P0CX82 (RL19A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L19-A

Alternative name(s):
L23
RP15L
RP33
YL14

Gene names

Name:	RPL19A
Synonyms:	RPL23A, YL14A
Ordered Locus Names:	YBR084C-A
ORF Names:	YBR084BC

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.9
Subcellular location	Cytoplasm Ref.11.
Miscellaneous	Present with 97700 molecules/cell in log phase SD medium. There are 2 genes for L19 in yeast.
Sequence similarities	Belongs to the ribosomal protein L19e family.
Mass spectrometry	Molecular mass is 21573.159 Da from positions 2 - 189. Determined by ESI. Average mass. Ref.10

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.9. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.9. Source: SGD
Molecular_function	structural constituent of ribosome Traceable author statement Ref.9. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5 Ref.6
	Chain	2 – 189	188	60S ribosomal protein L19-A		PRO_0000131184
Amino acid modifications
	Modified residue	29	1	Phosphothreonine Ref.14
	Modified residue	30	1	Phosphoserine Ref.13 Ref.14
	Modified residue	91	1	Phosphoserine Ref.13
Experimental info
	Sequence conflict	54	1	A → S in J03724. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P0CX82 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 776C3AB3D348EE99	FASTA	189	21,704
	10 20 30 40 50 60 MANLRTQKRL AASVVGVGKR KVWLDPNETS EIAQANSRNA IRKLVKNGTI VKKAVTVHSK 70 80 90 100 110 120 SRTRAHAQSK REGRHSGYGK RKGTREARLP SQVVWIRRLR VLRRLLAKYR DAGKIDKHLY 130 140 150 160 170 180 HVLYKESKGN AFKHKRALVE HIIQAKADAQ REKALNEEAE ARRLKNRAAR DRRAQRVAEK RDALLKEDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and characterization of the Saccharomyces cerevisiae RPL19A gene encoding a homolog of the mammalian ribosomal protein L19." Song J.M., Cheung E., Rabinowitz J.C. Yeast 11:383-389(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Suzuki K., Tomiyoshi A., Otaka E. Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe." Otaka E., Higo K., Itoh T. Mol. Gen. Genet. 191:519-524(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-31.
[6]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21.
[7]	"Isolation and characterization of the Saccharomyces cerevisiae MIS1 gene encoding mitochondrial C1-tetrahydrofolate synthase." Shannon K.W., Rabinowitz J.C. J. Biol. Chem. 263:7717-7725(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-189.
[8]	"Identification of protein coding regions by database similarity search." Gish W., States D.J. Nat. Genet. 3:266-272(1993) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[9]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[10]	"Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR." Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D. Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.
[11]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[12]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30 AND SER-91, MASS SPECTROMETRY. Strain: YAL6B.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29 AND SER-30, MASS SPECTROMETRY.
[15]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 3-143, ELECTRON MICROSCOPY.
[16]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z36751 Genomic DNA. Translation: CAA85322.1.
D17337 mRNA. Translation: BAA04155.1.
Z35953 Genomic DNA. Translation: CAA85030.1.
Z35954 Genomic DNA. Translation: CAA85032.1.
J03724 Genomic DNA. No translation available.
BK006936 Genomic DNA. Translation: DAA07204.1.

PIR

S44597.

RefSeq

NP_009526.1. NM_001178267.1.
NP_009641.1. NM_001180057.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	P	3-143	[»]
1S1I	electron microscopy	11.70	P	2-143	[»]
2WW9	electron microscopy	8.60	J	1-189	[»]
2WWA	electron microscopy	8.90	J	1-189	[»]
3IZS	electron microscopy	-	T	1-189	[»]
3O58	X-ray	4.00	S	1-189	[»]
3O5H	X-ray	4.00	S	1-189	[»]
3U5E	X-ray	3.00	R	1-189	[»]
3U5I	X-ray	3.00	R	1-189	[»]

ProteinModelPortal

P0CX82.

SMR

P0CX82. Positions 2-189.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBL027W; YBL027W; YBL027W.
YBR084C-A; YBR084C-A; YBR084C-A.

GeneID

852254.
852379.

KEGG

sce:YBL027W.
sce:YBR084C-A.

Organism-specific databases

SGD

S000002156. RPL19A.

Phylogenomic databases

KO

K02885.

Gene expression databases

ArrayExpress

P0CX82.

GermOnline

YBR084C-A. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.1200.60. 1 hit.
1.10.1650.10. 1 hit.

InterPro

IPR000196. Ribosomal_L19/L19e.
IPR023638. Ribosomal_L19/L19e_CS.
IPR015972. Ribosomal_L19/L19e_dom1.
IPR015974. Ribosomal_L19/L19e_dom3.
[Graphical view]

Pfam

PF01280. Ribosomal_L19e. 1 hit.
[Graphical view]

SUPFAM

SSF48140. Ribosomal_L19/L19e. 1 hit.

PROSITE

PS00526. RIBOSOMAL_L19E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P0CX82.

ChEMBL

CHEMBL1741172.

EvolutionaryTrace

P0CX82.

NextBio

970832.

Entry information

Entry name

RL19A_YEAST

Accession

Primary (citable) accession number: P0CX82
Secondary accession number(s): D6VPX3, P05735

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program