P0CX47 (RS11A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S11-A

Alternative name(s):
RP41
S18
YS12

Gene names

Name:	RPS11A
Synonyms:	RPS18A
Ordered Locus Names:	YDR025W
ORF Names:	YD9813.03

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm Ref.8.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Miscellaneous	There are 2 genes for S11 in yeast.
Sequence similarities	Belongs to the ribosomal protein S17P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred by curator PubMed 6814480. Source: SGD maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from genetic interaction PubMed 16246728. Source: SGD ribosomal small subunit assembly Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	90S preribosome Inferred from direct assay PubMed 12150911. Source: SGD cytosolic small ribosomal subunit Inferred from direct assay PubMed 6814480. Source: SGD
Molecular_function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from direct assay PubMed 6814480. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5 Ref.7

Chain

2 – 156

155

40S ribosomal protein S11-A

PRO_0000128523

Amino acid modifications

Modified residue

2

1

N-acetylserine Ref.5 Ref.7

Modified residue

74

1

Phosphothreonine Ref.9

Experimental info

Sequence conflict

12 – 13

2

AF → FA AA sequence Ref.5

Secondary structure

1

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156

Helix Strand Turn

Details...

Beta strand

10 – 12

3

Beta strand

23 – 25

3

Beta strand

28 – 31

4

Helix

46 – 50

5

Turn

59 – 61

3

Beta strand

66 – 77

12

Beta strand

83 – 94

12

Turn

95 – 98

4

Beta strand

99 – 110

12

Beta strand

122 – 132

11

Beta strand

135 – 142

8

Beta strand

147 – 149

3

Helix

150 – 152

3

Sequences

	Sequence		Length	Mass (Da)
	P0CX47 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 6F7825FAE17EB084	FASTA	156	17,749
	10 20 30 40 50 60 MSTELTVQSE RAFQKQPHIF NNPKVKTSKR TKRWYKNAGL GFKTPKTAIE GSYIDKKCPF 70 80 90 100 110 120 TGLVSIRGKI LTGTVVSTKM HRTIVIRRAY LHYIPKYNRY EKRHKNVPVH VSPAFRVQVG 130 140 150 DIVTVGQCRP ISKTVRFNVV KVSAAAGKAN KQFAKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reduced dosage of genes encoding ribosomal protein S18 suppresses a mitochondrial initiation codon mutation in Saccharomyces cerevisiae." Folley L.S., Fox T.D. Genetics 137:369-379(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames." Eide L.G., Sander C., Prydz H. Yeast 12:1085-1090(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21, ACETYLATION AT SER-2 BY NATA.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-74, MASS SPECTROMETRY.
[10]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 69-144, ELECTRON MICROSCOPY.
[11]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 69-144, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L15408 Unassigned DNA. Translation: AAC37411.1.
X95966 Genomic DNA. Translation: CAA65218.1.
Z47814 Genomic DNA. Translation: CAA87804.1.
Z74321 Genomic DNA. Translation: CAA98846.1.
BK006938 Genomic DNA. Translation: DAA11870.1.

PIR

S41784.

RefSeq

NP_009604.1. NM_001178396.1.
NP_010308.3. NM_001180333.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5X	model	-	Q	69-144	[»]
1S1H	electron microscopy	11.70	Q	69-144	[»]
3IZB	electron microscopy	-	P	1-156	[»]
3O2Z	X-ray	4.00	F	1-156	[»]
3O30	X-ray	4.00	F	1-156	[»]
3U5C	X-ray	3.00	L	1-156	[»]
3U5G	X-ray	3.00	L	1-156	[»]

ProteinModelPortal

P0CX47.

SMR

P0CX47. Positions 2-156.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBR048W; YBR048W; YBR048W.
YDR025W; YDR025W; YDR025W.

GeneID

851589.
852337.

KEGG

sce:YBR048W.
sce:YDR025W.
sce:YDR030C.

Organism-specific databases

CYGD

YDR025w.

SGD

S000002432. RPS11A.

Phylogenomic databases

KO

K02949.
K10570.

Gene expression databases

ArrayExpress

P0CX47.

GermOnline

YDR025W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

2.40.50.140. 1 hit.

InterPro

IPR012340. NA-bd_OB-fold.
IPR000266. Ribosomal_S17.
IPR019978. Ribosomal_S17_archaeal.
IPR019979. Ribosomal_S17_CS.
[Graphical view]

PANTHER

PTHR10744. PTHR10744. 1 hit.

Pfam

PF00366. Ribosomal_S17. 1 hit.
[Graphical view]

PRINTS

PR00973. RIBOSOMALS17.

ProDom

PD001295. Ribosomal_S17. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.

TIGRFAMs

TIGR03630. arch_S17P. 1 hit.

PROSITE

PS00056. RIBOSOMAL_S17. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0CX47.

NextBio

969066.

Entry information

Entry name

RS11A_YEAST

Accession

Primary (citable) accession number: P0CX47
Secondary accession number(s): D6VQ48, O11852, P26781

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program