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P0DG33 (SYC_STRPQ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteine--tRNA ligase

EC=6.1.1.16
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS
Gene names
Name:cysS
Ordered Locus Names:SPs1673
OrganismStreptococcus pyogenes serotype M3 (strain SSI-1) [Complete proteome] [HAMAP]
Taxonomic identifier193567 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length447 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00041

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00041

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00041.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcysteinyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

cysteine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 447447Cysteine--tRNA ligase HAMAP-Rule MF_00041
PRO_0000411606

Regions

Motif30 – 4011"HIGH" region HAMAP-Rule MF_00041
Motif268 – 2725"KMSKS" region HAMAP-Rule MF_00041

Sites

Metal binding281Zinc By similarity
Metal binding2111Zinc By similarity
Metal binding2361Zinc By similarity
Metal binding2401Zinc By similarity
Binding site2711ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P0DG33 [UniParc].

Last modified July 27, 2011. Version 1.
Checksum: 5C892A47E3C5C297

FASTA44750,465
        10         20         30         40         50         60 
MIKIYDTMTR SLRKFVPLTE NTVNMYVCGP TVYNYIHIGN ARSAVAFDTI RRYFEYTGYQ 

        70         80         90        100        110        120 
VNYISNFTDI DDKIIKAATQ AGVSPKELSD RFIAAFIEDT KALGVKPATQ NPRVMDYIAE 

       130        140        150        160        170        180 
IISFVESLIE KDFAYEADGD VYFRVEKSEH YAKLANKTLS ELEVGASGRT DAETALKENP 

       190        200        210        220        230        240 
LDFALWKSAK AGEVSWDSPW GFGRPGWHIE CSVMATEILG DTIDIHGGGA DLEFPHHTNE 

       250        260        270        280        290        300 
IAQSEAKTGK TFANYWMHNG FVTVDNEKMS KSLGNFVTVH DMLQTVDGQV LRFFLATQQY 

       310        320        330        340        350        360 
RKPINFTEKA IHDAEINLKY LKNTLQQPLT ETADEQELKQ FVIAFQDAMD DDFNTANGIT 

       370        380        390        400        410        420 
VVFDMAKWIN SGSYTEPVKS AFEKMLAVFG IIFEEEVLEV DIEALIAKRQ EARANRDFAT 

       430        440 
ADAIRDQLAA QGIKLLDTKD GVRWLRD 

« Hide

References

[1]"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution."
Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S.
Genome Res. 13:1042-1055(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SSI-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000034 Genomic DNA. Translation: BAC64768.1.
RefSeqNP_802935.1. NC_004606.1.

3D structure databases

ProteinModelPortalP0DG33.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC64768; BAC64768; BAC64768.
GeneID1066349.
KEGGsps:SPs1673.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000245250.
KOK01883.
OMAQIDIHAG.
OrthoDBEOG6RVFXC.

Enzyme and pathway databases

BioCycSPYO193567:GHDO-1738-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00041. Cys_tRNA_synth.
InterProIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERPTHR10890. PTHR10890. 1 hit.
PfamPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSPR00983. TRNASYNTHCYS.
SMARTSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
TIGRFAMsTIGR00435. cysS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYC_STRPQ
AccessionPrimary (citable) accession number: P0DG33
Secondary accession number(s): Q8K5T7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: July 27, 2011
Last modified: February 19, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries