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Protein

60S ribosomal protein L35-A

Gene

RPL35A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

There are 2 genes for uL29 in yeast.Curated

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29576-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L35-A1 Publication
Alternative name(s):
Large ribosomal subunit protein uL29-A1 Publication
Gene namesi
Name:RPL35A1 Publication
Synonyms:SOS1
Ordered Locus Names:YDL191W
ORF Names:D1249
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

SGDiS000002350 RPL35A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001305522 – 12060S ribosomal protein L35-AAdd BLAST119

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineCombined sources1
Modified residuei50PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CX84
PaxDbiP0CX84
PRIDEiP0CX84

PTM databases

iPTMnetiP0CX84

Expressioni

Gene expression databases

ExpressionAtlasiP0CX84 differential

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). uL29 is associated with the polypeptide exit tunnel (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi31854, 240 interactors
31925, 191 interactors
IntActiP0CX84, 1 interactor
MINTiP0CX84
STRINGi4932.YDL191W

Structurei

Secondary structure

1120
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 10Combined sources5
Helixi14 – 33Combined sources20
Turni34 – 37Combined sources4
Helixi43 – 69Combined sources27
Turni70 – 72Combined sources3
Turni78 – 80Combined sources3
Turni87 – 89Combined sources3
Helixi94 – 98Combined sources5
Helixi102 – 110Combined sources9
Beta strandi116 – 118Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-Y2-60[»]
2WW9electron microscopy8.60N1-120[»]
2WWAelectron microscopy8.90N1-120[»]
2WWBelectron microscopy6.48N1-120[»]
3J6Xelectron microscopy6.10751-120[»]
3J6Yelectron microscopy6.10751-120[»]
3J77electron microscopy6.20851-120[»]
3J78electron microscopy6.30851-120[»]
3JCTelectron microscopy3.08h1-120[»]
4U3MX-ray3.00O5/o52-120[»]
4U3NX-ray3.20O5/o52-120[»]
4U3UX-ray2.90O5/o52-120[»]
4U4NX-ray3.10O5/o52-120[»]
4U4OX-ray3.60O5/o52-120[»]
4U4QX-ray3.00O5/o52-120[»]
4U4RX-ray2.80O5/o52-120[»]
4U4UX-ray3.00O5/o52-120[»]
4U4YX-ray3.20O5/o52-120[»]
4U4ZX-ray3.10O5/o52-120[»]
4U50X-ray3.20O5/o52-120[»]
4U51X-ray3.20O5/o52-120[»]
4U52X-ray3.00O5/o52-120[»]
4U53X-ray3.30O5/o52-120[»]
4U55X-ray3.20O5/o52-120[»]
4U56X-ray3.45O5/o52-120[»]
4U6FX-ray3.10O5/o52-120[»]
4V4Belectron microscopy11.70BX1-120[»]
4V6Ielectron microscopy8.80Bc1-120[»]
4V7Felectron microscopy8.70Z1-120[»]
4V7RX-ray4.00Bc/Dc1-120[»]
4V88X-ray3.00Bh/Dh1-120[»]
4V8Telectron microscopy8.10h1-120[»]
4V91electron microscopy3.70h1-120[»]
5APNelectron microscopy3.91h1-120[»]
5APOelectron microscopy3.41h1-120[»]
5DATX-ray3.15O5/o52-120[»]
5DC3X-ray3.25O5/o52-120[»]
5DGEX-ray3.45O5/o52-120[»]
5DGFX-ray3.30O5/o52-120[»]
5DGVX-ray3.10O5/o52-120[»]
5FCIX-ray3.40O5/o52-120[»]
5FCJX-ray3.10O5/o52-120[»]
5FL8electron microscopy9.50h1-120[»]
5GAKelectron microscopy3.88j1-120[»]
5H4Pelectron microscopy3.07h1-120[»]
5I4LX-ray3.10O5/o52-120[»]
5JCSelectron microscopy9.50h1-120[»]
5JUOelectron microscopy4.00MA1-120[»]
5JUPelectron microscopy3.50MA1-120[»]
5JUSelectron microscopy4.20MA1-120[»]
5JUTelectron microscopy4.00MA1-120[»]
5JUUelectron microscopy4.00MA1-120[»]
5LYBX-ray3.25O5/o52-120[»]
5M1Jelectron microscopy3.30h52-120[»]
5MC6electron microscopy3.80BP1-120[»]
5MEIX-ray3.50AI/DJ2-120[»]
5NDGX-ray3.70O5/o52-120[»]
5NDVX-ray3.30O5/o52-120[»]
5NDWX-ray3.70O5/o52-120[»]
5OBMX-ray3.40O5/o52-120[»]
5ON6X-ray3.10AI/DJ2-120[»]
5T62electron microscopy3.30u1-120[»]
5T6Relectron microscopy4.50u1-120[»]
5TBWX-ray3.00AI/DJ2-120[»]
5TGAX-ray3.30O5/o52-120[»]
5TGMX-ray3.50O5/o52-120[»]
5Z3Gelectron microscopy3.65l1-120[»]
6C0Felectron microscopy3.70h1-120[»]
6ELZelectron microscopy3.30h1-120[»]
6EM1electron microscopy3.60h1-120[»]
6EM3electron microscopy3.20h1-120[»]
6EM4electron microscopy4.10h1-120[»]
6EM5electron microscopy4.30h1-120[»]
6FT6electron microscopy3.90h1-120[»]
ProteinModelPortaliP0CX84
SMRiP0CX84
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX84

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP0CX84
KOiK02918
OMAiTVINAKQ
OrthoDBiEOG092C5O2N

Family and domain databases

HAMAPiMF_00374 Ribosomal_L29, 1 hit
InterProiView protein in InterPro
IPR001854 Ribosomal_L29/L35
IPR036049 Ribosomal_L29/L35_sf
IPR018254 Ribosomal_L29_CS
PfamiView protein in Pfam
PF00831 Ribosomal_L29, 1 hit
SUPFAMiSSF46561 SSF46561, 1 hit
TIGRFAMsiTIGR00012 L29, 1 hit
PROSITEiView protein in PROSITE
PS00579 RIBOSOMAL_L29, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS
60 70 80 90 100
IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALRR ALTKFEASQV
110 120
TEKQRKKQIA FPQRKYAIKA
Length:120
Mass (Da):13,910
Last modified:June 28, 2011 - v1
Checksum:iD8AC9DF157AD2AD6
GO

Mass spectrometryi

Molecular mass is 13769.991 Da from positions 2 - 120. Determined by ESI. Monoisotopic mass.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82913 Genomic DNA Translation: AAA34492.1
X83276 Genomic DNA Translation: CAA58256.1
Z74239 Genomic DNA Translation: CAA98768.1
BK006938 Genomic DNA Translation: DAA11722.1
PIRiS30770
RefSeqiNP_010090.1, NM_001180251.2
NP_010145.1, NM_001180196.2

Genome annotation databases

EnsemblFungiiYDL136W; YDL136W; YDL136W
YDL191W; YDL191W; YDL191W
GeneIDi851336
851419
KEGGisce:YDL136W
sce:YDL191W

Similar proteinsi

Entry informationi

Entry nameiRL35A_YEAST
AccessioniPrimary (citable) accession number: P0CX84
Secondary accession number(s): D6VRG2, P39741, P39930
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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