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P0CX43

- RL1A_YEAST

UniProt

P0CX43 - RL1A_YEAST

Protein

60S ribosomal protein L1-A

Gene

RPL1A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 30 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. RNA binding Source: InterPro
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34109-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L1-A
    Alternative name(s):
    L10a
    Gene namesi
    Name:RPL1A
    Synonyms:SSM1, SSM1A
    Ordered Locus Names:YPL220W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL220w.
    SGDiS000006141. RPL1A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 21721660S ribosomal protein L1-APRO_0000125842Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications
    Modified residuei47 – 471N6-methyllysine; by RKM51 Publication
    Modified residuei79 – 791Phosphoserine2 Publications
    Modified residuei86 – 861Phosphoserine1 Publication

    Post-translational modificationi

    N-terminally acetylated by acetyltransferase NatA.2 Publications

    Keywords - PTMi

    Acetylation, Methylation, Phosphoprotein

    Proteomic databases

    MaxQBiP0CX43.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi33117. 143 interactions.
    35965. 119 interactions.
    IntActiP0CX43. 1 interaction.
    MINTiMINT-495020.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Ymodel-A4-216[»]
    1S1Ielectron microscopy11.70A1-217[»]
    1VW8electron microscopy6.10A1-217[»]
    1VWUelectron microscopy6.10A1-217[»]
    2NOQelectron microscopy7.30G4-216[»]
    3IZSelectron microscopy-A1-217[»]
    3J0Pelectron microscopy10.60B4-216[»]
    3J0Qelectron microscopy10.60B4-216[»]
    3J65electron microscopy8.70A1-217[»]
    3JYWelectron microscopy8.90A4-216[»]
    3O58X-ray4.00A1-217[»]
    3O5HX-ray4.00A1-217[»]
    4CUWelectron microscopy3.70t1-217[»]
    ProteinModelPortaliP0CX43.
    SMRiP0CX43. Positions 4-216.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0CX43.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L1P family.Curated

    Phylogenomic databases

    KOiK02865.
    OrthoDBiEOG73Z358.

    Family and domain databases

    Gene3Di3.30.190.20. 2 hits.
    3.40.50.790. 1 hit.
    InterProiIPR002143. Ribosomal_L1.
    IPR023674. Ribosomal_L1-like.
    IPR028364. Ribosomal_L1/biogenesis.
    IPR016094. Ribosomal_L1_2-a/b-sand.
    IPR016095. Ribosomal_L1_3-a/b-sand.
    IPR023673. Ribosomal_L1_CS.
    [Graphical view]
    PfamiPF00687. Ribosomal_L1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002155. Ribosomal_L1. 1 hit.
    SUPFAMiSSF56808. SSF56808. 1 hit.
    PROSITEiPS01199. RIBOSOMAL_L1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CX43-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS    50
    GSLKLPNCPR PNMSICIFGD AFDVDRAKSC GVDAMSVDDL KKLNKNKKLI 100
    KKLSKKYNAF IASEVLIKQV PRLLGPQLSK AGKFPTPVSH NDDLYGKVTD 150
    VRSTIKFQLK KVLCLAVAVG NVEMEEDVLV NQILMSVNFF VSLLKKNWQN 200
    VGSLVVKSSM GPAFRLY 217
    Length:217
    Mass (Da):24,486
    Last modified:June 28, 2011 - v1
    Checksum:iC890A90505655F2A
    GO

    Mass spectrometryi

    Molecular mass is 24410.862 Da from positions 2 - 217. Determined by ESI. Average mass with 1 acetylation and 1 methylation modification.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70985 Genomic DNA. Translation: CAA50314.1.
    Z73576 Genomic DNA. Translation: CAA97935.1.
    BK006949 Genomic DNA. Translation: DAA11216.1.
    PIRiS53893.
    RefSeqiNP_011380.3. NM_001181000.3.
    NP_015104.1. NM_001184034.1.

    Genome annotation databases

    EnsemblFungiiYGL135W; YGL135W; YGL135W.
    YPL220W; YPL220W; YPL220W.
    GeneIDi852742.
    855881.
    KEGGisce:YGL135W.
    sce:YPL220W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70985 Genomic DNA. Translation: CAA50314.1 .
    Z73576 Genomic DNA. Translation: CAA97935.1 .
    BK006949 Genomic DNA. Translation: DAA11216.1 .
    PIRi S53893.
    RefSeqi NP_011380.3. NM_001181000.3.
    NP_015104.1. NM_001184034.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5Y model - A 4-216 [» ]
    1S1I electron microscopy 11.70 A 1-217 [» ]
    1VW8 electron microscopy 6.10 A 1-217 [» ]
    1VWU electron microscopy 6.10 A 1-217 [» ]
    2NOQ electron microscopy 7.30 G 4-216 [» ]
    3IZS electron microscopy - A 1-217 [» ]
    3J0P electron microscopy 10.60 B 4-216 [» ]
    3J0Q electron microscopy 10.60 B 4-216 [» ]
    3J65 electron microscopy 8.70 A 1-217 [» ]
    3JYW electron microscopy 8.90 A 4-216 [» ]
    3O58 X-ray 4.00 A 1-217 [» ]
    3O5H X-ray 4.00 A 1-217 [» ]
    4CUW electron microscopy 3.70 t 1-217 [» ]
    ProteinModelPortali P0CX43.
    SMRi P0CX43. Positions 4-216.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33117. 143 interactions.
    35965. 119 interactions.
    IntActi P0CX43. 1 interaction.
    MINTi MINT-495020.

    Proteomic databases

    MaxQBi P0CX43.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL135W ; YGL135W ; YGL135W .
    YPL220W ; YPL220W ; YPL220W .
    GeneIDi 852742.
    855881.
    KEGGi sce:YGL135W.
    sce:YPL220W.

    Organism-specific databases

    CYGDi YPL220w.
    SGDi S000006141. RPL1A.

    Phylogenomic databases

    KOi K02865.
    OrthoDBi EOG73Z358.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34109-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P0CX43.
    NextBioi 972159.

    Family and domain databases

    Gene3Di 3.30.190.20. 2 hits.
    3.40.50.790. 1 hit.
    InterProi IPR002143. Ribosomal_L1.
    IPR023674. Ribosomal_L1-like.
    IPR028364. Ribosomal_L1/biogenesis.
    IPR016094. Ribosomal_L1_2-a/b-sand.
    IPR016095. Ribosomal_L1_3-a/b-sand.
    IPR023673. Ribosomal_L1_CS.
    [Graphical view ]
    Pfami PF00687. Ribosomal_L1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002155. Ribosomal_L1. 1 hit.
    SUPFAMi SSF56808. SSF56808. 1 hit.
    PROSITEi PS01199. RIBOSOMAL_L1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The duplicated Saccharomyces cerevisiae gene SSM1 encodes a eucaryotic homolog of the eubacterial and archaebacterial L1 ribosomal proteins."
      Petitjean A., Bonneaud N., Lacroute F.
      Mol. Cell. Biol. 15:5071-5081(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    5. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
    6. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
      Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
      Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79 AND SER-86, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "The ribosomal L1 protuberance in yeast is methylated on a lysine residue catalyzed by a seven beta-strand methyltransferase."
      Webb K.J., Al-Hadid Q., Zurita-Lopez C.I., Young B.D., Lipson R.S., Clarke S.G.
      J. Biol. Chem. 286:18405-18413(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT LYS-47, IDENTIFICATION BY MASS SPECTROMETRY.
    13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 4-216, ELECTRON MICROSCOPY.
    15. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
    16. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.3 ANGSTROMS) OF 4-216.

    Entry informationi

    Entry nameiRL1A_YEAST
    AccessioniPrimary (citable) accession number: P0CX43
    Secondary accession number(s): D6VU14, P53030
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 116000 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for L1 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3