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P0DG21 (TRMD_STRPQ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase

EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:SPs1280
OrganismStreptococcus pyogenes serotype M3 (strain SSI-1) [Complete proteome] [HAMAP]
Taxonomic identifier193567 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP-Rule MF_00xxx

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_00xxx

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00xxx

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00xxx.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine(37)-N(1))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243tRNA (guanine-N(1)-)-methyltransferase HAMAP-Rule MF_00xxx
PRO_0000411600

Regions

Region127 – 1326S-adenosyl-L-methionine binding By similarity

Sites

Binding site1081S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P0DG21 [UniParc].

Last modified July 27, 2011. Version 1.
Checksum: 25D0A4B59777CFA1

FASTA24327,979
        10         20         30         40         50         60 
MKIDILTLFP EMFAPLEHSI VGKAKEKGLL DIHYHNFRDY AEKARHVDDE PYGGGQGMLL 

        70         80         90        100        110        120 
RAQPIFDTIE QIEAKKPRII LLDPAGKPFT QAYAEELALE EELIFICGHY EGYDERIKTL 

       130        140        150        160        170        180 
VTDEISLGDF VLTGGELAAM TMVDATVRLI PQVLGKESSH QDDSFSSGLL EYPQYTRPYD 

       190        200        210        220        230        240 
YRGMTVPDVL MSGHHERIRL WRLEESLRKT YLRRPDLLER YDFSEEERKL LDKIKEALGQ 


GED 

« Hide

References

[1]"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution."
Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S.
Genome Res. 13:1042-1055(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SSI-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000034 Genomic DNA. Translation: BAC64375.1.
RefSeqNP_802542.1. NC_004606.1.

3D structure databases

ProteinModelPortalP0DG21.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC64375; BAC64375; BAC64375.
GeneID1065324.
KEGGsps:SPs1280.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000016242.
KOK00554.
OMADWAKEDE.
OrthoDBEOG6J48RZ.

Enzyme and pathway databases

BioCycSPYO193567:GHDO-1339-MONOMER.

Family and domain databases

Gene3D1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPMF_00605. TrmD.
InterProIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMSSF75217. SSF75217. 1 hit.
TIGRFAMsTIGR00088. trmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_STRPQ
AccessionPrimary (citable) accession number: P0DG21
Secondary accession number(s): Q8K7X3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: July 27, 2011
Last modified: June 11, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families