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Protein

40S ribosomal protein S18-A

Gene

RPS18A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 48100 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for uS13 in yeast.Curated

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • rRNA export from nucleus Source: SGD
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29981-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-SCE-72702 Ribosomal scanning and start codon recognition
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S18-A1 Publication
Alternative name(s):
Small ribosomal subunit protein uS13-A1 Publication
Gene namesi
Name:RPS18A1 Publication
Ordered Locus Names:YDR450W
ORF Names:D9461.35
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

SGDiS000002858 RPS18A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001322272 – 14640S ribosomal protein S18-AAdd BLAST145

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources2 Publications1
Cross-linki36Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei48N6-methyllysine; by RKM11 Publication1
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki80Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki96Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.2 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

MaxQBiP0CX55
PaxDbiP0CX55
PRIDEiP0CX55
TopDownProteomicsiP0CX55

PTM databases

CarbonylDBiP0CX55
iPTMnetiP0CX55

Expressioni

Gene expression databases

ExpressionAtlasiP0CX55 differential

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi32505, 148 interactors
35143, 184 interactors
IntActiP0CX55, 2 interactors
MINTiP0CX55
STRINGi4932.YML026C

Structurei

Secondary structure

1146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 17Combined sources5
Beta strandi20 – 22Combined sources3
Beta strandi24 – 27Combined sources4
Helixi28 – 31Combined sources4
Helixi32 – 34Combined sources3
Helixi40 – 49Combined sources10
Turni58 – 60Combined sources3
Helixi63 – 73Combined sources11
Turni76 – 80Combined sources5
Helixi83 – 85Combined sources3
Beta strandi86 – 88Combined sources3
Beta strandi92 – 94Combined sources3
Helixi103 – 119Combined sources17
Helixi122 – 128Combined sources7
Beta strandi133 – 135Combined sources3
Beta strandi139 – 141Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-M15-145[»]
3J6Xelectron microscopy6.10181-146[»]
3J6Yelectron microscopy6.10181-146[»]
3J77electron microscopy6.20181-146[»]
3J78electron microscopy6.30181-146[»]
4U3MX-ray3.00C8/c82-146[»]
4U3NX-ray3.20C8/c82-146[»]
4U3UX-ray2.90C8/c82-146[»]
4U4NX-ray3.10C8/c82-146[»]
4U4OX-ray3.60C8/c82-146[»]
4U4QX-ray3.00C8/c82-146[»]
4U4RX-ray2.80C8/c82-146[»]
4U4UX-ray3.00C8/c82-146[»]
4U4YX-ray3.20C8/c82-146[»]
4U4ZX-ray3.10C8/c82-146[»]
4U50X-ray3.20C8/c82-146[»]
4U51X-ray3.20C8/c82-146[»]
4U52X-ray3.00C8/c82-146[»]
4U53X-ray3.30C8/c82-146[»]
4U55X-ray3.20C8/c82-146[»]
4U56X-ray3.45C8/c82-146[»]
4U6FX-ray3.10C8/c82-146[»]
4V4Belectron microscopy11.70AM15-146[»]
4V6Ielectron microscopy8.80AM1-146[»]
4V7RX-ray4.00AL/CL1-146[»]
4V88X-ray3.00AS/CS1-146[»]
4V8Yelectron microscopy4.30AS1-146[»]
4V8Zelectron microscopy6.60AS1-146[»]
4V92electron microscopy3.70S2-141[»]
5DATX-ray3.15C8/c82-146[»]
5DC3X-ray3.25C8/c82-146[»]
5DGEX-ray3.45C8/c82-146[»]
5DGFX-ray3.30C8/c82-146[»]
5DGVX-ray3.10C8/c82-146[»]
5FCIX-ray3.40C8/c82-146[»]
5FCJX-ray3.10C8/c82-146[»]
5I4LX-ray3.10C8/c82-146[»]
5JUOelectron microscopy4.00PB1-146[»]
5JUPelectron microscopy3.50PB1-146[»]
5JUSelectron microscopy4.20PB1-146[»]
5JUTelectron microscopy4.00PB1-146[»]
5JUUelectron microscopy4.00PB1-146[»]
5LYBX-ray3.25C8/c82-146[»]
5M1Jelectron microscopy3.30S22-146[»]
5MC6electron microscopy3.80H1-146[»]
5MEIX-ray3.50T/c82-146[»]
5NDGX-ray3.70C8/c82-146[»]
5NDVX-ray3.30C8/c82-146[»]
5NDWX-ray3.70C8/c82-146[»]
5OBMX-ray3.40C8/c82-146[»]
5ON6X-ray3.10T/c82-146[»]
5TBWX-ray3.00T/c82-146[»]
5TGAX-ray3.30C8/c82-146[»]
5TGMX-ray3.50C8/c82-146[»]
5WLCelectron microscopy3.80L31-146[»]
6EMLelectron microscopy3.60H1-146[»]
6FAIelectron microscopy3.40S1-146[»]
ProteinModelPortaliP0CX55
SMRiP0CX55
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX55

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP0CX55
KOiK02964
OMAiHKRGQKV
OrthoDBiEOG092C5518

Family and domain databases

Gene3Di4.10.910.10, 1 hit
HAMAPiMF_01315 Ribosomal_S13_S18, 1 hit
InterProiView protein in InterPro
IPR027437 30s_Rbsml_prot_S13_C
IPR001892 Ribosomal_S13
IPR010979 Ribosomal_S13-like_H2TH
IPR018269 Ribosomal_S13_CS
PfamiView protein in Pfam
PF00416 Ribosomal_S13, 1 hit
PIRSFiPIRSF002134 Ribosomal_S13, 1 hit
SUPFAMiSSF46946 SSF46946, 1 hit
PROSITEiView protein in PROSITE
PS00646 RIBOSOMAL_S13_1, 1 hit
PS50159 RIBOSOMAL_S13_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLVVQEQGS FQHILRLLNT NVDGNIKIVY ALTTIKGVGR RYSNLVCKKA
60 70 80 90 100
DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQN DITDGKDYHT
110 120 130 140
LANNVESKLR DDLERLKKIR AHRGIRHFWG LRVRGQHTKT TGRRRA
Length:146
Mass (Da):17,038
Last modified:June 28, 2011 - v1
Checksum:i1F50BF9ADD20CA19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA Translation: AAB64891.1
BK006938 Genomic DNA Translation: DAA12285.1
PIRiS50886
RefSeqiNP_010738.1, NM_001180758.1
NP_013686.1, NM_001182384.1

Genome annotation databases

EnsemblFungiiYDR450W; YDR450W; YDR450W
YML026C; YML026C; YML026C
GeneIDi852061
854982
KEGGisce:YDR450W
sce:YML026C

Similar proteinsi

Entry informationi

Entry nameiRS18A_YEAST
AccessioniPrimary (citable) accession number: P0CX55
Secondary accession number(s): D6VT75, P35271
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 25, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health