Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0CX33

- RS30A_YEAST

UniProt

P0CX33 - RS30A_YEAST

Protein

40S ribosomal protein S30-A

Gene

RPS30A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32382-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S30-A
    Gene namesi
    Name:RPS30A
    Ordered Locus Names:YLR287C-A
    ORF Names:L8003.23, YLR287BC
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR287c-a.
    SGDiS000004278. RPS30A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasmic stress granule Source: SGD
    2. cytosolic small ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 636240S ribosomal protein S30-APRO_0000174009Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei16 – 161Phosphoserine1 Publication
    Modified residuei48 – 481Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP0CX33.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi31553. 114 interactions.
    34577. 54 interactions.

    Structurei

    Secondary structure

    1
    63
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni9 – 124
    Helixi13 – 175
    Helixi33 – 4412
    Beta strandi51 – 533

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VW9electron microscopy6.10f1-63[»]
    1VWVelectron microscopy6.10f1-63[»]
    3IZBelectron microscopy-Z1-63[»]
    3J16electron microscopy-E1-63[»]
    3U5CX-ray3.00e1-63[»]
    3U5GX-ray3.00e1-63[»]
    4BYLelectron microscopy4.3041-63[»]
    4BYTelectron microscopy6.6041-63[»]
    4CUYelectron microscopy3.70e7-61[»]
    ProteinModelPortaliP0CX33.
    SMRiP0CX33. Positions 2-63.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein S30e family.Curated

    Phylogenomic databases

    KOiK02983.
    OrthoDBiEOG71CG0N.

    Family and domain databases

    InterProiIPR006846. Ribosomal_S30.
    [Graphical view]
    PfamiPF04758. Ribosomal_S30. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CX33-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKVHGSLAR AGKVKSQTPK VEKTEKPKKP KGRAYKRLLY TRRFVNVTLV   50
    NGKRRMNPGP SVQ 63
    Length:63
    Mass (Da):7,118
    Last modified:June 28, 2011 - v1
    Checksum:i658C4C1D8D9F88CB
    GO

    Mass spectrometryi

    Molecular mass is 6987±3.4 Da from positions 2 - 63. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48700 Genomic DNA. Translation: AAC49317.1.
    U48699 mRNA. Translation: AAC49316.1.
    U17243 Genomic DNA. Translation: AAB67333.1.
    BK006945 Genomic DNA. Translation: DAA09599.1.
    PIRiS67074.
    RefSeqiNP_013390.1. NM_001182175.1.
    NP_014825.3. NM_001183601.3.

    Genome annotation databases

    EnsemblFungiiYLR287C-A; YLR287C-A; YLR287C-A.
    YOR182C; YOR182C; YOR182C.
    GeneIDi850994.
    854354.
    KEGGisce:YLR287C-A.
    sce:YOR182C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48700 Genomic DNA. Translation: AAC49317.1 .
    U48699 mRNA. Translation: AAC49316.1 .
    U17243 Genomic DNA. Translation: AAB67333.1 .
    BK006945 Genomic DNA. Translation: DAA09599.1 .
    PIRi S67074.
    RefSeqi NP_013390.1. NM_001182175.1.
    NP_014825.3. NM_001183601.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VW9 electron microscopy 6.10 f 1-63 [» ]
    1VWV electron microscopy 6.10 f 1-63 [» ]
    3IZB electron microscopy - Z 1-63 [» ]
    3J16 electron microscopy - E 1-63 [» ]
    3U5C X-ray 3.00 e 1-63 [» ]
    3U5G X-ray 3.00 e 1-63 [» ]
    4BYL electron microscopy 4.30 4 1-63 [» ]
    4BYT electron microscopy 6.60 4 1-63 [» ]
    4CUY electron microscopy 3.70 e 7-61 [» ]
    ProteinModelPortali P0CX33.
    SMRi P0CX33. Positions 2-63.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31553. 114 interactions.
    34577. 54 interactions.

    Proteomic databases

    MaxQBi P0CX33.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR287C-A ; YLR287C-A ; YLR287C-A .
    YOR182C ; YOR182C ; YOR182C .
    GeneIDi 850994.
    854354.
    KEGGi sce:YLR287C-A.
    sce:YOR182C.

    Organism-specific databases

    CYGDi YLR287c-a.
    SGDi S000004278. RPS30A.

    Phylogenomic databases

    KOi K02983.
    OrthoDBi EOG71CG0N.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32382-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    NextBioi 967525.

    Family and domain databases

    InterProi IPR006846. Ribosomal_S30.
    [Graphical view ]
    Pfami PF04758. Ribosomal_S30. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast homolog of mammalian ribosomal protein S30 is expressed from a duplicated gene without a ubiquitin-like protein fusion sequence. Evolutionary implications."
      Baker R.T., Williamson N.A., Wettenhall R.E.H.
      J. Biol. Chem. 271:13549-13555(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE, MASS SPECTROMETRY.
      Strain: YRB141.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    5. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND THR-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRS30A_YEAST
    AccessioniPrimary (citable) accession number: P0CX33
    Secondary accession number(s): D6VYT3, Q12087
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 37600 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for S30 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3