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P0DH86 (SRK_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-type lectin S-receptor-like serine/threonine-protein kinase SRK

EC=2.7.11.1
Gene names
Name:SRK
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length853 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Female specificity determinant of self-incompatibility. Ref.2 Ref.3 Ref.4 Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 bulb-type lectin domain.

Contains 1 EGF-like domain.

Contains 1 PAN domain.

Contains 1 protein kinase domain.

Caution

Has been shown to be a pseudogene in cv. Columbia (AC P0DH87) due to a frameshift mutation that introduces a premature stop codon in this strain. The sequence shown is from strains cv. Pog-0 and cv. Wei-1.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 853822G-type lectin S-receptor-like serine/threonine-protein kinase SRK
PRO_0000401305

Regions

Topological domain32 – 441410Extracellular Potential
Transmembrane442 – 46221Helical; Potential
Topological domain463 – 853391Cytoplasmic Potential
Domain34 – 154121Bulb-type lectin
Domain293 – 32937EGF-like; atypical
Domain348 – 42881PAN
Domain524 – 802279Protein kinase
Nucleotide binding530 – 5389ATP By similarity
Region613 – 63119CaM-binding By similarity
Compositional bias829 – 8324Poly-Ser

Sites

Active site6501Proton acceptor By similarity
Binding site5521ATP By similarity

Amino acid modifications

Glycosylation461N-linked (GlcNAc...) Potential
Glycosylation1201N-linked (GlcNAc...) Potential
Glycosylation1471N-linked (GlcNAc...) Potential
Glycosylation2431N-linked (GlcNAc...) Potential
Glycosylation3871N-linked (GlcNAc...) Potential
Disulfide bond297 ↔ 309 By similarity
Disulfide bond303 ↔ 317 By similarity
Disulfide bond378 ↔ 403 By similarity
Disulfide bond382 ↔ 388 By similarity

Natural variations

Natural variant451S → N in strain: cv. Ge-1. Ref.2
Natural variant2691N → Y in strain: cv. Ge-1. Ref.2
Natural variant2781M → T in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. Ref.2
Natural variant3731I → T in strain: cv. Nok-0. Ref.1
Natural variant4151N → K in strain: cv. Ca-0 and cv. Co. Ref.2
Natural variant4201G → A in strain: cv. Fi-1. Ref.2
Natural variant4381I → S in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. Ref.2
Natural variant5621T → I in strain: cv. Ca-0. Ref.2
Natural variant6121T → R in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. Ref.2
Natural variant6301G → D in strain: cv. Ca-0. Ref.2
Natural variant6761E → Q in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. Ref.2
Natural variant829 – 8379Missing in strain: cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija.

Sequences

Sequence LengthMass (Da)Tools
P0DH86 [UniParc].

Last modified October 19, 2011. Version 1.
Checksum: 9A74E475EEA40204

FASTA85396,764
        10         20         30         40         50         60 
MRGELPNKHH SYTFFVFLFF FLILFPDLSI SVNTLSATES LTISSNKTIV SPGGVFELGF 

        70         80         90        100        110        120 
FRILGDSWYL GIWYKKISQR TYVWVANRDT PLSNPIGILK ISNANLVILD NSDTHVWSTN 

       130        140        150        160        170        180 
LTGAVRSSVV AELLDNGNFV LRGSKINESD EFLWQSFDFP TDTLLPQMKL GRDHKRGLNR 

       190        200        210        220        230        240 
FVTSWKSSFD PSSGSFMFKL ETLGLPEFFG FTSFLEVYRS GPWDGLRFSG ILEMQQWDDI 

       250        260        270        280        290        300 
IYNFTENREE VAYTFRVTDH NSYSRLTINT VGRLEGFMWE PTQQEWNMFW FMPKDTCDLY 

       310        320        330        340        350        360 
GICGPYAYCD MSTSPTCNCI KGFQPLSPQD WASGDVTGRC RRKTQLTCGE DRFFRLMNMK 

       370        380        390        400        410        420 
IPATTAAIVD KRIGLKECEE KCKTHCNCTA YANSDIRNGG SGCIIWIGEF RDIRNYAADG 

       430        440        450        460        470        480 
QDLFVRLAAA EFGERRTIRG KIIGLIIGIS LMLVLSFIIY CFWKKKQKRA RATAAPIGYR 

       490        500        510        520        530        540 
DRIQELIITN GVVMSSGRRL LGEEEDLELP LTEFETVVMA TENFSDSNIL GRGGFGIVYK 

       550        560        570        580        590        600 
GRLLDGQEIA VKRLSEMSSQ GTNEFKNEVR LIARLQHINL VRLLSCCIYA DEKILIYEYL 

       610        620        630        640        650        660 
ENGSLDSHLF ETTQSSNKLN WQTRFSIING IARGLLYLHQ DSRFKIIHRD LKASNVLLDK 

       670        680        690        700        710        720 
NMTPKISDFG MARIFERDET EANTRKVVGT YGYMSPEYAM EGIFSVKSDV FSFGVLVLEI 

       730        740        750        760        770        780 
VSGKRNRGFH NSGQDNNLLG YTWENWKEGK GLEIVDSIIV DSSSSMSLFQ PHEVLRCIQI 

       790        800        810        820        830        840 
GLLCVQERAE DRPKMSSVVL MLGSEKGEIP QPKRPGYCVG RSSLDTADSS SSTKRDSESL 

       850 
TVNQITVSVI NAR 

« Hide

References

[1]"Independent origins of self-compatibility in Arabidopsis thaliana."
Shimizu K.K., Shimizu-Inatsugi R., Tsuchimatsu T., Purugganan M.D.
Mol. Ecol. 17:704-714(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT THR-373.
Strain: cv. Nok-0 and cv. Pog-0.
[2]"Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene."
Tsuchimatsu T., Suwabe K., Shimizu-Inatsugi R., Isokawa S., Pavlidis P., Stadler T., Suzuki G., Takayama S., Watanabe M., Shimizu K.K.
Nature 464:1342-1346(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS ASN-45; TYR-269; THR-278; LYS-415; ALA-420; SER-438; ILE-562; ARG-612; ASP-630; GLN-676 AND 829-SER--SER-837 DEL, FUNCTION.
Strain: cv. Ca-0, cv. Co, cv. Di-1, cv. Fi-1, cv. Ge-1, cv. Gie, cv. Old-1, cv. Uk-3, cv. Wassilewskija and cv. Wei-1.
[3]"Natural variation in expression of self-incompatibility in Arabidopsis thaliana: implications for the evolution of selfing."
Nasrallah M.E., Liu P., Sherman-Broyles S., Boggs N.A., Nasrallah J.B.
Proc. Natl. Acad. Sci. U.S.A. 101:16070-16074(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"S locus genes and the evolution of self-fertility in Arabidopsis thaliana."
Sherman-Broyles S., Boggs N., Farkas A., Liu P., Vrebalov J., Nasrallah M.E., Nasrallah J.B.
Plant Cell 19:94-106(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, REVIEW.
[5]"The evolution of selfing in Arabidopsis thaliana."
Tang C., Toomajian C., Sherman-Broyles S., Plagnol V., Guo Y.-L., Hu T.T., Clark R.M., Nasrallah J.B., Weigel D., Nordborg M.
Science 317:1070-1072(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Web resources

PlantP kinase Classification PPC
Protein Spotlight

Do it yourself - Issue 128 of May 2011

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF692484 Genomic DNA. Translation: ABU54597.1.
EF692485 Genomic DNA. Translation: ABU54598.1.
EF692488 mRNA. Translation: ABU54595.1.
EF692489 mRNA. Translation: ABU54596.1.
GU723782 mRNA. Translation: ADG01645.1.
GU723783 mRNA. Translation: ADG01646.1.
GU723784 mRNA. Translation: ADG01647.1.
GU723785 mRNA. Translation: ADG01648.1.
GU723786 mRNA. Translation: ADG01649.1.
GU723787 mRNA. Translation: ADG01650.1.
GU723788 mRNA. Translation: ADG01651.1.
GU723789 mRNA. Translation: ADG01652.1.
GU723790 mRNA. Translation: ADG01653.1.
GU723791 mRNA. Translation: ADG01654.1.
GU723866 Genomic DNA. Translation: ADG01729.1.
GU723867 Genomic DNA. Translation: ADG01730.1.
GU723870 Genomic DNA. Translation: ADG01731.1.
GU723871 Genomic DNA. Translation: ADG01732.1.
GU723872 Genomic DNA. Translation: ADG01733.1.
GU723873 Genomic DNA. Translation: ADG01734.1.
GU723874 Genomic DNA. Translation: ADG01735.1.
GU723875 Genomic DNA. Translation: ADG01736.1.
GU723876 Genomic DNA. Translation: ADG01737.1.
GU723877 Genomic DNA. Translation: ADG01738.1.

3D structure databases

ProteinModelPortalP0DH86.
SMRP0DH86. Positions 33-161, 508-804.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP0DH86.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.200. 1 hit.
2.90.10.10. 1 hit.
InterProIPR001480. Bulb-type_lectin_dom.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR011009. Kinase-like_dom.
IPR013227. PAN-2_domain.
IPR003609. Pan_app.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR022126. S-locus_recpt_kinase.
IPR021820. S-locus_recpt_kinase_C.
IPR000858. S_locus_glycoprot.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR024171. SRK_like_kinase.
[Graphical view]
PfamPF01453. B_lectin. 1 hit.
PF11883. DUF3403. 1 hit.
PF12398. DUF3660. 1 hit.
PF08276. PAN_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00954. S_locus_glycop. 1 hit.
[Graphical view]
PIRSFPIRSF000641. SRK. 1 hit.
SMARTSM00108. B_lectin. 1 hit.
SM00181. EGF. 1 hit.
SM00473. PAN_AP. 1 hit.
[Graphical view]
SUPFAMSSF51110. SSF51110. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEPS50927. BULB_LECTIN. 1 hit.
PS50948. PAN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSRK_ARATH
AccessionPrimary (citable) accession number: P0DH86
Secondary accession number(s): B0F2A9 expand/collapse secondary AC list , B0F2B0, D6NTN9, D6NTP0, D6NTP1, D6NTP2, D6NTP6, D6NTP7, D6NTP8, O81904
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 19, 2011
Last modified: March 19, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names