P0CX48 (RS11B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S11-B

Alternative name(s):
RP41
S18
YS12

Gene names

Name:	RPS11B
Synonyms:	RPS18B
Ordered Locus Names:	YBR048W
ORF Names:	YBR0501

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm Ref.7.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Miscellaneous	Present with 1200 molecules/cell in log phase SD medium. There are 2 genes for S11 in yeast.
Sequence similarities	Belongs to the ribosomal protein S17P family.
Sequence caution	The sequence CAA86390.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred by curator PubMed 6814480. Source: SGD maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from genetic interaction PubMed 16246728. Source: SGD ribosomal small subunit assembly Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	90S preribosome Inferred from direct assay PubMed 12150911. Source: SGD cytosolic small ribosomal subunit Inferred from direct assay PubMed 6814480. Source: SGD
Molecular_function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from direct assay PubMed 6814480. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 156	155	40S ribosomal protein S11-B		PRO_0000409770
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.5
	Modified residue	74	1	Phosphothreonine Ref.9
Experimental info
	Sequence conflict	12 – 13	2	AF → FA AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P0CX48 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 6F7825FAE17EB084	FASTA	156	17,749
	10 20 30 40 50 60 MSTELTVQSE RAFQKQPHIF NNPKVKTSKR TKRWYKNAGL GFKTPKTAIE GSYIDKKCPF 70 80 90 100 110 120 TGLVSIRGKI LTGTVVSTKM HRTIVIRRAY LHYIPKYNRY EKRHKNVPVH VSPAFRVQVG 130 140 150 DIVTVGQCRP ISKTVRFNVV KVSAAAGKAN KQFAKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reduced dosage of genes encoding ribosomal protein S18 suppresses a mitochondrial initiation codon mutation in Saccharomyces cerevisiae." Folley L.S., Fox T.D. Genetics 137:369-379(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Sequence and analysis of 24 kb on chromosome II of Saccharomyces cerevisiae." Aljinovic G., Pohl T.M. Yeast 11:475-479(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21, ACETYLATION AT SER-2 BY NATA.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-74, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L17004 Unassigned DNA. Translation: AAC37410.1. Z46260 Genomic DNA. Translation: CAA86390.1. Sequence problems. Z35917 Genomic DNA. Translation: CAA84990.1. Z35918 Genomic DNA. Translation: CAA84991.1. BK006936 Genomic DNA. Translation: DAA07168.1.
PIR	S41784.
RefSeq	NP_009604.1. NM_001178396.1. NP_010308.3. NM_001180333.3.
3D structure databases
ProteinModelPortal	P0CX48.
SMR	P0CX48. Positions 2-156.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR048W; YBR048W; YBR048W. YDR025W; YDR025W; YDR025W.
GeneID	851589. 852337.
KEGG	sce:YBR048W. sce:YDR025W. sce:YDR030C.
Organism-specific databases
CYGD	YBR048w.
SGD	S000000252. RPS11B.
Phylogenomic databases
GeneTree	ENSGT00390000002732.
KO	K02949. K10570.
Gene expression databases
ArrayExpress	P0CX48.
GermOnline	YBR048W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
InterPro	IPR012340. NA-bd_OB-fold. IPR000266. Ribosomal_S17. IPR019978. Ribosomal_S17_archaeal. IPR019979. Ribosomal_S17_CS. [Graphical view]
PANTHER	PTHR10744. PTHR10744. 1 hit.
Pfam	PF00366. Ribosomal_S17. 1 hit. [Graphical view]
PRINTS	PR00973. RIBOSOMALS17.
ProDom	PD001295. Ribosomal_S17. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR03630. arch_S17P. 1 hit.
PROSITE	PS00056. RIBOSOMAL_S17. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969066.

Entry information

Entry name

RS11B_YEAST

Accession

Primary (citable) accession number: P0CX48
Secondary accession number(s): D6VQ48, O11852, P26781

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program