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P0CX36

- RS4B_YEAST

UniProt

P0CX36 - RS4B_YEAST

Protein

40S ribosomal protein S4-B

Gene

RPS4B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. rRNA binding Source: UniProtKB-KW
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31230-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S4-B
    Alternative name(s):
    RP5
    S7
    YS6
    Gene namesi
    Name:RPS4B
    Synonyms:RPS7A
    Ordered Locus Names:YHR203C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHR203c.
    SGDiS000001246. RPS4B.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 26126040S ribosomal protein S4-BPRO_0000409764Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei32 – 321Phosphoserine1 Publication
    Modified residuei115 – 1151Phosphothreonine1 Publication
    Cross-linki168 – 168Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Modified residuei247 – 2471Phosphoserine1 Publication

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi33901. 169 interactions.
    36637. 188 interactions.
    IntActiP0CX36. 1 interaction.
    MINTiMINT-541365.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CX36.
    SMRiP0CX36. Positions 2-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 10564S4 RNA-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ribosomal protein S4e family.Curated
    Contains 1 S4 RNA-binding domain.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000005569.
    KOiK02987.
    OrthoDBiEOG7JDR84.

    Family and domain databases

    HAMAPiMF_00485. Ribosomal_S4e.
    InterProiIPR005824. KOW.
    IPR000876. Ribosomal_S4e.
    IPR013845. Ribosomal_S4e_central_region.
    IPR013843. Ribosomal_S4e_N.
    IPR018199. Ribosomal_S4e_N_CS.
    IPR002942. S4_RNA-bd.
    [Graphical view]
    PANTHERiPTHR11581. PTHR11581. 1 hit.
    PfamiPF00467. KOW. 1 hit.
    PF00900. Ribosomal_S4e. 1 hit.
    PF08071. RS4NT. 1 hit.
    PF01479. S4. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002116. Ribosomal_S4. 1 hit.
    ProDomiPD002667. Ribosomal_S4e_central. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00739. KOW. 1 hit.
    SM00363. S4. 1 hit.
    [Graphical view]
    PROSITEiPS00528. RIBOSOMAL_S4E. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CX36-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN    50
    RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDVI TLDATNENFR 100
    LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP 150
    DPNIKVNDTV KIDLASGKIT DFIKFDAGKL VYVTGGRNLG RIGTIVHKER 200
    HDGGFDLVHI KDSLDNTFVT RLNNVFVIGE QGKPYISLPK GKGIKLSIAE 250
    ERDRRRAQQG L 261
    Length:261
    Mass (Da):29,410
    Last modified:June 28, 2011 - v1
    Checksum:iF109594560F34734
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64294 Genomic DNA. Translation: AAA35012.1.
    U00030 Genomic DNA. Translation: AAB68372.1.
    BK006934 Genomic DNA. Translation: DAA06896.1.
    PIRiS20054.
    RefSeqiNP_012073.1. NM_001179334.1.
    NP_012679.3. NM_001181803.3.

    Genome annotation databases

    EnsemblFungiiYHR203C; YHR203C; YHR203C.
    YJR145C; YJR145C; YJR145C.
    GeneIDi853610.
    856610.
    KEGGisce:YHR203C.
    sce:YJR145C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64294 Genomic DNA. Translation: AAA35012.1 .
    U00030 Genomic DNA. Translation: AAB68372.1 .
    BK006934 Genomic DNA. Translation: DAA06896.1 .
    PIRi S20054.
    RefSeqi NP_012073.1. NM_001179334.1.
    NP_012679.3. NM_001181803.3.

    3D structure databases

    ProteinModelPortali P0CX36.
    SMRi P0CX36. Positions 2-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33901. 169 interactions.
    36637. 188 interactions.
    IntActi P0CX36. 1 interaction.
    MINTi MINT-541365.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR203C ; YHR203C ; YHR203C .
    YJR145C ; YJR145C ; YJR145C .
    GeneIDi 853610.
    856610.
    KEGGi sce:YHR203C.
    sce:YJR145C.

    Organism-specific databases

    CYGDi YHR203c.
    SGDi S000001246. RPS4B.

    Phylogenomic databases

    GeneTreei ENSGT00390000005569.
    KOi K02987.
    OrthoDBi EOG7JDR84.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31230-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    NextBioi 974454.
    PROi P0CX36.

    Family and domain databases

    HAMAPi MF_00485. Ribosomal_S4e.
    InterProi IPR005824. KOW.
    IPR000876. Ribosomal_S4e.
    IPR013845. Ribosomal_S4e_central_region.
    IPR013843. Ribosomal_S4e_N.
    IPR018199. Ribosomal_S4e_N_CS.
    IPR002942. S4_RNA-bd.
    [Graphical view ]
    PANTHERi PTHR11581. PTHR11581. 1 hit.
    Pfami PF00467. KOW. 1 hit.
    PF00900. Ribosomal_S4e. 1 hit.
    PF08071. RS4NT. 1 hit.
    PF01479. S4. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002116. Ribosomal_S4. 1 hit.
    ProDomi PD002667. Ribosomal_S4e_central. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00739. KOW. 1 hit.
    SM00363. S4. 1 hit.
    [Graphical view ]
    PROSITEi PS00528. RIBOSOMAL_S4E. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast ribosomal protein S7 and its genes."
      Synetos D., Dabeva M.D., Warner J.R.
      J. Biol. Chem. 267:3008-3013(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Jia Y., Cherry J.M.
      Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 5.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast."
      Otaka E., Higo K., Osawa S.
      Biochemistry 21:4545-4550(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE OF 2-29, CLEAVAGE OF INITIATOR METHIONINE.
    6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-168.
      Strain: SUB592.
    10. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    12. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-115, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRS4B_YEAST
    AccessioniPrimary (citable) accession number: P0CX36
    Secondary accession number(s): D3DLF2, P05753
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 102000 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for S4 in yeast.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Ribosomal proteins
      Ribosomal proteins families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3