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P0DG61 (SYW_STRPQ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:SPs1854
OrganismStreptococcus pyogenes serotype M3 (strain SSI-1) [Complete proteome] [HAMAP]
Taxonomic identifier193567 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence BAC64949.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000411620

Regions

Motif12 – 209"HIGH" region HAMAP-Rule MF_00140
Motif202 – 2065"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2051ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P0DG61 [UniParc].

Last modified July 27, 2011. Version 1.
Checksum: 5518E2E1E0757157

FASTA34038,373
        10         20         30         40         50         60 
MTKPIILTGD RPTGKLHLGH YVGSLKNRVF LQNENKYKMF VFLADQQALT DHAKESELIQ 

        70         80         90        100        110        120 
ESIGNVALDY LSVGLDPKQS TIFIQSQIPE LAELSMYYMN LVSLARLERN PTVKTEIAQK 

       130        140        150        160        170        180 
GFGESIPSGF LVYPVSQAAD ITAFKANLVP VGNDQKPMIE QTREIVRSFN HTYHTDCLVE 

       190        200        210        220        230        240 
PEGIYPENEK AGRLPGLDGN AKMSKSLGNG IYLSDDADTV RKKVMSMYTD PNHIKIEDPG 

       250        260        270        280        290        300 
QIEGNMVFHY LDIFARKEDQ ADIEAMKEHY QRGGLGDVKT KRYLLDILER ELAPIRERRL 

       310        320        330        340 
EYAKDMGEVF RMLQEGSQKA RTVAAKTLSE VKSAMGINYF 

« Hide

References

[1]"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution."
Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S.
Genome Res. 13:1042-1055(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SSI-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000034 Genomic DNA. Translation: BAC64949.1. Different initiation.
RefSeqNP_803116.2. NC_004606.1.

3D structure databases

ProteinModelPortalP0DG61.
SMRP0DG61. Positions 2-339.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC64949; BAC64949; BAC64949.
GeneID1065065.
KEGGsps:SPs1854.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000059941.
KOK01867.
OMAYGYGHAK.
OrthoDBEOG686NJQ.

Enzyme and pathway databases

BioCycSPYO193567:GHDO-1921-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_STRPQ
AccessionPrimary (citable) accession number: P0DG61
Secondary accession number(s): P67597, Q8NYZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: July 27, 2011
Last modified: February 19, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries