Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0DH86

- SRK_ARATH

UniProt

P0DH86 - SRK_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

G-type lectin S-receptor-like serine/threonine-protein kinase SRK

Gene

SRK

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Female specificity determinant of self-incompatibility.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521ATPPROSITE-ProRule annotation
Active sitei650 – 6501Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi530 – 5389ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calmodulin binding Source: UniProtKB
  3. carbohydrate binding Source: UniProtKB-KW
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. defense response to bacterium, incompatible interaction Source: UniProtKB
  2. protein autophosphorylation Source: UniProtKB
  3. recognition of pollen Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Lectin, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
G-type lectin S-receptor-like serine/threonine-protein kinase SRK (EC:2.7.11.1)
Gene namesi
Name:SRK
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 853822G-type lectin S-receptor-like serine/threonine-protein kinase SRKPRO_0000401305Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi243 – 2431N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi297 ↔ 309By similarity
Disulfide bondi303 ↔ 317By similarity
Disulfide bondi378 ↔ 403By similarity
Disulfide bondi382 ↔ 388By similarity
Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP0DH86.

Structurei

3D structure databases

ProteinModelPortaliP0DH86.
SMRiP0DH86. Positions 33-161, 508-804.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 441410ExtracellularSequence AnalysisAdd
BLAST
Topological domaini463 – 853391CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei442 – 46221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 154121Bulb-type lectinPROSITE-ProRule annotationAdd
BLAST
Domaini293 – 32937EGF-like; atypicalAdd
BLAST
Domaini348 – 42881PANPROSITE-ProRule annotationAdd
BLAST
Domaini524 – 802279Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni613 – 63119CaM-bindingBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi829 – 8324Poly-Ser

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 bulb-type lectin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.Curated
Contains 1 PAN domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
2.90.10.10. 1 hit.
InterProiIPR001480. Bulb-type_lectin_dom.
IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR011009. Kinase-like_dom.
IPR013227. PAN-2_domain.
IPR003609. Pan_app.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR022126. S-locus_recpt_kinase.
IPR021820. S-locus_recpt_kinase_C.
IPR000858. S_locus_glycoprot.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR024171. SRK_like_kinase.
[Graphical view]
PfamiPF01453. B_lectin. 1 hit.
PF11883. DUF3403. 1 hit.
PF12398. DUF3660. 1 hit.
PF08276. PAN_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00954. S_locus_glycop. 1 hit.
[Graphical view]
PIRSFiPIRSF000641. SRK. 1 hit.
SMARTiSM00108. B_lectin. 1 hit.
SM00181. EGF. 1 hit.
SM00473. PAN_AP. 1 hit.
[Graphical view]
SUPFAMiSSF51110. SSF51110. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50927. BULB_LECTIN. 1 hit.
PS50948. PAN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0DH86-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRGELPNKHH SYTFFVFLFF FLILFPDLSI SVNTLSATES LTISSNKTIV
60 70 80 90 100
SPGGVFELGF FRILGDSWYL GIWYKKISQR TYVWVANRDT PLSNPIGILK
110 120 130 140 150
ISNANLVILD NSDTHVWSTN LTGAVRSSVV AELLDNGNFV LRGSKINESD
160 170 180 190 200
EFLWQSFDFP TDTLLPQMKL GRDHKRGLNR FVTSWKSSFD PSSGSFMFKL
210 220 230 240 250
ETLGLPEFFG FTSFLEVYRS GPWDGLRFSG ILEMQQWDDI IYNFTENREE
260 270 280 290 300
VAYTFRVTDH NSYSRLTINT VGRLEGFMWE PTQQEWNMFW FMPKDTCDLY
310 320 330 340 350
GICGPYAYCD MSTSPTCNCI KGFQPLSPQD WASGDVTGRC RRKTQLTCGE
360 370 380 390 400
DRFFRLMNMK IPATTAAIVD KRIGLKECEE KCKTHCNCTA YANSDIRNGG
410 420 430 440 450
SGCIIWIGEF RDIRNYAADG QDLFVRLAAA EFGERRTIRG KIIGLIIGIS
460 470 480 490 500
LMLVLSFIIY CFWKKKQKRA RATAAPIGYR DRIQELIITN GVVMSSGRRL
510 520 530 540 550
LGEEEDLELP LTEFETVVMA TENFSDSNIL GRGGFGIVYK GRLLDGQEIA
560 570 580 590 600
VKRLSEMSSQ GTNEFKNEVR LIARLQHINL VRLLSCCIYA DEKILIYEYL
610 620 630 640 650
ENGSLDSHLF ETTQSSNKLN WQTRFSIING IARGLLYLHQ DSRFKIIHRD
660 670 680 690 700
LKASNVLLDK NMTPKISDFG MARIFERDET EANTRKVVGT YGYMSPEYAM
710 720 730 740 750
EGIFSVKSDV FSFGVLVLEI VSGKRNRGFH NSGQDNNLLG YTWENWKEGK
760 770 780 790 800
GLEIVDSIIV DSSSSMSLFQ PHEVLRCIQI GLLCVQERAE DRPKMSSVVL
810 820 830 840 850
MLGSEKGEIP QPKRPGYCVG RSSLDTADSS SSTKRDSESL TVNQITVSVI

NAR
Length:853
Mass (Da):96,764
Last modified:October 19, 2011 - v1
Checksum:i9A74E475EEA40204
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti45 – 451S → N in strain: cv. Ge-1. 1 Publication
Natural varianti269 – 2691N → Y in strain: cv. Ge-1. 1 Publication
Natural varianti278 – 2781M → T in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
Natural varianti373 – 3731I → T in strain: cv. Nok-0. 1 Publication
Natural varianti415 – 4151N → K in strain: cv. Ca-0 and cv. Co. 1 Publication
Natural varianti420 – 4201G → A in strain: cv. Fi-1. 1 Publication
Natural varianti438 – 4381I → S in strain: cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
Natural varianti562 – 5621T → I in strain: cv. Ca-0. 1 Publication
Natural varianti612 – 6121T → R in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
Natural varianti630 – 6301G → D in strain: cv. Ca-0. 1 Publication
Natural varianti676 – 6761E → Q in strain: cv. Di-1, cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication
Natural varianti829 – 8379Missing in strain: cv. Fi-1, cv. Gie, cv. Old-1, cv. Uk-3 and cv. Wassilewskija. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF692484 Genomic DNA. Translation: ABU54597.1.
EF692485 Genomic DNA. Translation: ABU54598.1.
EF692488 mRNA. Translation: ABU54595.1.
EF692489 mRNA. Translation: ABU54596.1.
GU723782 mRNA. Translation: ADG01645.1.
GU723783 mRNA. Translation: ADG01646.1.
GU723784 mRNA. Translation: ADG01647.1.
GU723785 mRNA. Translation: ADG01648.1.
GU723786 mRNA. Translation: ADG01649.1.
GU723787 mRNA. Translation: ADG01650.1.
GU723788 mRNA. Translation: ADG01651.1.
GU723789 mRNA. Translation: ADG01652.1.
GU723790 mRNA. Translation: ADG01653.1.
GU723791 mRNA. Translation: ADG01654.1.
GU723866 Genomic DNA. Translation: ADG01729.1.
GU723867 Genomic DNA. Translation: ADG01730.1.
GU723870 Genomic DNA. Translation: ADG01731.1.
GU723871 Genomic DNA. Translation: ADG01732.1.
GU723872 Genomic DNA. Translation: ADG01733.1.
GU723873 Genomic DNA. Translation: ADG01734.1.
GU723874 Genomic DNA. Translation: ADG01735.1.
GU723875 Genomic DNA. Translation: ADG01736.1.
GU723876 Genomic DNA. Translation: ADG01737.1.
GU723877 Genomic DNA. Translation: ADG01738.1.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC
Protein Spotlight

Do it yourself - Issue 128 of May 2011

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF692484 Genomic DNA. Translation: ABU54597.1 .
EF692485 Genomic DNA. Translation: ABU54598.1 .
EF692488 mRNA. Translation: ABU54595.1 .
EF692489 mRNA. Translation: ABU54596.1 .
GU723782 mRNA. Translation: ADG01645.1 .
GU723783 mRNA. Translation: ADG01646.1 .
GU723784 mRNA. Translation: ADG01647.1 .
GU723785 mRNA. Translation: ADG01648.1 .
GU723786 mRNA. Translation: ADG01649.1 .
GU723787 mRNA. Translation: ADG01650.1 .
GU723788 mRNA. Translation: ADG01651.1 .
GU723789 mRNA. Translation: ADG01652.1 .
GU723790 mRNA. Translation: ADG01653.1 .
GU723791 mRNA. Translation: ADG01654.1 .
GU723866 Genomic DNA. Translation: ADG01729.1 .
GU723867 Genomic DNA. Translation: ADG01730.1 .
GU723870 Genomic DNA. Translation: ADG01731.1 .
GU723871 Genomic DNA. Translation: ADG01732.1 .
GU723872 Genomic DNA. Translation: ADG01733.1 .
GU723873 Genomic DNA. Translation: ADG01734.1 .
GU723874 Genomic DNA. Translation: ADG01735.1 .
GU723875 Genomic DNA. Translation: ADG01736.1 .
GU723876 Genomic DNA. Translation: ADG01737.1 .
GU723877 Genomic DNA. Translation: ADG01738.1 .

3D structure databases

ProteinModelPortali P0DH86.
SMRi P0DH86. Positions 33-161, 508-804.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P0DH86.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
2.90.10.10. 1 hit.
InterProi IPR001480. Bulb-type_lectin_dom.
IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR011009. Kinase-like_dom.
IPR013227. PAN-2_domain.
IPR003609. Pan_app.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR022126. S-locus_recpt_kinase.
IPR021820. S-locus_recpt_kinase_C.
IPR000858. S_locus_glycoprot.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR024171. SRK_like_kinase.
[Graphical view ]
Pfami PF01453. B_lectin. 1 hit.
PF11883. DUF3403. 1 hit.
PF12398. DUF3660. 1 hit.
PF08276. PAN_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF00954. S_locus_glycop. 1 hit.
[Graphical view ]
PIRSFi PIRSF000641. SRK. 1 hit.
SMARTi SM00108. B_lectin. 1 hit.
SM00181. EGF. 1 hit.
SM00473. PAN_AP. 1 hit.
[Graphical view ]
SUPFAMi SSF51110. SSF51110. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEi PS50927. BULB_LECTIN. 1 hit.
PS50948. PAN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Independent origins of self-compatibility in Arabidopsis thaliana."
    Shimizu K.K., Shimizu-Inatsugi R., Tsuchimatsu T., Purugganan M.D.
    Mol. Ecol. 17:704-714(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT THR-373.
    Strain: cv. Nok-0 and cv. Pog-0.
  2. "Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene."
    Tsuchimatsu T., Suwabe K., Shimizu-Inatsugi R., Isokawa S., Pavlidis P., Stadler T., Suzuki G., Takayama S., Watanabe M., Shimizu K.K.
    Nature 464:1342-1346(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS ASN-45; TYR-269; THR-278; LYS-415; ALA-420; SER-438; ILE-562; ARG-612; ASP-630; GLN-676 AND 829-SER--SER-837 DEL, FUNCTION.
    Strain: cv. Ca-0, cv. Co, cv. Di-1, cv. Fi-1, cv. Ge-1, cv. Gie, cv. Old-1, cv. Uk-3, cv. Wassilewskija and cv. Wei-1.
  3. "Natural variation in expression of self-incompatibility in Arabidopsis thaliana: implications for the evolution of selfing."
    Nasrallah M.E., Liu P., Sherman-Broyles S., Boggs N.A., Nasrallah J.B.
    Proc. Natl. Acad. Sci. U.S.A. 101:16070-16074(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "S locus genes and the evolution of self-fertility in Arabidopsis thaliana."
    Sherman-Broyles S., Boggs N., Farkas A., Liu P., Vrebalov J., Nasrallah M.E., Nasrallah J.B.
    Plant Cell 19:94-106(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REVIEW.
  5. Cited for: FUNCTION.

Entry informationi

Entry nameiSRK_ARATH
AccessioniPrimary (citable) accession number: P0DH86
Secondary accession number(s): B0F2A9
, B0F2B0, D6NTN9, D6NTP0, D6NTP1, D6NTP2, D6NTP6, D6NTP7, D6NTP8, O81904
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 19, 2011
Last modified: October 29, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Has been shown to be a pseudogene in cv. Columbia (AC P0DH87) due to a frameshift mutation that introduces a premature stop codon in this strain. The sequence shown is from strains cv. Pog-0 and cv. Wei-1.Curated

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3