P0DH86 (SRK_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G-type lectin S-receptor-like serine/threonine-protein kinase SRK
EC=2.7.11.1

Gene names

Name:

SRK

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	853 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Female specificity determinant of self-incompatibility. Ref.2 Ref.3 Ref.4 Ref.5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cell membrane; Single-pass type I membrane protein By similarity.
Miscellaneous	Has been shown to be a pseudogene in cv. Columbia due to a frameshift mutation that introduces a premature stop codon in this strain. The sequence shown is from strains cv. Pog-0 and cv. Wei-1.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 bulb-type lectin domain. Contains 1 EGF-like domain. Contains 1 PAN domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Lectin Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological_process	defense response to bacterium, incompatible interaction Inferred from sequence or structural similarity. Source: UniProtKB protein autophosphorylation Inferred from sequence or structural similarity. Source: UniProtKB recognition of pollen Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane part Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 853	822	G-type lectin S-receptor-like serine/threonine-protein kinase SRK		PRO_0000401305
Regions
	Topological domain	32 – 441	410	Extracellular Potential
	Transmembrane	442 – 462	21	Helical; Potential
	Topological domain	463 – 853	391	Cytoplasmic Potential
	Domain	34 – 154	121	Bulb-type lectin
	Domain	293 – 329	37	EGF-like; atypical
	Domain	348 – 428	81	PAN
	Domain	524 – 802	279	Protein kinase
	Nucleotide binding	530 – 538	9	ATP By similarity
	Region	613 – 631	19	CaM-binding By similarity
	Compositional bias	829 – 832	4	Poly-Ser
Sites
	Active site	650	1	Proton acceptor By similarity
	Binding site	552	1	ATP By similarity
Amino acid modifications
	Modified residue	615	1	Phosphoserine By similarity
	Modified residue	654	1	Phosphoserine By similarity
	Modified residue	667	1	Phosphoserine By similarity
	Modified residue	684	1	Phosphothreonine By similarity
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	120	1	N-linked (GlcNAc...) Potential
	Glycosylation	147	1	N-linked (GlcNAc...) Potential
	Glycosylation	243	1	N-linked (GlcNAc...) Potential
	Glycosylation	387	1	N-linked (GlcNAc...) Potential
	Disulfide bond	297 ↔ 309		By similarity
	Disulfide bond	303 ↔ 317		By similarity
	Disulfide bond	378 ↔ 403		By similarity
	Disulfide bond	382 ↔ 388		By similarity
Natural variations
	Natural variant	45	1	S → N in strain: cv. Ge-1. Ref.2
	Natural variant	269	1	N → Y in strain: cv. Ge-1. Ref.2
	Natural variant	278	1	M → T in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. Ref.2
	Natural variant	373	1	I → T in strain: cv. Nok-0. Ref.1
	Natural variant	415	1	N → K in strain: cv. Ca-0 and cv. Co. Ref.2
	Natural variant	420	1	G → A in strain: cv. Fi-1. Ref.2
	Natural variant	438	1	I → S in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. Ref.2
	Natural variant	562	1	T → I in strain: cv. Ca-0. Ref.2
	Natural variant	612	1	T → R in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. Ref.2
	Natural variant	630	1	G → D in strain: cv. Ca-0. Ref.2
	Natural variant	676	1	E → Q in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. Ref.2
	Natural variant	829 – 837	9	Missing in strain: cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija.

Sequences

	Sequence		Length	Mass (Da)
	P0DH86 [UniParc]. Last modified October 19, 2011. Version 1. Checksum: 9A74E475EEA40204	FASTA	853	96,764
	10 20 30 40 50 60 MRGELPNKHH SYTFFVFLFF FLILFPDLSI SVNTLSATES LTISSNKTIV SPGGVFELGF 70 80 90 100 110 120 FRILGDSWYL GIWYKKISQR TYVWVANRDT PLSNPIGILK ISNANLVILD NSDTHVWSTN 130 140 150 160 170 180 LTGAVRSSVV AELLDNGNFV LRGSKINESD EFLWQSFDFP TDTLLPQMKL GRDHKRGLNR 190 200 210 220 230 240 FVTSWKSSFD PSSGSFMFKL ETLGLPEFFG FTSFLEVYRS GPWDGLRFSG ILEMQQWDDI 250 260 270 280 290 300 IYNFTENREE VAYTFRVTDH NSYSRLTINT VGRLEGFMWE PTQQEWNMFW FMPKDTCDLY 310 320 330 340 350 360 GICGPYAYCD MSTSPTCNCI KGFQPLSPQD WASGDVTGRC RRKTQLTCGE DRFFRLMNMK 370 380 390 400 410 420 IPATTAAIVD KRIGLKECEE KCKTHCNCTA YANSDIRNGG SGCIIWIGEF RDIRNYAADG 430 440 450 460 470 480 QDLFVRLAAA EFGERRTIRG KIIGLIIGIS LMLVLSFIIY CFWKKKQKRA RATAAPIGYR 490 500 510 520 530 540 DRIQELIITN GVVMSSGRRL LGEEEDLELP LTEFETVVMA TENFSDSNIL GRGGFGIVYK 550 560 570 580 590 600 GRLLDGQEIA VKRLSEMSSQ GTNEFKNEVR LIARLQHINL VRLLSCCIYA DEKILIYEYL 610 620 630 640 650 660 ENGSLDSHLF ETTQSSNKLN WQTRFSIING IARGLLYLHQ DSRFKIIHRD LKASNVLLDK 670 680 690 700 710 720 NMTPKISDFG MARIFERDET EANTRKVVGT YGYMSPEYAM EGIFSVKSDV FSFGVLVLEI 730 740 750 760 770 780 VSGKRNRGFH NSGQDNNLLG YTWENWKEGK GLEIVDSIIV DSSSSMSLFQ PHEVLRCIQI 790 800 810 820 830 840 GLLCVQERAE DRPKMSSVVL MLGSEKGEIP QPKRPGYCVG RSSLDTADSS SSTKRDSESL 850 TVNQITVSVI NAR

References

[1]	"Independent origins of self-compatibility in Arabidopsis thaliana." Shimizu K.K., Shimizu-Inatsugi R., Tsuchimatsu T., Purugganan M.D. Mol. Ecol. 17:704-714(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT THR-373. Strain: cv. Nok-0 and cv. Pog-0.
[2]	"Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene." Tsuchimatsu T., Suwabe K., Shimizu-Inatsugi R., Isokawa S., Pavlidis P., Stadler T., Suzuki G., Takayama S., Watanabe M., Shimizu K.K. Nature 464:1342-1346(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS ASN-45; TYR-269; THR-278; LYS-415; ALA-420; SER-438; ILE-562; ARG-612; ASP-630; GLN-676 AND 829-SER--SER-837 DEL, FUNCTION. Strain: cv. Ca-0, cv. Co, cv. Di-1, cv. Fi-1, cv. Ge-1, cv. Gie, cv. Old-1, cv. Uk-3, cv. Wassilewskija and cv. Wei-1.
[3]	"Natural variation in expression of self-incompatibility in Arabidopsis thaliana: implications for the evolution of selfing." Nasrallah M.E., Liu P., Sherman-Broyles S., Boggs N.A., Nasrallah J.B. Proc. Natl. Acad. Sci. U.S.A. 101:16070-16074(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"S locus genes and the evolution of self-fertility in Arabidopsis thaliana." Sherman-Broyles S., Boggs N., Farkas A., Liu P., Vrebalov J., Nasrallah M.E., Nasrallah J.B. Plant Cell 19:94-106(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, REVIEW.
[5]	"The evolution of selfing in Arabidopsis thaliana." Tang C., Toomajian C., Sherman-Broyles S., Plagnol V., Guo Y.-L., Hu T.T., Clark R.M., Nasrallah J.B., Weigel D., Nordborg M. Science 317:1070-1072(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Web resources

PlantP kinase Classification PPC

Protein Spotlight

Do it yourself - Issue 128 of May 2011

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF692484 Genomic DNA. Translation: ABU54597.1. EF692485 Genomic DNA. Translation: ABU54598.1. EF692488 mRNA. Translation: ABU54595.1. EF692489 mRNA. Translation: ABU54596.1. GU723782 mRNA. Translation: ADG01645.1. GU723783 mRNA. Translation: ADG01646.1. GU723784 mRNA. Translation: ADG01647.1. GU723785 mRNA. Translation: ADG01648.1. GU723786 mRNA. Translation: ADG01649.1. GU723787 mRNA. Translation: ADG01650.1. GU723788 mRNA. Translation: ADG01651.1. GU723789 mRNA. Translation: ADG01652.1. GU723790 mRNA. Translation: ADG01653.1. GU723791 mRNA. Translation: ADG01654.1. GU723866 Genomic DNA. Translation: ADG01729.1. GU723867 Genomic DNA. Translation: ADG01730.1. GU723870 Genomic DNA. Translation: ADG01731.1. GU723871 Genomic DNA. Translation: ADG01732.1. GU723872 Genomic DNA. Translation: ADG01733.1. GU723873 Genomic DNA. Translation: ADG01734.1. GU723874 Genomic DNA. Translation: ADG01735.1. GU723875 Genomic DNA. Translation: ADG01736.1. GU723876 Genomic DNA. Translation: ADG01737.1. GU723877 Genomic DNA. Translation: ADG01738.1.
3D structure databases
HSSP	HSSP built from PDB template 3LCK based on UniProtKB P06239.
ProteinModelPortal	P0DH86.
SMR	P0DH86. Positions 33-161, 508-804.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.120.200. 1 hit. 2.90.10.10. 1 hit.
InterPro	IPR001480. Bulb-type_lectin_dom. IPR013320. ConA-like_subgrp. IPR000742. EG-like_dom. IPR011009. Kinase-like_dom. IPR013227. PAN-2_domain. IPR003609. Pan_app. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR022126. S-locus_recpt_kinase. IPR021820. S-locus_recpt_kinase_C. IPR000858. S_locus_glycoprot. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. IPR024171. SRK_like_kinase. [Graphical view]
Pfam	PF01453. B_lectin. 1 hit. PF11883. DUF3403. 1 hit. PF12398. DUF3660. 1 hit. PF08276. PAN_2. 1 hit. PF07714. Pkinase_Tyr. 1 hit. PF00954. S_locus_glycop. 1 hit. [Graphical view]
PIRSF	PIRSF000641. SRK. 1 hit.
SMART	SM00108. B_lectin. 1 hit. SM00181. EGF. 1 hit. SM00473. PAN_AP. 1 hit. [Graphical view]
SUPFAM	SSF51110. B_lectin. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS50927. BULB_LECTIN. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. False negative. PS50026. EGF_3. False negative. PS50948. PAN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRK_ARATH

Accession

Primary (citable) accession number: P0DH86
Secondary accession number(s): B0F2A9 , B0F2B0, D6NTN9, D6NTP0, D6NTP1, D6NTP2, D6NTP6, D6NTP7, D6NTP8, O81904

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	October 19, 2011
Last modified:	March 6, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program