40S ribosomal protein S18-A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P0CX55 (RS18A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 14. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
40S ribosomal protein S18-A

Gene names

Name:	RPS18A
Ordered Locus Names:	YDR450W
ORF Names:	D9461.35

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA By similarity. HAMAP-Rule MF_01315
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm Ref.6.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA. HAMAP-Rule MF_01315
Miscellaneous	Present with 48100 molecules/cell in log phase SD medium. HAMAP-Rule MF_01315 There are 2 genes for S18 in yeast. HAMAP-Rule MF_01315
Sequence similarities	Belongs to the ribosomal protein S13P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Non-traceable author statement Ref.4. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype PubMed 14627813. Source: SGD rRNA export from nucleus Inferred from genetic interaction PubMed 16246728. Source: SGD
Cellular_component	cytosolic small ribosomal subunit Inferred from Biological aspect of Ancestor. Source: RefGenome mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD
Molecular_function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Non-traceable author statement Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.3 Ref.5

Chain

2 – 146

145

40S ribosomal protein S18-A HAMAP-Rule MF_01315

PRO_0000132227

Amino acid modifications

Modified residue

N-acetylserine Ref.3 Ref.5

Secondary structure

146

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0CX55 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 1F50BF9ADD20CA19
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FASTA

146

17,038

        10         20         30         40         50         60 
MSLVVQEQGS FQHILRLLNT NVDGNIKIVY ALTTIKGVGR RYSNLVCKKA DVDLHKRAGE 

        70         80         90        100        110        120 
LTQEELERIV QIMQNPTHYK IPAWFLNRQN DITDGKDYHT LANNVESKLR DDLERLKKIR 

       130        140 
AHRGIRHFWG LRVRGQHTKT TGRRRA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	Bienvenut W.V., Peters C. Submitted (MAY-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-36; 58-68 AND 81-88, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 15-145, ELECTRON MICROSCOPY.
[9]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 15-146, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U33007 Genomic DNA. Translation: AAB64891.1.
BK006938 Genomic DNA. Translation: DAA12285.1.

PIR

S50886.

RefSeq

NP_010738.1. NM_001180758.1.
NP_013686.1. NM_001182384.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5X	model	-	M	15-145	[»]
1S1H	electron microscopy	11.70	M	15-146	[»]
3IZB	electron microscopy	-	M	1-146	[»]
3O2Z	X-ray	4.00	L	1-146	[»]
3O30	X-ray	4.00	L	1-146	[»]
3U5C	X-ray	3.00	S	1-146	[»]
3U5G	X-ray	3.00	S	1-146	[»]

ProteinModelPortal

P0CX55.

SMR

P0CX55. Positions 2-146.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YDR450W; YDR450W; YDR450W.
YML026C; YML026C; YML026C.

GeneID

852061.
854982.

KEGG

sce:YDR450W.
sce:YML026C.

Organism-specific databases

SGD

S000002858. RPS18A.

Phylogenomic databases

K02964.

Gene expression databases

ArrayExpress

P0CX55.

GermOnline

YDR450W. Saccharomyces cerevisiae.

Family and domain databases

HAMAP

MF_01315. Ribosomal_S13/S18.

InterPro

IPR001892. Ribosomal_S13.
IPR010979. Ribosomal_S13-like_H2TH.
IPR018269. Ribosomal_S13_CS.
[Graphical view]

PANTHER

PTHR10871. PTHR10871. 1 hit.

Pfam

PF00416. Ribosomal_S13. 1 hit.
[Graphical view]

PIRSF

PIRSF002134. Ribosomal_S13. 1 hit.

SUPFAM

SSF46946. Ribosomal_H2TH. 1 hit.

PROSITE

PS00646. RIBOSOMAL_S13_1. 1 hit.
PS50159. RIBOSOMAL_S13_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0CX55.

NextBio

970337.

Entry information

Entry name

RS18A_YEAST

Accession

Primary (citable) accession number: P0CX55
Secondary accession number(s): D6VT75, P35271

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program