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Protein

60S ribosomal protein L2-A

Gene

RPL2A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. rRNA binding Source: UniProtKB-KW
  2. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30501-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L2-A
Alternative name(s):
L5
RP8
YL6
Gene namesi
Name:RPL2A
Synonyms:RPL5B
Ordered Locus Names:YFR031C-A
ORF Names:YFR031BC
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFR031c-a.
SGDiS000002104. RPL2A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 25425360S ribosomal protein L2-APRO_0000129761Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521Phosphoserine1 Publication
Modified residuei95 – 951Phosphoserine1 Publication
Modified residuei159 – 1591Phosphoserine1 Publication
Modified residuei160 – 1601Phosphoserine1 Publication
Modified residuei249 – 2491Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CX45.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX45. differential.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi31187. 163 interactions.
34970. 105 interactions.
IntActiP0CX45. 1 interaction.
MINTiMINT-8285609.

Structurei

Secondary structure

1
254
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 94Combined sources
Helixi10 – 123Combined sources
Helixi14 – 163Combined sources
Helixi20 – 223Combined sources
Helixi34 – 385Combined sources
Beta strandi41 – 499Combined sources
Beta strandi58 – 647Combined sources
Beta strandi68 – 7710Combined sources
Beta strandi87 – 915Combined sources
Beta strandi101 – 1033Combined sources
Helixi104 – 1063Combined sources
Beta strandi112 – 1165Combined sources
Beta strandi118 – 1225Combined sources
Beta strandi134 – 1407Combined sources
Turni141 – 1444Combined sources
Beta strandi145 – 1495Combined sources
Beta strandi151 – 1533Combined sources
Beta strandi155 – 1595Combined sources
Beta strandi163 – 1675Combined sources
Beta strandi169 – 1713Combined sources
Helixi174 – 1763Combined sources
Helixi182 – 1909Combined sources
Beta strandi191 – 1944Combined sources
Helixi201 – 2033Combined sources
Helixi206 – 2083Combined sources
Turni210 – 2123Combined sources
Turni214 – 2174Combined sources
Beta strandi223 – 2253Combined sources
Turni231 – 2333Combined sources
Beta strandi236 – 2383Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-B2-245[»]
3J6Xelectron microscopy6.10B1-254[»]
3J6Yelectron microscopy6.10B1-254[»]
3J77electron microscopy6.20B1-254[»]
3J78electron microscopy6.30B1-254[»]
4U3MX-ray3.00D/L2-254[»]
4U3NX-ray3.20D/L2-254[»]
4U3UX-ray2.90D/L2-254[»]
4U4NX-ray3.10D/L2-254[»]
4U4OX-ray3.60D/L2-254[»]
4U4QX-ray3.00D/L2-254[»]
4U4RX-ray2.80D/L2-254[»]
4U4UX-ray3.00D/L2-254[»]
4U4YX-ray3.20D/L2-254[»]
4U4ZX-ray3.10D/L2-254[»]
4U50X-ray3.20D/L2-254[»]
4U51X-ray3.20D/L2-254[»]
4U52X-ray3.00D/L2-254[»]
4U53X-ray3.30D/L2-254[»]
4U55X-ray3.20D/L2-254[»]
4U56X-ray3.45D/L2-254[»]
4U6FX-ray3.10D/L2-254[»]
4V4Belectron microscopy11.70B2-254[»]
4V6Ielectron microscopy-B1-254[»]
4V7Felectron microscopy8.70B1-254[»]
4V7RX-ray4.00B1-254[»]
4V88X-ray3.00A1-254[»]
4V91electron microscopy3.70A1-254[»]
ProteinModelPortaliP0CX45.
SMRiP0CX45. Positions 2-253.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX45.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L2P family.Curated

Phylogenomic databases

InParanoidiP0CX45.
KOiK02938.
OrthoDBiEOG7GJ6QS.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR13691. PTHR13691. 1 hit.
PfamiPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002158. Ribosomal_L2. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
PROSITEiPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX45-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH
60 70 80 90 100
DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIYA GKKASLNVGN
110 120 130 140 150
VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL
160 170 180 190 200
PSGAKKVISS DARGVIGVIA GGGRVDKPLL KAGRAFHKYR LKRNSWPKTR
210 220 230 240 250
GVAMNPVDHP HGGGNHQHIG KASTISRGAV SGQKAGLIAA RRTGLLRGSQ

KTQD
Length:254
Mass (Da):27,408
Last modified:June 28, 2011 - v1
Checksum:i5159A980574DF05A
GO

Mass spectrometryi

Molecular mass is 27277.347 Da from positions 2 - 254. Determined by ESI. Average mass.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17359 Genomic DNA. Translation: AAA92283.1.
D50617 Genomic DNA. No translation available.
U05820 Genomic DNA. Translation: AAA17418.1.
BK006940 Genomic DNA. Translation: DAA12472.1.
PIRiS50243.
RefSeqiNP_012246.1. NM_001179368.1.
NP_116688.3. NM_001180030.3.

Genome annotation databases

EnsemblFungiiYFR031C-A; YFR031C-A; YFR031C-A.
YIL018W; YIL018W; YIL018W.
GeneIDi850590.
854794.
KEGGisce:YFR031C-A.
sce:YIL018W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17359 Genomic DNA. Translation: AAA92283.1.
D50617 Genomic DNA. No translation available.
U05820 Genomic DNA. Translation: AAA17418.1.
BK006940 Genomic DNA. Translation: DAA12472.1.
PIRiS50243.
RefSeqiNP_012246.1. NM_001179368.1.
NP_116688.3. NM_001180030.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-B2-245[»]
3J6Xelectron microscopy6.10B1-254[»]
3J6Yelectron microscopy6.10B1-254[»]
3J77electron microscopy6.20B1-254[»]
3J78electron microscopy6.30B1-254[»]
4U3MX-ray3.00D/L2-254[»]
4U3NX-ray3.20D/L2-254[»]
4U3UX-ray2.90D/L2-254[»]
4U4NX-ray3.10D/L2-254[»]
4U4OX-ray3.60D/L2-254[»]
4U4QX-ray3.00D/L2-254[»]
4U4RX-ray2.80D/L2-254[»]
4U4UX-ray3.00D/L2-254[»]
4U4YX-ray3.20D/L2-254[»]
4U4ZX-ray3.10D/L2-254[»]
4U50X-ray3.20D/L2-254[»]
4U51X-ray3.20D/L2-254[»]
4U52X-ray3.00D/L2-254[»]
4U53X-ray3.30D/L2-254[»]
4U55X-ray3.20D/L2-254[»]
4U56X-ray3.45D/L2-254[»]
4U6FX-ray3.10D/L2-254[»]
4V4Belectron microscopy11.70B2-254[»]
4V6Ielectron microscopy-B1-254[»]
4V7Felectron microscopy8.70B1-254[»]
4V7RX-ray4.00B1-254[»]
4V88X-ray3.00A1-254[»]
4V91electron microscopy3.70A1-254[»]
ProteinModelPortaliP0CX45.
SMRiP0CX45. Positions 2-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31187. 163 interactions.
34970. 105 interactions.
IntActiP0CX45. 1 interaction.
MINTiMINT-8285609.

Proteomic databases

MaxQBiP0CX45.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYFR031C-A; YFR031C-A; YFR031C-A.
YIL018W; YIL018W; YIL018W.
GeneIDi850590.
854794.
KEGGisce:YFR031C-A.
sce:YIL018W.

Organism-specific databases

CYGDiYFR031c-a.
SGDiS000002104. RPL2A.

Phylogenomic databases

InParanoidiP0CX45.
KOiK02938.
OrthoDBiEOG7GJ6QS.

Enzyme and pathway databases

BioCyciYEAST:G3O-30501-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

EvolutionaryTraceiP0CX45.
NextBioi966437.

Gene expression databases

ExpressionAtlasiP0CX45. differential.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR13691. PTHR13691. 1 hit.
PfamiPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002158. Ribosomal_L2. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
PROSITEiPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterisation of Saccharomyces cerevisiae genes encoding ribosomal protein YL6."
    Moore J., Jacobs H.T., Kaiser K.
    Mol. Gen. Genet. 247:247-254(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204660 / DBY746.
  2. "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
    Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
    Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
    Otaka E., Higo K., Itoh T.
    Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE OF 2-41, CLEAVAGE OF INITIATOR METHIONINE.
  6. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
    Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
    J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21.
  7. "SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family."
    Strunnikov A.V., Hogan E., Koshland D.
    Genes Dev. 9:587-599(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 226-254.
  8. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  9. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
    Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
    Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; SER-159; SER-160 AND SER-249, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 2-245, ELECTRON MICROSCOPY.
  15. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
  16. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  17. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL2A_YEAST
AccessioniPrimary (citable) accession number: P0CX45
Secondary accession number(s): D6VTR2, P05736
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: February 4, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 2 genes for L2 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.