P0DB96 (KITH_STRP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	SpyM3_0798

Organism

Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315) [Complete proteome] [HAMAP]

Taxonomic identifier

198466 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pyogenes › Streptococcus pyogenes serotype M3

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.
Sequence caution	The sequence AAM79405.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000175035
Regions
	Nucleotide binding	9 – 16	8	ATP By similarity
	Nucleotide binding	85 – 88	4	ATP By similarity
Sites
	Active site	86	1	Proton acceptor Potential
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	180	1	Zinc By similarity
	Metal binding	183	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0DB96 [UniParc]. Last modified July 27, 2011. Version 1. Checksum: BF99B031909B821E	FASTA	189	21,559
	10 20 30 40 50 60 MAQLYYKYGT MNSGKTIEIL KVAHNYEEQG KPVVIMTSAL DTRDGFGIVS SRIGMRREAI 70 80 90 100 110 120 PISNDMDIFT FIAQLEEKPY CVLIDESQFL SKQNVYDLAR VVDELNVPVM AFGLKNDFQN 130 140 150 160 170 180 NLFEGSKHLL LLADKIDEIK TICQYCSKKA TMVLRIENGK PVYEGDQIQI GGNETYIPVC RKHYFNPEI

References

[1]

"Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the high-virulence phenotype, and clone emergence." Beres S.B., Sylva G.L., Barbian K.D., Lei B., Hoff J.S., Mammarella N.D., Liu M.-Y., Smoot J.C., Porcella S.F., Parkins L.D., Campbell D.S., Smith T.M., McCormick J.K., Leung D.Y.M., Schlievert P.M., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:10078-10083(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-595 / MGAS315.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014074 Genomic DNA. Translation: AAM79405.1. Different initiation.
RefSeq	NP_664602.1. NC_004070.1.
3D structure databases
ProteinModelPortal	P0DB96.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM79405; AAM79405; SpyM3_0798.
GeneID	1009112.
KEGG	spg:SpyM3_0798.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000076391.
KO	K00857.
OMA	FRYGAMN.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_STRP3

Accession

Primary (citable) accession number: P0DB96
Secondary accession number(s): Q878R8, Q8K7I3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program