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Protein

60S ribosomal protein L35-A

Gene

RPL35A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

There are 2 genes for uL29 in yeast.Curated

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29576-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L35-A1 Publication
Alternative name(s):
Large ribosomal subunit protein uL29-A1 Publication
Gene namesi
Name:RPL35A1 Publication
Synonyms:SOS1
Ordered Locus Names:YDL191W
ORF Names:D1249
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

SGDiS000002350. RPL35A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD
  • preribosome, large subunit precursor Source: SGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001305522 – 12060S ribosomal protein L35-AAdd BLAST119

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineCombined sources1
Modified residuei50PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CX84.
PRIDEiP0CX84.

PTM databases

iPTMnetiP0CX84.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX84. differential.

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). uL29 is associated with the polypeptide exit tunnel (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi31854. 235 interactors.
31925. 187 interactors.
IntActiP0CX84. 1 interactor.
MINTiMINT-8285624.
STRINGi4932.YDL191W.

Structurei

Secondary structure

1120
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 9Combined sources4
Helixi14 – 35Combined sources22
Helixi43 – 68Combined sources26
Turni69 – 72Combined sources4
Helixi78 – 80Combined sources3
Helixi86 – 89Combined sources4
Helixi94 – 98Combined sources5
Helixi102 – 110Combined sources9
Beta strandi116 – 118Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-Y2-60[»]
2WW9electron microscopy8.60N1-120[»]
2WWAelectron microscopy8.90N1-120[»]
2WWBelectron microscopy6.48N1-120[»]
3J6Xelectron microscopy6.10751-120[»]
3J6Yelectron microscopy6.10751-120[»]
3J77electron microscopy6.20851-120[»]
3J78electron microscopy6.30851-120[»]
3JCTelectron microscopy3.08h1-120[»]
4U3MX-ray3.00O5/o52-120[»]
4U3NX-ray3.20O5/o52-120[»]
4U3UX-ray2.90O5/o52-120[»]
4U4NX-ray3.10O5/o52-120[»]
4U4OX-ray3.60O5/o52-120[»]
4U4QX-ray3.00O5/o52-120[»]
4U4RX-ray2.80O5/o52-120[»]
4U4UX-ray3.00O5/o52-120[»]
4U4YX-ray3.20O5/o52-120[»]
4U4ZX-ray3.10O5/o52-120[»]
4U50X-ray3.20O5/o52-120[»]
4U51X-ray3.20O5/o52-120[»]
4U52X-ray3.00O5/o52-120[»]
4U53X-ray3.30O5/o52-120[»]
4U55X-ray3.20O5/o52-120[»]
4U56X-ray3.45O5/o52-120[»]
4U6FX-ray3.10O5/o52-120[»]
4V4Belectron microscopy11.70BX1-120[»]
4V6Ielectron microscopy8.80Bc1-120[»]
4V7Felectron microscopy8.70Z1-120[»]
4V7RX-ray4.00Bc/Dc1-120[»]
4V88X-ray3.00Bh/Dh1-120[»]
4V8Telectron microscopy8.10h1-120[»]
4V91electron microscopy3.70h1-120[»]
5APNelectron microscopy3.91h1-120[»]
5APOelectron microscopy3.41h1-120[»]
5DATX-ray3.15O5/o52-120[»]
5DC3X-ray3.25O5/o52-120[»]
5DGEX-ray3.45O5/o52-120[»]
5DGFX-ray3.30O5/o52-120[»]
5DGVX-ray3.10O5/o52-120[»]
5FCIX-ray3.40O5/o52-120[»]
5FCJX-ray3.10O5/o52-120[»]
5FL8electron microscopy9.50h1-120[»]
5GAKelectron microscopy3.88j1-120[»]
5H4Pelectron microscopy3.07h1-120[»]
5I4LX-ray3.10O5/o52-120[»]
5JCSelectron microscopy9.50h1-120[»]
5JUOelectron microscopy4.00MA1-120[»]
5JUPelectron microscopy3.50MA1-120[»]
5JUSelectron microscopy4.20MA1-120[»]
5JUTelectron microscopy4.00MA1-120[»]
5JUUelectron microscopy4.00MA1-120[»]
5LYBX-ray3.25O5/o52-120[»]
5M1Jelectron microscopy3.30h52-120[»]
5MC6electron microscopy3.80BP1-120[»]
5T62electron microscopy3.30u1-120[»]
5T6Relectron microscopy4.50u1-120[»]
5TGAX-ray3.30O5/o52-120[»]
5TGMX-ray3.50O5/o52-120[»]
ProteinModelPortaliP0CX84.
SMRiP0CX84.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX84.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP0CX84.
KOiK02918.
OMAiTVINAKQ.
OrthoDBiEOG092C5O2N.

Family and domain databases

CDDicd00427. Ribosomal_L29_HIP. 1 hit.
HAMAPiMF_00374. Ribosomal_L29. 1 hit.
InterProiView protein in InterPro
IPR001854. Ribosomal_L29/L35.
IPR018254. Ribosomal_L29_CS.
PfamiView protein in Pfam
PF00831. Ribosomal_L29. 1 hit.
SUPFAMiSSF46561. SSF46561. 1 hit.
TIGRFAMsiTIGR00012. L29. 1 hit.
PROSITEiView protein in PROSITE
PS00579. RIBOSOMAL_L29. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS
60 70 80 90 100
IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALRR ALTKFEASQV
110 120
TEKQRKKQIA FPQRKYAIKA
Length:120
Mass (Da):13,910
Last modified:June 28, 2011 - v1
Checksum:iD8AC9DF157AD2AD6
GO

Mass spectrometryi

Molecular mass is 13769.991 Da from positions 2 - 120. Determined by ESI. Monoisotopic mass.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M82913 Genomic DNA. Translation: AAA34492.1.
X83276 Genomic DNA. Translation: CAA58256.1.
Z74239 Genomic DNA. Translation: CAA98768.1.
BK006938 Genomic DNA. Translation: DAA11722.1.
PIRiS30770.
RefSeqiNP_010090.1. NM_001180251.2.
NP_010145.1. NM_001180196.2.

Genome annotation databases

EnsemblFungiiYDL136W; YDL136W; YDL136W.
YDL191W; YDL191W; YDL191W.
GeneIDi851336.
851419.
KEGGisce:YDL136W.
sce:YDL191W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRL35A_YEAST
AccessioniPrimary (citable) accession number: P0CX84
Secondary accession number(s): D6VRG2, P39741, P39930
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: July 5, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names