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Protein

60S ribosomal protein L19-B

Gene

RPL19B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. eL19 may play a role in the last stages of translation initiation, in particular sububit joining and shedding/releasing factors.1 Publication

Miscellaneous

Present with 225000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eL19 in yeast.Curated

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-28930-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L19-B1 Publication
Alternative name(s):
L23
Large ribosomal subunit protein eL191 Publication
RP15L
RP33
YL14
Gene namesi
Name:RPL19B1 Publication
Synonyms:RPL23B, YL14B
Ordered Locus Names:YBL027W
ORF Names:YBL0424
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

SGDiS000000123. RPL19B.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00004104462 – 18960S ribosomal protein L19-BAdd BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki21Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei30PhosphoserineCombined sources1
Modified residuei37PhosphoserineCombined sources1
Cross-linki53Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki60Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei91PhosphoserineCombined sources1
Cross-linki146Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki186Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP0CX83.

PTM databases

iPTMnetiP0CX83.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX83. differential.

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). eL19 lies in close proximity to the binding site for eukaryotic initiation factor eIF4G (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi32671. 272 interactors.
32789. 118 interactors.
IntActiP0CX83. 3 interactors.
MINTiMINT-519961.

Chemistry databases

BindingDBiP0CX83.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V8Telectron microscopy8.10R1-189[»]
4V8Yelectron microscopy4.30BR2-189[»]
4V8Zelectron microscopy6.60BR2-189[»]
ProteinModelPortaliP0CX83.
SMRiP0CX83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000012628.
InParanoidiP0CX83.
KOiK02885.
OrthoDBiEOG092C00Q9.

Family and domain databases

CDDicd01417. Ribosomal_L19e_E. 1 hit.
Gene3Di1.10.1200.60. 1 hit.
1.10.1650.10. 1 hit.
1.20.5.560. 1 hit.
HAMAPiMF_01475. Ribosomal_L19e. 1 hit.
InterProiView protein in InterPro
IPR000196. Ribosomal_L19/L19e.
IPR023638. Ribosomal_L19/L19e_CS.
IPR015972. Ribosomal_L19/L19e_dom1.
IPR015973. Ribosomal_L19/L19e_dom2.
IPR015974. Ribosomal_L19/L19e_dom3.
IPR033935. Ribosomal_L19_euka.
PfamiView protein in Pfam
PF01280. Ribosomal_L19e. 1 hit.
SMARTiView protein in SMART
SM01416. Ribosomal_L19e. 1 hit.
SUPFAMiSSF48140. SSF48140. 1 hit.
PROSITEiView protein in PROSITE
PS00526. RIBOSOMAL_L19E. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLRTQKRL AASVVGVGKR KVWLDPNETS EIAQANSRNA IRKLVKNGTI
60 70 80 90 100
VKKAVTVHSK SRTRAHAQSK REGRHSGYGK RKGTREARLP SQVVWIRRLR
110 120 130 140 150
VLRRLLAKYR DAGKIDKHLY HVLYKESKGN AFKHKRALVE HIIQAKADAQ
160 170 180
REKALNEEAE ARRLKNRAAR DRRAQRVAEK RDALLKEDA
Length:189
Mass (Da):21,704
Last modified:June 28, 2011 - v1
Checksum:i776C3AB3D348EE99
GO

Sequence cautioni

The sequence CAA54504 differs from that shown. Reason: Erroneous gene model prediction.Curated

Mass spectrometryi

Molecular mass is 21573.159 Da from positions 2 - 189. Determined by ESI. Average mass.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16015 Genomic DNA. Translation: AAB60318.1.
D17338 mRNA. Translation: BAA04156.1.
X77291 Genomic DNA. Translation: CAA54504.1. Sequence problems.
Z35788 Genomic DNA. Translation: CAA84846.1.
BK006936 Genomic DNA. Translation: DAA07093.1.
PIRiS44597.
RefSeqiNP_009526.1. NM_001178267.1.
NP_009641.1. NM_001180057.1.

Genome annotation databases

EnsemblFungiiYBL027W; YBL027W; YBL027W.
YBR084C-A; YBR084C-A; YBR084C-A.
GeneIDi852254.
852379.
KEGGisce:YBL027W.
sce:YBR084C-A.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRL19B_YEAST
AccessioniPrimary (citable) accession number: P0CX83
Secondary accession number(s): D6VPX3, P05735
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: July 5, 2017
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names