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Protein

60S ribosomal protein L12-A

Gene

RPL12A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 68500 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for uL11 in yeast.Curated

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • ribosomal large subunit assembly Source: SGD
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30172-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L12-A1 Publication
Alternative name(s):
L15
Large ribosomal subunit protein uL11-A1 Publication
YL23
Gene namesi
Name:RPL12A1 Publication
Synonyms:RPL15B
Ordered Locus Names:YEL054C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

SGDiS000000780 RPL12A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67R → K: Abolishes monomethylation by RMT2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001044672 – 16560S ribosomal protein L12-AAdd BLAST164

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N-dimethylproline; by NTM12 Publications1
Modified residuei4N6,N6,N6-trimethyllysine; by RKM22 Publications1
Modified residuei11N6,N6,N6-trimethyllysine; by RKM21 Publication1
Modified residuei25PhosphoserineCombined sources1
Modified residuei38PhosphoserineCombined sources1
Modified residuei67N5-methylarginine; by RMT22 Publications1
Cross-linki130Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki146Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

It appears that the main modified species for L12 contains 6 methyl groups, 2 on Pro-2, 3 on Lys-4 and 1 on Arg-67. Although not reproduced with a second method, methylation at Lys-11 cannot be ruled out.4 Publications

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP0CX53
PaxDbiP0CX53
PRIDEiP0CX53

PTM databases

iPTMnetiP0CX53

Expressioni

Gene expression databases

ExpressionAtlasiP0CX53 differential

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi32476, 108 interactors
36675, 143 interactors
IntActiP0CX53, 4 interactors
MINTiP0CX53
STRINGi4932.YEL054C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-K13-143[»]
3J16electron microscopy-H1-165[»]
3J77electron microscopy6.20621-165[»]
3J78electron microscopy6.30621-165[»]
4V4Belectron microscopy11.70BK13-143[»]
4V6Ielectron microscopy8.80BJ1-165[»]
4V7Felectron microscopy8.70I1-165[»]
4V7RX-ray4.00DL1-165[»]
5DGEX-ray3.45m21-165[»]
5FL8electron microscopy9.50K1-165[»]
5JCSelectron microscopy9.50K1-165[»]
5JUOelectron microscopy4.00P1-165[»]
5JUPelectron microscopy3.50P1-165[»]
5JUSelectron microscopy4.20P1-165[»]
5JUTelectron microscopy4.00P1-165[»]
5JUUelectron microscopy4.00P1-165[»]
ProteinModelPortaliP0CX53
SMRiP0CX53
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX53

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000082125
InParanoidiP0CX53
KOiK02870
OMAiIKHNGNL
OrthoDBiEOG092C54AQ

Family and domain databases

CDDicd00349 Ribosomal_L11, 1 hit
Gene3Di1.10.10.250, 1 hit
3.30.1550.10, 1 hit
HAMAPiMF_00736 Ribosomal_L11, 1 hit
InterProiView protein in InterPro
IPR000911 Ribosomal_L11/L12
IPR036796 Ribosomal_L11/L12_N_sf
IPR020783 Ribosomal_L11_C
IPR036769 Ribosomal_L11_C_sf
IPR020785 Ribosomal_L11_CS
IPR020784 Ribosomal_L11_N
PANTHERiPTHR11661 PTHR11661, 1 hit
PfamiView protein in Pfam
PF00298 Ribosomal_L11, 1 hit
PF03946 Ribosomal_L11_N, 1 hit
SMARTiView protein in SMART
SM00649 RL11, 1 hit
SUPFAMiSSF46906 SSF46906, 1 hit
SSF54747 SSF54747, 1 hit
PROSITEiView protein in PROSITE
PS00359 RIBOSOMAL_L11, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKFDPNEV KYLYLRAVGG EVGASAALAP KIGPLGLSPK KVGEDIAKAT
60 70 80 90 100
KEFKGIKVTV QLKIQNRQAA ASVVPSASSL VITALKEPPR DRKKDKNVKH
110 120 130 140 150
SGNIQLDEII EIARQMRDKS FGRTLASVTK EILGTAQSVG CRVDFKNPHD
160
IIEGINAGEI EIPEN
Length:165
Mass (Da):17,823
Last modified:June 28, 2011 - v1
Checksum:i0B23A483781DFBE5
GO

Mass spectrometryi

Molecular mass is 17764.712 Da from positions 2 - 106. Determined by ESI. Monoisotopic mass with either 6 methylation modifications or 1 acetylation and 3 methylation modifications.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51519 Genomic DNA Translation: CAA35891.1
U18795 Genomic DNA Translation: AAB65033.1
AY693201 Genomic DNA Translation: AAT93220.1
BK006939 Genomic DNA Translation: DAA07600.1
PIRiS13665
RefSeqiNP_010706.3, NM_001180726.3
NP_010860.1, NM_001178869.1

Genome annotation databases

EnsemblFungiiYDR418W; YDR418W; YDR418W
YEL054C; YEL054C; YEL054C
GeneIDi852026
856656
KEGGisce:YDR418W
sce:YEL054C

Similar proteinsi

Entry informationi

Entry nameiRL12A_YEAST
AccessioniPrimary (citable) accession number: P0CX53
Secondary accession number(s): D3DLJ6, P05741, P17079
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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