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Protein

60S ribosomal protein L2-B

Gene

RPL2B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 4050 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for uL2 in yeast.Curated

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • rRNA binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31294-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L2-B1 Publication
Alternative name(s):
L5
Large ribosomal subunit protein uL2-B1 Publication
RP8
YL6
Gene namesi
Name:RPL2B1 Publication
Synonyms:RPL5A
Ordered Locus Names:YIL018W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

SGDiS000001280 RPL2B

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00004097692 – 25460S ribosomal protein L2-BAdd BLAST253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei52PhosphoserineCombined sources1
Cross-linki93Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei95PhosphoserineCombined sources1
Cross-linki119Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki145Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei159PhosphoserineCombined sources1
Modified residuei160PhosphoserineCombined sources1
Modified residuei249PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP0CX46

PTM databases

CarbonylDBiP0CX46
iPTMnetiP0CX46

Expressioni

Gene expression databases

ExpressionAtlasiP0CX46 differential

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi31187, 332 interactors
34970, 50 interactors
IntActiP0CX46, 2 interactors
MINTiP0CX46

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V8Telectron microscopy8.10A1-254[»]
4V8Yelectron microscopy4.30BA2-254[»]
4V8Zelectron microscopy6.60BA2-254[»]
ProteinModelPortaliP0CX46
SMRiP0CX46
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000009907
InParanoidiP0CX46
KOiK02938
OrthoDBiEOG092C4DNU

Family and domain databases

Gene3Di2.30.30.30, 1 hit
4.10.950.10, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR022666 Rbsml_prot_L2_RNA-bd_dom
IPR014722 Rib_L2_dom2
IPR002171 Ribosomal_L2
IPR022669 Ribosomal_L2_C
IPR022671 Ribosomal_L2_CS
IPR014726 Ribosomal_L2_dom3
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR13691 PTHR13691, 1 hit
PfamiView protein in Pfam
PF00181 Ribosomal_L2, 1 hit
PF03947 Ribosomal_L2_C, 1 hit
PIRSFiPIRSF002158 Ribosomal_L2, 1 hit
SMARTiView protein in SMART
SM01383 Ribosomal_L2, 1 hit
SM01382 Ribosomal_L2_C, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
SSF50249 SSF50249, 1 hit
PROSITEiView protein in PROSITE
PS00467 RIBOSOMAL_L2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH
60 70 80 90 100
DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIYA GKKASLNVGN
110 120 130 140 150
VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL
160 170 180 190 200
PSGAKKVISS DARGVIGVIA GGGRVDKPLL KAGRAFHKYR LKRNSWPKTR
210 220 230 240 250
GVAMNPVDHP HGGGNHQHIG KASTISRGAV SGQKAGLIAA RRTGLLRGSQ

KTQD
Length:254
Mass (Da):27,408
Last modified:June 28, 2011 - v1
Checksum:i5159A980574DF05A
GO

Mass spectrometryi

Molecular mass is 27277.347 Da from positions 2 - 254. Determined by ESI. Average mass.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17360 mRNA Translation: AAA92284.1
Z46881 Genomic DNA Translation: CAA86974.1
BK006942 Genomic DNA Translation: DAA08527.1
PIRiS50243
RefSeqiNP_012246.1, NM_001179368.1
NP_116688.3, NM_001180030.3

Genome annotation databases

EnsemblFungiiYFR031C-A; YFR031C-A; YFR031C-A
YIL018W; YIL018W; YIL018W
GeneIDi850590
854794
KEGGisce:YFR031C-A
sce:YIL018W

Similar proteinsi

Entry informationi

Entry nameiRL2B_YEAST
AccessioniPrimary (citable) accession number: P0CX46
Secondary accession number(s): D6VTR2, P05736
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 25, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health