Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0CX66

- YE12A_YEAST

UniProt

P0CX66 - YE12A_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Transposon Ty1-ER2 Gag polyprotein

Gene

TY1A-ER2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the retrotransposons dimeric RNA genome. The particles are assembled from trimer-clustered units and there are holes in the capsid shells that allow for the diffusion of macromolecules. CA has also nucleocapsid-like chaperone activity, promoting primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty1 RNA and initiation of reverse transcription (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei401 – 4022Cleavage; by Ty1 proteaseBy similarity

GO - Molecular functioni

  1. RNA binding Source: SGD

GO - Biological processi

  1. transposition, RNA-mediated Source: SGD
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transposon Ty1-ER2 Gag polyprotein
Alternative name(s):
Gag-p49
Transposon Ty1 protein A
Short name:
TY1A
Short name:
TYA
p58
Cleaved into the following 2 chains:
Capsid protein
Short name:
CA
Alternative name(s):
Gag-p45
p54
Gene namesi
Name:TY1A-ER2
Synonyms:YERCTy1-2 GAG
Ordered Locus Names:YER159C-A
ORF Names:YER160C-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER159c-a.
SGDiS000007403. YER159C-A.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. retrotransposon nucleocapsid Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Transposon Ty1-ER2 Gag polyproteinPRO_0000409779Add
BLAST
Chaini1 – 401401Capsid proteinBy similarityPRO_0000409780Add
BLAST
Peptidei402 – 44039Gag-p4By similarityPRO_0000409781Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei416 – 4161PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Ty1-ER2 is a weakly expressed element. Induced under amino acid starvation conditions by GCN4.1 Publication

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

BioGridi32371. 3 interactions.
33412. 6 interactions.
35226. 5 interactions.
36913. 3 interactions.
IntActiP0CX66. 1 interaction.
MINTiMINT-8285702.

Structurei

3D structure databases

ProteinModelPortaliP0CX66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni299 – 401103RNA-bindingBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi64 – 14683Pro-richAdd
BLAST

Domaini

The C-terminal RNA-binding region of CA is sufficient for all its nucleocapsid-like chaperone activities.By similarity

Phylogenomic databases

InParanoidiP0CX66.
OrthoDBiEOG7TJ3S3.

Family and domain databases

InterProiIPR015820. Retrotransposon_Ty1A_N.
[Graphical view]
PfamiPF01021. TYA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Note: The Gag-Pol polyprotein is generated by a +1 ribosomal frameshift. The ratio of Gag:Gag-Pol varies between 20:1 and 5:1 (By similarity).By similarity

Isoform Transposon Ty1-ER2 Gag polyprotein (identifier: P0CX66-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESQQLSNYP HISHGSACAS VTSKEVHTNQ DPLDVSASKI QEYDKASTKA
60 70 80 90 100
NSQQTTTPAS SAVPENPHHA SPQPASVPPP QNGPYPQQCM MTQNQANPSG
110 120 130 140 150
WSFYGHPSMI PYTPYQMSPM YFPPGPQSQF PQYPSSVGTP LSTPSPESGN
160 170 180 190 200
TFTDSSSADS DMTSTKKYVR PPPMLTSPND FPNWVKTYIK FLQNSNLGGI
210 220 230 240 250
IPTVNGKPVR QITDDELTFL YNTFQIFAPS QFLPTWVKDI LSVDYTDIMK
260 270 280 290 300
ILSKSIEKMQ SDTQEANDIV TLANLQYNGS TPADAFETKV TNIIDRLNNN
310 320 330 340 350
GIHINNKVAC QLIMRGLSGE YKFLRYTRHR HLNMTVAELF LDIHAIYEEQ
360 370 380 390 400
QGSRNSKPNY RRNPSDEKND SRSYTNTTKP KVIARNPQKT NNSKSKTARA
410 420 430 440
HNVSTSNNSP STDNDSISKS TTEPIQLNNK HDLHLRPETY

Note: Produced by conventional translation.

Length:440
Mass (Da):49,129
Last modified:June 28, 2011 - v1
Checksum:i7418E94ED2778F8F
GO
Isoform Transposon Ty1-ER2 Gag-Pol polyprotein (identifier: Q03619-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q03619.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by +1 ribosomal frameshifting between codon Leu-435 and Gly-436 of the YER159C-A ORF.

Length:1,755
Mass (Da):198,570
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18917 Genomic DNA. No translation available.
BK006939 Genomic DNA. Translation: DAA07822.1.
PIRiS53588.
RefSeqiNP_013766.1. NM_001182548.1. [P0CX66-1]
NP_058151.1. NM_001184424.1. [P0CX66-1]
NP_058156.1. NM_001184429.1. [P0CX66-1]
NP_058166.1. NM_001184399.1. [P0CX66-1]

Genome annotation databases

EnsemblFungiiYDR316W-A; YDR316W-A; YDR316W-A.
YER159C-A; YER159C-A; YER159C-A.
YGR161C-C; YGR161C-C; YGR161C-C.
YMR051C; YMR051C; YMR051C.
GeneIDi851913.
853069.
855071.
856907.
KEGGisce:YDR316W-A.
sce:YER159C-A.
sce:YGR161C-C.
sce:YMR051C.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18917 Genomic DNA. No translation available.
BK006939 Genomic DNA. Translation: DAA07822.1 .
PIRi S53588.
RefSeqi NP_013766.1. NM_001182548.1. [P0CX66-1 ]
NP_058151.1. NM_001184424.1. [P0CX66-1 ]
NP_058156.1. NM_001184429.1. [P0CX66-1 ]
NP_058166.1. NM_001184399.1. [P0CX66-1 ]

3D structure databases

ProteinModelPortali P0CX66.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32371. 3 interactions.
33412. 6 interactions.
35226. 5 interactions.
36913. 3 interactions.
IntActi P0CX66. 1 interaction.
MINTi MINT-8285702.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR316W-A ; YDR316W-A ; YDR316W-A .
YER159C-A ; YER159C-A ; YER159C-A .
YGR161C-C ; YGR161C-C ; YGR161C-C .
YMR051C ; YMR051C ; YMR051C .
GeneIDi 851913.
853069.
855071.
856907.
KEGGi sce:YDR316W-A.
sce:YER159C-A.
sce:YGR161C-C.
sce:YMR051C.

Organism-specific databases

CYGDi YER159c-a.
SGDi S000007403. YER159C-A.

Phylogenomic databases

InParanoidi P0CX66.
OrthoDBi EOG7TJ3S3.

Miscellaneous databases

NextBioi 969941.

Family and domain databases

InterProi IPR015820. Retrotransposon_Ty1A_N.
[Graphical view ]
Pfami PF01021. TYA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence."
    Kim J.M., Vanguri S., Boeke J.D., Gabriel A., Voytas D.F.
    Genome Res. 8:464-478(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  4. "Differential effects of chromatin and Gcn4 on the 50-fold range of expression among individual yeast Ty1 retrotransposons."
    Morillon A., Benard L., Springer M., Lesage P.
    Mol. Cell. Biol. 22:2078-2088(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Happy together: the life and times of Ty retrotransposons and their hosts."
    Lesage P., Todeschini A.L.
    Cytogenet. Genome Res. 110:70-90(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiYE12A_YEAST
AccessioniPrimary (citable) accession number: P0CX66
Secondary accession number(s): D3DM68, Q12231
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: October 29, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Retrotransposons are mobile genetic entities that are able to replicate via an RNA intermediate and a reverse transcription step. In contrast to retroviruses, retrotransposons are non-infectious, lack an envelope and remain intracellular. Ty1 retrotransposons belong to the copia elements (pseudoviridae).

Keywords - Technical termi

Complete proteome, Reference proteome, Transposable element

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3