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P0CY06 (MTAL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mating-type protein ALPHA1

Short name=MATalpha1 protein
Alternative name(s):
Alpha-1 activator
Gene names
Name:MATALPHA1
Synonyms:ALPHA-1, MAT1A, MATAL1
Ordered Locus Names:YCR040W
ORF Names:YCR40W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length175 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional coactivator that, in alpha-cells, binds cooperatively with MCM1 and STE12 to a DNA sequence termed the QP' element, to activate the transcription of alpha-specific genes. Ref.9

Subunit structure

Binds DNA with a high specificity in complex with an MCM1 dimer. Interacts with STE12. Ref.7 Ref.8

Subcellular location

Nucleus Potential.

Developmental stage

Only present in alpha-cells.

Induction

Repressed in a/alpha diploid cells by A1/ALPHA2.

Miscellaneous

There are three genetic loci for mating type genes in S.cerevisiae. MAT is the expression locus that determines the mating type of the cell, whereas HML (containing HMLALPHA1 and HMLALPHA2) and HMR (containing HMRA1 and HMRA2) represent silenced repositories of mating type information. The mating type is determined by the MAT locus, which contains either a copy of HML or of HMR. Diploid cells are usually heterozygous for the MAT locus.

Sequence similarities

Belongs to the MATALPHA1 family.

Contains 1 alpha box DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 175175Mating-type protein ALPHA1
PRO_0000096610

Regions

DNA binding88 – 14457Alpha box Ref.6

Sequences

Sequence LengthMass (Da)Tools
P0CY06 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 697199A7686D19C0

FASTA17520,413
        10         20         30         40         50         60 
MFTSKPAFKI KNKASKSYRN TAVSKKLKEK RLAEHVRPSC FNIIRPLKKD IQIPVPSSRF 

        70         80         90        100        110        120 
LNKIQIHRIA SGSQNTQFRQ FNKTSIKSSK KYLNSFMAFR AYYSQFGSGV KQNVLSSLLA 

       130        140        150        160        170 
EEWHADKMQH GIWDYFAQQY NFINPGFGFV EWLTNNYAEV RGDGYWEDVF VHLAL 

« Hide

References

« Hide 'large scale' references
[1]"Physical analysis of mating-type loci in Saccharomyces cerevisiae."
Nasmyth K.A., Tatchell K., Hall B.D., Astell C., Smith M.
Cold Spring Harb. Symp. Quant. Biol. 45:961-981(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The MAT locus revisited within a 9.8 kb fragment of chromosome III containing BUD5 and two new open reading frames."
Jacquet M., Buhler J.-M., Iborra F., Francingues-Gaillard M.-C., Soustelle C.
Yeast 7:881-888(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Transcription of alpha-specific genes in Saccharomyces cerevisiae: DNA sequence requirements for activity of the coregulator alpha 1."
Hagen D.C., Bruhn L., Westby C.A., Sprague G.F. Jr.
Mol. Cell. Biol. 13:6866-6875(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING SPECIFICITY.
[7]"The DNA binding and oligomerization domain of MCM1 is sufficient for its interaction with other regulatory proteins."
Primig M., Winkler H., Ammerer G.
EMBO J. 10:4209-4218(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MCM1.
[8]"Coupling of cell identity to signal response in yeast: interaction between the alpha-1 and STE12 proteins."
Yuan Y.-L.O., Stroke I., Fields S.
Genes Dev. 7:1584-1597(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH STE12.
[9]"Molecular mechanisms of cell-type determination in budding yeast."
Johnson A.D.
Curr. Opin. Genet. Dev. 5:552-558(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN MATING-TYPE REGULATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L00060 Genomic DNA. Translation: AAA34763.1.
X63853 Genomic DNA. Translation: CAA45336.1.
X59720 Genomic DNA. Translation: CAA42401.1.
AY692791 Genomic DNA. Translation: AAT92810.1.
BK006937 Genomic DNA. Translation: DAA07422.1.
PIRJFBYA1. S19397.
RefSeqNP_009867.1. NM_001178707.1.
NP_009869.3. NM_001178754.1.

3D structure databases

ProteinModelPortalP0CY06.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid30923. 6 interactions.
31023. 5 interactions.
IntActP0CY06. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCL066W; YCL066W; YCL066W.
YCR040W; YCR040W; YCR040W.
GeneID850293.
850407.
KEGGsce:YCL066W.
sce:YCR040W.

Organism-specific databases

CYGDYCR040w.
SGDS000000636. MATALPHA1.

Phylogenomic databases

HOGENOMHOG000057084.
OrthoDBEOG7B3209.

Enzyme and pathway databases

BioCycYEAST:G3O-29352-MONOMER.

Family and domain databases

InterProIPR017931. Alpha_box.
IPR006856. MAT_alpha1.
IPR016325. Transcrpt_activator_MAT_Alpha1.
[Graphical view]
PfamPF04769. MAT_Alpha1. 1 hit.
[Graphical view]
PIRSFPIRSF001863. Transcrpt_activ_MAT_Alpha1. 1 hit.
PROSITEPS51325. ALPHA_BOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio965661.

Entry information

Entry nameMTAL1_YEAST
AccessionPrimary (citable) accession number: P0CY06
Secondary accession number(s): D6VQV3, P01365
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: July 9, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families