P0CY06 (MTAL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mating-type protein ALPHA1
Short name=MATalpha1 protein

Alternative name(s):
Alpha-1 activator

Gene names

Name:	MATALPHA1
Synonyms:	ALPHA-1, MAT1A, MATAL1
Ordered Locus Names:	YCR040W
ORF Names:	YCR40W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional coactivator that, in alpha-cells, binds cooperatively with MCM1 and STE12 to a DNA sequence termed the QP' element, to activate the transcription of alpha-specific genes. Ref.9
Subunit structure	Binds DNA with a high specificity in complex with an MCM1 dimer. Interacts with STE12. Ref.7 Ref.8
Subcellular location	Nucleus Potential.
Developmental stage	Only present in alpha-cells.
Induction	Repressed in a/alpha diploid cells by A1/ALPHA2.
Miscellaneous	There are three genetic loci for mating type genes in S.cerevisiae. MAT is the expression locus that determines the mating type of the cell, whereas HML (containing HMLALPHA1 and HMLALPHA2) and HMR (containing HMRA1 and HMRA2) represent silenced repositories of mating type information. The mating type is determined by the MAT locus, which contains either a copy of HML or of HMR. Diploid cells are usually heterozygous for the MAT locus.
Sequence similarities	Belongs to the MATALPHA1 family. Contains 1 alpha box DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of mating-type specific transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro regulation of mating-type specific transcription, DNA-dependent Inferred from mutant phenotype PubMed 6337727. Source: SGD regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 6337727. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred by curator PubMed 6337727. Source: SGD
Molecular_function	DNA binding, bending Inferred from direct assay PubMed 15118075. Source: SGD sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription coactivator activity Inferred from mutant phenotype PubMed 6337727. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Mating-type protein ALPHA1		PRO_0000096610
Regions
	DNA binding	88 – 144	57	Alpha box Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P0CY06 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 697199A7686D19C0	FASTA	175	20,413
	10 20 30 40 50 60 MFTSKPAFKI KNKASKSYRN TAVSKKLKEK RLAEHVRPSC FNIIRPLKKD IQIPVPSSRF 70 80 90 100 110 120 LNKIQIHRIA SGSQNTQFRQ FNKTSIKSSK KYLNSFMAFR AYYSQFGSGV KQNVLSSLLA 130 140 150 160 170 EEWHADKMQH GIWDYFAQQY NFINPGFGFV EWLTNNYAEV RGDGYWEDVF VHLAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Physical analysis of mating-type loci in Saccharomyces cerevisiae." Nasmyth K.A., Tatchell K., Hall B.D., Astell C., Smith M. Cold Spring Harb. Symp. Quant. Biol. 45:961-981(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The MAT locus revisited within a 9.8 kb fragment of chromosome III containing BUD5 and two new open reading frames." Jacquet M., Buhler J.-M., Iborra F., Francingues-Gaillard M.-C., Soustelle C. Yeast 7:881-888(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Transcription of alpha-specific genes in Saccharomyces cerevisiae: DNA sequence requirements for activity of the coregulator alpha 1." Hagen D.C., Bruhn L., Westby C.A., Sprague G.F. Jr. Mol. Cell. Biol. 13:6866-6875(1993) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING SPECIFICITY.
[7]	"The DNA binding and oligomerization domain of MCM1 is sufficient for its interaction with other regulatory proteins." Primig M., Winkler H., Ammerer G. EMBO J. 10:4209-4218(1991) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MCM1.
[8]	"Coupling of cell identity to signal response in yeast: interaction between the alpha-1 and STE12 proteins." Yuan Y.-L.O., Stroke I., Fields S. Genes Dev. 7:1584-1597(1993) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH STE12.
[9]	"Molecular mechanisms of cell-type determination in budding yeast." Johnson A.D. Curr. Opin. Genet. Dev. 5:552-558(1995) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN MATING-TYPE REGULATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L00060 Genomic DNA. Translation: AAA34763.1. X63853 Genomic DNA. Translation: CAA45336.1. X59720 Genomic DNA. Translation: CAA42401.1. AY692791 Genomic DNA. Translation: AAT92810.1. BK006937 Genomic DNA. Translation: DAA07422.1.
PIR	JFBYA1. S19397.
RefSeq	NP_009867.1. NM_001178707.1. NP_009869.3. NM_001178754.1.
3D structure databases
ProteinModelPortal	P0CY06.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0CY06. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCL066W; YCL066W; YCL066W. YCR040W; YCR040W; YCR040W.
GeneID	850293. 850407.
KEGG	sce:YCL066W. sce:YCR040W.
Organism-specific databases
CYGD	YCR040w.
SGD	S000000636. MATALPHA1.
Phylogenomic databases
HOGENOM	HOG000057084.
Gene expression databases
ArrayExpress	P0CY06.
GermOnline	YCR040W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017931. Alpha_box. IPR006856. MAT_alpha1. IPR016325. Transcrpt_activator_MAT_Alpha1. [Graphical view]
Pfam	PF04769. MAT_Alpha1. 1 hit. [Graphical view]
PIRSF	PIRSF001863. Transcrpt_activ_MAT_Alpha1. 1 hit.
PROSITE	PS51325. ALPHA_BOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	965661.

Entry information

Entry name

MTAL1_YEAST

Accession

Primary (citable) accession number: P0CY06
Secondary accession number(s): D6VQV3, P01365

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program