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Protein

60S ribosomal protein L19-A

Gene

RPL19A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29228-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L19-A
Alternative name(s):
L23
RP15L
RP33
YL14
Gene namesi
Name:RPL19A
Synonyms:RPL23A, YL14A
Ordered Locus Names:YBR084C-A
ORF Names:YBR084BC
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

SGDiS000002156. RPL19A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 18918860S ribosomal protein L19-APRO_0000131184Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Phosphoserine1 Publication
Modified residuei37 – 371Phosphoserine1 Publication
Modified residuei91 – 911Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CX82.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX82. differential.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi32671. 131 interactions.
32789. 39 interactions.
IntActiP0CX82. 1 interaction.
MINTiMINT-8285580.

Structurei

Secondary structure

1
189
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1511Combined sources
Helixi19 – 213Combined sources
Beta strandi22 – 243Combined sources
Helixi26 – 283Combined sources
Helixi29 – 335Combined sources
Helixi38 – 469Combined sources
Beta strandi49 – 513Combined sources
Helixi61 – 7111Combined sources
Turni72 – 754Combined sources
Helixi78 – 803Combined sources
Helixi85 – 884Combined sources
Helixi91 – 11020Combined sources
Turni111 – 1133Combined sources
Helixi117 – 12711Combined sources
Beta strandi129 – 1313Combined sources
Beta strandi132 – 1343Combined sources
Helixi135 – 17541Combined sources
Turni176 – 1783Combined sources
Helixi183 – 1864Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-P3-143[»]
2WW9electron microscopy8.60J1-189[»]
2WWAelectron microscopy8.90J1-189[»]
3J6Xelectron microscopy6.10591-189[»]
3J6Yelectron microscopy6.10591-189[»]
3J77electron microscopy6.20691-189[»]
3J78electron microscopy6.30691-189[»]
4U3MX-ray3.00M9/m92-189[»]
4U3NX-ray3.20M9/m92-189[»]
4U3UX-ray2.90M9/m92-189[»]
4U4NX-ray3.10M9/m92-189[»]
4U4OX-ray3.60M9/m92-189[»]
4U4QX-ray3.00M9/m92-189[»]
4U4RX-ray2.80M9/m92-189[»]
4U4UX-ray3.00M9/m92-189[»]
4U4YX-ray3.20M9/m92-189[»]
4U4ZX-ray3.10M9/m92-189[»]
4U50X-ray3.20M9/m92-189[»]
4U51X-ray3.20M9/m92-189[»]
4U52X-ray3.00M9/m92-189[»]
4U53X-ray3.30M9/m92-189[»]
4U55X-ray3.20M9/m92-189[»]
4U56X-ray3.45M9/m92-189[»]
4U6FX-ray3.10M9/m92-189[»]
4V4Belectron microscopy11.70BP2-143[»]
4V6Ielectron microscopy8.80BT1-189[»]
4V7Felectron microscopy8.70R1-189[»]
4V7RX-ray4.00BS/DS1-189[»]
4V88X-ray3.00BR/DR1-189[»]
4V91electron microscopy3.70R1-189[»]
ProteinModelPortaliP0CX82.
SMRiP0CX82. Positions 2-189.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX82.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L19e family.Curated

Phylogenomic databases

InParanoidiP0CX82.
KOiK02885.
OrthoDBiEOG7FJH7S.

Family and domain databases

Gene3Di1.10.1200.60. 1 hit.
1.10.1650.10. 1 hit.
HAMAPiMF_01475. Ribosomal_L19e.
InterProiIPR027547. Ribosomal_L19/L19e.
IPR023638. Ribosomal_L19/L19e_CS.
IPR000196. Ribosomal_L19/L19e_dom.
IPR015972. Ribosomal_L19/L19e_dom1.
IPR015974. Ribosomal_L19/L19e_dom3.
[Graphical view]
PfamiPF01280. Ribosomal_L19e. 1 hit.
[Graphical view]
SUPFAMiSSF48140. SSF48140. 1 hit.
PROSITEiPS00526. RIBOSOMAL_L19E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLRTQKRL AASVVGVGKR KVWLDPNETS EIAQANSRNA IRKLVKNGTI
60 70 80 90 100
VKKAVTVHSK SRTRAHAQSK REGRHSGYGK RKGTREARLP SQVVWIRRLR
110 120 130 140 150
VLRRLLAKYR DAGKIDKHLY HVLYKESKGN AFKHKRALVE HIIQAKADAQ
160 170 180
REKALNEEAE ARRLKNRAAR DRRAQRVAEK RDALLKEDA
Length:189
Mass (Da):21,704
Last modified:June 28, 2011 - v1
Checksum:i776C3AB3D348EE99
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541A → S in J03724 (PubMed:2836393).Curated

Mass spectrometryi

Molecular mass is 21573.159 Da from positions 2 - 189. Determined by ESI. Average mass.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36751 Genomic DNA. Translation: CAA85322.1.
D17337 mRNA. Translation: BAA04155.1.
Z35953 Genomic DNA. Translation: CAA85030.1.
Z35954 Genomic DNA. Translation: CAA85032.1.
J03724 Genomic DNA. No translation available.
BK006936 Genomic DNA. Translation: DAA07204.1.
PIRiS44597.
RefSeqiNP_009526.1. NM_001178267.1.
NP_009641.1. NM_001180057.1.

Genome annotation databases

EnsemblFungiiYBL027W; YBL027W; YBL027W.
YBR084C-A; YBR084C-A; YBR084C-A.
GeneIDi852254.
852379.
KEGGisce:YBL027W.
sce:YBR084C-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36751 Genomic DNA. Translation: CAA85322.1.
D17337 mRNA. Translation: BAA04155.1.
Z35953 Genomic DNA. Translation: CAA85030.1.
Z35954 Genomic DNA. Translation: CAA85032.1.
J03724 Genomic DNA. No translation available.
BK006936 Genomic DNA. Translation: DAA07204.1.
PIRiS44597.
RefSeqiNP_009526.1. NM_001178267.1.
NP_009641.1. NM_001180057.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-P3-143[»]
2WW9electron microscopy8.60J1-189[»]
2WWAelectron microscopy8.90J1-189[»]
3J6Xelectron microscopy6.10591-189[»]
3J6Yelectron microscopy6.10591-189[»]
3J77electron microscopy6.20691-189[»]
3J78electron microscopy6.30691-189[»]
4U3MX-ray3.00M9/m92-189[»]
4U3NX-ray3.20M9/m92-189[»]
4U3UX-ray2.90M9/m92-189[»]
4U4NX-ray3.10M9/m92-189[»]
4U4OX-ray3.60M9/m92-189[»]
4U4QX-ray3.00M9/m92-189[»]
4U4RX-ray2.80M9/m92-189[»]
4U4UX-ray3.00M9/m92-189[»]
4U4YX-ray3.20M9/m92-189[»]
4U4ZX-ray3.10M9/m92-189[»]
4U50X-ray3.20M9/m92-189[»]
4U51X-ray3.20M9/m92-189[»]
4U52X-ray3.00M9/m92-189[»]
4U53X-ray3.30M9/m92-189[»]
4U55X-ray3.20M9/m92-189[»]
4U56X-ray3.45M9/m92-189[»]
4U6FX-ray3.10M9/m92-189[»]
4V4Belectron microscopy11.70BP2-143[»]
4V6Ielectron microscopy8.80BT1-189[»]
4V7Felectron microscopy8.70R1-189[»]
4V7RX-ray4.00BS/DS1-189[»]
4V88X-ray3.00BR/DR1-189[»]
4V91electron microscopy3.70R1-189[»]
ProteinModelPortaliP0CX82.
SMRiP0CX82. Positions 2-189.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32671. 131 interactions.
32789. 39 interactions.
IntActiP0CX82. 1 interaction.
MINTiMINT-8285580.

Chemistry

BindingDBiP0CX82.
ChEMBLiCHEMBL1741172.

Proteomic databases

MaxQBiP0CX82.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL027W; YBL027W; YBL027W.
YBR084C-A; YBR084C-A; YBR084C-A.
GeneIDi852254.
852379.
KEGGisce:YBL027W.
sce:YBR084C-A.

Organism-specific databases

SGDiS000002156. RPL19A.

Phylogenomic databases

InParanoidiP0CX82.
KOiK02885.
OrthoDBiEOG7FJH7S.

Enzyme and pathway databases

BioCyciYEAST:G3O-29228-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

EvolutionaryTraceiP0CX82.
NextBioi970832.

Gene expression databases

ExpressionAtlasiP0CX82. differential.

Family and domain databases

Gene3Di1.10.1200.60. 1 hit.
1.10.1650.10. 1 hit.
HAMAPiMF_01475. Ribosomal_L19e.
InterProiIPR027547. Ribosomal_L19/L19e.
IPR023638. Ribosomal_L19/L19e_CS.
IPR000196. Ribosomal_L19/L19e_dom.
IPR015972. Ribosomal_L19/L19e_dom1.
IPR015974. Ribosomal_L19/L19e_dom3.
[Graphical view]
PfamiPF01280. Ribosomal_L19e. 1 hit.
[Graphical view]
SUPFAMiSSF48140. SSF48140. 1 hit.
PROSITEiPS00526. RIBOSOMAL_L19E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and characterization of the Saccharomyces cerevisiae RPL19A gene encoding a homolog of the mammalian ribosomal protein L19."
    Song J.M., Cheung E., Rabinowitz J.C.
    Yeast 11:383-389(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Suzuki K., Tomiyoshi A., Otaka E.
    Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe."
    Otaka E., Higo K., Itoh T.
    Mol. Gen. Genet. 191:519-524(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-31.
  6. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
    Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
    J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21.
  7. "Isolation and characterization of the Saccharomyces cerevisiae MIS1 gene encoding mitochondrial C1-tetrahydrofolate synthase."
    Shannon K.W., Rabinowitz J.C.
    J. Biol. Chem. 263:7717-7725(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-189.
  8. "Identification of protein coding regions by database similarity search."
    Gish W., States D.J.
    Nat. Genet. 3:266-272(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  9. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  10. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
    Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
    Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
  11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30; SER-37 AND SER-91, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 3-143, ELECTRON MICROSCOPY.
  18. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
  19. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  20. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL19A_YEAST
AccessioniPrimary (citable) accession number: P0CX82
Secondary accession number(s): D6VPX3, P05735
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: March 4, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 97700 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for L19 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.