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Protein

60S ribosomal protein L18-B

Gene

RPL18B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 63700 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eL18 in yeast.Curated

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-33289-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L18-B1 Publication
Alternative name(s):
Large ribosomal subunit protein eL18-B1 Publication
RP28
Gene namesi
Name:RPL18B1 Publication
Synonyms:RP28B
Ordered Locus Names:YNL301C
ORF Names:N0425
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

SGDiS000005245. RPL18B.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00004097712 – 18660S ribosomal protein L18-BAdd BLAST185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50N6,N6,N6-trimethyllysine1 Publication1
Cross-linki116Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

PRIDEiP0CX50.

PTM databases

iPTMnetiP0CX50.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX50. differential.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102). eL18 interacts with NAP1 (PubMed:18086883).1 Publication2 Publications

Protein-protein interaction databases

BioGridi34281. 75 interactors.
35537. 116 interactors.
IntActiP0CX50. 1 interactor.
MINTiMINT-533994.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V8Telectron microscopy8.10Q1-186[»]
ProteinModelPortaliP0CX50.
SMRiP0CX50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000012976.
InParanoidiP0CX50.
KOiK02883.
OrthoDBiEOG092C592A.

Family and domain databases

InterProiView protein in InterPro
IPR000039. Ribosomal_L18e.
IPR021131. Ribosomal_L18e/L15P.
IPR021132. Ribosomal_L18e_CS.
PANTHERiPTHR10934. PTHR10934. 1 hit.
PfamiView protein in Pfam
PF17135. Ribosomal_L18. 1 hit.
SUPFAMiSSF52080. SSF52080. 1 hit.
PROSITEiView protein in PROSITE
PS01106. RIBOSOMAL_L18E. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK
60 70 80 90 100
ALFLSKINRP PVSVSRIARA LKQEGAANKT VVVVGTVTDD ARIFEFPKTT
110 120 130 140 150
VAALRFTAGA RAKIVKAGGE CITLDQLAVR APKGQNTLIL RGPRNSREAV
160 170 180
RHFGMGPHKG KAPRILSTGR KFERARGRRR SKGFKV
Length:186
Mass (Da):20,563
Last modified:June 28, 2011 - v1
Checksum:iD097B187F369EACD
GO

Mass spectrometryi

Molecular mass is 20419.431 Da from positions 2 - 186. Determined by ESI. Monoisotopic mass.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01100 Genomic DNA. Translation: CAA25574.1.
U23084 Genomic DNA. Translation: AAC49097.1.
Z71577 Genomic DNA. Translation: CAA96219.1.
BK006947 Genomic DNA. Translation: DAA10259.1.
PIRiS05867. R5BY8E.
RefSeqiNP_014098.1. NM_001183139.1.
NP_014521.1. NM_001183374.1.

Genome annotation databases

EnsemblFungiiYNL301C; YNL301C; YNL301C.
YOL120C; YOL120C; YOL120C.
GeneIDi854029.
855415.
KEGGisce:YNL301C.
sce:YOL120C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01100 Genomic DNA. Translation: CAA25574.1.
U23084 Genomic DNA. Translation: AAC49097.1.
Z71577 Genomic DNA. Translation: CAA96219.1.
BK006947 Genomic DNA. Translation: DAA10259.1.
PIRiS05867. R5BY8E.
RefSeqiNP_014098.1. NM_001183139.1.
NP_014521.1. NM_001183374.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V8Telectron microscopy8.10Q1-186[»]
ProteinModelPortaliP0CX50.
SMRiP0CX50.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34281. 75 interactors.
35537. 116 interactors.
IntActiP0CX50. 1 interactor.
MINTiMINT-533994.

PTM databases

iPTMnetiP0CX50.

Proteomic databases

PRIDEiP0CX50.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL301C; YNL301C; YNL301C.
YOL120C; YOL120C; YOL120C.
GeneIDi854029.
855415.
KEGGisce:YNL301C.
sce:YOL120C.

Organism-specific databases

SGDiS000005245. RPL18B.

Phylogenomic databases

GeneTreeiENSGT00390000012976.
InParanoidiP0CX50.
KOiK02883.
OrthoDBiEOG092C592A.

Enzyme and pathway databases

BioCyciYEAST:G3O-33289-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiPR:P0CX50.

Gene expression databases

ExpressionAtlasiP0CX50. differential.

Family and domain databases

InterProiView protein in InterPro
IPR000039. Ribosomal_L18e.
IPR021131. Ribosomal_L18e/L15P.
IPR021132. Ribosomal_L18e_CS.
PANTHERiPTHR10934. PTHR10934. 1 hit.
PfamiView protein in Pfam
PF17135. Ribosomal_L18. 1 hit.
SUPFAMiSSF52080. SSF52080. 1 hit.
PROSITEiView protein in PROSITE
PS01106. RIBOSOMAL_L18E. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRL18B_YEAST
AccessioniPrimary (citable) accession number: P0CX50
Secondary accession number(s): D6W0P3, P07279
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 12, 2017
This is version 50 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.