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Protein

Mating-type protein ALPHA1

Gene

MATALPHA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional coactivator that, in alpha-cells, binds cooperatively with MCM1 and STE12 to a DNA sequence termed the QP' element, to activate the transcription of alpha-specific genes.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi88 – 144Alpha boxPROSITE-ProRule annotationAdd BLAST57

GO - Molecular functioni

  • DNA binding, bending Source: SGD
  • transcription coactivator activity Source: SGD

GO - Biological processi

  • positive regulation of mating-type specific transcription, DNA-templated Source: InterPro
  • regulation of mating-type specific transcription, DNA-templated Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29352-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mating-type protein ALPHA1
Short name:
MATalpha1 protein
Alternative name(s):
Alpha-1 activator
Gene namesi
Name:MATALPHA1
Synonyms:ALPHA-1, MAT1A, MATAL1
Ordered Locus Names:YCR040W
ORF Names:YCR40W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

SGDiS000000636. MATALPHA1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000966101 – 175Mating-type protein ALPHA1Add BLAST175

Proteomic databases

PRIDEiP0CY06.

Expressioni

Developmental stagei

Only present in alpha-cells.

Inductioni

Repressed in a/alpha diploid cells by A1/ALPHA2.

Interactioni

Subunit structurei

Binds DNA with a high specificity in complex with an MCM1 dimer. Interacts with STE12.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
STE12P135742EBI-10438,EBI-18264

Protein-protein interaction databases

BioGridi30923. 6 interactors.
31023. 4 interactors.
IntActiP0CY06. 4 interactors.

Structurei

3D structure databases

ProteinModelPortaliP0CY06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MATALPHA1 family.PROSITE-ProRule annotation
Contains 1 alpha box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000057084.
InParanoidiP0CY06.
OrthoDBiEOG092C4Z9U.

Family and domain databases

InterProiIPR006856. MATalpha_HMGbox.
[Graphical view]
PfamiPF04769. MATalpha_HMGbox. 1 hit.
[Graphical view]
PROSITEiPS51325. ALPHA_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CY06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTSKPAFKI KNKASKSYRN TAVSKKLKEK RLAEHVRPSC FNIIRPLKKD
60 70 80 90 100
IQIPVPSSRF LNKIQIHRIA SGSQNTQFRQ FNKTSIKSSK KYLNSFMAFR
110 120 130 140 150
AYYSQFGSGV KQNVLSSLLA EEWHADKMQH GIWDYFAQQY NFINPGFGFV
160 170
EWLTNNYAEV RGDGYWEDVF VHLAL
Length:175
Mass (Da):20,413
Last modified:June 28, 2011 - v1
Checksum:i697199A7686D19C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00060 Genomic DNA. Translation: AAA34763.1.
X63853 Genomic DNA. Translation: CAA45336.1.
X59720 Genomic DNA. Translation: CAA42401.1.
AY692791 Genomic DNA. Translation: AAT92810.1.
BK006937 Genomic DNA. Translation: DAA07422.1.
PIRiS19397. JFBYA1.
RefSeqiNP_009867.1. NM_001178707.1.
NP_009869.3. NM_001178754.1.

Genome annotation databases

EnsemblFungiiCAA42401; CAA42401; CAA42401.
YCL066W; YCL066W; YCL066W.
YCR040W; YCR040W; YCR040W.
GeneIDi850293.
850407.
KEGGisce:YCL066W.
sce:YCR040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L00060 Genomic DNA. Translation: AAA34763.1.
X63853 Genomic DNA. Translation: CAA45336.1.
X59720 Genomic DNA. Translation: CAA42401.1.
AY692791 Genomic DNA. Translation: AAT92810.1.
BK006937 Genomic DNA. Translation: DAA07422.1.
PIRiS19397. JFBYA1.
RefSeqiNP_009867.1. NM_001178707.1.
NP_009869.3. NM_001178754.1.

3D structure databases

ProteinModelPortaliP0CY06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30923. 6 interactors.
31023. 4 interactors.
IntActiP0CY06. 4 interactors.

Proteomic databases

PRIDEiP0CY06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42401; CAA42401; CAA42401.
YCL066W; YCL066W; YCL066W.
YCR040W; YCR040W; YCR040W.
GeneIDi850293.
850407.
KEGGisce:YCL066W.
sce:YCR040W.

Organism-specific databases

SGDiS000000636. MATALPHA1.

Phylogenomic databases

HOGENOMiHOG000057084.
InParanoidiP0CY06.
OrthoDBiEOG092C4Z9U.

Enzyme and pathway databases

BioCyciYEAST:G3O-29352-MONOMER.

Miscellaneous databases

PROiP0CY06.

Family and domain databases

InterProiIPR006856. MATalpha_HMGbox.
[Graphical view]
PfamiPF04769. MATalpha_HMGbox. 1 hit.
[Graphical view]
PROSITEiPS51325. ALPHA_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTAL1_YEAST
AccessioniPrimary (citable) accession number: P0CY06
Secondary accession number(s): D6VQV3, P01365
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 30, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

There are three genetic loci for mating type genes in S.cerevisiae. MAT is the expression locus that determines the mating type of the cell, whereas HML (containing HMLALPHA1 and HMLALPHA2) and HMR (containing HMRA1 and HMRA2) represent silenced repositories of mating type information. The mating type is determined by the MAT locus, which contains either a copy of HML or of HMR. Diploid cells are usually heterozygous for the MAT locus.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.