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Protein

40S ribosomal protein S18-A

Gene

RPS18A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA.By similarity

GO - Molecular functioni

  • rRNA binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • rRNA export from nucleus Source: SGD
  • translation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29981-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S18-A
Gene namesi
Name:RPS18A
Ordered Locus Names:YDR450W
ORF Names:D9461.35
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

SGDiS000002858. RPS18A.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • cytosolic small ribosomal subunit Source: SGD
  • small ribosomal subunit Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001322272 – 14640S ribosomal protein S18-AAdd BLAST145

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources2 Publications1
Cross-linki36Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei48N6-methyllysine; by RKM11 Publication1
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki80Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki96Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.2 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

MaxQBiP0CX55.
PRIDEiP0CX55.
TopDownProteomicsiP0CX55.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX55. baseline.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi32505. 52 interactors.
35143. 63 interactors.
IntActiP0CX55. 2 interactors.
MINTiMINT-572872.

Structurei

Secondary structure

1146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 17Combined sources5
Beta strandi20 – 22Combined sources3
Beta strandi24 – 27Combined sources4
Helixi28 – 31Combined sources4
Helixi32 – 34Combined sources3
Helixi40 – 49Combined sources10
Turni58 – 60Combined sources3
Helixi63 – 73Combined sources11
Turni76 – 80Combined sources5
Helixi83 – 85Combined sources3
Beta strandi86 – 88Combined sources3
Beta strandi92 – 94Combined sources3
Helixi103 – 119Combined sources17
Helixi122 – 128Combined sources7
Beta strandi133 – 135Combined sources3
Beta strandi139 – 141Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-M15-145[»]
3J6Xelectron microscopy6.10181-146[»]
3J6Yelectron microscopy6.10181-146[»]
3J77electron microscopy6.20181-146[»]
3J78electron microscopy6.30181-146[»]
3V88X-ray3.00S1-146[»]
4U3MX-ray3.00C8/c82-146[»]
4U3NX-ray3.20C8/c82-146[»]
4U3UX-ray2.90C8/c82-146[»]
4U4NX-ray3.10C8/c82-146[»]
4U4OX-ray3.60C8/c82-146[»]
4U4QX-ray3.00C8/c82-146[»]
4U4RX-ray2.80C8/c82-146[»]
4U4UX-ray3.00C8/c82-146[»]
4U4YX-ray3.20C8/c82-146[»]
4U4ZX-ray3.10C8/c82-146[»]
4U50X-ray3.20C8/c82-146[»]
4U51X-ray3.20C8/c82-146[»]
4U52X-ray3.00C8/c82-146[»]
4U53X-ray3.30C8/c82-146[»]
4U55X-ray3.20C8/c82-146[»]
4U56X-ray3.45C8/c82-146[»]
4U6FX-ray3.10C8/c82-146[»]
4V4Belectron microscopy11.70AM15-146[»]
4V6Ielectron microscopy8.80AM1-146[»]
4V7RX-ray4.00AL/CL1-146[»]
4V88X-ray3.00AS/CS1-146[»]
4V8Yelectron microscopy4.30AS1-146[»]
4V8Zelectron microscopy6.60AS1-146[»]
4V92electron microscopy3.70S2-141[»]
5DATX-ray3.15C8/c82-146[»]
5DC3X-ray3.25C8/c82-146[»]
5FCIX-ray3.40C8/c82-146[»]
5FCJX-ray3.10C8/c82-146[»]
5I4LX-ray3.10C8/c82-146[»]
5JUOelectron microscopy4.00PB1-146[»]
5JUPelectron microscopy3.50PB1-146[»]
5JUSelectron microscopy4.20PB1-146[»]
5JUTelectron microscopy4.00PB1-146[»]
5JUUelectron microscopy4.00PB1-146[»]
ProteinModelPortaliP0CX55.
SMRiP0CX55.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX55.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S13P family.Curated

Phylogenomic databases

InParanoidiP0CX55.
KOiK02964.
OrthoDBiEOG092C5518.

Family and domain databases

Gene3Di4.10.910.10. 1 hit.
HAMAPiMF_01315. Ribosomal_S13_S18. 1 hit.
InterProiIPR027437. 30s_Rbsml_prot_S13_C.
IPR001892. Ribosomal_S13.
IPR010979. Ribosomal_S13-like_H2TH.
IPR018269. Ribosomal_S13_CS.
[Graphical view]
PfamiPF00416. Ribosomal_S13. 1 hit.
[Graphical view]
PIRSFiPIRSF002134. Ribosomal_S13. 1 hit.
SUPFAMiSSF46946. SSF46946. 1 hit.
PROSITEiPS00646. RIBOSOMAL_S13_1. 1 hit.
PS50159. RIBOSOMAL_S13_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLVVQEQGS FQHILRLLNT NVDGNIKIVY ALTTIKGVGR RYSNLVCKKA
60 70 80 90 100
DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQN DITDGKDYHT
110 120 130 140
LANNVESKLR DDLERLKKIR AHRGIRHFWG LRVRGQHTKT TGRRRA
Length:146
Mass (Da):17,038
Last modified:June 28, 2011 - v1
Checksum:i1F50BF9ADD20CA19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA. Translation: AAB64891.1.
BK006938 Genomic DNA. Translation: DAA12285.1.
PIRiS50886.
RefSeqiNP_010738.1. NM_001180758.1.
NP_013686.1. NM_001182384.1.

Genome annotation databases

EnsemblFungiiYDR450W; YDR450W; YDR450W.
YML026C; YML026C; YML026C.
GeneIDi852061.
854982.
KEGGisce:YDR450W.
sce:YML026C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA. Translation: AAB64891.1.
BK006938 Genomic DNA. Translation: DAA12285.1.
PIRiS50886.
RefSeqiNP_010738.1. NM_001180758.1.
NP_013686.1. NM_001182384.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-M15-145[»]
3J6Xelectron microscopy6.10181-146[»]
3J6Yelectron microscopy6.10181-146[»]
3J77electron microscopy6.20181-146[»]
3J78electron microscopy6.30181-146[»]
3V88X-ray3.00S1-146[»]
4U3MX-ray3.00C8/c82-146[»]
4U3NX-ray3.20C8/c82-146[»]
4U3UX-ray2.90C8/c82-146[»]
4U4NX-ray3.10C8/c82-146[»]
4U4OX-ray3.60C8/c82-146[»]
4U4QX-ray3.00C8/c82-146[»]
4U4RX-ray2.80C8/c82-146[»]
4U4UX-ray3.00C8/c82-146[»]
4U4YX-ray3.20C8/c82-146[»]
4U4ZX-ray3.10C8/c82-146[»]
4U50X-ray3.20C8/c82-146[»]
4U51X-ray3.20C8/c82-146[»]
4U52X-ray3.00C8/c82-146[»]
4U53X-ray3.30C8/c82-146[»]
4U55X-ray3.20C8/c82-146[»]
4U56X-ray3.45C8/c82-146[»]
4U6FX-ray3.10C8/c82-146[»]
4V4Belectron microscopy11.70AM15-146[»]
4V6Ielectron microscopy8.80AM1-146[»]
4V7RX-ray4.00AL/CL1-146[»]
4V88X-ray3.00AS/CS1-146[»]
4V8Yelectron microscopy4.30AS1-146[»]
4V8Zelectron microscopy6.60AS1-146[»]
4V92electron microscopy3.70S2-141[»]
5DATX-ray3.15C8/c82-146[»]
5DC3X-ray3.25C8/c82-146[»]
5FCIX-ray3.40C8/c82-146[»]
5FCJX-ray3.10C8/c82-146[»]
5I4LX-ray3.10C8/c82-146[»]
5JUOelectron microscopy4.00PB1-146[»]
5JUPelectron microscopy3.50PB1-146[»]
5JUSelectron microscopy4.20PB1-146[»]
5JUTelectron microscopy4.00PB1-146[»]
5JUUelectron microscopy4.00PB1-146[»]
ProteinModelPortaliP0CX55.
SMRiP0CX55.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32505. 52 interactors.
35143. 63 interactors.
IntActiP0CX55. 2 interactors.
MINTiMINT-572872.

Proteomic databases

MaxQBiP0CX55.
PRIDEiP0CX55.
TopDownProteomicsiP0CX55.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR450W; YDR450W; YDR450W.
YML026C; YML026C; YML026C.
GeneIDi852061.
854982.
KEGGisce:YDR450W.
sce:YML026C.

Organism-specific databases

SGDiS000002858. RPS18A.

Phylogenomic databases

InParanoidiP0CX55.
KOiK02964.
OrthoDBiEOG092C5518.

Enzyme and pathway databases

BioCyciYEAST:G3O-29981-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiP0CX55.
PROiP0CX55.

Gene expression databases

ExpressionAtlasiP0CX55. baseline.

Family and domain databases

Gene3Di4.10.910.10. 1 hit.
HAMAPiMF_01315. Ribosomal_S13_S18. 1 hit.
InterProiIPR027437. 30s_Rbsml_prot_S13_C.
IPR001892. Ribosomal_S13.
IPR010979. Ribosomal_S13-like_H2TH.
IPR018269. Ribosomal_S13_CS.
[Graphical view]
PfamiPF00416. Ribosomal_S13. 1 hit.
[Graphical view]
PIRSFiPIRSF002134. Ribosomal_S13. 1 hit.
SUPFAMiSSF46946. SSF46946. 1 hit.
PROSITEiPS00646. RIBOSOMAL_S13_1. 1 hit.
PS50159. RIBOSOMAL_S13_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRS18A_YEAST
AccessioniPrimary (citable) accession number: P0CX55
Secondary accession number(s): D6VT75, P35271
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 30, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 48100 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S18 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.