Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

60S ribosomal protein L35-A

Gene

RPL35A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29576-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L35-A
Gene namesi
Name:RPL35A
Synonyms:SOS1
Ordered Locus Names:YDL191W
ORF Names:D1249
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDL191w.
SGDiS000002350. RPL35A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 12011960S ribosomal protein L35-APRO_0000130552Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131Phosphoserine1 Publication
Modified residuei50 – 501Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CX84.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi31854. 101 interactions.
31925. 134 interactions.
IntActiP0CX84. 1 interaction.
MINTiMINT-8285624.
STRINGi4932.YDL191W.

Structurei

Secondary structure

1
120
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 94Combined sources
Helixi14 – 3522Combined sources
Helixi43 – 6826Combined sources
Turni69 – 724Combined sources
Helixi78 – 803Combined sources
Helixi86 – 894Combined sources
Helixi94 – 985Combined sources
Helixi102 – 1109Combined sources
Beta strandi116 – 1183Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-Y2-60[»]
2WW9electron microscopy8.60N1-120[»]
2WWAelectron microscopy8.90N1-120[»]
2WWBelectron microscopy6.48N1-120[»]
3J6Xelectron microscopy6.10751-120[»]
3J6Yelectron microscopy6.10751-120[»]
3J77electron microscopy6.20851-120[»]
3J78electron microscopy6.30851-120[»]
4U3MX-ray3.00O5/o52-120[»]
4U3NX-ray3.20O5/o52-120[»]
4U3UX-ray2.90O5/o52-120[»]
4U4NX-ray3.10O5/o52-120[»]
4U4OX-ray3.60O5/o52-120[»]
4U4QX-ray3.00O5/o52-120[»]
4U4RX-ray2.80O5/o52-120[»]
4U4UX-ray3.00O5/o52-120[»]
4U4YX-ray3.20O5/o52-120[»]
4U4ZX-ray3.10O5/o52-120[»]
4U50X-ray3.20O5/o52-120[»]
4U51X-ray3.20O5/o52-120[»]
4U52X-ray3.00O5/o52-120[»]
4U53X-ray3.30O5/o52-120[»]
4U55X-ray3.20O5/o52-120[»]
4U56X-ray3.45O5/o52-120[»]
4U6FX-ray3.10O5/o52-120[»]
4V4Belectron microscopy11.70BX1-120[»]
4V6Ielectron microscopy8.80Bc1-120[»]
4V7Felectron microscopy8.70Z1-120[»]
4V7RX-ray4.00Bc/Dc1-120[»]
4V88X-ray3.00Bh/Dh1-120[»]
4V8Telectron microscopy8.10h1-120[»]
4V91electron microscopy3.70h1-120[»]
ProteinModelPortaliP0CX84.
SMRiP0CX84. Positions 1-69.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX84.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L29P family.Curated

Phylogenomic databases

InParanoidiP0CX84.
KOiK02918.
OrthoDBiEOG71P2Q2.

Family and domain databases

Gene3Di1.10.287.310. 1 hit.
HAMAPiMF_00374. Ribosomal_L29.
InterProiIPR001854. Ribosomal_L29.
IPR018254. Ribosomal_L29_CS.
[Graphical view]
PfamiPF00831. Ribosomal_L29. 1 hit.
[Graphical view]
SUPFAMiSSF46561. SSF46561. 1 hit.
TIGRFAMsiTIGR00012. L29. 1 hit.
PROSITEiPS00579. RIBOSOMAL_L29. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS
60 70 80 90 100
IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALRR ALTKFEASQV
110 120
TEKQRKKQIA FPQRKYAIKA
Length:120
Mass (Da):13,910
Last modified:June 28, 2011 - v1
Checksum:iD8AC9DF157AD2AD6
GO

Mass spectrometryi

Molecular mass is 13769.991 Da from positions 2 - 120. Determined by ESI. Monoisotopic mass.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82913 Genomic DNA. Translation: AAA34492.1.
X83276 Genomic DNA. Translation: CAA58256.1.
Z74239 Genomic DNA. Translation: CAA98768.1.
BK006938 Genomic DNA. Translation: DAA11722.1.
PIRiS30770.
RefSeqiNP_010090.1. NM_001180251.2.
NP_010145.1. NM_001180196.2.

Genome annotation databases

EnsemblFungiiYDL136W; YDL136W; YDL136W.
YDL191W; YDL191W; YDL191W.
GeneIDi851336.
851419.
KEGGisce:YDL136W.
sce:YDL191W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82913 Genomic DNA. Translation: AAA34492.1.
X83276 Genomic DNA. Translation: CAA58256.1.
Z74239 Genomic DNA. Translation: CAA98768.1.
BK006938 Genomic DNA. Translation: DAA11722.1.
PIRiS30770.
RefSeqiNP_010090.1. NM_001180251.2.
NP_010145.1. NM_001180196.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-Y2-60[»]
2WW9electron microscopy8.60N1-120[»]
2WWAelectron microscopy8.90N1-120[»]
2WWBelectron microscopy6.48N1-120[»]
3J6Xelectron microscopy6.10751-120[»]
3J6Yelectron microscopy6.10751-120[»]
3J77electron microscopy6.20851-120[»]
3J78electron microscopy6.30851-120[»]
4U3MX-ray3.00O5/o52-120[»]
4U3NX-ray3.20O5/o52-120[»]
4U3UX-ray2.90O5/o52-120[»]
4U4NX-ray3.10O5/o52-120[»]
4U4OX-ray3.60O5/o52-120[»]
4U4QX-ray3.00O5/o52-120[»]
4U4RX-ray2.80O5/o52-120[»]
4U4UX-ray3.00O5/o52-120[»]
4U4YX-ray3.20O5/o52-120[»]
4U4ZX-ray3.10O5/o52-120[»]
4U50X-ray3.20O5/o52-120[»]
4U51X-ray3.20O5/o52-120[»]
4U52X-ray3.00O5/o52-120[»]
4U53X-ray3.30O5/o52-120[»]
4U55X-ray3.20O5/o52-120[»]
4U56X-ray3.45O5/o52-120[»]
4U6FX-ray3.10O5/o52-120[»]
4V4Belectron microscopy11.70BX1-120[»]
4V6Ielectron microscopy8.80Bc1-120[»]
4V7Felectron microscopy8.70Z1-120[»]
4V7RX-ray4.00Bc/Dc1-120[»]
4V88X-ray3.00Bh/Dh1-120[»]
4V8Telectron microscopy8.10h1-120[»]
4V91electron microscopy3.70h1-120[»]
ProteinModelPortaliP0CX84.
SMRiP0CX84. Positions 1-69.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31854. 101 interactions.
31925. 134 interactions.
IntActiP0CX84. 1 interaction.
MINTiMINT-8285624.
STRINGi4932.YDL191W.

Proteomic databases

MaxQBiP0CX84.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL136W; YDL136W; YDL136W.
YDL191W; YDL191W; YDL191W.
GeneIDi851336.
851419.
KEGGisce:YDL136W.
sce:YDL191W.

Organism-specific databases

CYGDiYDL191w.
SGDiS000002350. RPL35A.

Phylogenomic databases

InParanoidiP0CX84.
KOiK02918.
OrthoDBiEOG71P2Q2.

Enzyme and pathway databases

BioCyciYEAST:G3O-29576-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

EvolutionaryTraceiP0CX84.
NextBioi968405.
PROiP0CX84.

Family and domain databases

Gene3Di1.10.287.310. 1 hit.
HAMAPiMF_00374. Ribosomal_L29.
InterProiIPR001854. Ribosomal_L29.
IPR018254. Ribosomal_L29_CS.
[Graphical view]
PfamiPF00831. Ribosomal_L29. 1 hit.
[Graphical view]
SUPFAMiSSF46561. SSF46561. 1 hit.
TIGRFAMsiTIGR00012. L29. 1 hit.
PROSITEiPS00579. RIBOSOMAL_L29. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast SIS1 protein, a DnaJ homolog, is required for the initiation of translation."
    Zhong T., Arndt K.T.
    Cell 73:1175-1186(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein."
    Verhasselt P., Voet M., Mathys J., Volckaert G.
    Yeast 12:1065-1070(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  6. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
    Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
    Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13 AND SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 2-60, ELECTRON MICROSCOPY.
  11. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
  12. "Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome."
    Becker T., Bhushan S., Jarasch A., Armache J.P., Funes S., Jossinet F., Gumbart J., Mielke T., Berninghausen O., Schulten K., Westhof E., Gilmore R., Mandon E.C., Beckmann R.
    Science 326:1369-1373(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.60 ANGSTROMS).
  13. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.80 ANGSTROMS).
  14. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.00 ANGSTROMS).
  15. Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS).
  16. "Cryo-EM structures of Arx1 and maturation factors Rei1 and Jjj1 bound to the 60S ribosomal subunit."
    Greber B.J., Boehringer D., Montellese C., Ban N.
    Nat. Struct. Mol. Biol. 19:1228-1233(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.10 ANGSTROMS).

Entry informationi

Entry nameiRL35A_YEAST
AccessioniPrimary (citable) accession number: P0CX84
Secondary accession number(s): D6VRG2, P39741, P39930
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: June 24, 2015
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 2 genes for L35 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.