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P0CX69 (YM14A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transposon Ty1-MR2 Gag polyprotein
Alternative name(s):
Gag-p49
Transposon Ty1 protein A
Short name=TY1A
Short name=TYA
p58

Cleaved into the following 2 chains:

  1. Capsid protein
    Short name=CA
    Alternative name(s):
    Gag-p45
    p54
  2. Gag-p4
Gene names
Name:TY1A-MR2
Synonyms:YMRCTy1-4 GAG
Ordered Locus Names:YMR051C
ORF Names:YM9796.04C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the retrotransposons dimeric RNA genome. The particles are assembled from trimer-clustered units and there are holes in the capsid shells that allow for the diffusion of macromolecules. CA has also nucleocapsid-like chaperone activity, promoting primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty1 RNA and initiation of reverse transcription By similarity.

Subunit structure

Homotrimer By similarity.

Subcellular location

Cytoplasm By similarity.

Induction

Ty1-MR2 is a weakly expressed element. Induced under amino acid starvation conditions by GCN4. Ref.4

Domain

The C-terminal RNA-binding region of CA is sufficient for all its nucleocapsid-like chaperone activities By similarity.

Miscellaneous

Retrotransposons are mobile genetic entities that are able to replicate via an RNA intermediate and a reverse transcription step. In contrast to retroviruses, retrotransposons are non-infectious, lack an envelope and remain intracellular. Ty1 retrotransposons belong to the copia elements (pseudoviridae).

Ontologies

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Note: The Gag-Pol polyprotein is generated by a +1 ribosomal frameshift. The ratio of Gag:Gag-Pol varies between 20:1 and 5:1 (By similarity).
Isoform Transposon Ty1-MR2 Gag polyprotein (identifier: P0CX69-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by conventional translation.
Isoform Transposon Ty1-MR2 Gag-Pol polyprotein (identifier: Q04670-1)

The sequence of this isoform can be found in the external entry Q04670.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by +1 ribosomal frameshifting between codon Leu-435 and Gly-436 of the YMR051C ORF.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440Transposon Ty1-MR2 Gag polyprotein
PRO_0000409788
Chain1 – 401401Capsid protein By similarity
PRO_0000409789
Peptide402 – 44039Gag-p4 By similarity
PRO_0000409790

Regions

Region299 – 401103RNA-binding By similarity
Compositional bias64 – 14683Pro-rich

Sites

Site401 – 4022Cleavage; by Ty1 protease By similarity

Amino acid modifications

Modified residue4161Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform Transposon Ty1-MR2 Gag polyprotein [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 7418E94ED2778F8F

FASTA44049,129
        10         20         30         40         50         60 
MESQQLSNYP HISHGSACAS VTSKEVHTNQ DPLDVSASKI QEYDKASTKA NSQQTTTPAS 

        70         80         90        100        110        120 
SAVPENPHHA SPQPASVPPP QNGPYPQQCM MTQNQANPSG WSFYGHPSMI PYTPYQMSPM 

       130        140        150        160        170        180 
YFPPGPQSQF PQYPSSVGTP LSTPSPESGN TFTDSSSADS DMTSTKKYVR PPPMLTSPND 

       190        200        210        220        230        240 
FPNWVKTYIK FLQNSNLGGI IPTVNGKPVR QITDDELTFL YNTFQIFAPS QFLPTWVKDI 

       250        260        270        280        290        300 
LSVDYTDIMK ILSKSIEKMQ SDTQEANDIV TLANLQYNGS TPADAFETKV TNIIDRLNNN 

       310        320        330        340        350        360 
GIHINNKVAC QLIMRGLSGE YKFLRYTRHR HLNMTVAELF LDIHAIYEEQ QGSRNSKPNY 

       370        380        390        400        410        420 
RRNPSDEKND SRSYTNTTKP KVIARNPQKT NNSKSKTARA HNVSTSNNSP STDNDSISKS 

       430        440 
TTEPIQLNNK HDLHLRPETY 

« Hide

Isoform Transposon Ty1-MR2 Gag-Pol polyprotein [UniParc].

See Q04670.

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence."
Kim J.M., Vanguri S., Boeke J.D., Gabriel A., Voytas D.F.
Genome Res. 8:464-478(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
[4]"Differential effects of chromatin and Gcn4 on the 50-fold range of expression among individual yeast Ty1 retrotransposons."
Morillon A., Benard L., Springer M., Lesage P.
Mol. Cell. Biol. 22:2078-2088(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[5]"Happy together: the life and times of Ty retrotransposons and their hosts."
Lesage P., Todeschini A.L.
Cytogenet. Genome Res. 110:70-90(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49703 Genomic DNA. Translation: CAA89761.1.
BK006946 Genomic DNA. Translation: DAA09950.1.
PIRS53588.
RefSeqNP_013766.1. NM_001182548.1.
NP_058151.1. NM_001184424.1.
NP_058156.1. NM_001184429.1.
NP_058166.1. NM_001184399.1.

3D structure databases

ProteinModelPortalP0CX69.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32371. 2 interactions.
33412. 5 interactions.
35226. 3 interactions.
36913. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR316W-A; YDR316W-A; YDR316W-A.
YER159C-A; YER159C-A; YER159C-A.
YGR161C-C; YGR161C-C; YGR161C-C.
YMR051C; YMR051C; YMR051C.
GeneID851913.
853069.
855071.
856907.
KEGGsce:YDR316W-A.
sce:YER159C-A.
sce:YGR161C-C.
sce:YMR051C.

Organism-specific databases

CYGDYMR051c.
SGDS000004654. YMR051C.

Phylogenomic databases

GeneTreeENSGT00750000117913.
OrthoDBEOG7TJ3S3.

Family and domain databases

InterProIPR015820. Retrotransposon_Ty1A_N.
[Graphical view]
PfamPF01021. TYA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio969941.

Entry information

Entry nameYM14A_YEAST
AccessionPrimary (citable) accession number: P0CX69
Secondary accession number(s): D3DM68, Q12231
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 16, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD