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Protein

40S ribosomal protein S8-A

Gene

RPS8A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-28965-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S8-A
Alternative name(s):
RP19
S14
YS9
Gene namesi
Name:RPS8A
Synonyms:RPS14A
Ordered Locus Names:YBL072C
ORF Names:YBL06.05, YBL0613
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

SGDiS000000168. RPS8A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 20019940S ribosomal protein S8-APRO_0000122258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621PhosphothreonineCombined sources
Modified residuei66 – 661PhosphoserineCombined sources
Modified residuei69 – 691PhosphoserineCombined sources
Modified residuei73 – 731PhosphoserineCombined sources
Modified residuei86 – 861PhosphoserineCombined sources
Modified residuei107 – 1071PhosphothreonineCombined sources
Modified residuei154 – 1541PhosphoserineCombined sources
Modified residuei155 – 1551PhosphoserineCombined sources
Modified residuei158 – 1581PhosphoserineCombined sources
Modified residuei161 – 1611PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CX39.

PTM databases

iPTMnetiP0CX39.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX39. differential.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi32629. 235 interactions.
36848. 27 interactions.
IntActiP0CX39. 2 interactions.
MINTiMINT-411055.

Structurei

Secondary structure

1
200
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 104Combined sources
Turni13 – 153Combined sources
Helixi26 – 283Combined sources
Beta strandi38 – 403Combined sources
Beta strandi43 – 475Combined sources
Beta strandi49 – 513Combined sources
Beta strandi53 – 575Combined sources
Beta strandi62 – 676Combined sources
Helixi68 – 703Combined sources
Beta strandi72 – 776Combined sources
Beta strandi80 – 834Combined sources
Helixi89 – 924Combined sources
Beta strandi101 – 1033Combined sources
Helixi107 – 11610Combined sources
Helixi138 – 1458Combined sources
Beta strandi147 – 1493Combined sources
Helixi154 – 1607Combined sources
Turni161 – 1633Combined sources
Beta strandi166 – 1694Combined sources
Helixi173 – 1764Combined sources
Beta strandi181 – 1833Combined sources
Helixi186 – 19712Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10S81-200[»]
3J6Yelectron microscopy6.10S81-200[»]
3J77electron microscopy6.20S81-200[»]
3J78electron microscopy6.30S81-200[»]
3V88X-ray3.00I1-200[»]
4U3MX-ray3.00S8/s81-200[»]
4U3NX-ray3.20S8/s81-200[»]
4U3UX-ray2.90S8/s81-200[»]
4U4NX-ray3.10S8/s81-200[»]
4U4OX-ray3.60S8/s81-200[»]
4U4QX-ray3.00S8/s81-200[»]
4U4RX-ray2.80S8/s81-200[»]
4U4UX-ray3.00S8/s81-200[»]
4U4YX-ray3.20S8/s81-200[»]
4U4ZX-ray3.10S8/s81-200[»]
4U50X-ray3.20S8/s81-200[»]
4U51X-ray3.20S8/s81-200[»]
4U52X-ray3.00S8/s81-200[»]
4U53X-ray3.30S8/s81-200[»]
4U55X-ray3.20S8/s81-200[»]
4U56X-ray3.45S8/s81-200[»]
4U6FX-ray3.10S8/s81-200[»]
4V88X-ray3.00AI/CI1-200[»]
4V8Yelectron microscopy4.30AI1-200[»]
4V8Zelectron microscopy6.60AI1-200[»]
4V92electron microscopy3.70I2-199[»]
ProteinModelPortaliP0CX39.
SMRiP0CX39. Positions 2-200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S8e family.Curated

Phylogenomic databases

HOGENOMiHOG000211544.
InParanoidiP0CX39.
KOiK02995.
OrthoDBiEOG780S00.

Family and domain databases

InterProiIPR001047. Ribosomal_S8e.
IPR022309. Ribosomal_S8e/biogenesis_NSA2.
IPR018283. Ribosomal_S8e_CS.
[Graphical view]
PANTHERiPTHR10394. PTHR10394. 1 hit.
PfamiPF01201. Ribosomal_S8e. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00307. eS8. 1 hit.
PROSITEiPS01193. RIBOSOMAL_S8E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG
60 70 80 90 100
GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNEL VRTNTLTKAA
110 120 130 140 150
IVQIDATPFR QWFEAHYGQT LGKKKNVKEE ETVAKSKNAE RKWAARAASA
160 170 180 190 200
KIESSVESQF SAGRLYACIS SRPGQSGRCD GYILEGEELA FYLRRLTAKK
Length:200
Mass (Da):22,490
Last modified:June 28, 2011 - v1
Checksum:i3DB5CD6B4970C457
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491R → K AA sequence (PubMed:18782943).Curated
Sequence conflicti101 – 1022IV → MS in ABM97467 (PubMed:17244705).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26879 Genomic DNA. Translation: CAA81525.1.
Z35833 Genomic DNA. Translation: CAA84893.1.
EF123123 mRNA. Translation: ABM97467.1.
BK006936 Genomic DNA. Translation: DAA07049.1.
PIRiS45591.
RefSeqiNP_009481.1. NM_001178312.1.
NP_011028.1. NM_001178993.1.

Genome annotation databases

EnsemblFungiiYBL072C; YBL072C; YBL072C.
YER102W; YER102W; YER102W.
GeneIDi852206.
856839.
KEGGisce:YBL072C.
sce:YER102W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26879 Genomic DNA. Translation: CAA81525.1.
Z35833 Genomic DNA. Translation: CAA84893.1.
EF123123 mRNA. Translation: ABM97467.1.
BK006936 Genomic DNA. Translation: DAA07049.1.
PIRiS45591.
RefSeqiNP_009481.1. NM_001178312.1.
NP_011028.1. NM_001178993.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10S81-200[»]
3J6Yelectron microscopy6.10S81-200[»]
3J77electron microscopy6.20S81-200[»]
3J78electron microscopy6.30S81-200[»]
3V88X-ray3.00I1-200[»]
4U3MX-ray3.00S8/s81-200[»]
4U3NX-ray3.20S8/s81-200[»]
4U3UX-ray2.90S8/s81-200[»]
4U4NX-ray3.10S8/s81-200[»]
4U4OX-ray3.60S8/s81-200[»]
4U4QX-ray3.00S8/s81-200[»]
4U4RX-ray2.80S8/s81-200[»]
4U4UX-ray3.00S8/s81-200[»]
4U4YX-ray3.20S8/s81-200[»]
4U4ZX-ray3.10S8/s81-200[»]
4U50X-ray3.20S8/s81-200[»]
4U51X-ray3.20S8/s81-200[»]
4U52X-ray3.00S8/s81-200[»]
4U53X-ray3.30S8/s81-200[»]
4U55X-ray3.20S8/s81-200[»]
4U56X-ray3.45S8/s81-200[»]
4U6FX-ray3.10S8/s81-200[»]
4V88X-ray3.00AI/CI1-200[»]
4V8Yelectron microscopy4.30AI1-200[»]
4V8Zelectron microscopy6.60AI1-200[»]
4V92electron microscopy3.70I2-199[»]
ProteinModelPortaliP0CX39.
SMRiP0CX39. Positions 2-200.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32629. 235 interactions.
36848. 27 interactions.
IntActiP0CX39. 2 interactions.
MINTiMINT-411055.

PTM databases

iPTMnetiP0CX39.

Proteomic databases

MaxQBiP0CX39.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL072C; YBL072C; YBL072C.
YER102W; YER102W; YER102W.
GeneIDi852206.
856839.
KEGGisce:YBL072C.
sce:YER102W.

Organism-specific databases

SGDiS000000168. RPS8A.

Phylogenomic databases

HOGENOMiHOG000211544.
InParanoidiP0CX39.
KOiK02995.
OrthoDBiEOG780S00.

Enzyme and pathway databases

BioCyciYEAST:G3O-28965-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

NextBioi970706.
PROiP0CX39.

Gene expression databases

ExpressionAtlasiP0CX39. differential.

Family and domain databases

InterProiIPR001047. Ribosomal_S8e.
IPR022309. Ribosomal_S8e/biogenesis_NSA2.
IPR018283. Ribosomal_S8e_CS.
[Graphical view]
PANTHERiPTHR10394. PTHR10394. 1 hit.
PfamiPF01201. Ribosomal_S8e. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00307. eS8. 1 hit.
PROSITEiPS01193. RIBOSOMAL_S8E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The two genes encoding yeast ribosomal protein S8 reside on different chromosomes, and are closely linked to the hsp70 stress protein genes SSA3 and SSA4."
    Logghe M., Molemans F., Fiers W., Contreras R.
    Yeast 10:1093-1100(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-102.
    Strain: ATCC 201390 / BY4743.
  5. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
    Otaka E., Higo K., Itoh T.
    Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE OF 2-51, CLEAVAGE OF INITIATOR METHIONINE.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66; SER-69 AND SER-73, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-107 AND SER-158, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-62; SER-69; SER-73; SER-86; SER-154; SER-155 AND SER-161, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRS8A_YEAST
AccessioniPrimary (citable) accession number: P0CX39
Secondary accession number(s): A2TBM0
, A2TBP0, D3DM09, P05754, P22801
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 13, 2016
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15900 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S8 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.