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Protein

40S ribosomal protein S4-A

Gene

RPS4A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • rRNA binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • translation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31759-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S4-A
Alternative name(s):
RP5
S7
YS6
Gene namesi
Name:RPS4A
Synonyms:RPS7B
Ordered Locus Names:YJR145C
ORF Names:J2186
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

SGDiS000003906. RPS4A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 26126040S ribosomal protein S4-APRO_0000130843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321PhosphoserineCombined sources
Cross-linki62 – 62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei115 – 1151PhosphothreonineCombined sources
Cross-linki134 – 134Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki161 – 161Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki168 – 168Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources1 Publication
Cross-linki174 – 174Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki179 – 179Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki211 – 211Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki233 – 233Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei247 – 2471PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP0CX35.
TopDownProteomicsiP0CX35.

PTM databases

iPTMnetiP0CX35.

Expressioni

Gene expression databases

ExpressionAtlasiP0CX35. differential.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi33901. 116 interactions.
36637. 130 interactions.
IntActiP0CX35. 7 interactions.
MINTiMINT-8285663.

Structurei

Secondary structure

1
261
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni11 – 133Combined sources
Helixi16 – 183Combined sources
Beta strandi22 – 276Combined sources
Beta strandi33 – 364Combined sources
Helixi38 – 403Combined sources
Beta strandi41 – 433Combined sources
Helixi44 – 496Combined sources
Turni50 – 523Combined sources
Beta strandi56 – 583Combined sources
Helixi59 – 668Combined sources
Beta strandi70 – 723Combined sources
Beta strandi83 – 853Combined sources
Beta strandi89 – 924Combined sources
Helixi94 – 963Combined sources
Beta strandi97 – 1004Combined sources
Beta strandi101 – 1033Combined sources
Beta strandi105 – 1073Combined sources
Beta strandi112 – 1143Combined sources
Helixi118 – 1203Combined sources
Beta strandi122 – 13110Combined sources
Helixi133 – 1353Combined sources
Beta strandi137 – 1415Combined sources
Turni142 – 1443Combined sources
Beta strandi146 – 1494Combined sources
Beta strandi159 – 1624Combined sources
Beta strandi164 – 1674Combined sources
Beta strandi169 – 1735Combined sources
Beta strandi181 – 1833Combined sources
Turni187 – 1904Combined sources
Beta strandi192 – 1943Combined sources
Beta strandi197 – 1993Combined sources
Beta strandi202 – 2043Combined sources
Beta strandi207 – 2115Combined sources
Beta strandi217 – 2215Combined sources
Helixi222 – 2243Combined sources
Beta strandi225 – 2273Combined sources
Beta strandi230 – 2323Combined sources
Beta strandi234 – 2363Combined sources
Turni240 – 2434Combined sources
Helixi248 – 2558Combined sources
Turni256 – 2594Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10S41-261[»]
3J6Yelectron microscopy6.10S41-261[»]
3J77electron microscopy6.20S41-261[»]
3J78electron microscopy6.30S41-261[»]
3V88X-ray3.00E1-261[»]
4U3MX-ray3.00S4/s42-261[»]
4U3NX-ray3.20S4/s42-261[»]
4U3UX-ray2.90S4/s42-261[»]
4U4NX-ray3.10S4/s42-261[»]
4U4OX-ray3.60S4/s42-261[»]
4U4QX-ray3.00S4/s42-261[»]
4U4RX-ray2.80S4/s42-261[»]
4U4UX-ray3.00S4/s42-261[»]
4U4YX-ray3.20S4/s42-261[»]
4U4ZX-ray3.10S4/s42-261[»]
4U50X-ray3.20S4/s42-261[»]
4U51X-ray3.20S4/s42-261[»]
4U52X-ray3.00S4/s42-261[»]
4U53X-ray3.30S4/s42-261[»]
4U55X-ray3.20S4/s42-261[»]
4U56X-ray3.45S4/s42-261[»]
4U6FX-ray3.10S4/s42-261[»]
4V6Ielectron microscopy8.80AD1-261[»]
4V88X-ray3.00AE/CE1-261[»]
4V8Yelectron microscopy4.30AE1-261[»]
4V8Zelectron microscopy6.60AE1-261[»]
4V92electron microscopy3.70E2-261[»]
5DC3X-ray3.25S4/s42-261[»]
5FCIX-ray3.40S4/s42-261[»]
5FCJX-ray3.10S4/s42-261[»]
5I4LX-ray3.10S4/s42-261[»]
ProteinModelPortaliP0CX35.
SMRiP0CX35. Positions 2-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 10564S4 RNA-bindingAdd
BLAST

Sequence similaritiesi

Belongs to the ribosomal protein S4e family.Curated
Contains 1 S4 RNA-binding domain.Curated

Phylogenomic databases

HOGENOMiHOG000231325.
InParanoidiP0CX35.
KOiK02987.
OrthoDBiEOG092C443N.

Family and domain databases

HAMAPiMF_00485. Ribosomal_S4e. 1 hit.
InterProiIPR032277. 40S_S4_C.
IPR005824. KOW.
IPR000876. Ribosomal_S4e.
IPR013845. Ribosomal_S4e_central_region.
IPR013843. Ribosomal_S4e_N.
IPR018199. Ribosomal_S4e_N_CS.
IPR002942. S4_RNA-bd.
[Graphical view]
PANTHERiPTHR11581. PTHR11581. 1 hit.
PfamiPF16121. 40S_S4_C. 1 hit.
PF00467. KOW. 1 hit.
PF00900. Ribosomal_S4e. 1 hit.
PF08071. RS4NT. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
PIRSFiPIRSF002116. Ribosomal_S4. 1 hit.
ProDomiPD002667. Ribosomal_S4e_central. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
PROSITEiPS00528. RIBOSOMAL_S4E. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN
60 70 80 90 100
RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDVI TLDATNENFR
110 120 130 140 150
LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP
160 170 180 190 200
DPNIKVNDTV KIDLASGKIT DFIKFDAGKL VYVTGGRNLG RIGTIVHKER
210 220 230 240 250
HDGGFDLVHI KDSLDNTFVT RLNNVFVIGE QGKPYISLPK GKGIKLSIAE
260
ERDRRRAQQG L
Length:261
Mass (Da):29,410
Last modified:June 28, 2011 - v1
Checksum:iF109594560F34734
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64293 Genomic DNA. Translation: AAA35011.1.
Z49645 Genomic DNA. Translation: CAA89678.1.
BK006943 Genomic DNA. Translation: DAA08930.1.
PIRiS20054.
RefSeqiNP_012073.1. NM_001179334.1.
NP_012679.3. NM_001181803.3.

Genome annotation databases

EnsemblFungiiYHR203C; YHR203C; YHR203C.
YJR145C; YJR145C; YJR145C.
GeneIDi853610.
856610.
KEGGisce:YHR203C.
sce:YJR145C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64293 Genomic DNA. Translation: AAA35011.1.
Z49645 Genomic DNA. Translation: CAA89678.1.
BK006943 Genomic DNA. Translation: DAA08930.1.
PIRiS20054.
RefSeqiNP_012073.1. NM_001179334.1.
NP_012679.3. NM_001181803.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10S41-261[»]
3J6Yelectron microscopy6.10S41-261[»]
3J77electron microscopy6.20S41-261[»]
3J78electron microscopy6.30S41-261[»]
3V88X-ray3.00E1-261[»]
4U3MX-ray3.00S4/s42-261[»]
4U3NX-ray3.20S4/s42-261[»]
4U3UX-ray2.90S4/s42-261[»]
4U4NX-ray3.10S4/s42-261[»]
4U4OX-ray3.60S4/s42-261[»]
4U4QX-ray3.00S4/s42-261[»]
4U4RX-ray2.80S4/s42-261[»]
4U4UX-ray3.00S4/s42-261[»]
4U4YX-ray3.20S4/s42-261[»]
4U4ZX-ray3.10S4/s42-261[»]
4U50X-ray3.20S4/s42-261[»]
4U51X-ray3.20S4/s42-261[»]
4U52X-ray3.00S4/s42-261[»]
4U53X-ray3.30S4/s42-261[»]
4U55X-ray3.20S4/s42-261[»]
4U56X-ray3.45S4/s42-261[»]
4U6FX-ray3.10S4/s42-261[»]
4V6Ielectron microscopy8.80AD1-261[»]
4V88X-ray3.00AE/CE1-261[»]
4V8Yelectron microscopy4.30AE1-261[»]
4V8Zelectron microscopy6.60AE1-261[»]
4V92electron microscopy3.70E2-261[»]
5DC3X-ray3.25S4/s42-261[»]
5FCIX-ray3.40S4/s42-261[»]
5FCJX-ray3.10S4/s42-261[»]
5I4LX-ray3.10S4/s42-261[»]
ProteinModelPortaliP0CX35.
SMRiP0CX35. Positions 2-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33901. 116 interactions.
36637. 130 interactions.
IntActiP0CX35. 7 interactions.
MINTiMINT-8285663.

PTM databases

iPTMnetiP0CX35.

Proteomic databases

MaxQBiP0CX35.
TopDownProteomicsiP0CX35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR203C; YHR203C; YHR203C.
YJR145C; YJR145C; YJR145C.
GeneIDi853610.
856610.
KEGGisce:YHR203C.
sce:YJR145C.

Organism-specific databases

SGDiS000003906. RPS4A.

Phylogenomic databases

HOGENOMiHOG000231325.
InParanoidiP0CX35.
KOiK02987.
OrthoDBiEOG092C443N.

Enzyme and pathway databases

BioCyciYEAST:G3O-31759-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiP0CX35.

Gene expression databases

ExpressionAtlasiP0CX35. differential.

Family and domain databases

HAMAPiMF_00485. Ribosomal_S4e. 1 hit.
InterProiIPR032277. 40S_S4_C.
IPR005824. KOW.
IPR000876. Ribosomal_S4e.
IPR013845. Ribosomal_S4e_central_region.
IPR013843. Ribosomal_S4e_N.
IPR018199. Ribosomal_S4e_N_CS.
IPR002942. S4_RNA-bd.
[Graphical view]
PANTHERiPTHR11581. PTHR11581. 1 hit.
PfamiPF16121. 40S_S4_C. 1 hit.
PF00467. KOW. 1 hit.
PF00900. Ribosomal_S4e. 1 hit.
PF08071. RS4NT. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
PIRSFiPIRSF002116. Ribosomal_S4. 1 hit.
ProDomiPD002667. Ribosomal_S4e_central. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
PROSITEiPS00528. RIBOSOMAL_S4E. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRS4A_YEAST
AccessioniPrimary (citable) accession number: P0CX35
Secondary accession number(s): D3DLF2, P05753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: September 7, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S4 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.