P0CX49 (RL18A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L18-A

Alternative name(s):
RP28

Gene names

Name:	RPL18A
Synonyms:	RP28A
Ordered Locus Names:	YOL120C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with NAP1. Ref.5 Ref.9
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 genes for L18 in yeast.
Sequence similarities	Belongs to the ribosomal protein L18e family.
Mass spectrometry	Molecular mass is 20419.431 Da from positions 2 - 186. Determined by ESI. Monoisotopic mass. Ref.6

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.5. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.5. Source: SGD
Molecular_function	structural constituent of ribosome Traceable author statement Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 186	185	60S ribosomal protein L18-A		PRO_0000132784
Amino acid modifications
	Modified residue	86	1	Phosphothreonine Ref.8
	Modified residue	167	1	Phosphoserine Ref.7 Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P0CX49 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: D097B187F369EACD	FASTA	186	20,563
	10 20 30 40 50 60 MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK ALFLSKINRP 70 80 90 100 110 120 PVSVSRIARA LKQEGAANKT VVVVGTVTDD ARIFEFPKTT VAALRFTAGA RAKIVKAGGE 130 140 150 160 170 180 CITLDQLAVR APKGQNTLIL RGPRNSREAV RHFGMGPHKG KAPRILSTGR KFERARGRRR SKGFKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and organization of two linked ribosomal protein genes in yeast." Molenaar C.M.T., Woudt L.P., Jansen A.E.M., Mager W.H., Planta R.J., Donovan D.M., Pearson N.J. Nucleic Acids Res. 12:7345-7358(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames." Lafuente M.J., Gamo F.-J., Gancedo C. Yeast 12:1041-1045(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[6]	"Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR." Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D. Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-86 AND SER-167, MASS SPECTROMETRY.
[9]	"Phosphorylation by casein kinase 2 regulates Nap1 localization and function." Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F. Mol. Cell. Biol. 28:1313-1325(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NAP1, MASS SPECTROMETRY.
[10]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 22-141, ELECTRON MICROSCOPY.
[11]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 22-141, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X01099 Genomic DNA. Translation: CAA25573.1.
X02635 Genomic DNA. Translation: CAA26481.1.
X95258 Genomic DNA. Translation: CAA64550.1.
Z74862 Genomic DNA. Translation: CAA99139.1.
BK006948 Genomic DNA. Translation: DAA10664.1.

PIR

R5BY8E. S05867.

RefSeq

NP_014098.1. NM_001183139.1.
NP_014521.1. NM_001183374.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	O	22-141	[»]
1S1I	electron microscopy	11.70	O	22-141	[»]
3IZS	electron microscopy	-	R	1-186	[»]
3O58	X-ray	4.00	R	1-186	[»]
3O5H	X-ray	4.00	R	1-186	[»]
3U5E	X-ray	3.00	Q	1-186	[»]
3U5I	X-ray	3.00	Q	1-186	[»]

ProteinModelPortal

P0CX49.

SMR

P0CX49. Positions 2-186.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YNL301C; YNL301C; YNL301C.
YOL120C; YOL120C; YOL120C.

GeneID

854029.
855415.

KEGG

sce:YNL301C.
sce:YOL120C.

Organism-specific databases

CYGD

YOL120c.

SGD

S000005480. RPL18A.

Phylogenomic databases

KO

K02883.

Gene expression databases

ArrayExpress

P0CX49.

GermOnline

YOL120C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000039. Ribosomal_L18e.
IPR021131. Ribosomal_L18e/L15P.
IPR021132. Ribosomal_L18e_CS.
[Graphical view]

PANTHER

PTHR10934. PTHR10934. 1 hit.

Pfam

PF00828. Ribosomal_L18e. 1 hit.
[Graphical view]

SUPFAM

SSF52080. Ribosomal_L18e/L15. 1 hit.

PROSITE

PS01106. RIBOSOMAL_L18E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0CX49.

NextBio

975577.

Entry information

Entry name

RL18A_YEAST

Accession

Primary (citable) accession number: P0CX49
Secondary accession number(s): D6W0P3, P07279

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program