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P0CX43

- RL1A_YEAST

UniProt

P0CX43 - RL1A_YEAST

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Protein

60S ribosomal protein L1-A

Gene

RPL1A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-34109-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L1-A
Alternative name(s):
L10a
Gene namesi
Name:RPL1A
Synonyms:SSM1, SSM1A
Ordered Locus Names:YPL220W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL220w.
SGDiS000006141. RPL1A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 21721660S ribosomal protein L1-APRO_0000125842Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications
Modified residuei47 – 471N6-methyllysine; by RKM51 Publication
Modified residuei79 – 791Phosphoserine2 Publications
Modified residuei86 – 861Phosphoserine1 Publication

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.2 Publications

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiP0CX43.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi33117. 144 interactions.
35965. 120 interactions.
IntActiP0CX43. 1 interaction.
MINTiMINT-495020.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-A4-216[»]
1S1Ielectron microscopy11.70A1-217[»]
1VW8electron microscopy6.10A1-217[»]
1VWUelectron microscopy6.10A1-217[»]
1VXVelectron microscopy6.20A1-217[»]
1VXYelectron microscopy6.30A1-217[»]
2NOQelectron microscopy7.30G4-216[»]
3IZSelectron microscopy-A1-217[»]
3J0Pelectron microscopy10.60B4-216[»]
3J0Qelectron microscopy10.60B4-216[»]
3J65electron microscopy8.70A1-217[»]
3JYWelectron microscopy8.90A4-216[»]
3O58X-ray4.00A1-217[»]
3O5HX-ray4.00A1-217[»]
4CUWelectron microscopy3.70t1-217[»]
ProteinModelPortaliP0CX43.
SMRiP0CX43. Positions 1-217.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CX43.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L1P family.Curated

Phylogenomic databases

InParanoidiP0CX43.
KOiK02865.
OrthoDBiEOG73Z358.

Family and domain databases

Gene3Di3.30.190.20. 2 hits.
3.40.50.790. 1 hit.
InterProiIPR002143. Ribosomal_L1.
IPR023674. Ribosomal_L1-like.
IPR028364. Ribosomal_L1/biogenesis.
IPR016094. Ribosomal_L1_2-a/b-sand.
IPR016095. Ribosomal_L1_3-a/b-sand.
IPR023673. Ribosomal_L1_CS.
[Graphical view]
PfamiPF00687. Ribosomal_L1. 1 hit.
[Graphical view]
PIRSFiPIRSF002155. Ribosomal_L1. 1 hit.
SUPFAMiSSF56808. SSF56808. 1 hit.
PROSITEiPS01199. RIBOSOMAL_L1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX43-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS
60 70 80 90 100
GSLKLPNCPR PNMSICIFGD AFDVDRAKSC GVDAMSVDDL KKLNKNKKLI
110 120 130 140 150
KKLSKKYNAF IASEVLIKQV PRLLGPQLSK AGKFPTPVSH NDDLYGKVTD
160 170 180 190 200
VRSTIKFQLK KVLCLAVAVG NVEMEEDVLV NQILMSVNFF VSLLKKNWQN
210
VGSLVVKSSM GPAFRLY
Length:217
Mass (Da):24,486
Last modified:June 28, 2011 - v1
Checksum:iC890A90505655F2A
GO

Mass spectrometryi

Molecular mass is 24410.862 Da from positions 2 - 217. Determined by ESI. Average mass with 1 acetylation and 1 methylation modification.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70985 Genomic DNA. Translation: CAA50314.1.
Z73576 Genomic DNA. Translation: CAA97935.1.
BK006949 Genomic DNA. Translation: DAA11216.1.
PIRiS53893.
RefSeqiNP_011380.3. NM_001181000.3.
NP_015104.1. NM_001184034.1.

Genome annotation databases

EnsemblFungiiYGL135W; YGL135W; YGL135W.
YPL220W; YPL220W; YPL220W.
GeneIDi852742.
855881.
KEGGisce:YGL135W.
sce:YPL220W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70985 Genomic DNA. Translation: CAA50314.1 .
Z73576 Genomic DNA. Translation: CAA97935.1 .
BK006949 Genomic DNA. Translation: DAA11216.1 .
PIRi S53893.
RefSeqi NP_011380.3. NM_001181000.3.
NP_015104.1. NM_001184034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K5Y model - A 4-216 [» ]
1S1I electron microscopy 11.70 A 1-217 [» ]
1VW8 electron microscopy 6.10 A 1-217 [» ]
1VWU electron microscopy 6.10 A 1-217 [» ]
1VXV electron microscopy 6.20 A 1-217 [» ]
1VXY electron microscopy 6.30 A 1-217 [» ]
2NOQ electron microscopy 7.30 G 4-216 [» ]
3IZS electron microscopy - A 1-217 [» ]
3J0P electron microscopy 10.60 B 4-216 [» ]
3J0Q electron microscopy 10.60 B 4-216 [» ]
3J65 electron microscopy 8.70 A 1-217 [» ]
3JYW electron microscopy 8.90 A 4-216 [» ]
3O58 X-ray 4.00 A 1-217 [» ]
3O5H X-ray 4.00 A 1-217 [» ]
4CUW electron microscopy 3.70 t 1-217 [» ]
ProteinModelPortali P0CX43.
SMRi P0CX43. Positions 1-217.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33117. 144 interactions.
35965. 120 interactions.
IntActi P0CX43. 1 interaction.
MINTi MINT-495020.

Proteomic databases

MaxQBi P0CX43.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL135W ; YGL135W ; YGL135W .
YPL220W ; YPL220W ; YPL220W .
GeneIDi 852742.
855881.
KEGGi sce:YGL135W.
sce:YPL220W.

Organism-specific databases

CYGDi YPL220w.
SGDi S000006141. RPL1A.

Phylogenomic databases

InParanoidi P0CX43.
KOi K02865.
OrthoDBi EOG73Z358.

Enzyme and pathway databases

BioCyci YEAST:G3O-34109-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

EvolutionaryTracei P0CX43.
NextBioi 972159.

Family and domain databases

Gene3Di 3.30.190.20. 2 hits.
3.40.50.790. 1 hit.
InterProi IPR002143. Ribosomal_L1.
IPR023674. Ribosomal_L1-like.
IPR028364. Ribosomal_L1/biogenesis.
IPR016094. Ribosomal_L1_2-a/b-sand.
IPR016095. Ribosomal_L1_3-a/b-sand.
IPR023673. Ribosomal_L1_CS.
[Graphical view ]
Pfami PF00687. Ribosomal_L1. 1 hit.
[Graphical view ]
PIRSFi PIRSF002155. Ribosomal_L1. 1 hit.
SUPFAMi SSF56808. SSF56808. 1 hit.
PROSITEi PS01199. RIBOSOMAL_L1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The duplicated Saccharomyces cerevisiae gene SSM1 encodes a eucaryotic homolog of the eubacterial and archaebacterial L1 ribosomal proteins."
    Petitjean A., Bonneaud N., Lacroute F.
    Mol. Cell. Biol. 15:5071-5081(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  5. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
  6. "Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
    Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
    Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79 AND SER-86, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "The ribosomal L1 protuberance in yeast is methylated on a lysine residue catalyzed by a seven beta-strand methyltransferase."
    Webb K.J., Al-Hadid Q., Zurita-Lopez C.I., Young B.D., Lipson R.S., Clarke S.G.
    J. Biol. Chem. 286:18405-18413(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT LYS-47, IDENTIFICATION BY MASS SPECTROMETRY.
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 4-216, ELECTRON MICROSCOPY.
  15. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
  16. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.3 ANGSTROMS) OF 4-216.

Entry informationi

Entry nameiRL1A_YEAST
AccessioniPrimary (citable) accession number: P0CX43
Secondary accession number(s): D6VU14, P53030
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 26, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 116000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for L1 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3