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P0CY06

- MTAL1_YEAST

UniProt

P0CY06 - MTAL1_YEAST

Protein

Mating-type protein ALPHA1

Gene

MATALPHA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 20 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional coactivator that, in alpha-cells, binds cooperatively with MCM1 and STE12 to a DNA sequence termed the QP' element, to activate the transcription of alpha-specific genes.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi88 – 14457Alpha boxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding, bending Source: SGD
    2. sequence-specific DNA binding Source: InterPro
    3. transcription coactivator activity Source: SGD

    GO - Biological processi

    1. positive regulation of mating-type specific transcription, DNA-templated Source: InterPro
    2. regulation of mating-type specific transcription, DNA-templated Source: SGD
    3. regulation of transcription from RNA polymerase II promoter Source: SGD
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29352-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mating-type protein ALPHA1
    Short name:
    MATalpha1 protein
    Alternative name(s):
    Alpha-1 activator
    Gene namesi
    Name:MATALPHA1
    Synonyms:ALPHA-1, MAT1A, MATAL1
    Ordered Locus Names:YCR040W
    ORF Names:YCR40W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR040w.
    SGDiS000000636. MATALPHA1.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 175175Mating-type protein ALPHA1PRO_0000096610Add
    BLAST

    Expressioni

    Developmental stagei

    Only present in alpha-cells.

    Inductioni

    Repressed in a/alpha diploid cells by A1/ALPHA2.

    Interactioni

    Subunit structurei

    Binds DNA with a high specificity in complex with an MCM1 dimer. Interacts with STE12.2 Publications

    Protein-protein interaction databases

    BioGridi30923. 6 interactions.
    31023. 5 interactions.
    IntActiP0CY06. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CY06.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MATALPHA1 family.PROSITE-ProRule annotation
    Contains 1 alpha box DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOGENOMiHOG000057084.
    OrthoDBiEOG7B3209.

    Family and domain databases

    InterProiIPR017931. Alpha_box.
    IPR006856. MAT_alpha1.
    IPR016325. Transcrpt_activator_MAT_Alpha1.
    [Graphical view]
    PfamiPF04769. MAT_Alpha1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001863. Transcrpt_activ_MAT_Alpha1. 1 hit.
    PROSITEiPS51325. ALPHA_BOX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0CY06-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFTSKPAFKI KNKASKSYRN TAVSKKLKEK RLAEHVRPSC FNIIRPLKKD    50
    IQIPVPSSRF LNKIQIHRIA SGSQNTQFRQ FNKTSIKSSK KYLNSFMAFR 100
    AYYSQFGSGV KQNVLSSLLA EEWHADKMQH GIWDYFAQQY NFINPGFGFV 150
    EWLTNNYAEV RGDGYWEDVF VHLAL 175
    Length:175
    Mass (Da):20,413
    Last modified:June 28, 2011 - v1
    Checksum:i697199A7686D19C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00060 Genomic DNA. Translation: AAA34763.1.
    X63853 Genomic DNA. Translation: CAA45336.1.
    X59720 Genomic DNA. Translation: CAA42401.1.
    AY692791 Genomic DNA. Translation: AAT92810.1.
    BK006937 Genomic DNA. Translation: DAA07422.1.
    PIRiS19397. JFBYA1.
    RefSeqiNP_009867.1. NM_001178707.1.
    NP_009869.3. NM_001178754.1.

    Genome annotation databases

    EnsemblFungiiYCL066W; YCL066W; YCL066W.
    YCR040W; YCR040W; YCR040W.
    GeneIDi850293.
    850407.
    KEGGisce:YCL066W.
    sce:YCR040W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L00060 Genomic DNA. Translation: AAA34763.1 .
    X63853 Genomic DNA. Translation: CAA45336.1 .
    X59720 Genomic DNA. Translation: CAA42401.1 .
    AY692791 Genomic DNA. Translation: AAT92810.1 .
    BK006937 Genomic DNA. Translation: DAA07422.1 .
    PIRi S19397. JFBYA1.
    RefSeqi NP_009867.1. NM_001178707.1.
    NP_009869.3. NM_001178754.1.

    3D structure databases

    ProteinModelPortali P0CY06.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30923. 6 interactions.
    31023. 5 interactions.
    IntActi P0CY06. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCL066W ; YCL066W ; YCL066W .
    YCR040W ; YCR040W ; YCR040W .
    GeneIDi 850293.
    850407.
    KEGGi sce:YCL066W.
    sce:YCR040W.

    Organism-specific databases

    CYGDi YCR040w.
    SGDi S000000636. MATALPHA1.

    Phylogenomic databases

    HOGENOMi HOG000057084.
    OrthoDBi EOG7B3209.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29352-MONOMER.

    Miscellaneous databases

    NextBioi 965661.

    Family and domain databases

    InterProi IPR017931. Alpha_box.
    IPR006856. MAT_alpha1.
    IPR016325. Transcrpt_activator_MAT_Alpha1.
    [Graphical view ]
    Pfami PF04769. MAT_Alpha1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001863. Transcrpt_activ_MAT_Alpha1. 1 hit.
    PROSITEi PS51325. ALPHA_BOX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Physical analysis of mating-type loci in Saccharomyces cerevisiae."
      Nasmyth K.A., Tatchell K., Hall B.D., Astell C., Smith M.
      Cold Spring Harb. Symp. Quant. Biol. 45:961-981(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The MAT locus revisited within a 9.8 kb fragment of chromosome III containing BUD5 and two new open reading frames."
      Jacquet M., Buhler J.-M., Iborra F., Francingues-Gaillard M.-C., Soustelle C.
      Yeast 7:881-888(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Transcription of alpha-specific genes in Saccharomyces cerevisiae: DNA sequence requirements for activity of the coregulator alpha 1."
      Hagen D.C., Bruhn L., Westby C.A., Sprague G.F. Jr.
      Mol. Cell. Biol. 13:6866-6875(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING SPECIFICITY.
    7. "The DNA binding and oligomerization domain of MCM1 is sufficient for its interaction with other regulatory proteins."
      Primig M., Winkler H., Ammerer G.
      EMBO J. 10:4209-4218(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MCM1.
    8. "Coupling of cell identity to signal response in yeast: interaction between the alpha-1 and STE12 proteins."
      Yuan Y.-L.O., Stroke I., Fields S.
      Genes Dev. 7:1584-1597(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH STE12.
    9. "Molecular mechanisms of cell-type determination in budding yeast."
      Johnson A.D.
      Curr. Opin. Genet. Dev. 5:552-558(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN MATING-TYPE REGULATION.

    Entry informationi

    Entry nameiMTAL1_YEAST
    AccessioniPrimary (citable) accession number: P0CY06
    Secondary accession number(s): D6VQV3, P01365
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 20 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are three genetic loci for mating type genes in S.cerevisiae. MAT is the expression locus that determines the mating type of the cell, whereas HML (containing HMLALPHA1 and HMLALPHA2) and HMR (containing HMRA1 and HMRA2) represent silenced repositories of mating type information. The mating type is determined by the MAT locus, which contains either a copy of HML or of HMR. Diploid cells are usually heterozygous for the MAT locus.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3