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Protein

40S ribosomal protein S8-A

Gene

RPS8A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-28965-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S8-A
Alternative name(s):
RP19
S14
YS9
Gene namesi
Name:RPS8A
Synonyms:RPS14A
Ordered Locus Names:YBL072C
ORF Names:YBL06.05, YBL0613
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

SGDiS000000168. RPS8A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001222582 – 20040S ribosomal protein S8-AAdd BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei62PhosphothreonineCombined sources1
Modified residuei66PhosphoserineCombined sources1
Modified residuei69PhosphoserineCombined sources1
Modified residuei73PhosphoserineCombined sources1
Modified residuei86PhosphoserineCombined sources1
Modified residuei107PhosphothreonineCombined sources1
Modified residuei154PhosphoserineCombined sources1
Modified residuei155PhosphoserineCombined sources1
Modified residuei158PhosphoserineCombined sources1
Modified residuei161PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CX39.
PRIDEiP0CX39.

PTM databases

iPTMnetiP0CX39.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX39. baseline.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi32629. 236 interactors.
36848. 28 interactors.
IntActiP0CX39. 2 interactors.
MINTiMINT-411055.

Structurei

Secondary structure

1200
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 10Combined sources4
Turni13 – 15Combined sources3
Helixi26 – 28Combined sources3
Beta strandi38 – 40Combined sources3
Beta strandi43 – 47Combined sources5
Beta strandi49 – 51Combined sources3
Beta strandi53 – 57Combined sources5
Beta strandi62 – 67Combined sources6
Helixi68 – 70Combined sources3
Beta strandi72 – 77Combined sources6
Beta strandi80 – 83Combined sources4
Helixi89 – 92Combined sources4
Beta strandi101 – 103Combined sources3
Helixi107 – 116Combined sources10
Helixi138 – 145Combined sources8
Beta strandi147 – 149Combined sources3
Helixi154 – 160Combined sources7
Turni161 – 163Combined sources3
Beta strandi166 – 169Combined sources4
Helixi173 – 176Combined sources4
Beta strandi181 – 183Combined sources3
Helixi186 – 197Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10S81-200[»]
3J6Yelectron microscopy6.10S81-200[»]
3J77electron microscopy6.20S81-200[»]
3J78electron microscopy6.30S81-200[»]
3V88X-ray3.00I1-200[»]
4U3MX-ray3.00S8/s81-200[»]
4U3NX-ray3.20S8/s81-200[»]
4U3UX-ray2.90S8/s81-200[»]
4U4NX-ray3.10S8/s81-200[»]
4U4OX-ray3.60S8/s81-200[»]
4U4QX-ray3.00S8/s81-200[»]
4U4RX-ray2.80S8/s81-200[»]
4U4UX-ray3.00S8/s81-200[»]
4U4YX-ray3.20S8/s81-200[»]
4U4ZX-ray3.10S8/s81-200[»]
4U50X-ray3.20S8/s81-200[»]
4U51X-ray3.20S8/s81-200[»]
4U52X-ray3.00S8/s81-200[»]
4U53X-ray3.30S8/s81-200[»]
4U55X-ray3.20S8/s81-200[»]
4U56X-ray3.45S8/s81-200[»]
4U6FX-ray3.10S8/s81-200[»]
4V88X-ray3.00AI/CI1-200[»]
4V8Yelectron microscopy4.30AI1-200[»]
4V8Zelectron microscopy6.60AI1-200[»]
4V92electron microscopy3.70I2-199[»]
5DATX-ray3.15S8/s81-200[»]
5DC3X-ray3.25S8/s81-200[»]
5FCIX-ray3.40S8/s81-200[»]
5FCJX-ray3.10S8/s81-200[»]
5I4LX-ray3.10S8/s81-200[»]
5JUOelectron microscopy4.00FB1-200[»]
5JUPelectron microscopy3.50FB1-200[»]
5JUSelectron microscopy4.20FB1-200[»]
5JUTelectron microscopy4.00FB1-200[»]
5JUUelectron microscopy4.00FB1-200[»]
ProteinModelPortaliP0CX39.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S8e family.Curated

Phylogenomic databases

HOGENOMiHOG000211544.
InParanoidiP0CX39.
KOiK02995.
OrthoDBiEOG092C59CG.

Family and domain databases

InterProiIPR001047. Ribosomal_S8e.
IPR022309. Ribosomal_S8e/biogenesis_NSA2.
IPR018283. Ribosomal_S8e_CS.
[Graphical view]
PANTHERiPTHR10394. PTHR10394. 1 hit.
PfamiPF01201. Ribosomal_S8e. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00307. eS8. 1 hit.
PROSITEiPS01193. RIBOSOMAL_S8E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG
60 70 80 90 100
GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNEL VRTNTLTKAA
110 120 130 140 150
IVQIDATPFR QWFEAHYGQT LGKKKNVKEE ETVAKSKNAE RKWAARAASA
160 170 180 190 200
KIESSVESQF SAGRLYACIS SRPGQSGRCD GYILEGEELA FYLRRLTAKK
Length:200
Mass (Da):22,490
Last modified:June 28, 2011 - v1
Checksum:i3DB5CD6B4970C457
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49R → K AA sequence (PubMed:18782943).Curated1
Sequence conflicti101 – 102IV → MS in ABM97467 (PubMed:17244705).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26879 Genomic DNA. Translation: CAA81525.1.
Z35833 Genomic DNA. Translation: CAA84893.1.
EF123123 mRNA. Translation: ABM97467.1.
BK006936 Genomic DNA. Translation: DAA07049.1.
PIRiS45591.
RefSeqiNP_009481.1. NM_001178312.1.
NP_011028.1. NM_001178993.1.

Genome annotation databases

EnsemblFungiiYBL072C; YBL072C; YBL072C.
YER102W; YER102W; YER102W.
GeneIDi852206.
856839.
KEGGisce:YBL072C.
sce:YER102W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26879 Genomic DNA. Translation: CAA81525.1.
Z35833 Genomic DNA. Translation: CAA84893.1.
EF123123 mRNA. Translation: ABM97467.1.
BK006936 Genomic DNA. Translation: DAA07049.1.
PIRiS45591.
RefSeqiNP_009481.1. NM_001178312.1.
NP_011028.1. NM_001178993.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10S81-200[»]
3J6Yelectron microscopy6.10S81-200[»]
3J77electron microscopy6.20S81-200[»]
3J78electron microscopy6.30S81-200[»]
3V88X-ray3.00I1-200[»]
4U3MX-ray3.00S8/s81-200[»]
4U3NX-ray3.20S8/s81-200[»]
4U3UX-ray2.90S8/s81-200[»]
4U4NX-ray3.10S8/s81-200[»]
4U4OX-ray3.60S8/s81-200[»]
4U4QX-ray3.00S8/s81-200[»]
4U4RX-ray2.80S8/s81-200[»]
4U4UX-ray3.00S8/s81-200[»]
4U4YX-ray3.20S8/s81-200[»]
4U4ZX-ray3.10S8/s81-200[»]
4U50X-ray3.20S8/s81-200[»]
4U51X-ray3.20S8/s81-200[»]
4U52X-ray3.00S8/s81-200[»]
4U53X-ray3.30S8/s81-200[»]
4U55X-ray3.20S8/s81-200[»]
4U56X-ray3.45S8/s81-200[»]
4U6FX-ray3.10S8/s81-200[»]
4V88X-ray3.00AI/CI1-200[»]
4V8Yelectron microscopy4.30AI1-200[»]
4V8Zelectron microscopy6.60AI1-200[»]
4V92electron microscopy3.70I2-199[»]
5DATX-ray3.15S8/s81-200[»]
5DC3X-ray3.25S8/s81-200[»]
5FCIX-ray3.40S8/s81-200[»]
5FCJX-ray3.10S8/s81-200[»]
5I4LX-ray3.10S8/s81-200[»]
5JUOelectron microscopy4.00FB1-200[»]
5JUPelectron microscopy3.50FB1-200[»]
5JUSelectron microscopy4.20FB1-200[»]
5JUTelectron microscopy4.00FB1-200[»]
5JUUelectron microscopy4.00FB1-200[»]
ProteinModelPortaliP0CX39.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32629. 236 interactors.
36848. 28 interactors.
IntActiP0CX39. 2 interactors.
MINTiMINT-411055.

PTM databases

iPTMnetiP0CX39.

Proteomic databases

MaxQBiP0CX39.
PRIDEiP0CX39.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL072C; YBL072C; YBL072C.
YER102W; YER102W; YER102W.
GeneIDi852206.
856839.
KEGGisce:YBL072C.
sce:YER102W.

Organism-specific databases

SGDiS000000168. RPS8A.

Phylogenomic databases

HOGENOMiHOG000211544.
InParanoidiP0CX39.
KOiK02995.
OrthoDBiEOG092C59CG.

Enzyme and pathway databases

BioCyciYEAST:G3O-28965-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiP0CX39.

Gene expression databases

ExpressionAtlasiP0CX39. baseline.

Family and domain databases

InterProiIPR001047. Ribosomal_S8e.
IPR022309. Ribosomal_S8e/biogenesis_NSA2.
IPR018283. Ribosomal_S8e_CS.
[Graphical view]
PANTHERiPTHR10394. PTHR10394. 1 hit.
PfamiPF01201. Ribosomal_S8e. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00307. eS8. 1 hit.
PROSITEiPS01193. RIBOSOMAL_S8E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRS8A_YEAST
AccessioniPrimary (citable) accession number: P0CX39
Secondary accession number(s): A2TBM0
, A2TBP0, D3DM09, P05754, P22801
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 30, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15900 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S8 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.