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P0CX36 (RS4B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
40S ribosomal protein S4-B
Alternative name(s):
RP5
S7
YS6
Gene names
Name:RPS4B
Synonyms:RPS7A
Ordered Locus Names:YHR203C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6

Subcellular location

Cytoplasm Ref.7.

Miscellaneous

Present with 102000 molecules/cell in log phase SD medium.

There are 2 genes for S4 in yeast.

Sequence similarities

Belongs to the ribosomal protein S4e family.

Contains 1 S4 RNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 26126040S ribosomal protein S4-B HAMAP-Rule MF_00485
PRO_0000409764

Regions

Domain42 – 10564S4 RNA-binding

Amino acid modifications

Modified residue321Phosphoserine Ref.11
Modified residue1151Phosphothreonine Ref.12
Modified residue2471Phosphoserine Ref.10
Cross-link168Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.9

Sequences

Sequence LengthMass (Da)Tools
P0CX36 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: F109594560F34734

FASTA26129,410
        10         20         30         40         50         60 
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE 

        70         80         90        100        110        120 
VKAILMQRHV KVDGKVRTDT TYPAGFMDVI TLDATNENFR LVYDVKGRFA VHRITDEEAS 

       130        140        150        160        170        180 
YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP DPNIKVNDTV KIDLASGKIT DFIKFDAGKL 

       190        200        210        220        230        240 
VYVTGGRNLG RIGTIVHKER HDGGFDLVHI KDSLDNTFVT RLNNVFVIGE QGKPYISLPK 

       250        260 
GKGIKLSIAE ERDRRRAQQG L 

« Hide

References

« Hide 'large scale' references
[1]"The yeast ribosomal protein S7 and its genes."
Synetos D., Dabeva M.D., Warner J.R.
J. Biol. Chem. 267:3008-3013(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Jia Y., Cherry J.M.
Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 5.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast."
Otaka E., Higo K., Osawa S.
Biochemistry 21:4545-4550(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE OF 2-29, CLEAVAGE OF INITIATOR METHIONINE.
[6]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-168.
Strain: SUB592.
[10]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[11]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[12]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-115, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64294 Genomic DNA. Translation: AAA35012.1.
U00030 Genomic DNA. Translation: AAB68372.1.
BK006934 Genomic DNA. Translation: DAA06896.1.
PIRS20054.
RefSeqNP_012073.1. NM_001179334.1.
NP_012679.3. NM_001181803.3.

3D structure databases

ProteinModelPortalP0CX36.
SMRP0CX36. Positions 2-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33901. 168 interactions.
36637. 187 interactions.
IntActP0CX36. 1 interaction.
MINTMINT-541365.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHR203C; YHR203C; YHR203C.
YJR145C; YJR145C; YJR145C.
GeneID853610.
856610.
KEGGsce:YHR203C.
sce:YJR145C.

Organism-specific databases

CYGDYHR203c.
SGDS000001246. RPS4B.

Phylogenomic databases

GeneTreeENSGT00390000005569.
KOK02987.
OrthoDBEOG7JDR84.

Enzyme and pathway databases

BioCycYEAST:G3O-31230-MONOMER.

Family and domain databases

HAMAPMF_00485. Ribosomal_S4e.
InterProIPR005824. KOW.
IPR000876. Ribosomal_S4e.
IPR013845. Ribosomal_S4e_central_region.
IPR013843. Ribosomal_S4e_N.
IPR018199. Ribosomal_S4e_N_CS.
IPR002942. S4_RNA-bd.
[Graphical view]
PANTHERPTHR11581. PTHR11581. 1 hit.
PfamPF00467. KOW. 1 hit.
PF00900. Ribosomal_S4e. 1 hit.
PF08071. RS4NT. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
PIRSFPIRSF002116. Ribosomal_S4. 1 hit.
ProDomPD002667. Ribosomal_S4e_central. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00739. KOW. 1 hit.
SM00363. S4. 1 hit.
[Graphical view]
PROSITEPS00528. RIBOSOMAL_S4E. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974454.
PROP0CX36.

Entry information

Entry nameRS4B_YEAST
AccessionPrimary (citable) accession number: P0CX36
Secondary accession number(s): D3DLF2, P05753
Entry history
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 16, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries