P0DG33 (SYC_STRPQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	SPs1673

Organism

Streptococcus pyogenes serotype M3 (strain SSI-1) [Complete proteome] [HAMAP]

Taxonomic identifier

193567 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pyogenes › Streptococcus pyogenes serotype M3

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Cysteine--tRNA ligase HAMAP-Rule MF_00041		PRO_0000411606
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP-Rule MF_00041
	Motif	268 – 272	5	"KMSKS" region HAMAP-Rule MF_00041
Sites
	Metal binding	28	1	Zinc By similarity
	Metal binding	211	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Metal binding	240	1	Zinc By similarity
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0DG33 [UniParc]. Last modified July 27, 2011. Version 1. Checksum: 5C892A47E3C5C297	FASTA	447	50,465
	10 20 30 40 50 60 MIKIYDTMTR SLRKFVPLTE NTVNMYVCGP TVYNYIHIGN ARSAVAFDTI RRYFEYTGYQ 70 80 90 100 110 120 VNYISNFTDI DDKIIKAATQ AGVSPKELSD RFIAAFIEDT KALGVKPATQ NPRVMDYIAE 130 140 150 160 170 180 IISFVESLIE KDFAYEADGD VYFRVEKSEH YAKLANKTLS ELEVGASGRT DAETALKENP 190 200 210 220 230 240 LDFALWKSAK AGEVSWDSPW GFGRPGWHIE CSVMATEILG DTIDIHGGGA DLEFPHHTNE 250 260 270 280 290 300 IAQSEAKTGK TFANYWMHNG FVTVDNEKMS KSLGNFVTVH DMLQTVDGQV LRFFLATQQY 310 320 330 340 350 360 RKPINFTEKA IHDAEINLKY LKNTLQQPLT ETADEQELKQ FVIAFQDAMD DDFNTANGIT 370 380 390 400 410 420 VVFDMAKWIN SGSYTEPVKS AFEKMLAVFG IIFEEEVLEV DIEALIAKRQ EARANRDFAT 430 440 ADAIRDQLAA QGIKLLDTKD GVRWLRD

References

[1]

"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution." Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S. Genome Res. 13:1042-1055(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SSI-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000034 Genomic DNA. Translation: BAC64768.1.
RefSeq	NP_802935.1. NC_004606.1.
3D structure databases
ProteinModelPortal	P0DG33.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC64768; BAC64768; BAC64768.
GeneID	1066349.
KEGG	sps:SPs1673.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000245250.
KO	K01883.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00041. Cys_tRNA_synth.
InterPro	IPR015803. Cys-tRNA-ligase. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR10890. PTHR10890. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_STRPQ

Accession

Primary (citable) accession number: P0DG33
Secondary accession number(s): Q8K5T7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program