P0DB37 (TRMFO_STRPQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
EC=2.1.1.74

Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Synonyms:	gid
Ordered Locus Names:	SPs1022

Organism

Streptococcus pyogenes serotype M3 (strain SSI-1) [Complete proteome] [HAMAP]

Taxonomic identifier

193567 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pyogenes › Streptococcus pyogenes serotype M3

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP-Rule MF_01037
Catalytic activity	5,10-methylenetetrahydrofolate + uridine54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluridine54 in tRNA + FAD. HAMAP-Rule MF_01037
Cofactor	FAD By similarity. HAMAP-Rule MF_01037
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01037.
Sequence similarities	Belongs to the MnmG family. TrmFO subfamily.
Sequence caution	The sequence BAC64117.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Inferred from electronic annotation. Source: HAMAP flavin adenine dinucleotide binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP-Rule MF_01037		PRO_0000411356
Regions
	Nucleotide binding	13 – 18	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0DB37 [UniParc]. Last modified July 27, 2011. Version 1. Checksum: A7185DA471557AAA	FASTA	448	49,448
	10 20 30 40 50 60 MSQSTATYIN VIGAGLAGSE AAYQIAKRGI PVKLYEMRGV KATPQHKTTN FAELVCSNSF 70 80 90 100 110 120 RGDSLTNAVG LLKEEMRRLD SIIMRNGEAN RVPAGGAMAV DREGYAKSVT AELENHPLIE 130 140 150 160 170 180 VIRDEITEIP NDAITVIATG PLTSDALAEK IHAVNGGDGF YFYDAAAPII DKSTIDMSKV 190 200 210 220 230 240 YLKSRYDKGE AAYLNCPMTK EEFMAFHEAL TTAEEAPLNS FEKEKYFEGC MPIEVMAKRG 250 260 270 280 290 300 IKTMLYGPMK PVGLEYPDDY TGPRDGEFKT PYAVVQLRQD NAAGSLYNIV GFQTHLKWGE 310 320 330 340 350 360 QKRVFQMIPG LENAEFVRYG VMHRNSYMDS PNLLTETFQS RSNPNLLFAG QMTGVEGYVE 370 380 390 400 410 420 SAASGLVAGI NAARLFKREE ALIFPQTTAI GSLPHYVTHA DSKHFQPMNV NFGIIKELEG 430 440 PRIRDKKERY EAIASRALAD LDTCLASL

References

[1]

"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution." Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S. Genome Res. 13:1042-1055(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SSI-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000034 Genomic DNA. Translation: BAC64117.1. Different initiation.
RefSeq	NP_802284.1. NC_004606.1.
3D structure databases
ProteinModelPortal	P0DB37.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC64117; BAC64117; BAC64117.
GeneID	1065454.
KEGG	sps:SPs1022.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000252054.
KO	K04094.
Enzyme and pathway databases
BioCyc	SPYO193567:GHDO-1078-MONOMER.
Family and domain databases
HAMAP	MF_01037. TrmFO.
InterPro	IPR004417. Folate-dep_Ribothymidyl_synth. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_STRPQ

Accession

Primary (citable) accession number: P0DB37
Secondary accession number(s): Q8K7G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program