P0CX33 (RS30A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S30-A

Gene names

Name:	RPS30A
Ordered Locus Names:	YLR287C-A
ORF Names:	L8003.23, YLR287BC

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	63 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm Ref.6.
Miscellaneous	Present with 37600 molecules/cell in log phase SD medium. There are 2 genes for S30 in yeast.
Sequence similarities	Belongs to the ribosomal protein S30e family.
Mass spectrometry	Molecular mass is 6987±3.4 Da from positions 2 - 63. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Non-traceable author statement Ref.4. Source: SGD
Cellular_component	cytoplasmic stress granule Inferred from direct assay PubMed 19470581. Source: SGD cytosolic small ribosomal subunit Non-traceable author statement Ref.4. Source: SGD
Molecular_function	structural constituent of ribosome Non-traceable author statement Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5

Chain

2 – 63

62

40S ribosomal protein S30-A

PRO_0000174009

Amino acid modifications

Modified residue

7

1

Phosphoserine Ref.9

Modified residue

16

1

Phosphoserine Ref.8

Modified residue

41

1

Phosphothreonine Ref.8

Modified residue

48

1

Phosphothreonine Ref.8

Secondary structure

1

.

.

.

.

.

.

.

.

63

Helix Strand Turn

Details...

Turn

9 – 12

4

Helix

13 – 17

5

Helix

33 – 44

12

Beta strand

51 – 53

3

Sequences

	Sequence		Length	Mass (Da)
	P0CX33 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 658C4C1D8D9F88CB	FASTA	63	7,118
	10 20 30 40 50 60 MAKVHGSLAR AGKVKSQTPK VEKTEKPKKP KGRAYKRLLY TRRFVNVTLV NGKRRMNPGP SVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast homolog of mammalian ribosomal protein S30 is expressed from a duplicated gene without a ubiquitin-like protein fusion sequence. Evolutionary implications." Baker R.T., Williamson N.A., Wettenhall R.E.H. J. Biol. Chem. 271:13549-13555(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE, MASS SPECTROMETRY. Strain: YRB141.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; THR-41 AND THR-48, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U48700 Genomic DNA. Translation: AAC49317.1.
U48699 mRNA. Translation: AAC49316.1.
U17243 Genomic DNA. Translation: AAB67333.1.
BK006945 Genomic DNA. Translation: DAA09599.1.

PIR

S67074.

RefSeq

NP_013390.1. NM_001182175.1.
NP_014825.3. NM_001183601.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IZB	electron microscopy	-	Z	1-63	[»]
3J16	electron microscopy	-	E	1-63	[»]
3U5C	X-ray	3.00	e	1-63	[»]
3U5G	X-ray	3.00	e	1-63	[»]

ProteinModelPortal

P0CX33.

SMR

P0CX33. Positions 2-63.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YLR287C-A; YLR287C-A; YLR287C-A.
YOR182C; YOR182C; YOR182C.

GeneID

850994.
854354.

KEGG

sce:YLR287C-A.
sce:YOR182C.
sce:YOR184W.

Organism-specific databases

CYGD

YLR287c-a.

SGD

S000004278. RPS30A.

Phylogenomic databases

KO

K00831.
K02983.

Gene expression databases

ArrayExpress

P0CX33.

GermOnline

YLR287C-A. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR006846. Ribosomal_S30.
[Graphical view]

Pfam

PF04758. Ribosomal_S30. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

967525.

Entry information

Entry name

RS30A_YEAST

Accession

Primary (citable) accession number: P0CX33
Secondary accession number(s): D6VYT3, Q12087

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program