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Protein

40S ribosomal protein S4-B

Gene

RPS4B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 102000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eS4 in yeast.Curated

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • rRNA binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31230-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S4-B1 Publication
Alternative name(s):
RP5
S7
Small ribosomal subunit protein eS4-B1 Publication
YS6
Gene namesi
Name:RPS4B1 Publication
Synonyms:RPS7A
Ordered Locus Names:YHR203C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

SGDiS000001246. RPS4B.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00004097642 – 26140S ribosomal protein S4-BAdd BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphoserineCombined sources1
Cross-linki62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei115PhosphothreonineCombined sources1
Cross-linki134Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki161Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki168Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki174Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki179Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki233Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei247PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP0CX36.

PTM databases

iPTMnetiP0CX36.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX36. differential.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi33901. 151 interactors.
36637. 299 interactors.
IntActiP0CX36. 2 interactors.
MINTiMINT-541365.

Structurei

3D structure databases

ProteinModelPortaliP0CX36.
SMRiP0CX36.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 105S4 RNA-bindingAdd BLAST64

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000005569.
InParanoidiP0CX36.
KOiK02987.
OrthoDBiEOG092C443N.

Family and domain databases

CDDicd00165. S4. 1 hit.
Gene3Di3.10.290.10. 2 hits.
HAMAPiMF_00485. Ribosomal_S4e. 1 hit.
InterProiView protein in InterPro
IPR032277. 40S_S4_C.
IPR005824. KOW.
IPR000876. Ribosomal_S4e.
IPR013845. Ribosomal_S4e_central_region.
IPR013843. Ribosomal_S4e_N.
IPR018199. Ribosomal_S4e_N_CS.
IPR002942. S4_RNA-bd.
IPR036986. S4_RNA-bd_sf.
PANTHERiPTHR11581. PTHR11581. 1 hit.
PfamiView protein in Pfam
PF16121. 40S_S4_C. 1 hit.
PF00467. KOW. 1 hit.
PF00900. Ribosomal_S4e. 1 hit.
PF08071. RS4NT. 1 hit.
PF01479. S4. 1 hit.
PIRSFiPIRSF002116. Ribosomal_S4. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002667. Ribosomal_S4e_central. 1 hit.
SMARTiView protein in SMART
SM00363. S4. 1 hit.
PROSITEiView protein in PROSITE
PS00528. RIBOSOMAL_S4E. 1 hit.
PS50889. S4. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN
60 70 80 90 100
RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDVI TLDATNENFR
110 120 130 140 150
LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP
160 170 180 190 200
DPNIKVNDTV KIDLASGKIT DFIKFDAGKL VYVTGGRNLG RIGTIVHKER
210 220 230 240 250
HDGGFDLVHI KDSLDNTFVT RLNNVFVIGE QGKPYISLPK GKGIKLSIAE
260
ERDRRRAQQG L
Length:261
Mass (Da):29,410
Last modified:June 28, 2011 - v1
Checksum:iF109594560F34734
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64294 Genomic DNA. Translation: AAA35012.1.
U00030 Genomic DNA. Translation: AAB68372.1.
BK006934 Genomic DNA. Translation: DAA06896.1.
PIRiS20054.
RefSeqiNP_012073.1. NM_001179334.1.
NP_012679.3. NM_001181803.3.

Genome annotation databases

EnsemblFungiiYHR203C; YHR203C; YHR203C.
YJR145C; YJR145C; YJR145C.
GeneIDi853610.
856610.
KEGGisce:YHR203C.
sce:YJR145C.

Similar proteinsi

Entry informationi

Entry nameiRS4B_YEAST
AccessioniPrimary (citable) accession number: P0CX36
Secondary accession number(s): D3DLF2, P05753
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 22, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names