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P0CX45

- RL2A_YEAST

UniProt

P0CX45 - RL2A_YEAST

Protein

60S ribosomal protein L2-A

Gene

RPL2A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 31 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. rRNA binding Source: UniProtKB-KW
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30501-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L2-A
    Alternative name(s):
    L5
    RP8
    YL6
    Gene namesi
    Name:RPL2A
    Synonyms:RPL5B
    Ordered Locus Names:YFR031C-A
    ORF Names:YFR031BC
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFR031c-a.
    SGDiS000002104. RPL2A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 25425360S ribosomal protein L2-APRO_0000129761Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei52 – 521Phosphoserine1 Publication
    Modified residuei95 – 951Phosphoserine1 Publication
    Modified residuei159 – 1591Phosphoserine1 Publication
    Modified residuei160 – 1601Phosphoserine1 Publication
    Modified residuei249 – 2491Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP0CX45.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi31187. 162 interactions.
    34970. 104 interactions.
    IntActiP0CX45. 1 interaction.
    MINTiMINT-8285609.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Ymodel-B2-245[»]
    1S1Ielectron microscopy11.70B2-254[»]
    1VW8electron microscopy6.10B1-254[»]
    1VWUelectron microscopy6.10B1-254[»]
    3IZSelectron microscopy-B1-254[»]
    3J65electron microscopy8.70B1-254[»]
    3O58X-ray4.00B1-254[»]
    3O5HX-ray4.00B1-254[»]
    3U5EX-ray3.00A1-254[»]
    3U5IX-ray3.00A1-254[»]
    4CUWelectron microscopy3.70A1-254[»]
    ProteinModelPortaliP0CX45.
    SMRiP0CX45. Positions 2-253.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0CX45.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L2P family.Curated

    Phylogenomic databases

    KOiK02938.
    OrthoDBiEOG7GJ6QS.

    Family and domain databases

    Gene3Di2.30.30.30. 1 hit.
    2.40.50.140. 1 hit.
    4.10.950.10. 1 hit.
    InterProiIPR012340. NA-bd_OB-fold.
    IPR022666. Rbsml_prot_L2_RNA-bd_dom.
    IPR014722. Rib_L2_dom2.
    IPR002171. Ribosomal_L2.
    IPR022669. Ribosomal_L2_C.
    IPR022671. Ribosomal_L2_CS.
    IPR014726. Ribosomal_L2_dom3.
    IPR008991. Translation_prot_SH3-like.
    [Graphical view]
    PANTHERiPTHR13691. PTHR13691. 1 hit.
    PfamiPF00181. Ribosomal_L2. 1 hit.
    PF03947. Ribosomal_L2_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002158. Ribosomal_L2. 1 hit.
    SUPFAMiSSF50104. SSF50104. 1 hit.
    SSF50249. SSF50249. 1 hit.
    PROSITEiPS00467. RIBOSOMAL_L2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CX45-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH    50
    DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIYA GKKASLNVGN 100
    VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL 150
    PSGAKKVISS DARGVIGVIA GGGRVDKPLL KAGRAFHKYR LKRNSWPKTR 200
    GVAMNPVDHP HGGGNHQHIG KASTISRGAV SGQKAGLIAA RRTGLLRGSQ 250
    KTQD 254
    Length:254
    Mass (Da):27,408
    Last modified:June 28, 2011 - v1
    Checksum:i5159A980574DF05A
    GO

    Mass spectrometryi

    Molecular mass is 27277.347 Da from positions 2 - 254. Determined by ESI. Average mass.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17359 Genomic DNA. Translation: AAA92283.1.
    D50617 Genomic DNA. No translation available.
    U05820 Genomic DNA. Translation: AAA17418.1.
    BK006940 Genomic DNA. Translation: DAA12472.1.
    PIRiS50243.
    RefSeqiNP_012246.1. NM_001179368.1.
    NP_116688.3. NM_001180030.3.

    Genome annotation databases

    EnsemblFungiiYFR031C-A; YFR031C-A; YFR031C-A.
    YIL018W; YIL018W; YIL018W.
    GeneIDi850590.
    854794.
    KEGGisce:YFR031C-A.
    sce:YIL018W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17359 Genomic DNA. Translation: AAA92283.1 .
    D50617 Genomic DNA. No translation available.
    U05820 Genomic DNA. Translation: AAA17418.1 .
    BK006940 Genomic DNA. Translation: DAA12472.1 .
    PIRi S50243.
    RefSeqi NP_012246.1. NM_001179368.1.
    NP_116688.3. NM_001180030.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5Y model - B 2-245 [» ]
    1S1I electron microscopy 11.70 B 2-254 [» ]
    1VW8 electron microscopy 6.10 B 1-254 [» ]
    1VWU electron microscopy 6.10 B 1-254 [» ]
    3IZS electron microscopy - B 1-254 [» ]
    3J65 electron microscopy 8.70 B 1-254 [» ]
    3O58 X-ray 4.00 B 1-254 [» ]
    3O5H X-ray 4.00 B 1-254 [» ]
    3U5E X-ray 3.00 A 1-254 [» ]
    3U5I X-ray 3.00 A 1-254 [» ]
    4CUW electron microscopy 3.70 A 1-254 [» ]
    ProteinModelPortali P0CX45.
    SMRi P0CX45. Positions 2-253.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31187. 162 interactions.
    34970. 104 interactions.
    IntActi P0CX45. 1 interaction.
    MINTi MINT-8285609.

    Proteomic databases

    MaxQBi P0CX45.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR031C-A ; YFR031C-A ; YFR031C-A .
    YIL018W ; YIL018W ; YIL018W .
    GeneIDi 850590.
    854794.
    KEGGi sce:YFR031C-A.
    sce:YIL018W.

    Organism-specific databases

    CYGDi YFR031c-a.
    SGDi S000002104. RPL2A.

    Phylogenomic databases

    KOi K02938.
    OrthoDBi EOG7GJ6QS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30501-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P0CX45.
    NextBioi 966437.

    Family and domain databases

    Gene3Di 2.30.30.30. 1 hit.
    2.40.50.140. 1 hit.
    4.10.950.10. 1 hit.
    InterProi IPR012340. NA-bd_OB-fold.
    IPR022666. Rbsml_prot_L2_RNA-bd_dom.
    IPR014722. Rib_L2_dom2.
    IPR002171. Ribosomal_L2.
    IPR022669. Ribosomal_L2_C.
    IPR022671. Ribosomal_L2_CS.
    IPR014726. Ribosomal_L2_dom3.
    IPR008991. Translation_prot_SH3-like.
    [Graphical view ]
    PANTHERi PTHR13691. PTHR13691. 1 hit.
    Pfami PF00181. Ribosomal_L2. 1 hit.
    PF03947. Ribosomal_L2_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002158. Ribosomal_L2. 1 hit.
    SUPFAMi SSF50104. SSF50104. 1 hit.
    SSF50249. SSF50249. 1 hit.
    PROSITEi PS00467. RIBOSOMAL_L2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterisation of Saccharomyces cerevisiae genes encoding ribosomal protein YL6."
      Moore J., Jacobs H.T., Kaiser K.
      Mol. Gen. Genet. 247:247-254(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204660 / DBY746.
    2. "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
      Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
      Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
      Otaka E., Higo K., Itoh T.
      Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE OF 2-41, CLEAVAGE OF INITIATOR METHIONINE.
    6. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
      Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
      J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-21.
    7. "SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family."
      Strunnikov A.V., Hogan E., Koshland D.
      Genes Dev. 9:587-599(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 226-254.
    8. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    9. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
      Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
      Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY.
    10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    11. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; SER-159; SER-160 AND SER-249, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 2-245, ELECTRON MICROSCOPY.
    15. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
    16. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
    17. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

    Entry informationi

    Entry nameiRL2A_YEAST
    AccessioniPrimary (citable) accession number: P0CX45
    Secondary accession number(s): D6VTR2, P05736
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 31 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 2 genes for L2 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3