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P0CX49

- RL18A_YEAST

UniProt

P0CX49 - RL18A_YEAST

Protein

60S ribosomal protein L18-A

Gene

RPL18A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 25 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33516-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L18-A
    Alternative name(s):
    RP28
    Gene namesi
    Name:RPL18A
    Synonyms:RP28A
    Ordered Locus Names:YOL120C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL120c.
    SGDiS000005480. RPL18A.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 18618560S ribosomal protein L18-APRO_0000132784Add
    BLAST

    Proteomic databases

    MaxQBiP0CX49.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with NAP1.2 Publications

    Protein-protein interaction databases

    BioGridi34281. 80 interactions.
    35537. 80 interactions.
    IntActiP0CX49. 1 interaction.
    MINTiMINT-8285571.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Ymodel-O22-141[»]
    1S1Ielectron microscopy11.70O22-141[»]
    1VW8electron microscopy6.10R1-186[»]
    1VWUelectron microscopy6.10R1-186[»]
    3IZSelectron microscopy8.80R1-186[»]
    3J65electron microscopy8.70Q1-186[»]
    3O58X-ray4.00R1-186[»]
    3O5HX-ray4.00R1-186[»]
    3U5EX-ray3.00Q1-186[»]
    3U5IX-ray3.00Q1-186[»]
    4BYNelectron microscopy4.30Q2-186[»]
    4BYUelectron microscopy6.60Q2-186[»]
    4CUWelectron microscopy3.70Q1-186[»]
    ProteinModelPortaliP0CX49.
    SMRiP0CX49. Positions 2-186.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0CX49.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L18e family.Curated

    Phylogenomic databases

    KOiK02883.
    OrthoDBiEOG790GC3.

    Family and domain databases

    InterProiIPR000039. Ribosomal_L18e.
    IPR021131. Ribosomal_L18e/L15P.
    IPR021132. Ribosomal_L18e_CS.
    [Graphical view]
    PANTHERiPTHR10934. PTHR10934. 1 hit.
    PfamiPF00828. Ribosomal_L18e. 1 hit.
    [Graphical view]
    SUPFAMiSSF52080. SSF52080. 1 hit.
    PROSITEiPS01106. RIBOSOMAL_L18E. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CX49-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK    50
    ALFLSKINRP PVSVSRIARA LKQEGAANKT VVVVGTVTDD ARIFEFPKTT 100
    VAALRFTAGA RAKIVKAGGE CITLDQLAVR APKGQNTLIL RGPRNSREAV 150
    RHFGMGPHKG KAPRILSTGR KFERARGRRR SKGFKV 186
    Length:186
    Mass (Da):20,563
    Last modified:June 28, 2011 - v1
    Checksum:iD097B187F369EACD
    GO

    Mass spectrometryi

    Molecular mass is 20419.431 Da from positions 2 - 186. Determined by ESI. Monoisotopic mass.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01099 Genomic DNA. Translation: CAA25573.1.
    X02635 Genomic DNA. Translation: CAA26481.1.
    X95258 Genomic DNA. Translation: CAA64550.1.
    Z74862 Genomic DNA. Translation: CAA99139.1.
    BK006948 Genomic DNA. Translation: DAA10664.1.
    PIRiS05867. R5BY8E.
    RefSeqiNP_014098.1. NM_001183139.1.
    NP_014521.1. NM_001183374.1.

    Genome annotation databases

    EnsemblFungiiYNL301C; YNL301C; YNL301C.
    YOL120C; YOL120C; YOL120C.
    GeneIDi854029.
    855415.
    KEGGisce:YNL301C.
    sce:YOL120C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01099 Genomic DNA. Translation: CAA25573.1 .
    X02635 Genomic DNA. Translation: CAA26481.1 .
    X95258 Genomic DNA. Translation: CAA64550.1 .
    Z74862 Genomic DNA. Translation: CAA99139.1 .
    BK006948 Genomic DNA. Translation: DAA10664.1 .
    PIRi S05867. R5BY8E.
    RefSeqi NP_014098.1. NM_001183139.1.
    NP_014521.1. NM_001183374.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5Y model - O 22-141 [» ]
    1S1I electron microscopy 11.70 O 22-141 [» ]
    1VW8 electron microscopy 6.10 R 1-186 [» ]
    1VWU electron microscopy 6.10 R 1-186 [» ]
    3IZS electron microscopy 8.80 R 1-186 [» ]
    3J65 electron microscopy 8.70 Q 1-186 [» ]
    3O58 X-ray 4.00 R 1-186 [» ]
    3O5H X-ray 4.00 R 1-186 [» ]
    3U5E X-ray 3.00 Q 1-186 [» ]
    3U5I X-ray 3.00 Q 1-186 [» ]
    4BYN electron microscopy 4.30 Q 2-186 [» ]
    4BYU electron microscopy 6.60 Q 2-186 [» ]
    4CUW electron microscopy 3.70 Q 1-186 [» ]
    ProteinModelPortali P0CX49.
    SMRi P0CX49. Positions 2-186.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34281. 80 interactions.
    35537. 80 interactions.
    IntActi P0CX49. 1 interaction.
    MINTi MINT-8285571.

    Proteomic databases

    MaxQBi P0CX49.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL301C ; YNL301C ; YNL301C .
    YOL120C ; YOL120C ; YOL120C .
    GeneIDi 854029.
    855415.
    KEGGi sce:YNL301C.
    sce:YOL120C.

    Organism-specific databases

    CYGDi YOL120c.
    SGDi S000005480. RPL18A.

    Phylogenomic databases

    KOi K02883.
    OrthoDBi EOG790GC3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33516-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P0CX49.
    NextBioi 975577.

    Family and domain databases

    InterProi IPR000039. Ribosomal_L18e.
    IPR021131. Ribosomal_L18e/L15P.
    IPR021132. Ribosomal_L18e_CS.
    [Graphical view ]
    PANTHERi PTHR10934. PTHR10934. 1 hit.
    Pfami PF00828. Ribosomal_L18e. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52080. SSF52080. 1 hit.
    PROSITEi PS01106. RIBOSOMAL_L18E. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and organization of two linked ribosomal protein genes in yeast."
      Molenaar C.M.T., Woudt L.P., Jansen A.E.M., Mager W.H., Planta R.J., Donovan D.M., Pearson N.J.
      Nucleic Acids Res. 12:7345-7358(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames."
      Lafuente M.J., Gamo F.-J., Gancedo C.
      Yeast 12:1041-1045(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    6. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
      Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
      Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Phosphorylation by casein kinase 2 regulates Nap1 localization and function."
      Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F.
      Mol. Cell. Biol. 28:1313-1325(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NAP1, IDENTIFICATION BY MASS SPECTROMETRY.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 22-141, ELECTRON MICROSCOPY.
    12. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 22-141, ELECTRON MICROSCOPY.
    13. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (8.80 ANGSTROMS).
    14. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS).
    15. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

    Entry informationi

    Entry nameiRL18A_YEAST
    AccessioniPrimary (citable) accession number: P0CX49
    Secondary accession number(s): D6W0P3, P07279
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 2 genes for L18 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3