIRSF000641. SRK. 1 hit.
SMARTiSM00108. B_lectin. 1 hit.
SM00473. PAN_AP. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF51110. SSF51110. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50927. BULB_LECTIN. 1 hit.
PS50948. PAN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0DH86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGELPNKHH SYTFFVFLFF FLILFPDLSI SVNTLSATES LTISSNKTIV
60 70 80 90 100
SPGGVFELGF FRILGDSWYL GIWYKKISQR TYVWVANRDT PLSNPIGILK
110 120 130 140 150
ISNANLVILD NSDTHVWSTN LTGAVRSSVV AELLDNGNFV LRGSKINESD
160 170 180 190 200
EFLWQSFDFP TDTLLPQMKL GRDHKRGLNR FVTSWKSSFD PSSGSFMFKL
210 220 230 240 250
ETLGLPEFFG FTSFLEVYRS GPWDGLRFSG ILEMQQWDDI IYNFTENREE
260 270 280 290 300
VAYTFRVTDH NSYSRLTINT VGRLEGFMWE PTQQEWNMFW FMPKDTCDLY
310 320 330 340 350
GICGPYAYCD MSTSPTCNCI KGFQPLSPQD WASGDVTGRC RRKTQLTCGE
360 370 380 390 400
DRFFRLMNMK IPATTAAIVD KRIGLKECEE KCKTHCNCTA YANSDIRNGG
410 420 430 440 450
SGCIIWIGEF RDIRNYAADG QDLFVRLAAA EFGERRTIRG KIIGLIIGIS
460 470 480 490 500
LMLVLSFIIY CFWKKKQKRA RATAAPIGYR DRIQELIITN GVVMSSGRRL
510 520 530 540 550
LGEEEDLELP LTEFETVVMA TENFSDSNIL GRGGFGIVYK GRLLDGQEIA
560 570 580 590 600
VKRLSEMSSQ GTNEFKNEVR LIARLQHINL VRLLSCCIYA DEKILIYEYL
610 620 630 640 650
ENGSLDSHLF ETTQSSNKLN WQTRFSIING IARGLLYLHQ DSRFKIIHRD
660 670 680 690 700
LKASNVLLDK NMTPKISDFG MARIFERDET EANTRKVVGT YGYMSPEYAM
710 720 730 740 750
EGIFSVKSDV FSFGVLVLEI VSGKRNRGFH NSGQDNNLLG YTWENWKEGK
760 770 780 790 800
GLEIVDSIIV DSSSSMSLFQ PHEVLRCIQI GLLCVQERAE DRPKMSSVVL
810 820 830 840 850
MLGSEKGEIP QPKRPGYCVG RSSLDTADSS SSTKRDSESL TVNQITVSVI

NAR
Length:853
Mass (Da):96,764
Last modified:October 19, 2011 - v1
Checksum:i9A74E475EEA40204
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti45S → N in strain: cv. Ge-1. 1 Publication1
Natural varianti269N → Y in strain: cv. Ge-1. 1 Publication1
Natural varianti278M → T in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication1
Natural varianti373I → T in strain: cv. Nok-0. 1 Publication1
Natural varianti415N → K in strain: cv. Ca-0 and cv. Co. 1 Publication1
Natural varianti420G → A in strain: cv. Fi-1. 1 Publication1
Natural varianti438I → S in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication1
Natural varianti562T → I in strain: cv. Ca-0. 1 Publication1
Natural varianti612T → R in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication1
Natural varianti630G → D in strain: cv. Ca-0. 1 Publication1
Natural varianti676E → Q in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication1
Natural varianti829 – 837Missing in strain: cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF692484 Genomic DNA. Translation: ABU54597.1.
EF692485 Genomic DNA. Translation: ABU54598.1.
EF692488 mRNA. Translation: ABU54595.1.
EF692489 mRNA. Translation: ABU54596.1.
GU723782 mRNA. Translation: ADG01645.1.
GU723783 mRNA. Translation: ADG01646.1.
GU723784 mRNA. Translation: ADG01647.1.
GU723785 mRNA. Translation: ADG01648.1.
GU723786 mRNA. Translation: ADG01649.1.
GU723787 mRNA. Translation: ADG01650.1.
GU723788 mRNA. Translation: ADG01651.1.
GU723789 mRNA. Translation: ADG01652.1.
GU723790 mRNA. Translation: ADG01653.1.
GU723791 mRNA. Translation: ADG01654.1.
GU723866 Genomic DNA. Translation: ADG01729.1.
GU723867 Genomic DNA. Translation: ADG01730.1.
GU723870 Genomic DNA. Translation: ADG01731.1.
GU723871 Genomic DNA. Translation: ADG01732.1.
GU723872 Genomic DNA. Translation: ADG01733.1.
GU723873 Genomic DNA. Translation: ADG01734.1.
GU723874 Genomic DNA. Translation: ADG01735.1.
GU723875 Genomic DNA. Translation: ADG01736.1.
GU723876 Genomic DNA. Translation: ADG01737.1.
GU723877 Genomic DNA. Translation: ADG01738.1.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC
Protein Spotlight

Do it yourself - Issue 128 of May 2011

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF692484 Genomic DNA. Translation: ABU54597.1.
EF692485 Genomic DNA. Translation: ABU54598.1.
EF692488 mRNA. Translation: ABU54595.1.
EF692489 mRNA. Translation: ABU54596.1.
GU723782 mRNA. Translation: ADG01645.1.
GU723783 mRNA. Translation: ADG01646.1.
GU723784 mRNA. Translation: ADG01647.1.
GU723785 mRNA. Translation: ADG01648.1.
GU723786 mRNA. Translation: ADG01649.1.
GU723787 mRNA. Translation: ADG01650.1.
GU723788 mRNA. Translation: ADG01651.1.
GU723789 mRNA. Translation: ADG01652.1.
GU723790 mRNA. Translation: ADG01653.1.
GU723791 mRNA. Translation: ADG01654.1.
GU723866 Genomic DNA. Translation: ADG01729.1.
GU723867 Genomic DNA. Translation: ADG01730.1.
GU723870 Genomic DNA. Translation: ADG01731.1.
GU723871 Genomic DNA. Translation: ADG01732.1.
GU723872 Genomic DNA. Translation: ADG01733.1.
GU723873 Genomic DNA. Translation: ADG01734.1.
GU723874 Genomic DNA. Translation: ADG01735.1.
GU723875 Genomic DNA. Translation: ADG01736.1.
GU723876 Genomic DNA. Translation: ADG01737.1.
GU723877 Genomic DNA. Translation: ADG01738.1.

3D structure databases

ProteinModelPortaliP0DH86.
SMRiP0DH86.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
2.90.10.10. 1 hit.
InterProiIPR001480. Bulb-type_lectin_dom.
IPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR003609. Pan_app.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR022126. S-locus_recpt_kinase.
IPR021820. S-locus_recpt_kinase_C.
IPR000858. S_locus_glycoprot_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR024171. SRK-like_kinase.
[Graphical view]
PfamiPF01453. B_lectin. 1 hit.
PF11883. DUF3403. 1 hit.
PF12398. DUF3660. 1 hit.
PF08276. PAN_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00954. S_locus_glycop. 1 hit.
[Graphical view]
PIRSFiPIRSF000641. SRK. 1 hit.
SMARTiSM00108. B_lectin. 1 hit.
SM00473. PAN_AP. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF51110. SSF51110. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50927. BULB_LECTIN. 1 hit.
PS50948. PAN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRK_ARATH
AccessioniPrimary (citable) accession number: P0DH86
Secondary accession number(s): B0F2A9
, B0F2B0, D6NTN9, D6NTP0, D6NTP1, D6NTP2, D6NTP6, D6NTP7, D6NTP8, O81904
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 19, 2011
Last modified: November 2, 2016
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Has been shown to be a pseudogene in cv. Columbia (AC P0DH87) due to a frameshift mutation that introduces a premature stop codon in this strain. The sequence shown is from strains cv. Pog-0 and cv. Wei-1.Curated

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.