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Protein

40S ribosomal protein S4-A

Gene

RPS4A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eS4 in yeast.Curated

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • rRNA binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31759-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S4-A1 Publication
Alternative name(s):
RP5
S7
Small ribosomal subunit protein eS4-A1 Publication
YS6
Gene namesi
Name:RPS4A1 Publication
Synonyms:RPS7B
Ordered Locus Names:YJR145C
ORF Names:J2186
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

SGDiS000003906. RPS4A.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001308432 – 26140S ribosomal protein S4-AAdd BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphoserineCombined sources1
Cross-linki62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei115PhosphothreonineCombined sources1
Cross-linki134Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki161Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki168Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki174Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki179Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki233Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei247PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP0CX35.
PRIDEiP0CX35.
TopDownProteomicsiP0CX35.

PTM databases

iPTMnetiP0CX35.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX35. differential.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi33901. 151 interactors.
36637. 299 interactors.
IntActiP0CX35. 7 interactors.
MINTiMINT-8285663.
STRINGi4932.YJR145C.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni11 – 13Combined sources3
Helixi16 – 18Combined sources3
Beta strandi22 – 27Combined sources6
Beta strandi33 – 36Combined sources4
Helixi38 – 40Combined sources3
Beta strandi41 – 43Combined sources3
Helixi44 – 49Combined sources6
Turni50 – 52Combined sources3
Beta strandi56 – 58Combined sources3
Helixi59 – 66Combined sources8
Beta strandi70 – 72Combined sources3
Beta strandi83 – 85Combined sources3
Beta strandi89 – 92Combined sources4
Helixi94 – 96Combined sources3
Beta strandi97 – 100Combined sources4
Beta strandi101 – 103Combined sources3
Beta strandi105 – 107Combined sources3
Beta strandi112 – 114Combined sources3
Helixi118 – 120Combined sources3
Beta strandi122 – 131Combined sources10
Helixi133 – 135Combined sources3
Beta strandi137 – 141Combined sources5
Beta strandi146 – 149Combined sources4
Beta strandi159 – 162Combined sources4
Beta strandi164 – 167Combined sources4
Beta strandi169 – 173Combined sources5
Beta strandi181 – 183Combined sources3
Turni187 – 190Combined sources4
Beta strandi192 – 194Combined sources3
Beta strandi197 – 199Combined sources3
Beta strandi202 – 204Combined sources3
Beta strandi207 – 211Combined sources5
Beta strandi217 – 221Combined sources5
Helixi222 – 224Combined sources3
Beta strandi225 – 227Combined sources3
Beta strandi234 – 236Combined sources3
Turni240 – 243Combined sources4
Helixi248 – 255Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10S41-261[»]
3J6Yelectron microscopy6.10S41-261[»]
3J77electron microscopy6.20S41-261[»]
3J78electron microscopy6.30S41-261[»]
4U3MX-ray3.00S4/s42-261[»]
4U3NX-ray3.20S4/s42-261[»]
4U3UX-ray2.90S4/s42-261[»]
4U4NX-ray3.10S4/s42-261[»]
4U4OX-ray3.60S4/s42-261[»]
4U4QX-ray3.00S4/s42-261[»]
4U4RX-ray2.80S4/s42-261[»]
4U4UX-ray3.00S4/s42-261[»]
4U4YX-ray3.20S4/s42-261[»]
4U4ZX-ray3.10S4/s42-261[»]
4U50X-ray3.20S4/s42-261[»]
4U51X-ray3.20S4/s42-261[»]
4U52X-ray3.00S4/s42-261[»]
4U53X-ray3.30S4/s42-261[»]
4U55X-ray3.20S4/s42-261[»]
4U56X-ray3.45S4/s42-261[»]
4U6FX-ray3.10S4/s42-261[»]
4V6Ielectron microscopy8.80AD1-261[»]
4V88X-ray3.00AE/CE1-261[»]
4V8Yelectron microscopy4.30AE1-261[»]
4V8Zelectron microscopy6.60AE1-261[»]
4V92electron microscopy3.70E2-261[»]
5DATX-ray3.15S4/s42-261[»]
5DC3X-ray3.25S4/s42-261[»]
5DGEX-ray3.45S4/s42-261[»]
5DGFX-ray3.30S4/s42-261[»]
5DGVX-ray3.10S4/s42-261[»]
5FCIX-ray3.40S4/s42-261[»]
5FCJX-ray3.10S4/s42-261[»]
5I4LX-ray3.10S4/s42-261[»]
5JUOelectron microscopy4.00BB1-261[»]
5JUPelectron microscopy3.50BB1-261[»]
5JUSelectron microscopy4.20BB1-261[»]
5JUTelectron microscopy4.00BB1-261[»]
5JUUelectron microscopy4.00BB1-261[»]
5LL6electron microscopy3.90S1-261[»]
5LYBX-ray3.25S4/s42-261[»]
5M1Jelectron microscopy3.30E22-261[»]
5MC6electron microscopy3.80S1-261[»]
5MEIX-ray3.50F/s42-261[»]
5TBWX-ray3.00F/s42-261[»]
5TGAX-ray3.30S4/s42-261[»]
5TGMX-ray3.50S4/s42-261[»]
5TZSelectron microscopy5.1051-261[»]
5WLCelectron microscopy3.80L41-261[»]
5WYJelectron microscopy8.70SF1-261[»]
5WYKelectron microscopy4.50SF1-261[»]
ProteinModelPortaliP0CX35.
SMRiP0CX35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 105S4 RNA-bindingAdd BLAST64

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000231325.
InParanoidiP0CX35.
KOiK02987.
OMAiRKDYKFP.
OrthoDBiEOG092C443N.

Family and domain databases

CDDicd00165. S4. 1 hit.
Gene3Di3.10.290.10. 2 hits.
HAMAPiMF_00485. Ribosomal_S4e. 1 hit.
InterProiView protein in InterPro
IPR032277. 40S_S4_C.
IPR005824. KOW.
IPR000876. Ribosomal_S4e.
IPR013845. Ribosomal_S4e_central_region.
IPR013843. Ribosomal_S4e_N.
IPR018199. Ribosomal_S4e_N_CS.
IPR002942. S4_RNA-bd.
IPR036986. S4_RNA-bd_sf.
PANTHERiPTHR11581. PTHR11581. 1 hit.
PfamiView protein in Pfam
PF16121. 40S_S4_C. 1 hit.
PF00467. KOW. 1 hit.
PF00900. Ribosomal_S4e. 1 hit.
PF08071. RS4NT. 1 hit.
PF01479. S4. 1 hit.
PIRSFiPIRSF002116. Ribosomal_S4. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002667. Ribosomal_S4e_central. 1 hit.
SMARTiView protein in SMART
SM00363. S4. 1 hit.
PROSITEiView protein in PROSITE
PS00528. RIBOSOMAL_S4E. 1 hit.
PS50889. S4. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN
60 70 80 90 100
RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDVI TLDATNENFR
110 120 130 140 150
LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP
160 170 180 190 200
DPNIKVNDTV KIDLASGKIT DFIKFDAGKL VYVTGGRNLG RIGTIVHKER
210 220 230 240 250
HDGGFDLVHI KDSLDNTFVT RLNNVFVIGE QGKPYISLPK GKGIKLSIAE
260
ERDRRRAQQG L
Length:261
Mass (Da):29,410
Last modified:June 28, 2011 - v1
Checksum:iF109594560F34734
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64293 Genomic DNA. Translation: AAA35011.1.
Z49645 Genomic DNA. Translation: CAA89678.1.
BK006943 Genomic DNA. Translation: DAA08930.1.
PIRiS20054.
RefSeqiNP_012073.1. NM_001179334.1.
NP_012679.3. NM_001181803.3.

Genome annotation databases

EnsemblFungiiYHR203C; YHR203C; YHR203C.
YJR145C; YJR145C; YJR145C.
GeneIDi853610.
856610.
KEGGisce:YHR203C.
sce:YJR145C.

Similar proteinsi

Entry informationi

Entry nameiRS4A_YEAST
AccessioniPrimary (citable) accession number: P0CX35
Secondary accession number(s): D3DLF2, P05753
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 22, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names