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Protein

60S ribosomal protein L1-A

Gene

RPL1A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. uL1 forms part of the L1 stalk, a mobile element that plays a role in evacuating the exit-site tRNA.1 Publication

Miscellaneous

Present with 116000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for uL1 in yeast.Curated

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of LSU-rRNA Source: GO_Central
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-34109-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L1-A1 Publication
Alternative name(s):
L10a
Large ribosomal subunit protein uL1-A1 Publication
Gene namesi
Name:RPL1A1 Publication
Synonyms:SSM1, SSM1A
Ordered Locus Names:YPL220W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

SGDiS000006141. RPL1A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001258422 – 21760S ribosomal protein L1-AAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei47N6-methyllysine; by RKM51 Publication1
Modified residuei79PhosphoserineCombined sources1
Modified residuei86PhosphoserineCombined sources1

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiP0CX43.
PRIDEiP0CX43.
TopDownProteomicsiP0CX43.

PTM databases

iPTMnetiP0CX43.

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). uL1 forms part of the L1 stalk (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi33117. 171 interactors.
35965. 132 interactors.
IntActiP0CX43. 3 interactors.
MINTiMINT-495020.
STRINGi4932.YPL220W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-A4-216[»]
2NOQelectron microscopy7.30G4-216[»]
3J0Pelectron microscopy10.60B4-216[»]
3J0Qelectron microscopy10.60B4-216[»]
3J6Xelectron microscopy6.10L11-217[»]
3J6Yelectron microscopy6.10L11-217[»]
3J77electron microscopy6.20L11-217[»]
3J78electron microscopy6.30L11-217[»]
4V4Belectron microscopy11.70BA1-217[»]
4V6Ielectron microscopy8.80BA1-217[»]
4V7Felectron microscopy8.70A1-217[»]
4V7Helectron microscopy8.90A4-216[»]
4V7RX-ray4.00BA/DA1-217[»]
4V91electron microscopy3.70t1-217[»]
5JCSelectron microscopy9.50I1-217[»]
5JUOelectron microscopy4.00E1-217[»]
5JUPelectron microscopy3.50E1-217[»]
5JUSelectron microscopy4.20E1-217[»]
5JUTelectron microscopy4.00E1-217[»]
5JUUelectron microscopy4.00E1-217[»]
ProteinModelPortaliP0CX43.
SMRiP0CX43.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX43.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP0CX43.
KOiK02865.
OMAiIHLAINF.
OrthoDBiEOG092C4PD0.

Family and domain databases

CDDicd00403. Ribosomal_L1. 1 hit.
InterProiView protein in InterPro
IPR002143. Ribosomal_L1.
IPR023674. Ribosomal_L1-like.
IPR028364. Ribosomal_L1/biogenesis.
IPR023673. Ribosomal_L1_CS.
PfamiView protein in Pfam
PF00687. Ribosomal_L1. 1 hit.
PIRSFiPIRSF002155. Ribosomal_L1. 1 hit.
SUPFAMiSSF56808. SSF56808. 1 hit.
PROSITEiView protein in PROSITE
PS01199. RIBOSOMAL_L1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS
60 70 80 90 100
GSLKLPNCPR PNMSICIFGD AFDVDRAKSC GVDAMSVDDL KKLNKNKKLI
110 120 130 140 150
KKLSKKYNAF IASEVLIKQV PRLLGPQLSK AGKFPTPVSH NDDLYGKVTD
160 170 180 190 200
VRSTIKFQLK KVLCLAVAVG NVEMEEDVLV NQILMSVNFF VSLLKKNWQN
210
VGSLVVKSSM GPAFRLY
Length:217
Mass (Da):24,486
Last modified:June 28, 2011 - v1
Checksum:iC890A90505655F2A
GO

Mass spectrometryi

Molecular mass is 24410.862 Da from positions 2 - 217. Determined by ESI. Average mass with 1 acetylation and 1 methylation modification.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70985 Genomic DNA. Translation: CAA50314.1.
Z73576 Genomic DNA. Translation: CAA97935.1.
BK006949 Genomic DNA. Translation: DAA11216.1.
PIRiS53893.
RefSeqiNP_011380.3. NM_001181000.3.
NP_015104.1. NM_001184034.1.

Genome annotation databases

EnsemblFungiiYGL135W; YGL135W; YGL135W.
YPL220W; YPL220W; YPL220W.
GeneIDi852742.
855881.
KEGGisce:YGL135W.
sce:YPL220W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRL1A_YEAST
AccessioniPrimary (citable) accession number: P0CX43
Secondary accession number(s): D6VU14, P53030
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: July 5, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names