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P0CY06

- MTAL1_YEAST

UniProt

P0CY06 - MTAL1_YEAST

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Protein

Mating-type protein ALPHA1

Gene

MATALPHA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional coactivator that, in alpha-cells, binds cooperatively with MCM1 and STE12 to a DNA sequence termed the QP' element, to activate the transcription of alpha-specific genes.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi88 – 14457Alpha boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding, bending Source: SGD
  2. sequence-specific DNA binding Source: InterPro
  3. transcription coactivator activity Source: SGD

GO - Biological processi

  1. positive regulation of mating-type specific transcription, DNA-templated Source: InterPro
  2. regulation of mating-type specific transcription, DNA-templated Source: SGD
  3. regulation of transcription from RNA polymerase II promoter Source: SGD
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29352-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mating-type protein ALPHA1
Short name:
MATalpha1 protein
Alternative name(s):
Alpha-1 activator
Gene namesi
Name:MATALPHA1
Synonyms:ALPHA-1, MAT1A, MATAL1
Ordered Locus Names:YCR040W
ORF Names:YCR40W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR040w.
SGDiS000000636. MATALPHA1.

Subcellular locationi

Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 175175Mating-type protein ALPHA1PRO_0000096610Add
BLAST

Expressioni

Developmental stagei

Only present in alpha-cells.

Inductioni

Repressed in a/alpha diploid cells by A1/ALPHA2.

Interactioni

Subunit structurei

Binds DNA with a high specificity in complex with an MCM1 dimer. Interacts with STE12.2 Publications

Protein-protein interaction databases

BioGridi30923. 6 interactions.
31023. 5 interactions.
IntActiP0CY06. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP0CY06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MATALPHA1 family.PROSITE-ProRule annotation
Contains 1 alpha box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000057084.
InParanoidiP0CY06.
OrthoDBiEOG7B3209.

Family and domain databases

InterProiIPR017931. Alpha_box.
IPR006856. MAT_alpha1.
IPR016325. Transcrpt_activator_MAT_Alpha1.
[Graphical view]
PfamiPF04769. MAT_Alpha1. 1 hit.
[Graphical view]
PIRSFiPIRSF001863. Transcrpt_activ_MAT_Alpha1. 1 hit.
PROSITEiPS51325. ALPHA_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CY06-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFTSKPAFKI KNKASKSYRN TAVSKKLKEK RLAEHVRPSC FNIIRPLKKD
60 70 80 90 100
IQIPVPSSRF LNKIQIHRIA SGSQNTQFRQ FNKTSIKSSK KYLNSFMAFR
110 120 130 140 150
AYYSQFGSGV KQNVLSSLLA EEWHADKMQH GIWDYFAQQY NFINPGFGFV
160 170
EWLTNNYAEV RGDGYWEDVF VHLAL
Length:175
Mass (Da):20,413
Last modified:June 28, 2011 - v1
Checksum:i697199A7686D19C0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L00060 Genomic DNA. Translation: AAA34763.1.
X63853 Genomic DNA. Translation: CAA45336.1.
X59720 Genomic DNA. Translation: CAA42401.1.
AY692791 Genomic DNA. Translation: AAT92810.1.
BK006937 Genomic DNA. Translation: DAA07422.1.
PIRiS19397. JFBYA1.
RefSeqiNP_009867.1. NM_001178707.1.
NP_009869.3. NM_001178754.1.

Genome annotation databases

EnsemblFungiiYCL066W; YCL066W; YCL066W.
YCR040W; YCR040W; YCR040W.
GeneIDi850293.
850407.
KEGGisce:YCL066W.
sce:YCR040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L00060 Genomic DNA. Translation: AAA34763.1 .
X63853 Genomic DNA. Translation: CAA45336.1 .
X59720 Genomic DNA. Translation: CAA42401.1 .
AY692791 Genomic DNA. Translation: AAT92810.1 .
BK006937 Genomic DNA. Translation: DAA07422.1 .
PIRi S19397. JFBYA1.
RefSeqi NP_009867.1. NM_001178707.1.
NP_009869.3. NM_001178754.1.

3D structure databases

ProteinModelPortali P0CY06.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30923. 6 interactions.
31023. 5 interactions.
IntActi P0CY06. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCL066W ; YCL066W ; YCL066W .
YCR040W ; YCR040W ; YCR040W .
GeneIDi 850293.
850407.
KEGGi sce:YCL066W.
sce:YCR040W.

Organism-specific databases

CYGDi YCR040w.
SGDi S000000636. MATALPHA1.

Phylogenomic databases

HOGENOMi HOG000057084.
InParanoidi P0CY06.
OrthoDBi EOG7B3209.

Enzyme and pathway databases

BioCyci YEAST:G3O-29352-MONOMER.

Miscellaneous databases

NextBioi 965661.

Family and domain databases

InterProi IPR017931. Alpha_box.
IPR006856. MAT_alpha1.
IPR016325. Transcrpt_activator_MAT_Alpha1.
[Graphical view ]
Pfami PF04769. MAT_Alpha1. 1 hit.
[Graphical view ]
PIRSFi PIRSF001863. Transcrpt_activ_MAT_Alpha1. 1 hit.
PROSITEi PS51325. ALPHA_BOX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Physical analysis of mating-type loci in Saccharomyces cerevisiae."
    Nasmyth K.A., Tatchell K., Hall B.D., Astell C., Smith M.
    Cold Spring Harb. Symp. Quant. Biol. 45:961-981(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The MAT locus revisited within a 9.8 kb fragment of chromosome III containing BUD5 and two new open reading frames."
    Jacquet M., Buhler J.-M., Iborra F., Francingues-Gaillard M.-C., Soustelle C.
    Yeast 7:881-888(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Transcription of alpha-specific genes in Saccharomyces cerevisiae: DNA sequence requirements for activity of the coregulator alpha 1."
    Hagen D.C., Bruhn L., Westby C.A., Sprague G.F. Jr.
    Mol. Cell. Biol. 13:6866-6875(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING SPECIFICITY.
  7. "The DNA binding and oligomerization domain of MCM1 is sufficient for its interaction with other regulatory proteins."
    Primig M., Winkler H., Ammerer G.
    EMBO J. 10:4209-4218(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MCM1.
  8. "Coupling of cell identity to signal response in yeast: interaction between the alpha-1 and STE12 proteins."
    Yuan Y.-L.O., Stroke I., Fields S.
    Genes Dev. 7:1584-1597(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH STE12.
  9. "Molecular mechanisms of cell-type determination in budding yeast."
    Johnson A.D.
    Curr. Opin. Genet. Dev. 5:552-558(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MATING-TYPE REGULATION.

Entry informationi

Entry nameiMTAL1_YEAST
AccessioniPrimary (citable) accession number: P0CY06
Secondary accession number(s): D6VQV3, P01365
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: October 29, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

There are three genetic loci for mating type genes in S.cerevisiae. MAT is the expression locus that determines the mating type of the cell, whereas HML (containing HMLALPHA1 and HMLALPHA2) and HMR (containing HMRA1 and HMRA2) represent silenced repositories of mating type information. The mating type is determined by the MAT locus, which contains either a copy of HML or of HMR. Diploid cells are usually heterozygous for the MAT locus.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3