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Protein

40S ribosomal protein S16-A

Gene

RPS16A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 33800 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for uS9 in yeast.Curated

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32835-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S16-A1 Publication
Alternative name(s):
RP61R
Small ribosomal subunit protein uS9-A1 Publication
Gene namesi
Name:RPS16A1 Publication
Synonyms:RP61R
Ordered Locus Names:YMR143W
ORF Names:YM9375.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

SGDiS000004751. RPS16A.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001115032 – 14340S ribosomal protein S16-AAdd BLAST142

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine2 Publications1
Cross-linki30Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei34PhosphoserineCombined sources1
Cross-linki47Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki59Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei61PhosphoserineCombined sources1
Modified residuei70PhosphothreonineCombined sources1
Modified residuei76PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP0CX51.
PRIDEiP0CX51.
TopDownProteomicsiP0CX51.

PTM databases

iPTMnetiP0CX51.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX51. differential.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi31978. 247 interactors.
35319. 257 interactors.
IntActiP0CX51. 2 interactors.
MINTiMINT-8285666.

Structurei

Secondary structure

1143
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 13Combined sources9
Beta strandi16 – 26Combined sources11
Beta strandi29 – 31Combined sources3
Turni36 – 38Combined sources3
Beta strandi40 – 42Combined sources3
Turni43 – 45Combined sources3
Helixi46 – 54Combined sources9
Helixi57 – 59Combined sources3
Beta strandi63 – 72Combined sources10
Helixi76 – 95Combined sources20
Helixi99 – 112Combined sources14
Beta strandi114 – 118Combined sources5
Beta strandi129 – 135Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-I5-143[»]
3J6Xelectron microscopy6.10161-143[»]
3J6Yelectron microscopy6.10161-143[»]
3J77electron microscopy6.20161-143[»]
3J78electron microscopy6.30161-143[»]
3V88X-ray3.00Q1-143[»]
4U3MX-ray3.00C6/c62-143[»]
4U3NX-ray3.20C6/c62-143[»]
4U3UX-ray2.90C6/c62-143[»]
4U4NX-ray3.10C6/c62-143[»]
4U4OX-ray3.60C6/c62-143[»]
4U4QX-ray3.00C6/c62-143[»]
4U4RX-ray2.80C6/c62-143[»]
4U4UX-ray3.00C6/c62-143[»]
4U4YX-ray3.20C6/c62-143[»]
4U4ZX-ray3.10C6/c62-143[»]
4U50X-ray3.20C6/c62-143[»]
4U51X-ray3.20C6/c62-143[»]
4U52X-ray3.00C6/c62-143[»]
4U53X-ray3.30C6/c62-143[»]
4U55X-ray3.20C6/c62-143[»]
4U56X-ray3.45C6/c62-143[»]
4U6FX-ray3.10C6/c62-143[»]
4V4Belectron microscopy11.70AI2-143[»]
4V6Ielectron microscopy8.80AI1-143[»]
4V7RX-ray4.00AJ/CJ1-143[»]
4V88X-ray3.00AQ/CQ1-143[»]
4V8Yelectron microscopy4.30AQ1-143[»]
4V8Zelectron microscopy6.60AQ1-143[»]
4V92electron microscopy3.70Q3-142[»]
5DATX-ray3.15C6/c62-143[»]
5DC3X-ray3.25C6/c62-143[»]
5DGEX-ray3.45C6/c62-143[»]
5DGFX-ray3.30C6/c62-143[»]
5DGVX-ray3.10C6/c62-143[»]
5FCIX-ray3.40C6/c62-143[»]
5FCJX-ray3.10C6/c62-143[»]
5I4LX-ray3.10C6/c62-143[»]
5JPQelectron microscopy7.30x1-143[»]
5JUOelectron microscopy4.00NB1-143[»]
5JUPelectron microscopy3.50NB1-143[»]
5JUSelectron microscopy4.20NB1-143[»]
5JUTelectron microscopy4.00NB1-143[»]
5JUUelectron microscopy4.00NB1-143[»]
5LYBX-ray3.25C6/c62-143[»]
5M1Jelectron microscopy3.30Q23-143[»]
5MC6electron microscopy3.80F1-143[»]
5TGAX-ray3.30C6/c62-143[»]
5TGMX-ray3.50C6/c62-143[»]
5TZSelectron microscopy5.10C1-143[»]
5WYJelectron microscopy8.70SR1-143[»]
5WYKelectron microscopy4.50SR1-143[»]
ProteinModelPortaliP0CX51.
SMRiP0CX51.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX51.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP0CX51.
KOiK02960.
OMAiHVSQIYA.
OrthoDBiEOG092C5DBH.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
InterProiView protein in InterPro
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000754. Ribosomal_S9.
IPR020574. Ribosomal_S9_CS.
PANTHERiPTHR21569. PTHR21569. 1 hit.
PfamiView protein in Pfam
PF00380. Ribosomal_S9. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
PROSITEiView protein in PROSITE
PS00360. RIBOSOMAL_S9. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVE PEILRFKVYE
60 70 80 90 100
PLLLVGLDKF SNIDIRVRVT GGGHVSQVYA IRQAIAKGLV AYHQKYVDEQ
110 120 130 140
SKNELKKAFT SYDRTLLIAD SRRPEPKKFG GKGARSRFQK SYR
Length:143
Mass (Da):15,847
Last modified:June 28, 2011 - v1
Checksum:i46360374CD9730CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47071 Genomic DNA. Translation: CAA87357.1.
BK006946 Genomic DNA. Translation: DAA10039.1.
PIRiS67619.
RefSeqiNP_010200.1. NM_001180142.1.
NP_013863.2. NM_001182645.1.

Genome annotation databases

EnsemblFungiiYDL083C; YDL083C; YDL083C.
YMR143W; YMR143W; YMR143W.
GeneIDi851476.
855174.
KEGGisce:YDL083C.
sce:YMR143W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47071 Genomic DNA. Translation: CAA87357.1.
BK006946 Genomic DNA. Translation: DAA10039.1.
PIRiS67619.
RefSeqiNP_010200.1. NM_001180142.1.
NP_013863.2. NM_001182645.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Xmodel-I5-143[»]
3J6Xelectron microscopy6.10161-143[»]
3J6Yelectron microscopy6.10161-143[»]
3J77electron microscopy6.20161-143[»]
3J78electron microscopy6.30161-143[»]
3V88X-ray3.00Q1-143[»]
4U3MX-ray3.00C6/c62-143[»]
4U3NX-ray3.20C6/c62-143[»]
4U3UX-ray2.90C6/c62-143[»]
4U4NX-ray3.10C6/c62-143[»]
4U4OX-ray3.60C6/c62-143[»]
4U4QX-ray3.00C6/c62-143[»]
4U4RX-ray2.80C6/c62-143[»]
4U4UX-ray3.00C6/c62-143[»]
4U4YX-ray3.20C6/c62-143[»]
4U4ZX-ray3.10C6/c62-143[»]
4U50X-ray3.20C6/c62-143[»]
4U51X-ray3.20C6/c62-143[»]
4U52X-ray3.00C6/c62-143[»]
4U53X-ray3.30C6/c62-143[»]
4U55X-ray3.20C6/c62-143[»]
4U56X-ray3.45C6/c62-143[»]
4U6FX-ray3.10C6/c62-143[»]
4V4Belectron microscopy11.70AI2-143[»]
4V6Ielectron microscopy8.80AI1-143[»]
4V7RX-ray4.00AJ/CJ1-143[»]
4V88X-ray3.00AQ/CQ1-143[»]
4V8Yelectron microscopy4.30AQ1-143[»]
4V8Zelectron microscopy6.60AQ1-143[»]
4V92electron microscopy3.70Q3-142[»]
5DATX-ray3.15C6/c62-143[»]
5DC3X-ray3.25C6/c62-143[»]
5DGEX-ray3.45C6/c62-143[»]
5DGFX-ray3.30C6/c62-143[»]
5DGVX-ray3.10C6/c62-143[»]
5FCIX-ray3.40C6/c62-143[»]
5FCJX-ray3.10C6/c62-143[»]
5I4LX-ray3.10C6/c62-143[»]
5JPQelectron microscopy7.30x1-143[»]
5JUOelectron microscopy4.00NB1-143[»]
5JUPelectron microscopy3.50NB1-143[»]
5JUSelectron microscopy4.20NB1-143[»]
5JUTelectron microscopy4.00NB1-143[»]
5JUUelectron microscopy4.00NB1-143[»]
5LYBX-ray3.25C6/c62-143[»]
5M1Jelectron microscopy3.30Q23-143[»]
5MC6electron microscopy3.80F1-143[»]
5TGAX-ray3.30C6/c62-143[»]
5TGMX-ray3.50C6/c62-143[»]
5TZSelectron microscopy5.10C1-143[»]
5WYJelectron microscopy8.70SR1-143[»]
5WYKelectron microscopy4.50SR1-143[»]
ProteinModelPortaliP0CX51.
SMRiP0CX51.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31978. 247 interactors.
35319. 257 interactors.
IntActiP0CX51. 2 interactors.
MINTiMINT-8285666.

PTM databases

iPTMnetiP0CX51.

Proteomic databases

MaxQBiP0CX51.
PRIDEiP0CX51.
TopDownProteomicsiP0CX51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL083C; YDL083C; YDL083C.
YMR143W; YMR143W; YMR143W.
GeneIDi851476.
855174.
KEGGisce:YDL083C.
sce:YMR143W.

Organism-specific databases

SGDiS000004751. RPS16A.

Phylogenomic databases

InParanoidiP0CX51.
KOiK02960.
OMAiHVSQIYA.
OrthoDBiEOG092C5DBH.

Enzyme and pathway databases

BioCyciYEAST:G3O-32835-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiP0CX51.
PROiPR:P0CX51.

Gene expression databases

ExpressionAtlasiP0CX51. differential.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
InterProiView protein in InterPro
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000754. Ribosomal_S9.
IPR020574. Ribosomal_S9_CS.
PANTHERiPTHR21569. PTHR21569. 1 hit.
PfamiView protein in Pfam
PF00380. Ribosomal_S9. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
PROSITEiView protein in PROSITE
PS00360. RIBOSOMAL_S9. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRS16A_YEAST
AccessioniPrimary (citable) accession number: P0CX51
Secondary accession number(s): D6VRR6, P26787, P40213
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: June 7, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.