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P0DH86

- SRK_ARATH

UniProt

P0DH86 - SRK_ARATH

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Protein
G-type lectin S-receptor-like serine/threonine-protein kinase SRK
Gene
SRK
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Female specificity determinant of self-incompatibility.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521ATP By similarity
Active sitei650 – 6501Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi530 – 5389ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calmodulin binding Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. defense response to bacterium, incompatible interaction Source: UniProtKB
  2. protein autophosphorylation Source: UniProtKB
  3. recognition of pollen Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Lectin, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
G-type lectin S-receptor-like serine/threonine-protein kinase SRK (EC:2.7.11.1)
Gene namesi
Name:SRK
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 441410Extracellular Reviewed prediction
Add
BLAST
Transmembranei442 – 46221Helical; Reviewed prediction
Add
BLAST
Topological domaini463 – 853391Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131 Reviewed prediction
Add
BLAST
Chaini32 – 853822G-type lectin S-receptor-like serine/threonine-protein kinase SRK
PRO_0000401305Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi46 – 461N-linked (GlcNAc...) Reviewed prediction
Glycosylationi120 – 1201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi147 – 1471N-linked (GlcNAc...) Reviewed prediction
Glycosylationi243 – 2431N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi297 ↔ 309 By similarity
Disulfide bondi303 ↔ 317 By similarity
Disulfide bondi378 ↔ 403 By similarity
Disulfide bondi382 ↔ 388 By similarity
Glycosylationi387 – 3871N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP0DH86.

Structurei

3D structure databases

ProteinModelPortaliP0DH86.
SMRiP0DH86. Positions 33-161, 508-804.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 154121Bulb-type lectin
Add
BLAST
Domaini293 – 32937EGF-like; atypical
Add
BLAST
Domaini348 – 42881PAN
Add
BLAST
Domaini524 – 802279Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni613 – 63119CaM-binding By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi829 – 8324Poly-Ser

Sequence similaritiesi

Contains 1 EGF-like domain.
Contains 1 PAN domain.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
2.90.10.10. 1 hit.
InterProiIPR001480. Bulb-type_lectin_dom.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR011009. Kinase-like_dom.
IPR013227. PAN-2_domain.
IPR003609. Pan_app.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR022126. S-locus_recpt_kinase.
IPR021820. S-locus_recpt_kinase_C.
IPR000858. S_locus_glycoprot.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR024171. SRK_like_kinase.
[Graphical view]
PfamiPF01453. B_lectin. 1 hit.
PF11883. DUF3403. 1 hit.
PF12398. DUF3660. 1 hit.
PF08276. PAN_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00954. S_locus_glycop. 1 hit.
[Graphical view]
PIRSFiPIRSF000641. SRK. 1 hit.
SMARTiSM00108. B_lectin. 1 hit.
SM00181. EGF. 1 hit.
SM00473. PAN_AP. 1 hit.
[Graphical view]
SUPFAMiSSF51110. SSF51110. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50927. BULB_LECTIN. 1 hit.
PS50948. PAN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0DH86-1 [UniParc]FASTAAdd to Basket

« Hide

MRGELPNKHH SYTFFVFLFF FLILFPDLSI SVNTLSATES LTISSNKTIV    50
SPGGVFELGF FRILGDSWYL GIWYKKISQR TYVWVANRDT PLSNPIGILK 100
ISNANLVILD NSDTHVWSTN LTGAVRSSVV AELLDNGNFV LRGSKINESD 150
EFLWQSFDFP TDTLLPQMKL GRDHKRGLNR FVTSWKSSFD PSSGSFMFKL 200
ETLGLPEFFG FTSFLEVYRS GPWDGLRFSG ILEMQQWDDI IYNFTENREE 250
VAYTFRVTDH NSYSRLTINT VGRLEGFMWE PTQQEWNMFW FMPKDTCDLY 300
GICGPYAYCD MSTSPTCNCI KGFQPLSPQD WASGDVTGRC RRKTQLTCGE 350
DRFFRLMNMK IPATTAAIVD KRIGLKECEE KCKTHCNCTA YANSDIRNGG 400
SGCIIWIGEF RDIRNYAADG QDLFVRLAAA EFGERRTIRG KIIGLIIGIS 450
LMLVLSFIIY CFWKKKQKRA RATAAPIGYR DRIQELIITN GVVMSSGRRL 500
LGEEEDLELP LTEFETVVMA TENFSDSNIL GRGGFGIVYK GRLLDGQEIA 550
VKRLSEMSSQ GTNEFKNEVR LIARLQHINL VRLLSCCIYA DEKILIYEYL 600
ENGSLDSHLF ETTQSSNKLN WQTRFSIING IARGLLYLHQ DSRFKIIHRD 650
LKASNVLLDK NMTPKISDFG MARIFERDET EANTRKVVGT YGYMSPEYAM 700
EGIFSVKSDV FSFGVLVLEI VSGKRNRGFH NSGQDNNLLG YTWENWKEGK 750
GLEIVDSIIV DSSSSMSLFQ PHEVLRCIQI GLLCVQERAE DRPKMSSVVL 800
MLGSEKGEIP QPKRPGYCVG RSSLDTADSS SSTKRDSESL TVNQITVSVI 850
NAR 853
Length:853
Mass (Da):96,764
Last modified:October 19, 2011 - v1
Checksum:i9A74E475EEA40204
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti45 – 451S → N in strain: cv. Ge-1. 1 Publication
Natural varianti269 – 2691N → Y in strain: cv. Ge-1. 1 Publication
Natural varianti278 – 2781M → T in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
Natural varianti373 – 3731I → T in strain: cv. Nok-0. 1 Publication
Natural varianti415 – 4151N → K in strain: cv. Ca-0 and cv. Co. 1 Publication
Natural varianti420 – 4201G → A in strain: cv. Fi-1. 1 Publication
Natural varianti438 – 4381I → S in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
Natural varianti562 – 5621T → I in strain: cv. Ca-0. 1 Publication
Natural varianti612 – 6121T → R in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
Natural varianti630 – 6301G → D in strain: cv. Ca-0. 1 Publication
Natural varianti676 – 6761E → Q in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
Natural varianti829 – 8379Missing in strain: cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF692484 Genomic DNA. Translation: ABU54597.1.
EF692485 Genomic DNA. Translation: ABU54598.1.
EF692488 mRNA. Translation: ABU54595.1.
EF692489 mRNA. Translation: ABU54596.1.
GU723782 mRNA. Translation: ADG01645.1.
GU723783 mRNA. Translation: ADG01646.1.
GU723784 mRNA. Translation: ADG01647.1.
GU723785 mRNA. Translation: ADG01648.1.
GU723786 mRNA. Translation: ADG01649.1.
GU723787 mRNA. Translation: ADG01650.1.
GU723788 mRNA. Translation: ADG01651.1.
GU723789 mRNA. Translation: ADG01652.1.
GU723790 mRNA. Translation: ADG01653.1.
GU723791 mRNA. Translation: ADG01654.1.
GU723866 Genomic DNA. Translation: ADG01729.1.
GU723867 Genomic DNA. Translation: ADG01730.1.
GU723870 Genomic DNA. Translation: ADG01731.1.
GU723871 Genomic DNA. Translation: ADG01732.1.
GU723872 Genomic DNA. Translation: ADG01733.1.
GU723873 Genomic DNA. Translation: ADG01734.1.
GU723874 Genomic DNA. Translation: ADG01735.1.
GU723875 Genomic DNA. Translation: ADG01736.1.
GU723876 Genomic DNA. Translation: ADG01737.1.
GU723877 Genomic DNA. Translation: ADG01738.1.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC
Protein Spotlight

Do it yourself - Issue 128 of May 2011

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF692484 Genomic DNA. Translation: ABU54597.1 .
EF692485 Genomic DNA. Translation: ABU54598.1 .
EF692488 mRNA. Translation: ABU54595.1 .
EF692489 mRNA. Translation: ABU54596.1 .
GU723782 mRNA. Translation: ADG01645.1 .
GU723783 mRNA. Translation: ADG01646.1 .
GU723784 mRNA. Translation: ADG01647.1 .
GU723785 mRNA. Translation: ADG01648.1 .
GU723786 mRNA. Translation: ADG01649.1 .
GU723787 mRNA. Translation: ADG01650.1 .
GU723788 mRNA. Translation: ADG01651.1 .
GU723789 mRNA. Translation: ADG01652.1 .
GU723790 mRNA. Translation: ADG01653.1 .
GU723791 mRNA. Translation: ADG01654.1 .
GU723866 Genomic DNA. Translation: ADG01729.1 .
GU723867 Genomic DNA. Translation: ADG01730.1 .
GU723870 Genomic DNA. Translation: ADG01731.1 .
GU723871 Genomic DNA. Translation: ADG01732.1 .
GU723872 Genomic DNA. Translation: ADG01733.1 .
GU723873 Genomic DNA. Translation: ADG01734.1 .
GU723874 Genomic DNA. Translation: ADG01735.1 .
GU723875 Genomic DNA. Translation: ADG01736.1 .
GU723876 Genomic DNA. Translation: ADG01737.1 .
GU723877 Genomic DNA. Translation: ADG01738.1 .

3D structure databases

ProteinModelPortali P0DH86.
SMRi P0DH86. Positions 33-161, 508-804.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P0DH86.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
2.90.10.10. 1 hit.
InterProi IPR001480. Bulb-type_lectin_dom.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR011009. Kinase-like_dom.
IPR013227. PAN-2_domain.
IPR003609. Pan_app.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR022126. S-locus_recpt_kinase.
IPR021820. S-locus_recpt_kinase_C.
IPR000858. S_locus_glycoprot.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR024171. SRK_like_kinase.
[Graphical view ]
Pfami PF01453. B_lectin. 1 hit.
PF11883. DUF3403. 1 hit.
PF12398. DUF3660. 1 hit.
PF08276. PAN_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00954. S_locus_glycop. 1 hit.
[Graphical view ]
PIRSFi PIRSF000641. SRK. 1 hit.
SMARTi SM00108. B_lectin. 1 hit.
SM00181. EGF. 1 hit.
SM00473. PAN_AP. 1 hit.
[Graphical view ]
SUPFAMi SSF51110. SSF51110. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEi PS50927. BULB_LECTIN. 1 hit.
PS50948. PAN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Independent origins of self-compatibility in Arabidopsis thaliana."
    Shimizu K.K., Shimizu-Inatsugi R., Tsuchimatsu T., Purugganan M.D.
    Mol. Ecol. 17:704-714(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT THR-373.
    Strain: cv. Nok-0 and cv. Pog-0.
  2. "Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene."
    Tsuchimatsu T., Suwabe K., Shimizu-Inatsugi R., Isokawa S., Pavlidis P., Stadler T., Suzuki G., Takayama S., Watanabe M., Shimizu K.K.
    Nature 464:1342-1346(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS ASN-45; TYR-269; THR-278; LYS-415; ALA-420; SER-438; ILE-562; ARG-612; ASP-630; GLN-676 AND 829-SER--SER-837 DEL, FUNCTION.
    Strain: cv. Ca-0, cv. Co, cv. Di-1, cv. Fi-1, cv. Ge-1, cv. Gie, cv. Old-1, cv. Uk-3, cv. Wassilewskija and cv. Wei-1.
  3. "Natural variation in expression of self-incompatibility in Arabidopsis thaliana: implications for the evolution of selfing."
    Nasrallah M.E., Liu P., Sherman-Broyles S., Boggs N.A., Nasrallah J.B.
    Proc. Natl. Acad. Sci. U.S.A. 101:16070-16074(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "S locus genes and the evolution of self-fertility in Arabidopsis thaliana."
    Sherman-Broyles S., Boggs N., Farkas A., Liu P., Vrebalov J., Nasrallah M.E., Nasrallah J.B.
    Plant Cell 19:94-106(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REVIEW.
  5. Cited for: FUNCTION.

Entry informationi

Entry nameiSRK_ARATH
AccessioniPrimary (citable) accession number: P0DH86
Secondary accession number(s): B0F2A9
, B0F2B0, D6NTN9, D6NTP0, D6NTP1, D6NTP2, D6NTP6, D6NTP7, D6NTP8, O81904
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 19, 2011
Last modified: March 19, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Has been shown to be a pseudogene in cv. Columbia (AC P0DH87) due to a frameshift mutation that introduces a premature stop codon in this strain. The sequence shown is from strains cv. Pog-0 and cv. Wei-1.

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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