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Protein

40S ribosomal protein S30-A

Gene

RPS30A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32382-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_335938. Ribosomal scanning and start codon recognition.
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_351622. Formation of the ternary complex, and subsequently, the 43S complex.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S30-A
Gene namesi
Name:RPS30A
Ordered Locus Names:YLR287C-A
ORF Names:L8003.23, YLR287BC
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XII

Organism-specific databases

CYGDiYLR287c-a.
SGDiS000004278. RPS30A.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic stress granule Source: SGD
  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 636240S ribosomal protein S30-APRO_0000174009Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei16 – 161Phosphoserine1 Publication
Modified residuei48 – 481Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP0CX33.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi31553. 112 interactions.
34577. 52 interactions.

Structurei

Secondary structure

1
63
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni9 – 124Combined sources
Helixi13 – 175Combined sources
Helixi33 – 4311Combined sources
Turni44 – 463Combined sources
Beta strandi51 – 533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J16electron microscopy-E1-63[»]
3J6Xelectron microscopy6.10301-63[»]
3J6Yelectron microscopy6.10301-63[»]
3J77electron microscopy6.20301-63[»]
3J78electron microscopy6.30301-63[»]
3V88X-ray3.00e1-63[»]
4U3MX-ray3.00E02-61[»]
e02-63[»]
4U3NX-ray3.20E02-61[»]
e02-63[»]
4U3UX-ray2.90E02-61[»]
e02-63[»]
4U4NX-ray3.10E02-61[»]
e02-63[»]
4U4OX-ray3.60E02-61[»]
e02-63[»]
4U4QX-ray3.00E02-61[»]
e02-63[»]
4U4RX-ray2.80E02-61[»]
e02-63[»]
4U4UX-ray3.00E02-61[»]
e02-63[»]
4U4YX-ray3.20E02-61[»]
e02-63[»]
4U4ZX-ray3.10E02-61[»]
e02-63[»]
4U50X-ray3.20E02-61[»]
e02-63[»]
4U51X-ray3.20E02-61[»]
e02-63[»]
4U52X-ray3.00E0/e02-61[»]
4U53X-ray3.30E02-61[»]
e02-63[»]
4U55X-ray3.20E02-61[»]
e02-63[»]
4U56X-ray3.45E02-61[»]
e02-63[»]
4U6FX-ray3.10E02-61[»]
e02-63[»]
4V6Ielectron microscopy8.80AZ1-63[»]
4V88X-ray3.00Ae/Ce1-63[»]
4V8Yelectron microscopy4.30A41-63[»]
4V8Zelectron microscopy6.60A41-63[»]
4V92electron microscopy3.70e7-61[»]
ProteinModelPortaliP0CX33.
SMRiP0CX33. Positions 8-61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S30e family.Curated

Phylogenomic databases

InParanoidiP0CX33.
KOiK02983.
OrthoDBiEOG71CG0N.

Family and domain databases

InterProiIPR006846. Ribosomal_S30.
[Graphical view]
PfamiPF04758. Ribosomal_S30. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CX33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVHGSLAR AGKVKSQTPK VEKTEKPKKP KGRAYKRLLY TRRFVNVTLV
60
NGKRRMNPGP SVQ
Length:63
Mass (Da):7,118
Last modified:June 28, 2011 - v1
Checksum:i658C4C1D8D9F88CB
GO

Mass spectrometryi

Molecular mass is 6987±3.4 Da from positions 2 - 63. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48700 Genomic DNA. Translation: AAC49317.1.
U48699 mRNA. Translation: AAC49316.1.
U17243 Genomic DNA. Translation: AAB67333.1.
BK006945 Genomic DNA. Translation: DAA09599.1.
PIRiS67074.
RefSeqiNP_013390.1. NM_001182175.1.
NP_014825.3. NM_001183601.3.

Genome annotation databases

EnsemblFungiiYLR287C-A; YLR287C-A; YLR287C-A.
YOR182C; YOR182C; YOR182C.
GeneIDi850994.
854354.
KEGGisce:YLR287C-A.
sce:YOR182C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48700 Genomic DNA. Translation: AAC49317.1.
U48699 mRNA. Translation: AAC49316.1.
U17243 Genomic DNA. Translation: AAB67333.1.
BK006945 Genomic DNA. Translation: DAA09599.1.
PIRiS67074.
RefSeqiNP_013390.1. NM_001182175.1.
NP_014825.3. NM_001183601.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J16electron microscopy-E1-63[»]
3J6Xelectron microscopy6.10301-63[»]
3J6Yelectron microscopy6.10301-63[»]
3J77electron microscopy6.20301-63[»]
3J78electron microscopy6.30301-63[»]
3V88X-ray3.00e1-63[»]
4U3MX-ray3.00E02-61[»]
e02-63[»]
4U3NX-ray3.20E02-61[»]
e02-63[»]
4U3UX-ray2.90E02-61[»]
e02-63[»]
4U4NX-ray3.10E02-61[»]
e02-63[»]
4U4OX-ray3.60E02-61[»]
e02-63[»]
4U4QX-ray3.00E02-61[»]
e02-63[»]
4U4RX-ray2.80E02-61[»]
e02-63[»]
4U4UX-ray3.00E02-61[»]
e02-63[»]
4U4YX-ray3.20E02-61[»]
e02-63[»]
4U4ZX-ray3.10E02-61[»]
e02-63[»]
4U50X-ray3.20E02-61[»]
e02-63[»]
4U51X-ray3.20E02-61[»]
e02-63[»]
4U52X-ray3.00E0/e02-61[»]
4U53X-ray3.30E02-61[»]
e02-63[»]
4U55X-ray3.20E02-61[»]
e02-63[»]
4U56X-ray3.45E02-61[»]
e02-63[»]
4U6FX-ray3.10E02-61[»]
e02-63[»]
4V6Ielectron microscopy8.80AZ1-63[»]
4V88X-ray3.00Ae/Ce1-63[»]
4V8Yelectron microscopy4.30A41-63[»]
4V8Zelectron microscopy6.60A41-63[»]
4V92electron microscopy3.70e7-61[»]
ProteinModelPortaliP0CX33.
SMRiP0CX33. Positions 8-61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31553. 112 interactions.
34577. 52 interactions.

Proteomic databases

MaxQBiP0CX33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR287C-A; YLR287C-A; YLR287C-A.
YOR182C; YOR182C; YOR182C.
GeneIDi850994.
854354.
KEGGisce:YLR287C-A.
sce:YOR182C.

Organism-specific databases

CYGDiYLR287c-a.
SGDiS000004278. RPS30A.

Phylogenomic databases

InParanoidiP0CX33.
KOiK02983.
OrthoDBiEOG71CG0N.

Enzyme and pathway databases

BioCyciYEAST:G3O-32382-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_335938. Ribosomal scanning and start codon recognition.
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_351622. Formation of the ternary complex, and subsequently, the 43S complex.

Miscellaneous databases

NextBioi967525.
PROiP0CX33.

Family and domain databases

InterProiIPR006846. Ribosomal_S30.
[Graphical view]
PfamiPF04758. Ribosomal_S30. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast homolog of mammalian ribosomal protein S30 is expressed from a duplicated gene without a ubiquitin-like protein fusion sequence. Evolutionary implications."
    Baker R.T., Williamson N.A., Wettenhall R.E.H.
    J. Biol. Chem. 271:13549-13555(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE, MASS SPECTROMETRY.
    Strain: YRB141.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  5. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND THR-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRS30A_YEAST
AccessioniPrimary (citable) accession number: P0CX33
Secondary accession number(s): D6VYT3, Q12087
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: June 28, 2011
Last modified: July 22, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 37600 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S30 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.