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P0DG18 (TRMB_STRP3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:SpyM3_1500
OrganismStreptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315) [Complete proteome] [HAMAP]
Taxonomic identifier198466 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus ›

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 211211tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171406

Regions

Region124 – 1296Interaction with RNA Potential
Region191 – 1944Substrate binding Potential

Sites

Binding site441S-adenosyl-L-methionine By similarity
Binding site691S-adenosyl-L-methionine By similarity
Binding site961S-adenosyl-L-methionine By similarity
Binding site1181S-adenosyl-L-methionine By similarity
Binding site1221Substrate By similarity
Binding site1541Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
P0DG18 [UniParc].

Last modified July 27, 2011. Version 1.
Checksum: 09C90C702C222E6D

FASTA21124,455
        10         20         30         40         50         60 
MRVRKRKGAE EHLANNPHYV ILNPEDAKGR WHDVFGNDRP IHIEVGSGKG GFITGMALKN 

        70         80         90        100        110        120 
PDINYIGIDI QLSVLSYALD KVLASEVPNV KLLRVDGSSL TNYFEDGEVD MMYLNFSDPW 

       130        140        150        160        170        180 
PKTKHEKRRL TYKDFLDTYK RILPEHGEIH FKTDNRGLFE YSLASFSQYG MTLRQIWLDL 

       190        200        210 
HASNYEGNVM TEYEEKFSNK GQVIYRVEAN F

« Hide

References

[1]"Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the high-virulence phenotype, and clone emergence."
Beres S.B., Sylva G.L., Barbian K.D., Lei B., Hoff J.S., Mammarella N.D., Liu M.-Y., Smoot J.C., Porcella S.F., Parkins L.D., Campbell D.S., Smith T.M., McCormick J.K., Leung D.Y.M., Schlievert P.M., Musser J.M.
Proc. Natl. Acad. Sci. U.S.A. 99:10078-10083(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-595 / MGAS315.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE014074 Genomic DNA. Translation: AAM80107.1.
RefSeqNP_665304.1. NC_004070.1.

3D structure databases

ProteinModelPortalP0DG18.
SMRP0DG18. Positions 6-211.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM80107; AAM80107; SpyM3_1500.
GeneID1009815.
KEGGspg:SpyM3_1500.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000251689.
KOK03439.
OMARLRNKPW.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_STRP3
AccessionPrimary (citable) accession number: P0DG18
Secondary accession number(s): P58090, P67505
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: July 27, 2011
Last modified: April 3, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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