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P0CX35

- RS4A_YEAST

UniProt

P0CX35 - RS4A_YEAST

Protein

40S ribosomal protein S4-A

Gene

RPS4A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. rRNA binding Source: UniProtKB-KW
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31759-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S4-A
    Alternative name(s):
    RP5
    S7
    YS6
    Gene namesi
    Name:RPS4A
    Synonyms:RPS7B
    Ordered Locus Names:YJR145C
    ORF Names:J2186
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR145c.
    SGDiS000003906. RPS4A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 26126040S ribosomal protein S4-APRO_0000130843Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei32 – 321Phosphoserine1 Publication
    Modified residuei115 – 1151Phosphothreonine1 Publication
    Cross-linki168 – 168Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Modified residuei247 – 2471Phosphoserine1 Publication

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP0CX35.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi33901. 169 interactions.
    36637. 188 interactions.
    IntActiP0CX35. 1 interaction.
    MINTiMINT-8285663.

    Structurei

    Secondary structure

    1
    261
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni11 – 133
    Helixi16 – 183
    Beta strandi22 – 276
    Beta strandi33 – 364
    Helixi38 – 403
    Helixi44 – 507
    Beta strandi56 – 583
    Helixi59 – 657
    Turni66 – 683
    Beta strandi70 – 723
    Beta strandi89 – 924
    Turni93 – 964
    Beta strandi97 – 1004
    Beta strandi105 – 1073
    Helixi118 – 1203
    Beta strandi126 – 1316
    Helixi133 – 1353
    Beta strandi137 – 1415
    Turni142 – 1443
    Beta strandi145 – 1495
    Beta strandi159 – 1624
    Beta strandi164 – 1674
    Beta strandi169 – 1735
    Beta strandi181 – 1833
    Turni187 – 1904
    Beta strandi197 – 1993
    Beta strandi207 – 2115
    Beta strandi217 – 2215
    Helixi222 – 2243
    Beta strandi225 – 2273
    Beta strandi230 – 2323
    Beta strandi233 – 2364
    Turni240 – 2434
    Helixi248 – 2558
    Turni256 – 2594

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VW9electron microscopy6.10F1-261[»]
    1VWVelectron microscopy6.10F1-261[»]
    3IZBelectron microscopy-D1-261[»]
    3U5CX-ray3.00E1-261[»]
    3U5GX-ray3.00E1-261[»]
    4BYLelectron microscopy4.30E1-261[»]
    4BYTelectron microscopy6.60E1-261[»]
    4CUYelectron microscopy3.70E2-261[»]
    ProteinModelPortaliP0CX35.
    SMRiP0CX35. Positions 2-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 10564S4 RNA-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ribosomal protein S4e family.Curated
    Contains 1 S4 RNA-binding domain.Curated

    Phylogenomic databases

    HOGENOMiHOG000231325.
    KOiK02987.
    OrthoDBiEOG7JDR84.

    Family and domain databases

    HAMAPiMF_00485. Ribosomal_S4e.
    InterProiIPR005824. KOW.
    IPR000876. Ribosomal_S4e.
    IPR013845. Ribosomal_S4e_central_region.
    IPR013843. Ribosomal_S4e_N.
    IPR018199. Ribosomal_S4e_N_CS.
    IPR002942. S4_RNA-bd.
    [Graphical view]
    PANTHERiPTHR11581. PTHR11581. 1 hit.
    PfamiPF00467. KOW. 1 hit.
    PF00900. Ribosomal_S4e. 1 hit.
    PF08071. RS4NT. 1 hit.
    PF01479. S4. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002116. Ribosomal_S4. 1 hit.
    ProDomiPD002667. Ribosomal_S4e_central. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00739. KOW. 1 hit.
    SM00363. S4. 1 hit.
    [Graphical view]
    PROSITEiPS00528. RIBOSOMAL_S4E. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CX35-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN    50
    RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDVI TLDATNENFR 100
    LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP 150
    DPNIKVNDTV KIDLASGKIT DFIKFDAGKL VYVTGGRNLG RIGTIVHKER 200
    HDGGFDLVHI KDSLDNTFVT RLNNVFVIGE QGKPYISLPK GKGIKLSIAE 250
    ERDRRRAQQG L 261
    Length:261
    Mass (Da):29,410
    Last modified:June 28, 2011 - v1
    Checksum:iF109594560F34734
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64293 Genomic DNA. Translation: AAA35011.1.
    Z49645 Genomic DNA. Translation: CAA89678.1.
    BK006943 Genomic DNA. Translation: DAA08930.1.
    PIRiS20054.
    RefSeqiNP_012073.1. NM_001179334.1.
    NP_012679.3. NM_001181803.3.

    Genome annotation databases

    EnsemblFungiiYHR203C; YHR203C; YHR203C.
    YJR145C; YJR145C; YJR145C.
    GeneIDi853610.
    856610.
    KEGGisce:YHR203C.
    sce:YJR145C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64293 Genomic DNA. Translation: AAA35011.1 .
    Z49645 Genomic DNA. Translation: CAA89678.1 .
    BK006943 Genomic DNA. Translation: DAA08930.1 .
    PIRi S20054.
    RefSeqi NP_012073.1. NM_001179334.1.
    NP_012679.3. NM_001181803.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VW9 electron microscopy 6.10 F 1-261 [» ]
    1VWV electron microscopy 6.10 F 1-261 [» ]
    3IZB electron microscopy - D 1-261 [» ]
    3U5C X-ray 3.00 E 1-261 [» ]
    3U5G X-ray 3.00 E 1-261 [» ]
    4BYL electron microscopy 4.30 E 1-261 [» ]
    4BYT electron microscopy 6.60 E 1-261 [» ]
    4CUY electron microscopy 3.70 E 2-261 [» ]
    ProteinModelPortali P0CX35.
    SMRi P0CX35. Positions 2-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33901. 169 interactions.
    36637. 188 interactions.
    IntActi P0CX35. 1 interaction.
    MINTi MINT-8285663.

    Proteomic databases

    MaxQBi P0CX35.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHR203C ; YHR203C ; YHR203C .
    YJR145C ; YJR145C ; YJR145C .
    GeneIDi 853610.
    856610.
    KEGGi sce:YHR203C.
    sce:YJR145C.

    Organism-specific databases

    CYGDi YJR145c.
    SGDi S000003906. RPS4A.

    Phylogenomic databases

    HOGENOMi HOG000231325.
    KOi K02987.
    OrthoDBi EOG7JDR84.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31759-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    NextBioi 974454.

    Family and domain databases

    HAMAPi MF_00485. Ribosomal_S4e.
    InterProi IPR005824. KOW.
    IPR000876. Ribosomal_S4e.
    IPR013845. Ribosomal_S4e_central_region.
    IPR013843. Ribosomal_S4e_N.
    IPR018199. Ribosomal_S4e_N_CS.
    IPR002942. S4_RNA-bd.
    [Graphical view ]
    PANTHERi PTHR11581. PTHR11581. 1 hit.
    Pfami PF00467. KOW. 1 hit.
    PF00900. Ribosomal_S4e. 1 hit.
    PF08071. RS4NT. 1 hit.
    PF01479. S4. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002116. Ribosomal_S4. 1 hit.
    ProDomi PD002667. Ribosomal_S4e_central. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00739. KOW. 1 hit.
    SM00363. S4. 1 hit.
    [Graphical view ]
    PROSITEi PS00528. RIBOSOMAL_S4E. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast ribosomal protein S7 and its genes."
      Synetos D., Dabeva M.D., Warner J.R.
      J. Biol. Chem. 267:3008-3013(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast."
      Otaka E., Higo K., Osawa S.
      Biochemistry 21:4545-4550(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-29.
    5. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    6. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-168.
      Strain: SUB592.
    10. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    12. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-115, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRS4A_YEAST
    AccessioniPrimary (citable) accession number: P0CX35
    Secondary accession number(s): D3DLF2, P05753
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 573 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for S4 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3