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P0CX51

- RS16A_YEAST

UniProt

P0CX51 - RS16A_YEAST

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Protein

40S ribosomal protein S16-A

Gene

RPS16A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32835-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_248935. Ribosomal scanning and start codon recognition.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257608. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S16-A
Alternative name(s):
RP61R
Gene namesi
Name:RPS16A
Synonyms:RP61R
Ordered Locus Names:YMR143W
ORF Names:YM9375.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

SGDiS000004751. RPS16A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 14314240S ribosomal protein S16-APRO_0000111503Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications
Modified residuei34 – 341Phosphoserine2 Publications
Modified residuei61 – 611Phosphoserine1 Publication
Modified residuei70 – 701Phosphothreonine1 Publication
Modified residuei76 – 761Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP0CX51.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi31978. 151 interactions.
35319. 91 interactions.
IntActiP0CX51. 2 interactions.
MINTiMINT-8285666.

Structurei

Secondary structure

1
143
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 116Combined sources
Beta strandi17 – 248Combined sources
Beta strandi29 – 313Combined sources
Turni36 – 383Combined sources
Helixi45 – 5410Combined sources
Helixi57 – 604Combined sources
Beta strandi63 – 7210Combined sources
Helixi74 – 9522Combined sources
Helixi99 – 11214Combined sources
Beta strandi129 – 1357Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-I5-143[»]
1S1Helectron microscopy11.70I2-143[»]
1VW9electron microscopy6.10R1-143[»]
1VWVelectron microscopy6.10R1-143[»]
1VXWelectron microscopy6.20Q1-143[»]
1VXZelectron microscopy6.30Q1-143[»]
3IZBelectron microscopy-I1-143[»]
3O2ZX-ray4.00J1-143[»]
3O30X-ray4.00J1-143[»]
3U5CX-ray3.00Q1-143[»]
3U5GX-ray3.00Q1-143[»]
4BYLelectron microscopy4.30Q1-143[»]
4BYTelectron microscopy6.60Q1-143[»]
4CUYelectron microscopy3.70Q3-142[»]
ProteinModelPortaliP0CX51.
SMRiP0CX51. Positions 2-143.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX51.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S9P family.Curated

Phylogenomic databases

InParanoidiP0CX51.
KOiK02960.
OrthoDBiEOG7ZPNXQ.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
InterProiIPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000754. Ribosomal_S9.
IPR020574. Ribosomal_S9_CS.
[Graphical view]
PANTHERiPTHR21569. PTHR21569. 1 hit.
PfamiPF00380. Ribosomal_S9. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
PROSITEiPS00360. RIBOSOMAL_S9. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX51-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVE PEILRFKVYE
60 70 80 90 100
PLLLVGLDKF SNIDIRVRVT GGGHVSQVYA IRQAIAKGLV AYHQKYVDEQ
110 120 130 140
SKNELKKAFT SYDRTLLIAD SRRPEPKKFG GKGARSRFQK SYR
Length:143
Mass (Da):15,847
Last modified:June 28, 2011 - v1
Checksum:i46360374CD9730CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47071 Genomic DNA. Translation: CAA87357.1.
BK006946 Genomic DNA. Translation: DAA10039.1.
PIRiS67619.
RefSeqiNP_010200.1. NM_001180142.1.
NP_013863.2. NM_001182645.1.

Genome annotation databases

EnsemblFungiiYDL083C; YDL083C; YDL083C.
YMR143W; YMR143W; YMR143W.
GeneIDi851476.
855174.
KEGGisce:YDL083C.
sce:YMR143W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47071 Genomic DNA. Translation: CAA87357.1 .
BK006946 Genomic DNA. Translation: DAA10039.1 .
PIRi S67619.
RefSeqi NP_010200.1. NM_001180142.1.
NP_013863.2. NM_001182645.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K5X model - I 5-143 [» ]
1S1H electron microscopy 11.70 I 2-143 [» ]
1VW9 electron microscopy 6.10 R 1-143 [» ]
1VWV electron microscopy 6.10 R 1-143 [» ]
1VXW electron microscopy 6.20 Q 1-143 [» ]
1VXZ electron microscopy 6.30 Q 1-143 [» ]
3IZB electron microscopy - I 1-143 [» ]
3O2Z X-ray 4.00 J 1-143 [» ]
3O30 X-ray 4.00 J 1-143 [» ]
3U5C X-ray 3.00 Q 1-143 [» ]
3U5G X-ray 3.00 Q 1-143 [» ]
4BYL electron microscopy 4.30 Q 1-143 [» ]
4BYT electron microscopy 6.60 Q 1-143 [» ]
4CUY electron microscopy 3.70 Q 3-142 [» ]
ProteinModelPortali P0CX51.
SMRi P0CX51. Positions 2-143.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31978. 151 interactions.
35319. 91 interactions.
IntActi P0CX51. 2 interactions.
MINTi MINT-8285666.

Proteomic databases

MaxQBi P0CX51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL083C ; YDL083C ; YDL083C .
YMR143W ; YMR143W ; YMR143W .
GeneIDi 851476.
855174.
KEGGi sce:YDL083C.
sce:YMR143W.

Organism-specific databases

SGDi S000004751. RPS16A.

Phylogenomic databases

InParanoidi P0CX51.
KOi K02960.
OrthoDBi EOG7ZPNXQ.

Enzyme and pathway databases

BioCyci YEAST:G3O-32835-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_248935. Ribosomal scanning and start codon recognition.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257608. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

EvolutionaryTracei P0CX51.
NextBioi 968780.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
InterProi IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000754. Ribosomal_S9.
IPR020574. Ribosomal_S9_CS.
[Graphical view ]
PANTHERi PTHR21569. PTHR21569. 1 hit.
Pfami PF00380. Ribosomal_S9. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
PROSITEi PS00360. RIBOSOMAL_S9. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
    Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
    J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-26, ACETYLATION AT SER-2 BY NATA.
  4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  5. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61; THR-70 AND SER-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 5-143, ELECTRON MICROSCOPY.
  13. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.

Entry informationi

Entry nameiRS16A_YEAST
AccessioniPrimary (citable) accession number: P0CX51
Secondary accession number(s): D6VRR6, P26787, P40213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 26, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 33800 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S16 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3