##gff-version 3
P0CY08	UniProtKB	Chain	1	210	.	.	.	ID=PRO_0000049186;Note=Mating-type protein ALPHA2	
P0CY08	UniProtKB	DNA binding	129	191	.	.	.	Note=Homeobox%3B TALE-type;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00108	
P0CY08	UniProtKB	Region	1	102	.	.	.	Note=N-terminal domain	
P0CY08	UniProtKB	Region	103	128	.	.	.	Note=Flexible linker	
P0CY08	UniProtKB	Region	190	210	.	.	.	Note=C-terminal tail	
P0CY08	UniProtKB	Modified residue	1	1	.	.	.	Note=N-acetylmethionine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20110468;Dbxref=PMID:20110468	
P0CY08	UniProtKB	Mutagenesis	4	4	.	.	.	Note=Reduces the ability of ALPHA2 to repress transcription%2C but binds normally to DNA and MCM1. I->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7995523;Dbxref=PMID:7995523	
P0CY08	UniProtKB	Mutagenesis	9	9	.	.	.	Note=Reduces the ability of ALPHA2 to repress transcription%2C but binds normally to DNA and MCM1. L->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7995523;Dbxref=PMID:7995523	
P0CY08	UniProtKB	Mutagenesis	10	10	.	.	.	Note=Reduces the ability of ALPHA2 to repress transcription%2C but binds normally to DNA and MCM1. Disrupts interaction with TUP1. L->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7995523;Dbxref=PMID:7995523	
P0CY08	UniProtKB	Mutagenesis	71	71	.	.	.	Note=Reduces the ability of alpha2 to repress transcription%2C but binds normally to DNA and MCM1. E->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7995523;Dbxref=PMID:7995523	
P0CY08	UniProtKB	Mutagenesis	114	114	.	.	.	Note=Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:2887035;Dbxref=PMID:2887035	
P0CY08	UniProtKB	Mutagenesis	115	115	.	.	.	Note=Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. V->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:2887035;Dbxref=PMID:2887035	
P0CY08	UniProtKB	Mutagenesis	116	116	.	.	.	Note=Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:2887035;Dbxref=PMID:2887035	
P0CY08	UniProtKB	Mutagenesis	117	117	.	.	.	Note=Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:2887035;Dbxref=PMID:2887035	
P0CY08	UniProtKB	Mutagenesis	118	118	.	.	.	Note=Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. V->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:2887035;Dbxref=PMID:2887035	
P0CY08	UniProtKB	Mutagenesis	119	119	.	.	.	Note=Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. V->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:2887035;Dbxref=PMID:2887035	
P0CY08	UniProtKB	Mutagenesis	120	120	.	.	.	Note=Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:2887035;Dbxref=PMID:2887035	
P0CY08	UniProtKB	Mutagenesis	173	173	.	.	.	Note=Reduces the ability of ALPHA2/MCM1 to repress a-specific genes. Disrupts interaction with SSN6. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10759558;Dbxref=PMID:10759558	
P0CY08	UniProtKB	Mutagenesis	181	181	.	.	.	Note=In ALPHA2-3A%3B defective in binding DNA alone or in complex with MCM1%2C but binds DNA normally in complex with A1%3B when associated with A-182 and A-185. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12121651;Dbxref=PMID:12121651	
P0CY08	UniProtKB	Mutagenesis	182	182	.	.	.	Note=In ALPHA2-3A%3B defective in binding DNA alone or in complex with MCM1%2C but binds DNA normally in complex with A1%3B when associated with A-181 and A-185. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12121651;Dbxref=PMID:12121651	
P0CY08	UniProtKB	Mutagenesis	185	185	.	.	.	Note=In ALPHA2-3A%3B defective in binding DNA alone or in complex with MCM1%2C but binds DNA normally in complex with A1%3B when associated with A-181 and A-182. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12121651;Dbxref=PMID:12121651	
P0CY08	UniProtKB	Mutagenesis	192	192	.	.	.	Note=Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes. I->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7569974;Dbxref=PMID:7569974	
P0CY08	UniProtKB	Mutagenesis	196	196	.	.	.	Note=Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes. L->A%2CS;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:2851482,ECO:0000269|PubMed:7569974;Dbxref=PMID:2851482,PMID:7569974	
P0CY08	UniProtKB	Mutagenesis	199	199	.	.	.	Note=Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7569974;Dbxref=PMID:7569974	
P0CY08	UniProtKB	Mutagenesis	200	200	.	.	.	Note=Disrupts the ability of A1/ALPHA2 to repress haploid-specific genes. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7569974;Dbxref=PMID:7569974	
P0CY08	UniProtKB	Sequence conflict	56	56	.	.	.	Note=G->L;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P0CY08	UniProtKB	Sequence conflict	150	150	.	.	.	Note=K->KK;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P0CY08	UniProtKB	Sequence conflict	154	154	.	.	.	Note=N->S;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P0CY08	UniProtKB	Beta strand	116	120	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1MNM	
P0CY08	UniProtKB	Beta strand	125	129	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1MNM	
P0CY08	UniProtKB	Helix	138	150	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1K61	
P0CY08	UniProtKB	Turn	151	153	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1K61	
P0CY08	UniProtKB	Helix	159	169	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1K61	
P0CY08	UniProtKB	Helix	173	188	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1K61	
P0CY08	UniProtKB	Helix	194	197	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1LE8	
P0CY08	UniProtKB	Beta strand	200	202	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1LE8