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Protein

40S ribosomal protein S4-B

Gene

RPS4B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 102000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eS4 in yeast.Curated

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • rRNA binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31230-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-SCE-72702 Ribosomal scanning and start codon recognition
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S4-B1 Publication
Alternative name(s):
RP5
S7
Small ribosomal subunit protein eS4-B1 Publication
YS6
Gene namesi
Name:RPS4B1 Publication
Synonyms:RPS7A
Ordered Locus Names:YHR203C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

SGDiS000001246 RPS4B

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00004097642 – 26140S ribosomal protein S4-BAdd BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphoserineCombined sources1
Cross-linki62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei115PhosphothreonineCombined sources1
Cross-linki134Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki161Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki168Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki174Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki179Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki233Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei247PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP0CX36

PTM databases

CarbonylDBiP0CX36
iPTMnetiP0CX36

Expressioni

Gene expression databases

ExpressionAtlasiP0CX36 differential

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi33901, 157 interactors
36637, 304 interactors
IntActiP0CX36, 2 interactors
MINTiP0CX36

Structurei

3D structure databases

ProteinModelPortaliP0CX36
SMRiP0CX36
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 105S4 RNA-bindingAdd BLAST64

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000005569
InParanoidiP0CX36
KOiK02987
OrthoDBiEOG092C443N

Family and domain databases

CDDicd00165 S4, 1 hit
Gene3Di2.30.30.30, 1 hit
2.40.50.740, 1 hit
HAMAPiMF_00485 Ribosomal_S4e, 1 hit
InterProiView protein in InterPro
IPR032277 40S_S4_C
IPR005824 KOW
IPR014722 Rib_L2_dom2
IPR000876 Ribosomal_S4e
IPR013845 Ribosomal_S4e_central_region
IPR038237 Ribosomal_S4e_central_sf
IPR013843 Ribosomal_S4e_N
IPR018199 Ribosomal_S4e_N_CS
IPR002942 S4_RNA-bd
PANTHERiPTHR11581 PTHR11581, 1 hit
PfamiView protein in Pfam
PF16121 40S_S4_C, 1 hit
PF00467 KOW, 1 hit
PF00900 Ribosomal_S4e, 1 hit
PF08071 RS4NT, 1 hit
PF01479 S4, 1 hit
PIRSFiPIRSF002116 Ribosomal_S4, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002667 Ribosomal_S4e_central, 1 hit
SMARTiView protein in SMART
SM00363 S4, 1 hit
PROSITEiView protein in PROSITE
PS00528 RIBOSOMAL_S4E, 1 hit
PS50889 S4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN
60 70 80 90 100
RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDVI TLDATNENFR
110 120 130 140 150
LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP
160 170 180 190 200
DPNIKVNDTV KIDLASGKIT DFIKFDAGKL VYVTGGRNLG RIGTIVHKER
210 220 230 240 250
HDGGFDLVHI KDSLDNTFVT RLNNVFVIGE QGKPYISLPK GKGIKLSIAE
260
ERDRRRAQQG L
Length:261
Mass (Da):29,410
Last modified:June 28, 2011 - v1
Checksum:iF109594560F34734
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64294 Genomic DNA Translation: AAA35012.1
U00030 Genomic DNA Translation: AAB68372.1
BK006934 Genomic DNA Translation: DAA06896.1
PIRiS20054
RefSeqiNP_012073.1, NM_001179334.1
NP_012679.3, NM_001181803.3

Genome annotation databases

EnsemblFungiiYHR203C; YHR203C; YHR203C
YJR145C; YJR145C; YJR145C
GeneIDi853610
856610
KEGGisce:YHR203C
sce:YJR145C

Similar proteinsi

Entry informationi

Entry nameiRS4B_YEAST
AccessioniPrimary (citable) accession number: P0CX36
Secondary accession number(s): D3DLF2, P05753
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: May 23, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

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