P0CX43 (RL1A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L1-A

Alternative name(s):
L10a

Gene names

Name:	RPL1A
Synonyms:	SSM1, SSM1A
Ordered Locus Names:	YPL220W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm Ref.7.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Miscellaneous	Present with 116000 molecules/cell in log phase SD medium. There are 2 genes for L1 in yeast.
Sequence similarities	Belongs to the ribosomal protein L1P family.
Mass spectrometry	Molecular mass is 24410.862 Da from positions 2 - 217. Determined by ESI. Average mass with 1 acetylation and 1 methylation modification. Ref.6

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Methylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.4. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.4. Source: SGD
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro structural constituent of ribosome Traceable author statement Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 217	216	60S ribosomal protein L1-A		PRO_0000125842
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.5
	Modified residue	7	1	Phosphoserine Ref.10 Ref.11
	Modified residue	47	1	N6-methyllysine; by RKM5 Ref.12
	Modified residue	79	1	Phosphoserine Ref.9
	Modified residue	86	1	Phosphoserine Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P0CX43 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: C890A90505655F2A	FASTA	217	24,486
	10 20 30 40 50 60 MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS GSLKLPNCPR 70 80 90 100 110 120 PNMSICIFGD AFDVDRAKSC GVDAMSVDDL KKLNKNKKLI KKLSKKYNAF IASEVLIKQV 130 140 150 160 170 180 PRLLGPQLSK AGKFPTPVSH NDDLYGKVTD VRSTIKFQLK KVLCLAVAVG NVEMEEDVLV 190 200 210 NQILMSVNFF VSLLKKNWQN VGSLVVKSSM GPAFRLY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The duplicated Saccharomyces cerevisiae gene SSM1 encodes a eucaryotic homolog of the eubacterial and archaebacterial L1 ribosomal proteins." Petitjean A., Bonneaud N., Lacroute F. Mol. Cell. Biol. 15:5071-5081(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[6]	"Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR." Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D. Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7 AND SER-86, MASS SPECTROMETRY.
[12]	"The ribosomal L1 protuberance in yeast is methylated on a lysine residue catalyzed by a seven beta-strand methyltransferase." Webb K.J., Al-Hadid Q., Zurita-Lopez C.I., Young B.D., Lipson R.S., Clarke S.G. J. Biol. Chem. 286:18405-18413(2011) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT LYS-47, MASS SPECTROMETRY.
[13]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 4-216, ELECTRON MICROSCOPY.
[14]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
[15]	"Structure of the ribosome-bound cricket paralysis virus IRES RNA." Schueler M., Connell S.R., Lescoute A., Giesebrecht J., Dabrowski M., Schroeer B., Mielke T., Penczek P.A., Westhof E., Spahn C.M.T. Nat. Struct. Mol. Biol. 13:1092-1096(2006) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.3 ANGSTROMS) OF 4-216.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X70985 Genomic DNA. Translation: CAA50314.1.
Z73576 Genomic DNA. Translation: CAA97935.1.
BK006949 Genomic DNA. Translation: DAA11216.1.

PIR

S53893.

RefSeq

NP_011380.3. NM_001181000.3.
NP_015104.1. NM_001184034.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	A	4-216	[»]
1S1I	electron microscopy	11.70	A	1-217	[»]
2NOQ	electron microscopy	7.30	G	4-216	[»]
3IZS	electron microscopy	-	A	1-217	[»]
3O58	X-ray	4.00	A	1-217	[»]
3O5H	X-ray	4.00	A	1-217	[»]

ProteinModelPortal

P0CX43.

SMR

P0CX43. Positions 4-216.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL135W; YGL135W; YGL135W.
YPL220W; YPL220W; YPL220W.

GeneID

852742.
855881.

KEGG

sce:YGL130W.
sce:YGL135W.
sce:YPL220W.

Organism-specific databases

CYGD

YPL220w.

SGD

S000006141. RPL1A.

Phylogenomic databases

KO

K00987.
K02865.

Gene expression databases

ArrayExpress

P0CX43.

GermOnline

YPL220W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.30.190.20. 2 hits.
3.40.50.790. 1 hit.

InterPro

IPR002143. Ribosomal_L1.
IPR016094. Ribosomal_L1_2-a/b-sand.
IPR016095. Ribosomal_L1_3-a/b-sand.
IPR023673. Ribosomal_L1_CS.
IPR023674. Ribosomal_L1_SF.
[Graphical view]

Pfam

PF00687. Ribosomal_L1. 1 hit.
[Graphical view]

PIRSF

PIRSF002155. Ribosomal_L1. 1 hit.

SUPFAM

SSF56808. Ribosomal_L1. 1 hit.

PROSITE

PS01199. RIBOSOMAL_L1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0CX43.

NextBio

972159.

Entry information

Entry name

RL1A_YEAST

Accession

Primary (citable) accession number: P0CX43
Secondary accession number(s): D6VU14, P53030

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program