60S ribosomal protein L2-A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P0CX45 (RL2A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 17. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S ribosomal protein L2-A

Alternative name(s):
L5
RP8
YL6

Gene names

Name:	RPL2A
Synonyms:	RPL5B
Ordered Locus Names:	YFR031C-A
ORF Names:	YFR031BC

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.8
Subcellular location	Cytoplasm Ref.10.
Miscellaneous	There are 2 genes for L2 in yeast.
Sequence similarities	Belongs to the ribosomal protein L2P family.
Mass spectrometry	Molecular mass is 27277.347 Da from positions 2 - 254. Determined by ESI. Average mass. Ref.9

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred from direct assay PubMed 18782943. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Inferred from direct assay PubMed 18782943. Source: SGD
Molecular_function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from direct assay PubMed 18782943. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5 Ref.6

Chain

2 – 254

253

60S ribosomal protein L2-A

PRO_0000129761

Amino acid modifications

Modified residue

Phosphothreonine Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphothreonine Ref.11

Modified residue

159

Phosphoserine Ref.11

Modified residue

160

Phosphoserine Ref.11

Modified residue

243

Phosphothreonine Ref.11

Modified residue

249

Phosphoserine Ref.11

Sequences

Sequence

Length

Mass (Da)

Tools

P0CX45 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 5159A980574DF05A
Show »

FASTA

254

27,408

        10         20         30         40         50         60 
MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH DSGRGAPLAK 

        70         80         90        100        110        120 
VVFRDPYKYR LREEIFIANE GVHTGQFIYA GKKASLNVGN VLPLGSVPEG TIVSNVEEKP 

       130        140        150        160        170        180 
GDRGALARAS GNYVIIIGHN PDENKTRVRL PSGAKKVISS DARGVIGVIA GGGRVDKPLL 

       190        200        210        220        230        240 
KAGRAFHKYR LKRNSWPKTR GVAMNPVDHP HGGGNHQHIG KASTISRGAV SGQKAGLIAA 

       250 
RRTGLLRGSQ KTQD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterisation of Saccharomyces cerevisiae genes encoding ribosomal protein YL6." Moore J., Jacobs H.T., Kaiser K. Mol. Gen. Genet. 247:247-254(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204660 / DBY746.
[2]	"Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y. Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes." Otaka E., Higo K., Itoh T. Mol. Gen. Genet. 195:544-546(1984) Cited for: PROTEIN SEQUENCE OF 2-41.
[6]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21.
[7]	"SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family." Strunnikov A.V., Hogan E., Koshland D. Genes Dev. 9:587-599(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 226-254.
[8]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[9]	"Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR." Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D. Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20; SER-52; THR-84; SER-159; SER-160; THR-243 AND SER-249, MASS SPECTROMETRY.
[12]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 2-245, ELECTRON MICROSCOPY.
[13]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U17359 Genomic DNA. Translation: AAA92283.1.
D50617 Genomic DNA. No translation available.
U05820 Genomic DNA. Translation: AAA17418.1.
BK006940 Genomic DNA. Translation: DAA12472.1.

PIR

S50243.

RefSeq

NP_012246.1. NM_001179368.1.
NP_116688.3. NM_001180030.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	B	2-245	[»]
1S1I	electron microscopy	11.70	B	2-254	[»]
3IZS	electron microscopy	-	B	1-254	[»]
3O58	X-ray	4.00	B	1-254	[»]
3O5H	X-ray	4.00	B	1-254	[»]
3U5E	X-ray	3.00	A	1-254	[»]
3U5I	X-ray	3.00	A	1-254	[»]

ProteinModelPortal

P0CX45.

SMR

P0CX45. Positions 2-253.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YFR031C-A; YFR031C-A; YFR031C-A.
YIL018W; YIL018W; YIL018W.

GeneID

850590.
854794.

KEGG

sce:YFR031C-A.
sce:YFR032C-A.
sce:YIL018W.

Organism-specific databases

CYGD

YFR031c-a.

SGD

S000002104. RPL2A.

Phylogenomic databases

K02905.
K02938.

Gene expression databases

ArrayExpress

P0CX45.

GermOnline

YFR031C-A. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.

InterPro

IPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]

PANTHER

PTHR13691. PTHR13691. 1 hit.

Pfam

PF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]

PIRSF

PIRSF002158. Ribosomal_L2. 1 hit.

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.
SSF50104. Transl_SH3_like. 1 hit.

PROSITE

PS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0CX45.

NextBio

966437.

Entry information

Entry name

RL2A_YEAST

Accession

Primary (citable) accession number: P0CX45
Secondary accession number(s): D6VTR2, P05736

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program