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P0CX41 (RL23A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
60S ribosomal protein L23-A
Alternative name(s):
L17a
YL32
Gene names
Name:RPL23A
Synonyms:RPL17A, RPL17AA
Ordered Locus Names:YBL087C
ORF Names:YBL0713
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length137 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.5

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01367.

Post-translational modification

Methylated by RKM1 at 2 different sites, but it is unclear which are the 2 methylated residues among Lys-40, Lys-106 and/or Lys-110. Ref.7

Miscellaneous

There are 2 genes for L23 in yeast.

Sequence similarities

Belongs to the ribosomal protein L14P family.

Mass spectrometry

Molecular mass is 14430.702 Da from positions 2 - 137. Determined by ESI. Monoisotopic mass with either 7 methylation modifications or 1 acetylation and 4 methylation modifications. Ref.6

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionRibonucleoprotein
Ribosomal protein
   PTMAcetylation
Methylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcytoplasmic translation

Inferred by curator Ref.6. Source: SGD

   Cellular_componentcytosolic large ribosomal subunit

Inferred from direct assay Ref.6. Source: SGD

   Molecular_functionstructural constituent of ribosome

Inferred by curator Ref.6. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed HAMAP-Rule MF_01367
Chain2 – 13713660S ribosomal protein L23-A HAMAP-Rule MF_01367
PRO_0000128630

Amino acid modifications

Modified residue21N-acetylserine Ref.7 Ref.8
Modified residue401N6,N6-dimethyllysine; by RKM1 Probable
Modified residue1061N6,N6-dimethyllysine; by RKM1 Probable
Modified residue1101N6,N6-dimethyllysine; by RKM1 Probable

Sequences

Sequence LengthMass (Da)Tools
P0CX41 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: DEB983B3CB1DFAB1

FASTA13714,473
        10         20         30         40         50         60 
MSGNGAQGTK FRISLGLPVG AIMNCADNSG ARNLYIIAVK GSGSRLNRLP AASLGDMVMA 

        70         80         90        100        110        120 
TVKKGKPELR KKVMPAIVVR QAKSWRRRDG VFLYFEDNAG VIANPKGEMK GSAITGPVGK 

       130 
ECADLWPRVA SNSGVVV 

« Hide

References

« Hide 'large scale' references
[1]"Structural comparison of yeast ribosomal protein genes."
Leer R.J., van Raamsdonk-Duin M.M.C., Hagendoorn M.J.M., Mager W.H., Planta R.J.
Nucleic Acids Res. 12:6685-6700(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[6]"Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR."
Lee S.-W., Berger S.J., Martinovic S., Pasa-Tolic L., Anderson G.A., Shen Y., Zhao R., Smith R.D.
Proc. Natl. Acad. Sci. U.S.A. 99:5942-5947(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
[7]"A novel SET domain methyltransferase modifies ribosomal protein Rpl23ab in yeast."
Porras-Yakushi T.R., Whitelegge J.P., Miranda T.B., Clarke S.
J. Biol. Chem. 280:34590-34598(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION AT SER-2, METHYLATION AT LYS-40; LYS-106 AND LYS-110.
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 7-137, ELECTRON MICROSCOPY.
[10]"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
[11]"Crystal structure of the eukaryotic ribosome."
Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
[12]"The structure of the eukaryotic ribosome at 3.0 A resolution."
Ben-Shem A., Garreau de Loubresse N., Melnikov S., Jenner L., Yusupova G., Yusupov M.
Science 334:1524-1529(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X01694 Genomic DNA. Translation: CAA25841.1.
X79489 Genomic DNA. Translation: CAA56018.1.
Z35848 Genomic DNA. Translation: CAA84908.1.
BK006936 Genomic DNA. Translation: DAA07037.1.
PIRR5BY17. A02792.
RefSeqNP_009466.1. NM_001178327.1.
NP_011042.3. NM_001179007.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-R7-137[»]
1S1Ielectron microscopy11.70R1-137[»]
2X7Nelectron microscopy11.80C6-137[»]
3IZSelectron microscopy-M1-137[»]
3J16electron microscopy-I1-137[»]
3J65electron microscopy8.70L1-137[»]
3O58X-ray4.00U1-137[»]
3O5HX-ray4.00U1-137[»]
3U5EX-ray3.00V1-137[»]
3U5IX-ray3.00V1-137[»]
4B6Aelectron microscopy8.10V1-137[»]
4BYNelectron microscopy4.30V2-137[»]
4BYUelectron microscopy6.60V2-137[»]
ProteinModelPortalP0CX41.
SMRP0CX41. Positions 2-137.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32617. 91 interactions.
36862. 25 interactions.
IntActP0CX41. 1 interaction.
MINTMINT-515434.

Proteomic databases

MaxQBP0CX41.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL087C; YBL087C; YBL087C.
YER117W; YER117W; YER117W.
GeneID852191.
856853.
KEGGsce:YBL087C.
sce:YER117W.

Organism-specific databases

CYGDYBL087c.
SGDS000000183. RPL23A.

Phylogenomic databases

HOGENOMHOG000183703.
KOK02894.
OrthoDBEOG7N0CHC.

Enzyme and pathway databases

BioCycYEAST:G3O-28976-MONOMER.

Family and domain databases

Gene3D2.40.150.20. 1 hit.
HAMAPMF_01367. Ribosomal_L14.
InterProIPR019972. Ribosomal_L14_CS.
IPR023571. Ribosomal_L14_dom.
IPR000218. Ribosomal_L14b/L23e.
[Graphical view]
PANTHERPTHR11761. PTHR11761. 1 hit.
PfamPF00238. Ribosomal_L14. 1 hit.
[Graphical view]
SUPFAMSSF50193. SSF50193. 1 hit.
PROSITEPS00049. RIBOSOMAL_L14. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0CX41.
NextBio970670.

Entry information

Entry nameRL23A_YEAST
AccessionPrimary (citable) accession number: P0CX41
Secondary accession number(s): D3DM23, P04451
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: May 14, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references