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Protein

40S ribosomal protein S6-A

Gene

RPS6A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.1 Publication

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33995-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-166208. mTORC1-mediated signalling.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S6-A
Alternative name(s):
RP9
S10
YS4
Gene namesi
Name:RPS6A
Synonyms:RPS101, RPS10B
Ordered Locus Names:YPL090C
ORF Names:LPG18C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

SGDiS000006011. RPS6A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
  • nucleolus Source: UniProtKB-SubCell
  • small-subunit processome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 23623640S ribosomal protein S6-APRO_0000137342Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei163 – 1631PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CX37.

PTM databases

iPTMnetiP0CX37.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX37. differential.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.2 Publications

Protein-protein interaction databases

BioGridi32879. 105 interactions.
36091. 110 interactions.
IntActiP0CX37. 2 interactions.
MINTiMINT-8285675.

Structurei

Secondary structure

1
236
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Turni8 – 114Combined sources
Beta strandi12 – 176Combined sources
Helixi21 – 244Combined sources
Helixi25 – 273Combined sources
Beta strandi31 – 333Combined sources
Beta strandi35 – 384Combined sources
Turni39 – 413Combined sources
Beta strandi43 – 453Combined sources
Beta strandi49 – 579Combined sources
Helixi58 – 603Combined sources
Beta strandi69 – 779Combined sources
Beta strandi81 – 833Combined sources
Beta strandi87 – 904Combined sources
Beta strandi93 – 986Combined sources
Beta strandi106 – 11510Combined sources
Helixi122 – 1254Combined sources
Helixi138 – 1436Combined sources
Turni144 – 1463Combined sources
Helixi148 – 1503Combined sources
Helixi153 – 1553Combined sources
Beta strandi160 – 1634Combined sources
Beta strandi168 – 1714Combined sources
Helixi181 – 21636Combined sources
Beta strandi219 – 2224Combined sources
Turni223 – 2253Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J16electron microscopy-C1-236[»]
3J6Xelectron microscopy6.10S61-236[»]
3J6Yelectron microscopy6.10S61-236[»]
3J77electron microscopy6.20S61-236[»]
3J78electron microscopy6.30S61-236[»]
3V88X-ray3.00G1-236[»]
4U3MX-ray3.00S6/s61-236[»]
4U3NX-ray3.20S6/s61-236[»]
4U3UX-ray2.90S6/s61-236[»]
4U4NX-ray3.10S6/s61-236[»]
4U4OX-ray3.60S6/s61-236[»]
4U4QX-ray3.00S6/s61-236[»]
4U4RX-ray2.80S6/s61-236[»]
4U4UX-ray3.00S6/s61-236[»]
4U4YX-ray3.20S6/s61-236[»]
4U4ZX-ray3.10S6/s61-236[»]
4U50X-ray3.20S6/s61-236[»]
4U51X-ray3.20S6/s61-236[»]
4U52X-ray3.00S6/s61-236[»]
4U53X-ray3.30S6/s61-236[»]
4U55X-ray3.20S6/s61-236[»]
4U56X-ray3.45S6/s61-236[»]
4U6FX-ray3.10S6/s61-236[»]
4V88X-ray3.00AG/CG1-236[»]
4V8Yelectron microscopy4.30AG1-236[»]
4V8Zelectron microscopy6.60AG1-236[»]
4V92electron microscopy3.70G1-226[»]
ProteinModelPortaliP0CX37.
SMRiP0CX37. Positions 1-226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S6e family.Curated

Phylogenomic databases

InParanoidiP0CX37.
KOiK02991.
OrthoDBiEOG71GB67.

Family and domain databases

InterProiIPR014401. Ribosomal_S6_euk.
IPR001377. Ribosomal_S6e.
IPR018282. Ribosomal_S6e_CS.
[Graphical view]
PANTHERiPTHR11502. PTHR11502. 1 hit.
PfamiPF01092. Ribosomal_S6e. 1 hit.
[Graphical view]
PIRSFiPIRSF002129. Ribosom_S6_euk. 1 hit.
SMARTiSM01405. Ribosomal_S6e. 1 hit.
[Graphical view]
PROSITEiPS00578. RIBOSOMAL_S6E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CX37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF
60 70 80 90 100
KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRDG ERKRKSVRGA
110 120 130 140 150
IVGPDLAVLA LVIVKKGEQE LEGLTDTTVP KRLGPKRANN IRKFFGLSKE
160 170 180 190 200
DDVRDFVIRR EVTKGEKTYT KAPKIQRLVT PQRLQRKRHQ RALKVRNAQA
210 220 230
QREAAAEYAQ LLAKRLSERK AEKAEIRKRR ASSLKA
Length:236
Mass (Da):26,996
Last modified:June 28, 2011 - v1
Checksum:i78B523BB7FDABEE0
GO

Sequence cautioni

The sequence AAB68209.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02746 Genomic DNA. Translation: CAA26525.1.
U43281 Genomic DNA. Translation: AAB68209.1. Sequence problems.
BK006949 Genomic DNA. Translation: DAA11343.1.
PIRiA23787. R3BY10.
RefSeqiNP_009740.3. NM_001178529.3.
NP_015235.1. NM_001183904.1.

Genome annotation databases

EnsemblFungiiYBR181C; YBR181C; YBR181C.
YPL090C; YPL090C; YPL090C.
GeneIDi852479.
856015.
KEGGisce:YBR181C.
sce:YPL090C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02746 Genomic DNA. Translation: CAA26525.1.
U43281 Genomic DNA. Translation: AAB68209.1. Sequence problems.
BK006949 Genomic DNA. Translation: DAA11343.1.
PIRiA23787. R3BY10.
RefSeqiNP_009740.3. NM_001178529.3.
NP_015235.1. NM_001183904.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J16electron microscopy-C1-236[»]
3J6Xelectron microscopy6.10S61-236[»]
3J6Yelectron microscopy6.10S61-236[»]
3J77electron microscopy6.20S61-236[»]
3J78electron microscopy6.30S61-236[»]
3V88X-ray3.00G1-236[»]
4U3MX-ray3.00S6/s61-236[»]
4U3NX-ray3.20S6/s61-236[»]
4U3UX-ray2.90S6/s61-236[»]
4U4NX-ray3.10S6/s61-236[»]
4U4OX-ray3.60S6/s61-236[»]
4U4QX-ray3.00S6/s61-236[»]
4U4RX-ray2.80S6/s61-236[»]
4U4UX-ray3.00S6/s61-236[»]
4U4YX-ray3.20S6/s61-236[»]
4U4ZX-ray3.10S6/s61-236[»]
4U50X-ray3.20S6/s61-236[»]
4U51X-ray3.20S6/s61-236[»]
4U52X-ray3.00S6/s61-236[»]
4U53X-ray3.30S6/s61-236[»]
4U55X-ray3.20S6/s61-236[»]
4U56X-ray3.45S6/s61-236[»]
4U6FX-ray3.10S6/s61-236[»]
4V88X-ray3.00AG/CG1-236[»]
4V8Yelectron microscopy4.30AG1-236[»]
4V8Zelectron microscopy6.60AG1-236[»]
4V92electron microscopy3.70G1-226[»]
ProteinModelPortaliP0CX37.
SMRiP0CX37. Positions 1-226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32879. 105 interactions.
36091. 110 interactions.
IntActiP0CX37. 2 interactions.
MINTiMINT-8285675.

PTM databases

iPTMnetiP0CX37.

Proteomic databases

MaxQBiP0CX37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR181C; YBR181C; YBR181C.
YPL090C; YPL090C; YPL090C.
GeneIDi852479.
856015.
KEGGisce:YBR181C.
sce:YPL090C.

Organism-specific databases

SGDiS000006011. RPS6A.

Phylogenomic databases

InParanoidiP0CX37.
KOiK02991.
OrthoDBiEOG71GB67.

Enzyme and pathway databases

BioCyciYEAST:G3O-33995-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-166208. mTORC1-mediated signalling.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

NextBioi971447.
PROiP0CX37.

Gene expression databases

ExpressionAtlasiP0CX37. differential.

Family and domain databases

InterProiIPR014401. Ribosomal_S6_euk.
IPR001377. Ribosomal_S6e.
IPR018282. Ribosomal_S6e_CS.
[Graphical view]
PANTHERiPTHR11502. PTHR11502. 1 hit.
PfamiPF01092. Ribosomal_S6e. 1 hit.
[Graphical view]
PIRSFiPIRSF002129. Ribosom_S6_euk. 1 hit.
SMARTiSM01405. Ribosomal_S6e. 1 hit.
[Graphical view]
PROSITEiPS00578. RIBOSOMAL_S6E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Carlsbergensis.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast."
    Otaka E., Higo K., Osawa S.
    Biochemistry 21:4545-4550(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 1-30.
  5. "Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe."
    Otaka E., Higo K., Itoh T.
    Mol. Gen. Genet. 191:519-524(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 1.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "The small-subunit processome is a ribosome assembly intermediate."
    Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J.
    Eukaryot. Cell 3:1619-1626(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.
  10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-163, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRS6A_YEAST
AccessioniPrimary (citable) accession number: P0CX37
Secondary accession number(s): D6VQH5
, P02365, P05751, Q02894
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: May 11, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 66000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S6 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.