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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44S-adenosyl-L-methionineUniRule annotation1
Binding sitei69S-adenosyl-L-methionineUniRule annotation1
Binding sitei96S-adenosyl-L-methionineUniRule annotation1
Active sitei118By similarity1
Binding sitei118S-adenosyl-L-methionineUniRule annotation1
Binding sitei122SubstrateUniRule annotation1
Binding sitei154SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:SpyM3_1500
OrganismiStreptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315)
Taxonomic identifieri198466 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000564 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001714061 – 211tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST211

Structurei

3D structure databases

ProteinModelPortaliP0DG18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 129Interaction with RNAUniRule annotation6
Regioni191 – 194Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0DG18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVRKRKGAE EHLANNPHYV ILNPEDAKGR WHDVFGNDRP IHIEVGSGKG
60 70 80 90 100
GFITGMALKN PDINYIGIDI QLSVLSYALD KVLASEVPNV KLLRVDGSSL
110 120 130 140 150
TNYFEDGEVD MMYLNFSDPW PKTKHEKRRL TYKDFLDTYK RILPEHGEIH
160 170 180 190 200
FKTDNRGLFE YSLASFSQYG MTLRQIWLDL HASNYEGNVM TEYEEKFSNK
210
GQVIYRVEAN F
Length:211
Mass (Da):24,455
Last modified:July 27, 2011 - v1
Checksum:i09C90C702C222E6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014074 Genomic DNA. Translation: AAM80107.1.
RefSeqiWP_002983387.1. NC_004070.1.

Genome annotation databases

EnsemblBacteriaiAAM80107; AAM80107; SpyM3_1500.
KEGGispg:SpyM3_1500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014074 Genomic DNA. Translation: AAM80107.1.
RefSeqiWP_002983387.1. NC_004070.1.

3D structure databases

ProteinModelPortaliP0DG18.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM80107; AAM80107; SpyM3_1500.
KEGGispg:SpyM3_1500.

Phylogenomic databases

HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_STRP3
AccessioniPrimary (citable) accession number: P0DG18
Secondary accession number(s): P58090, P67505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.