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P0DF15 (RNC_STRPQ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 3

EC=3.1.26.3
Alternative name(s):
Ribonuclease III
Short name=RNase III
Gene names
Name:rnc
Synonyms:acpA
Ordered Locus Names:SPs1478
OrganismStreptococcus pyogenes serotype M3 (strain SSI-1) [Complete proteome] [HAMAP]
Taxonomic identifier193567 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104

Cofactor

Mg2+ By similarity. HAMAP-Rule MF_00104

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00104

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00104.

Sequence similarities

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 RNase III domain.

Ontologies

Keywords
   Biological processmRNA processing
rRNA processing
tRNA processing
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
RNA-binding
rRNA-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

rRNA catabolic process

Inferred from electronic annotation. Source: InterPro

rRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

rRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonuclease III activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 230230Ribonuclease 3 HAMAP-Rule MF_00104
PRO_0000411547

Regions

Domain1 – 134134RNase III
Domain160 – 22970DRBM

Sites

Active site511 Potential
Active site1231 By similarity
Metal binding471Magnesium By similarity
Metal binding1201Magnesium By similarity
Metal binding1231Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
P0DF15 [UniParc].

Last modified July 27, 2011. Version 1.
Checksum: 20076A9055B59B4E

FASTA23025,848
        10         20         30         40         50         60 
MKQLEELLST SFDIQFNDLT LLETAFTHTS YANEHRLLNV SHNERLEFLG DAVLQLIISE 

        70         80         90        100        110        120 
YLFAKYPKKT EGDMSKLRSM IVREESLAGF SRFCSFDAYI KLGKGEEKSG GRRRDTILGD 

       130        140        150        160        170        180 
LFEAFLGALL LDKGIDAVRR FLKQVMIPQV EKGNFERVKD YKTCLQEFLQ TKGDVAIDYQ 

       190        200        210        220        230 
VISEKGPAHA KQFEVSIVVN GAVLSKGLGK SKKLAEQDAA KNALAQLSEV 

« Hide

References

[1]"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution."
Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S.
Genome Res. 13:1042-1055(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SSI-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000034 Genomic DNA. Translation: BAC64573.1.
RefSeqNP_802740.1. NC_004606.1.

3D structure databases

ProteinModelPortalP0DF15.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC64573; BAC64573; BAC64573.
GeneID1066821.
KEGGsps:SPs1478.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000246808.
KOK03685.
OMAYGFQEAR.
OrthoDBEOG6T1WVS.

Enzyme and pathway databases

BioCycSPYO193567:GHDO-1538-MONOMER.

Family and domain databases

Gene3D1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPMF_00104. RNase_III.
InterProIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMSSF69065. SSF69065. 1 hit.
TIGRFAMsTIGR02191. RNaseIII. 1 hit.
PROSITEPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNC_STRPQ
AccessionPrimary (citable) accession number: P0DF15
Secondary accession number(s): P66671, Q9A105
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: July 27, 2011
Last modified: May 14, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families