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Protein

40S ribosomal protein S4-A

Gene

RPS4A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. rRNA binding Source: UniProtKB-KW
  2. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31759-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_248935. Ribosomal scanning and start codon recognition.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257608. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S4-A
Alternative name(s):
RP5
S7
YS6
Gene namesi
Name:RPS4A
Synonyms:RPS7B
Ordered Locus Names:YJR145C
ORF Names:J2186
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR145c.
SGDiS000003906. RPS4A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 26126040S ribosomal protein S4-APRO_0000130843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321Phosphoserine1 Publication
Modified residuei115 – 1151Phosphothreonine1 Publication
Cross-linki168 – 168Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei247 – 2471Phosphoserine1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP0CX35.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX35. differential.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi33901. 171 interactions.
36637. 190 interactions.
IntActiP0CX35. 1 interaction.
MINTiMINT-8285663.

Structurei

Secondary structure

1
261
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni11 – 133Combined sources
Helixi16 – 183Combined sources
Beta strandi22 – 276Combined sources
Beta strandi33 – 364Combined sources
Helixi38 – 403Combined sources
Beta strandi41 – 433Combined sources
Helixi44 – 496Combined sources
Turni50 – 523Combined sources
Beta strandi56 – 583Combined sources
Helixi59 – 668Combined sources
Beta strandi70 – 723Combined sources
Beta strandi83 – 853Combined sources
Beta strandi89 – 924Combined sources
Helixi94 – 963Combined sources
Beta strandi97 – 1004Combined sources
Beta strandi101 – 1033Combined sources
Beta strandi105 – 1073Combined sources
Beta strandi112 – 1143Combined sources
Helixi118 – 1203Combined sources
Beta strandi122 – 13110Combined sources
Helixi133 – 1353Combined sources
Beta strandi137 – 1415Combined sources
Turni142 – 1443Combined sources
Beta strandi146 – 1494Combined sources
Beta strandi159 – 1624Combined sources
Beta strandi164 – 1674Combined sources
Beta strandi169 – 1735Combined sources
Beta strandi181 – 1833Combined sources
Turni187 – 1904Combined sources
Beta strandi192 – 1943Combined sources
Beta strandi197 – 1993Combined sources
Beta strandi202 – 2043Combined sources
Beta strandi207 – 2115Combined sources
Beta strandi217 – 2215Combined sources
Helixi222 – 2243Combined sources
Beta strandi225 – 2273Combined sources
Beta strandi230 – 2323Combined sources
Beta strandi234 – 2363Combined sources
Turni240 – 2434Combined sources
Helixi248 – 2558Combined sources
Turni256 – 2594Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10F1-261[»]
3J6Yelectron microscopy6.10F1-261[»]
3J77electron microscopy6.20E1-261[»]
3J78electron microscopy6.30E1-261[»]
3V88X-ray3.00E1-261[»]
4U3MX-ray3.00F/i2-261[»]
4U3NX-ray3.20F/i2-261[»]
4U3UX-ray2.90F/i2-261[»]
4U4NX-ray3.10F/i2-261[»]
4U4OX-ray3.60F/i2-261[»]
4U4QX-ray3.00F/i2-261[»]
4U4RX-ray2.80F/i2-261[»]
4U4UX-ray3.00F/i2-261[»]
4U4YX-ray3.20F/i2-261[»]
4U4ZX-ray3.10F/i2-261[»]
4U50X-ray3.20F/i2-261[»]
4U51X-ray3.20F/i2-261[»]
4U52X-ray3.00F/i2-261[»]
4U53X-ray3.30F/i2-261[»]
4U55X-ray3.20F/i2-261[»]
4U56X-ray3.45F/i2-261[»]
4U6FX-ray3.10F/i2-261[»]
4V6Ielectron microscopy-D1-261[»]
4V88X-ray3.00E1-261[»]
4V8Yelectron microscopy4.30E1-261[»]
4V8Zelectron microscopy6.60E1-261[»]
4V92electron microscopy3.70E2-261[»]
ProteinModelPortaliP0CX35.
SMRiP0CX35. Positions 2-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 10564S4 RNA-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the ribosomal protein S4e family.Curated
Contains 1 S4 RNA-binding domain.Curated

Phylogenomic databases

HOGENOMiHOG000231325.
InParanoidiP0CX35.
KOiK02987.
OrthoDBiEOG7JDR84.

Family and domain databases

HAMAPiMF_00485. Ribosomal_S4e.
InterProiIPR005824. KOW.
IPR000876. Ribosomal_S4e.
IPR013845. Ribosomal_S4e_central_region.
IPR013843. Ribosomal_S4e_N.
IPR018199. Ribosomal_S4e_N_CS.
IPR002942. S4_RNA-bd.
[Graphical view]
PANTHERiPTHR11581. PTHR11581. 1 hit.
PfamiPF00467. KOW. 1 hit.
PF00900. Ribosomal_S4e. 1 hit.
PF08071. RS4NT. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
PIRSFiPIRSF002116. Ribosomal_S4. 1 hit.
ProDomiPD002667. Ribosomal_S4e_central. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00739. KOW. 1 hit.
SM00363. S4. 1 hit.
[Graphical view]
PROSITEiPS00528. RIBOSOMAL_S4E. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX35-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN
60 70 80 90 100
RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDVI TLDATNENFR
110 120 130 140 150
LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP
160 170 180 190 200
DPNIKVNDTV KIDLASGKIT DFIKFDAGKL VYVTGGRNLG RIGTIVHKER
210 220 230 240 250
HDGGFDLVHI KDSLDNTFVT RLNNVFVIGE QGKPYISLPK GKGIKLSIAE
260
ERDRRRAQQG L
Length:261
Mass (Da):29,410
Last modified:June 28, 2011 - v1
Checksum:iF109594560F34734
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64293 Genomic DNA. Translation: AAA35011.1.
Z49645 Genomic DNA. Translation: CAA89678.1.
BK006943 Genomic DNA. Translation: DAA08930.1.
PIRiS20054.
RefSeqiNP_012073.1. NM_001179334.1.
NP_012679.3. NM_001181803.3.

Genome annotation databases

EnsemblFungiiYHR203C; YHR203C; YHR203C.
YJR145C; YJR145C; YJR145C.
GeneIDi853610.
856610.
KEGGisce:YHR203C.
sce:YJR145C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64293 Genomic DNA. Translation: AAA35011.1.
Z49645 Genomic DNA. Translation: CAA89678.1.
BK006943 Genomic DNA. Translation: DAA08930.1.
PIRiS20054.
RefSeqiNP_012073.1. NM_001179334.1.
NP_012679.3. NM_001181803.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10F1-261[»]
3J6Yelectron microscopy6.10F1-261[»]
3J77electron microscopy6.20E1-261[»]
3J78electron microscopy6.30E1-261[»]
3V88X-ray3.00E1-261[»]
4U3MX-ray3.00F/i2-261[»]
4U3NX-ray3.20F/i2-261[»]
4U3UX-ray2.90F/i2-261[»]
4U4NX-ray3.10F/i2-261[»]
4U4OX-ray3.60F/i2-261[»]
4U4QX-ray3.00F/i2-261[»]
4U4RX-ray2.80F/i2-261[»]
4U4UX-ray3.00F/i2-261[»]
4U4YX-ray3.20F/i2-261[»]
4U4ZX-ray3.10F/i2-261[»]
4U50X-ray3.20F/i2-261[»]
4U51X-ray3.20F/i2-261[»]
4U52X-ray3.00F/i2-261[»]
4U53X-ray3.30F/i2-261[»]
4U55X-ray3.20F/i2-261[»]
4U56X-ray3.45F/i2-261[»]
4U6FX-ray3.10F/i2-261[»]
4V6Ielectron microscopy-D1-261[»]
4V88X-ray3.00E1-261[»]
4V8Yelectron microscopy4.30E1-261[»]
4V8Zelectron microscopy6.60E1-261[»]
4V92electron microscopy3.70E2-261[»]
ProteinModelPortaliP0CX35.
SMRiP0CX35. Positions 2-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33901. 171 interactions.
36637. 190 interactions.
IntActiP0CX35. 1 interaction.
MINTiMINT-8285663.

Proteomic databases

MaxQBiP0CX35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR203C; YHR203C; YHR203C.
YJR145C; YJR145C; YJR145C.
GeneIDi853610.
856610.
KEGGisce:YHR203C.
sce:YJR145C.

Organism-specific databases

CYGDiYJR145c.
SGDiS000003906. RPS4A.

Phylogenomic databases

HOGENOMiHOG000231325.
InParanoidiP0CX35.
KOiK02987.
OrthoDBiEOG7JDR84.

Enzyme and pathway databases

BioCyciYEAST:G3O-31759-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_248935. Ribosomal scanning and start codon recognition.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257608. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

NextBioi974454.

Gene expression databases

ExpressionAtlasiP0CX35. differential.

Family and domain databases

HAMAPiMF_00485. Ribosomal_S4e.
InterProiIPR005824. KOW.
IPR000876. Ribosomal_S4e.
IPR013845. Ribosomal_S4e_central_region.
IPR013843. Ribosomal_S4e_N.
IPR018199. Ribosomal_S4e_N_CS.
IPR002942. S4_RNA-bd.
[Graphical view]
PANTHERiPTHR11581. PTHR11581. 1 hit.
PfamiPF00467. KOW. 1 hit.
PF00900. Ribosomal_S4e. 1 hit.
PF08071. RS4NT. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
PIRSFiPIRSF002116. Ribosomal_S4. 1 hit.
ProDomiPD002667. Ribosomal_S4e_central. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00739. KOW. 1 hit.
SM00363. S4. 1 hit.
[Graphical view]
PROSITEiPS00528. RIBOSOMAL_S4E. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast ribosomal protein S7 and its genes."
    Synetos D., Dabeva M.D., Warner J.R.
    J. Biol. Chem. 267:3008-3013(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast."
    Otaka E., Higo K., Osawa S.
    Biochemistry 21:4545-4550(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-29.
  5. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  6. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-168.
    Strain: SUB592.
  10. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-115, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRS4A_YEAST
AccessioniPrimary (citable) accession number: P0CX35
Secondary accession number(s): D3DLF2, P05753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: February 4, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S4 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.