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P0CX84

- RL35A_YEAST

UniProt

P0CX84 - RL35A_YEAST

Protein

60S ribosomal protein L35-A

Gene

RPL35A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29576-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L35-A
    Gene namesi
    Name:RPL35A
    Synonyms:SOS1
    Ordered Locus Names:YDL191W
    ORF Names:D1249
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL191w.
    SGDiS000002350. RPL35A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD
    2. preribosome, large subunit precursor Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 12011960S ribosomal protein L35-APRO_0000130552Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131Phosphoserine1 Publication
    Modified residuei50 – 501Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP0CX84.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi31854. 98 interactions.
    31925. 131 interactions.
    IntActiP0CX84. 1 interaction.
    MINTiMINT-8285624.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Ymodel-Y2-60[»]
    1S1Ielectron microscopy11.70X1-120[»]
    1VW8electron microscopy6.10i1-120[»]
    1VWUelectron microscopy6.10i1-120[»]
    2WW9electron microscopy8.60N1-120[»]
    2WWAelectron microscopy8.90N1-120[»]
    2WWBelectron microscopy6.48N1-120[»]
    3IZSelectron microscopy8.80c1-120[»]
    3J65electron microscopy8.70Z1-120[»]
    3O58X-ray4.00c1-120[»]
    3O5HX-ray4.00c1-120[»]
    3U5EX-ray3.00h1-120[»]
    3U5IX-ray3.00h1-120[»]
    4B6Aelectron microscopy8.10h1-120[»]
    4CUWelectron microscopy3.70h1-120[»]
    ProteinModelPortaliP0CX84.
    SMRiP0CX84. Positions 2-120.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0CX84.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L29P family.Curated

    Phylogenomic databases

    KOiK02918.
    OrthoDBiEOG71P2Q2.

    Family and domain databases

    Gene3Di1.10.287.310. 1 hit.
    HAMAPiMF_00374. Ribosomal_L29.
    InterProiIPR001854. Ribosomal_L29.
    IPR018254. Ribosomal_L29_CS.
    [Graphical view]
    PfamiPF00831. Ribosomal_L29. 1 hit.
    [Graphical view]
    SUPFAMiSSF46561. SSF46561. 1 hit.
    TIGRFAMsiTIGR00012. L29. 1 hit.
    PROSITEiPS00579. RIBOSOMAL_L29. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CX84-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS    50
    IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALRR ALTKFEASQV 100
    TEKQRKKQIA FPQRKYAIKA 120
    Length:120
    Mass (Da):13,910
    Last modified:June 28, 2011 - v1
    Checksum:iD8AC9DF157AD2AD6
    GO

    Mass spectrometryi

    Molecular mass is 13769.991 Da from positions 2 - 120. Determined by ESI. Monoisotopic mass.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82913 Genomic DNA. Translation: AAA34492.1.
    X83276 Genomic DNA. Translation: CAA58256.1.
    Z74239 Genomic DNA. Translation: CAA98768.1.
    BK006938 Genomic DNA. Translation: DAA11722.1.
    PIRiS30770.
    RefSeqiNP_010090.1. NM_001180251.2.
    NP_010145.1. NM_001180196.2.

    Genome annotation databases

    EnsemblFungiiYDL136W; YDL136W; YDL136W.
    YDL191W; YDL191W; YDL191W.
    GeneIDi851336.
    851419.
    KEGGisce:YDL136W.
    sce:YDL191W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82913 Genomic DNA. Translation: AAA34492.1 .
    X83276 Genomic DNA. Translation: CAA58256.1 .
    Z74239 Genomic DNA. Translation: CAA98768.1 .
    BK006938 Genomic DNA. Translation: DAA11722.1 .
    PIRi S30770.
    RefSeqi NP_010090.1. NM_001180251.2.
    NP_010145.1. NM_001180196.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5Y model - Y 2-60 [» ]
    1S1I electron microscopy 11.70 X 1-120 [» ]
    1VW8 electron microscopy 6.10 i 1-120 [» ]
    1VWU electron microscopy 6.10 i 1-120 [» ]
    2WW9 electron microscopy 8.60 N 1-120 [» ]
    2WWA electron microscopy 8.90 N 1-120 [» ]
    2WWB electron microscopy 6.48 N 1-120 [» ]
    3IZS electron microscopy 8.80 c 1-120 [» ]
    3J65 electron microscopy 8.70 Z 1-120 [» ]
    3O58 X-ray 4.00 c 1-120 [» ]
    3O5H X-ray 4.00 c 1-120 [» ]
    3U5E X-ray 3.00 h 1-120 [» ]
    3U5I X-ray 3.00 h 1-120 [» ]
    4B6A electron microscopy 8.10 h 1-120 [» ]
    4CUW electron microscopy 3.70 h 1-120 [» ]
    ProteinModelPortali P0CX84.
    SMRi P0CX84. Positions 2-120.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31854. 98 interactions.
    31925. 131 interactions.
    IntActi P0CX84. 1 interaction.
    MINTi MINT-8285624.

    Proteomic databases

    MaxQBi P0CX84.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL136W ; YDL136W ; YDL136W .
    YDL191W ; YDL191W ; YDL191W .
    GeneIDi 851336.
    851419.
    KEGGi sce:YDL136W.
    sce:YDL191W.

    Organism-specific databases

    CYGDi YDL191w.
    SGDi S000002350. RPL35A.

    Phylogenomic databases

    KOi K02918.
    OrthoDBi EOG71P2Q2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29576-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P0CX84.
    NextBioi 968405.

    Family and domain databases

    Gene3Di 1.10.287.310. 1 hit.
    HAMAPi MF_00374. Ribosomal_L29.
    InterProi IPR001854. Ribosomal_L29.
    IPR018254. Ribosomal_L29_CS.
    [Graphical view ]
    Pfami PF00831. Ribosomal_L29. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46561. SSF46561. 1 hit.
    TIGRFAMsi TIGR00012. L29. 1 hit.
    PROSITEi PS00579. RIBOSOMAL_L29. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast SIS1 protein, a DnaJ homolog, is required for the initiation of translation."
      Zhong T., Arndt K.T.
      Cell 73:1175-1186(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The sequence of 23 kb surrounding the SNF3 locus on the left arm of yeast chromosome IV reveals the location of five known genes and characterizes at least six new open reading frames including putative genes for ribosomal protein L35 and a sugar transport protein."
      Verhasselt P., Voet M., Mathys J., Volckaert G.
      Yeast 12:1065-1070(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    6. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
      Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
      Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13 AND SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 2-60, ELECTRON MICROSCOPY.
    11. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
    12. "Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome."
      Becker T., Bhushan S., Jarasch A., Armache J.P., Funes S., Jossinet F., Gumbart J., Mielke T., Berninghausen O., Schulten K., Westhof E., Gilmore R., Mandon E.C., Beckmann R.
      Science 326:1369-1373(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.60 ANGSTROMS).
    13. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.80 ANGSTROMS).
    14. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.00 ANGSTROMS).
    15. Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS).
    16. "Cryo-EM structures of Arx1 and maturation factors Rei1 and Jjj1 bound to the 60S ribosomal subunit."
      Greber B.J., Boehringer D., Montellese C., Ban N.
      Nat. Struct. Mol. Biol. 19:1228-1233(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.10 ANGSTROMS).

    Entry informationi

    Entry nameiRL35A_YEAST
    AccessioniPrimary (citable) accession number: P0CX84
    Secondary accession number(s): D6VRG2, P39741, P39930
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 2 genes for L35 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3